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515 HPLC
Waters 515 HPLC Manuals
Manuals and User Guides for Waters 515 HPLC. We have
1
Waters 515 HPLC manual available for free PDF download: Operator's Manual
Waters 515 HPLC Operator's Manual (130 pages)
Brand:
Waters
| Category:
Water Pump
| Size: 0 MB
Table of Contents
Copyright Notice
2
Trademarks
2
Customer Comments
3
Safety Considerations
4
Considerations Specific to the 515 HPLC Pump
4
Safety Advisories
4
Operating this Instrument
5
Applicable Symbols
5
Audience and Purpose
5
Intended Use of the Waters 515 HPLC Pump
5
Calibrating
5
Quality-Control
6
ISM Classification
6
EC Authorized Representative
7
Table of Contents
9
1 Waters 515 Pump Overview
14
Overview
14
Fluid-Handling Components
15
Electronics Components
16
Operating Modes
18
Stand-Alone Mode
18
Remote Control Mode
18
2 Installing the Waters 515 Pump
21
Site Requirements
22
Unpacking
23
Making Electrical Connections
24
Replacing the Power Supply Fuses
24
Connecting the Power Supply
25
Connecting for Remote Operation
26
Making Fluidic Connections
26
Connecting the Eluent Supply
26
Connecting the Pump Outlet
28
Connecting for High-Pressure Gradient Operation
31
3 Using the Waters 515 Pump
33
Powering on the Pump
34
Power-On Diagnostics
34
Diagnostics Failure
35
Keypad and Display Overview
35
Keypad
36
LCD Display
37
Editing or Entering Parameters in Local or Remote Modes
44
Preparing the 515 Pump
45
Priming the Pump
47
Purging the System
49
Equilibrating the System
50
Operating the Pump
53
Stand-Alone Pump Operation
53
Remote Pump Operation
54
Changing Parameters During a Run
56
Adjusting for Eluent Compressibility
56
Required Materials
57
Procedure
57
Powering off the Pump
58
4 Maintaining and Troubleshooting the Waters 515 Pump
59
Maintenance Considerations
60
Safety and Handling
60
Proper Operating Procedures
60
Spare Parts
60
Contacting Waters Technical Service
60
Diagnostic Tests
61
Retention Time Stability Monitoring
61
Performing the Ramp-And-Decay Test
61
Calibrating the Pump
63
Setting Zero Pressure
63
Setting the Head Size
64
Calibrating the Flow Rate
65
Resetting Strokes on Seals
66
Cleaning and Replacing Seals and Plungers
67
Replacing the Plunger Seals
67
Cleaning and Replacing the Plungers
71
Cleaning and Replacing Check Valves
76
Cleaning and Replacing the Inlet Check Valve
76
Replacing the Outlet Check Valve
79
Replacing the Draw-Off Valve
82
Required Materials
82
Removing the Draw-Off Valve
82
Installing the Draw-Off Valve
83
Troubleshooting
84
Troubleshooting Error Messages and Pump Malfunctions
85
Identifying and Correcting Noises
91
Identifying Chromatographic Problems
92
A Safety Advisories
98
Warning Symbols
98
Task-Specific Hazard Warnings
98
Specific Warnings
99
Caution Symbol
101
Warnings that Apply to All Waters Instruments
102
Electrical and Handling Symbols
108
Electrical Symbols
108
Handling Symbols
109
Specifications
111
Accessories and Spare Parts
115
Spare Parts
115
D Eluent Considerations
120
Introduction
120
Eluent Quality
120
Using Buffers or THF
120
Eluent Miscibility
120
How to Use Miscibility Numbers (M Numbers
122
Buffered Eluents
123
Eluent Viscosity
123
Eluent Degassing
123
Gas Solubility
124
Eluent Degassing Methods
125
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