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Waters 600E Installation And Maintenance Manual

Multisolvent delivery system
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  • Page 1 (217) 352-9330 | Click HERE Find the Waters 600E at our website:...
  • Page 2 Waters 600E Multisolvent Delivery System Installation and Maintenance Guide 34 Maple Street Milford, MA 01757 WAT174-03TP, Revision 3...
  • Page 3 This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, the use of this document.
  • Page 4 Note: When you use the instrument, follow generally accepted procedures for quality control and methods development. If you observe a change in the retention of a particular compound, in the resolution between two compounds, or in peak shape, immediately determine the reason for the changes.
  • Page 5 Caution: Use caution when working with any polymer tubing under pressure: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. • Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids.
  • Page 6 Attenzione: prestare attenzione durante l’utilizzo dei tubi di polimero pressurizzati: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Estinguere ogni fonte di ignizione circostante. • Non utilizzare tubi soggetti che hanno subito sollecitazioni eccessive o son stati incurvati.
  • Page 8 Caution: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention : L’utilisateur doit être informé que si le matériel est utilisé d’une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d’être défectueuses.
  • Page 9 Caution: To protect against fire hazard, replace fuses with those of the same type and rating. Attention : Remplacez toujours les fusibles par d’autres du même type et de la même puissance afin d’éviter tout risque d’incendie. Vorsicht: Zum Schutz gegen Feuergefahr die Sicherungen nur mit Sicherungen des gleichen Typs und Nennwertes ersetzen.
  • Page 10 Caution: To avoid possible electrical shock, disconnect the power cord before servicing the instrument. Attention : Afin d’éviter toute possibilité de commotion électrique, débranchez le cordon d’alimentation de la prise avant d’effectuer la maintenance de l’instrument. Vorsicht: Zur Vermeidung von Stromschlägen sollte das Gerät vor der Wartung vom Netz getrennt werden.
  • Page 11 Commonly Used Symbols Direct current Courant continu Gleichstrom Corrente continua Corriente continua Alternating current Courant alternatif Wechselstrom Corrente alternata Corriente alterna Protective conductor terminal Borne du conducteur de protection Schutzleiteranschluss Terminale di conduttore con protezione Borne del conductor de tierra...
  • Page 12 Commonly Used Symbols (Continued) Frame or chassis terminal Borne du cadre ou du châssis Rahmen- oder Chassisanschluss Terminale di struttura o telaio Borne de la estructura o del chasis Caution or refer to manual Attention ou reportez-vous au guide Vorsicht, oder lesen Sie das Handbuch Prestare attenzione o fare riferimento alla guida Actúe con precaución o consulte la guía Caution, hot surface or high temperature...
  • Page 13 Commonly Used Symbols (Continued) Caution, risk of electric shock (high voltage) Attention, risque de commotion électrique (haute tension) Vorsicht, Elektroschockgefahr (Hochspannung) Attenzione, rischio di scossa elettrica (alta tensione) Precaución, peligro de descarga eléctrica (alta tensión) Caution, risk of needle-stick puncture Attention, risques de perforation de la taille d’une aiguille Vorsicht, Gefahr einer Spritzenpunktierung Attenzione, rischio di puntura con ago...
  • Page 14 Commonly Used Symbols (Continued) Fuse Fusible Sicherung Fusibile Fusible Electrical power on Sous tension Netzschalter ein Alimentazione elettrica attivata Alimentación eléctrica conectada Electrical power off Hors tension Netzschalter aus Alimentazione elettrica disattivata Alimentación eléctrica desconectada...
  • Page 15 United States – FCC Emissions Notes This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
  • Page 16 600E Pump Information Intended Use When you develop methods, follow the “Protocol for the Adoption of Analytical Methods in the Clinical Chemistry Laboratory,” American Journal of Medical Technology, 44, 1, pages 30–37 (1978). This protocol covers good operating procedures and techniques necessary to validate system and method performance.

  • Page 18: Table Of Contents

    Selecting the Site Location................2 Determining Electrical Requirements ............... 4 1.3.1 System Power Requirements .............. 4 Chapter 2 Making Fluidic Connections to the 600E Pump ..........7 Selecting and Installing Fittings................ 7 2.1.1 Cutting and Deburring Stainless Steel Tubing ........8 2.1.2 Installing Connectors and Fittings ............
  • Page 19 Devices....................35 3.3.3 Setting IEEE-488 Addresses ............. 37 3.3.4 Performing IEEE-488 Powerup Sequence ........38 Making RS-232 Connections with the Waters 746 Data Module ....40 Making Screw Terminal Connections with External Devices......41 3.5.1 Screw Terminal Description ............. 41 3.5.2 Connecting a Non-IEEE-488 Autosampler ........44 3.5.3 Connecting a Non-IEEE-488 Detector ..........
  • Page 20 5.3.6 Performing the Chart Test ............... 100 5.3.7 Performing the Sparge Valves Test ..........101 5.3.8 Performing the Gradient Proportioning Valve Test ......101 Advanced 600E Pump Testing ..............102 5.4.1 Testing the Check Valves ..............102 5.4.2 Gradient Proportioning Valve Pair Test .......... 105...
  • Page 21 Troubleshooting Overview................109 Troubleshooting the 600E Pump..............111 6.2.1 System Pressure Overview ............. 112 6.2.2 System Pressure Flow Diagrams ............ 113 Troubleshooting the 600E System ..............119 Troubleshooting the Rheodyne 7725i Manual Injector......... 124 Appendix A Spare Parts ....................... 127 Index ...........................

  • Page 22: Preface

    Waters handles shipments, damages, claims, and returns. Online Help A convenient way to look up information while using the 600E System. You access Help by pressing the Help screen key whenever it appears on the controller screens.
  • Page 23 Waters 600E Multisolvent Delivery System User’s Guide: Provides an introduction to the features and use of the Waters 600E Multisolvent Delivery System. Documentation on the Web Related product information and documentation can be found on the World Wide Web.
  • Page 24 Notes Notes call out information that is helpful to the operator. For example: Note: Record your result before you proceed to the next step. Attentions Attentions provide information about preventing damage to the system or equipment. For example: Attention: To avoid damaging the detector flow cell, do not touch the flow cell STOP window.
  • Page 25 xxiv...

  • Page 26: Unpacking And Preparing Your System

    This chapter describes how to unpack and prepare your Waters 600E Multisolvent Delivery System for installation. 1.1 Unpacking and Inspecting The Waters 600E Multisolvent Delivery System is shipped in two containers. System components include: • 600 controller (electronics unit) •...

  • Page 27: Selecting The Site Location

    Immediately report any damage to both the shipping carrier and to your Technical Service Representative. You can contact Waters Technical Service at 800 252-4752, Canadian and U.S. customers only. Other customers, call your local Waters subsidiary or call Waters corporate headquarters for assistance in Milford, Massachusetts (U.S.A.).
  • Page 28 Waters 600E Multisolvent Delivery System (including detector, autosampler, and data system). Attention: Never stack fluidic components (such as the 600E pump or a STOP detector) on top of electronic devices without adequate leak protection.

  • Page 29: Determining Electrical Requirements

    1.3 Determining Electrical Requirements This section describes the electrical and system power requirements of the Waters 600E system. Caution: To avoid the possibility of electric shock, make sure the power cord is disconnected from the rear panel of the instrument before performing the procedures in this section.
  • Page 30 Table 1-1 Waters 600E System Electrical Specifications (Continued) Component Condition Specification Waters 600E pump Voltages input (provided by 12 VAC, 50/60 Hz controller) 12 VDC, 1.1 amps 30 VDC, 4.0 amps 5 VDC, 0.1 amps (600 controller) 15 VDC, 0.1 amps (600S...
  • Page 31 Unpacking and Preparing Your System...

  • Page 32: Making Fluidic Connections To The 600E Pump

    Chapter 2 Making Fluidic Connections to the 600E Pump This chapter describes the procedures for making fluidic connections to the 600E pump. 2.1 Selecting and Installing Fittings The Waters 600E Multisolvent Delivery System Startup Kit includes a variety of tubing and fittings to facilitate connections between the 600E pump and an external autosampler, column, and detector.

  • Page 33: Cutting And Deburring Stainless Steel Tubing

    Properties. Caution: Use of solvents that can damage the system could be hazardous to the operator and voids the 600E system warranty. Refer to the Waters 600E Multisolvent Delivery System User’s Guide, Appendix B, Eluent Considerations, for eluent compatibility information.
  • Page 34 Figure 2-1 Cutting Tubing with a Circular Cutter If you do not have a circular tubing cuter, use a knife-file to score the tube (Figure 2-2). Knife-File Figure 2-2 Cutting Tubing with a Knife-File Selecting and Installing Fittings...

  • Page 35: Installing Connectors And Fittings

    This section includes procedures for installing different types of connectors and fittings on tubing. This section covers: • Connector components • Assembling a standard ferrule connector • Assembling a reverse ferrule connector • Replacing ferrules Making Fluidic Connections to the 600E Pump...
  • Page 36 The ferrule is the component that seals a junction. When pressure is applied to a ferrule, the ferrule forms a seal against the junction surface by tightening the compression screw. The connectors supplied with your 600E system are standard ferrule connectors used with stainless steel tubing.

  • Page 37: Making 600E Pump Connections

    To replace a ferrule, cut off the old ferrule and continue from the start of the ferrule connection procedure. The compression screw may be reusable. 2.2 Making 600E Pump Connections This section describes 600E pump setup procedures: • Setting up the eluent reservoirs •...

  • Page 38: Setting Up The Eluent Reservoirs

    Figure 2-5 as a guide. Figure 2-5 600E Pump Connections 2.2.1 Setting Up the Eluent Reservoirs The 600E system includes the following components used in the setup of your eluent reservoirs: • Eluent reservoir caps • Diffuser filters for eluent and sparge tubing •...
  • Page 39 4-L bottle size. Figure 2-6 Eluent Reservoir Caps Choose eluent reservoirs which provide a snug fit for the reservoir caps. Waters recommends 1-L bottles. 1. Position the bottles in a convenient location, preferably at a higher level than the pump heads.
  • Page 40 The solvent and sparge tubing lines are on the right side of the pump near the front. 3. Uncoil the tubing and direct it around the right side of the 600E pump. Attention: Always flush new solvent filters and diffusers with methanol STOP before attaching the filters to the eluent and sparge tubing.
  • Page 41 Use particular care with volatile eluent in a cold room, refrigerator, or other small enclosed environment. 1. Cut a length of Teflon tubing (from the 600E Startup Kit) sufficient to reach a fume hood from the eluent reservoir. 2. Push one end of the tubing about one inch into the remaining hole in the reservoir cap.

  • Page 42: Setting Up The Sparge System

    2.2.2 Setting Up the Sparge System This section describes how to connect a helium tank to the 600E pump to sparge the eluent reservoirs. This section covers: • System sparging overview • Attaching compression fittings to the helium line •...
  • Page 43 • Total water less than 1.0 Mppm • Total hydrocarbon less than 0.5 Mppm Attention: The 600E system warranty may be voided if you use an inferior STOP grade of helium gas. Attaching Compression Fittings to the Helium Line Stainless steel tubing (1/16-inch, with at least 0.020-inch ID, 0.040-inch ID preferred) connects the helium supply to the 600E pump.

  • Page 44: Installing A Column Or Cartridge

    Subsequent connections with this fitting do not require the extra 3/4 of a turn. 1/8 of a turn is normally adequate. Connecting the Helium Sparge Supply 1. Connect the tubing from the helium supply to the sparge gas inlet on the 600E pump rear panel (Figure 2-9) using the tubing and compression fittings assembled above.

  • Page 45: Installing A Column

    Note: For information on solvent selection and equilibration before use, refer to the Care and Use Manual supplied with your column or cartridge. Observe precautions during use. 2.3.1 Installing a Column To install a column in the 600E pump slide-out drawer: 1. Turn the latch on the front of the pump (see Figure 2-5) and pull out the slide-out drawer.
  • Page 46 3. Place the column in the tray with the arrow on the column label pointing toward the front of the drawer. Allow the column to lie flat in the drawer (Figure 2-11). 4. Attach the inlet tubing and outlet tubing to the column as described below (Figure 2-11).

  • Page 47: Installing A Column Heater

    3. Pull the drawer toward you and locate the receptacle at the back of the drawer (Figure 2-13). Plug the signal cable into the receptacle on the right side of the drawer. The receptacle is keyed to ensure correct alignment. Making Fluidic Connections to the 600E Pump...
  • Page 48 Receptacle Drawer Pulls Out Figure 2-13 Column Heater Signal Cable Connection 4. Lift the column heater covers straight off the column heater (Figure 2-14). Place your column (up to 8 mm ID by 30 cm) in the center channel of the column heater tray with the arrow on the column label pointing toward the front of the drawer.

  • Page 49: Installing An Rcm 8 X 10

    RCM 8 x 10. • Cartridge Outlet – Using a union, attach a piece of 0.009-inch ID tubing to the column outlet at the front of the RCM 8 x 10. Making Fluidic Connections to the 600E Pump...

  • Page 50: Making Fluidic Connections To An Autosampler

    For detailed operation information, refer to your Waters RCM 8 x 10 Operator’s Manual. 2.4 Making Fluidic Connections to an Autosampler You have the option to use the Waters 600E Multisolvent Delivery System with an external autosampler. You can: •...
  • Page 51 Cartridge). Connecting an Autosampler in Place of the Rheodyne 7725i Manual Injector 1. Place the autosampler on the selected side of the system (Figure 2-16). Allow room for access to the eluent reservoirs. Making Fluidic Connections to the 600E Pump...

  • Page 52: Making Fluidic Connections To A Detector

    Connect the autosampler inlet line with a union to the transducer outlet line. 2.5 Making Fluidic Connections to a Detector To connect the 600E pump to your detector inlet: 1. Pass the column or cartridge outlet tubing through the notch in the pump drawer front panel.
  • Page 53 Making Fluidic Connections to the 600E Pump...

  • Page 54: Making Electrical Connections To The 600 Controller

    Chapter 3 Making Electrical Connections to the 600 Controller This chapter describes the electrical connections you make to the 600 controller rear panel. 3.1 Controller Rear Panel Overview The 600 controller rear panel allows connection to: • AC power connector •...
  • Page 55 Figure 3-1 600 Controller Rear Panel Connectors AC Power Connector Provides electrical power to the 600E system. For details on connecting AC power, refer Section 3.2, Attaching the Pump Interface Cable and Power Cord. Pump Interface Connector Enables the 600 controller and 600E pump to communicate with each other. The pump interface connector also provides power to the 600E pump.

  • Page 56: Attaching The Pump Interface Cable And Power Cord

    Making Screw Terminal Connections with External Devices. 3.2 Attaching the Pump Interface Cable and Power Cord This section covers the basic connections required to use the 600E system. It covers: • Attaching the pump interface cable • Attaching the AC power cord...
  • Page 57 (Figure 3-2). 3. Connect the other end of the 37-pin interface cable to the rear of the 600E pump. Figure 3-2 Pump Interface Cable and Power Cord Locations Attaching the AC Power Cord 1. Insert the D-shaped connector end of the power cord into the power receptacle on...

  • Page 58: Making Ieee-488 Interface Connections

    Multisolvent Delivery System User’s Guide, Section 8.1, Setting Up the 600 as a Gradient Controller. 1. Connect the single receptacle end of the IEEE-488 cable (supplied with either the 600E system or the Waters data system) to your data system and attach the cable to the busLAC/E (Laboratory Acquisition and Control/Environment) card (Figure 3-3).
  • Page 59 Empower Client Workstation 2487 UV Figure 3-4 IEEE-488 Connections in a Waters 845/860 System 4. Repeat steps 2 and 3 for each additional component. Note: The maximum total cable length between IEEE-488 devices is 65 feet (20 meters). The maximum recommended cable length between two IEEE-488 devices is 13 feet (4 meters).

  • Page 60: Making Ieee-488 Connections With External Powerline Devices

    IEEE-488 interface). Set up the 600 as a PowerLine Controller. Note: To set up the 600 controller as a PowerLine Controller, refer to the Waters 600E Multisolvent Delivery System User’s Guide, Section 4.1, Setting Up the 600 as a PowerLine Controller.
  • Page 61 Sequence. Attaching a Waters 432 Detector to a PowerLine System In the PowerLine mode, the 600 PowerLine Controller supports operation with a Waters 432 conductivity detector through an interface box. You must use the interface box to communicate with the 432 detector over the IEEE-488 interface. When operating with the...

  • Page 62: Setting Ieee-488 Addresses

    • When using the 600 controller as a PowerLine Controller, you do not set the IEEE-488 address of the 600E pump. In the PowerLine system configuration, you set the IEEE-488 address of each of the other PowerLine instruments through the instrument software or DIP switches.

  • Page 63: Performing Ieee-488 Powerup Sequence

    2. Power up all equipment controlled by the data system that is not under direct IEEE-488 control. 3. Power up the 600E pump to establish eluent flow. Then power up all other equipment controlled through the IEEE-488 bus. Making Electrical Connections to the 600 Controller...
  • Page 64 3. Power up the 600 controller. Power Down Sequence for IEEE-488 Devices If you do not plan to use the Waters 600E system for a long period of time (overnight or longer), power down the system. Note: For a list of power-down precautions for storing the 600E pump, refer to the Waters 600E Multisolvent Delivery System User’s Guide, Chapter 3, Preparing Your 600E...

  • Page 65: Making Rs-232 Connections With The Waters 746 Data Module

    3.4 Making RS-232 Connections with the Waters 746 Data Module The RS-232 interface connects the Waters 600 controller to a Waters 746 data module. The interface transmits: • Control commands from the data module to the controller • Commands and report information from the controller to the data module No chromatographic data is transmitted across the interface.

  • Page 66: Making Screw Terminal Connections With External Devices

    3.5.1 Screw Terminal Description The screw terminals of the 600 controller enable the controller to receive signals from or generate signals to external devices (for example, a non-IEEE-488 autosampler, a Waters 746 Data Module, or an automated switching valve). Depending on the external devices within your particular system, use the appropriate...
  • Page 67 Receives a signal from an external injector (autosampler or Rheo- (Digital Input) dyne 7725i manual injector) to initiate the chromatographic run. The Inject terminal also provides a signal to start a Waters 746 Data Module. The Inject terminal accepts signals from several outputs, including TTL signals, open collector outputs, or contact closures.
  • Page 68 0 to 10 mV full scale. Pressure Terminals - Transmit a voltage representation of the current 600E system backpressure. This is a direct pressure trace from the pump transducer (0 to 6000 psi is full scale), where 10 mV = 6000 psi.

  • Page 69: Connecting A Non-Ieee-488 Autosampler

    3.5.2 Connecting a Non-IEEE-488 Autosampler Background When you use a non-IEEE-488 autosampler or a Waters IEEE-488 autosampler in stand-alone mode, you must connect an inject start signal to the 600 controller. The 600 controller requires an electrical trigger signal from the injector (manual injector or autosampler) at the Inject terminal as each injection occurs.
  • Page 70 Figure 3-8 illustrates connecting a non-IEEE-488 autosampler to the Inject terminal. Attention: Be careful to maintain trigger cable polarity between devices in order not to STOP close the inject start circuit. If you cross the trigger cables, some devices may not receive the inject start signal.

  • Page 71: Connecting A Non-Ieee-488 Detector

    Connecting the Inject Signal to the Waters 746 You must connect a run start signal to the Waters 746 to instruct the device when to initiate data collection. The Waters 746 receives the run start signal in one of two ways: •...
  • Page 72 (Figure 3-10). Note: When you connect the Waters 746 to the 600 controller, you must also attach an RS-232 interface cable between the 600 controller and the 746. Refer to Section 3.4, Making RS-232 Connections with the Waters 746 Data Module.
  • Page 73 Figure 3-9 Connecting the Waters 746 to the 600 Controller Inject Terminal Making Electrical Connections to the 600 Controller...
  • Page 74 Figure 3-10 Connecting the Waters 746 and Autosampler to the Inject Terminal Connecting the Pressure or Chart Terminals to the Waters 746 You can use the second channel on your Waters 746 to monitor one of the following pump output signals.
  • Page 75 Waters 600E Multisolvent Delivery System User’s Guide). Connections To monitor the analog output signal from either the Pressure or Chart terminals, connect the event cable (provided in the 600E Startup Kit) as described below: 600 Controller Monitor Function Waters 746...

  • Page 76: Connecting A Chart Recorder

    Figure 3-12 Connecting the Waters 746 to the 600 Controller Chart Terminals 3.5.5 Connecting a Chart Recorder Background You can use the second pen on your chart recorder to monitor one of the following pump output signals: • 600E pump pressure during ramp-up testing (from Pressure terminals) •...
  • Page 77 Connections To monitor the analog output signal from either the Pressure or Chart terminals, connect the event cable (provided in the 600E Startup Kit) as outlined below: 600 Controller Monitor Function Chart Recorder Connection Pump Pressure Pressure + and –...

  • Page 78: Connecting An External Device

    Figure 3-14 Connecting the Chart Recorder to the 600 Controller Chart Terminals 3.5.6 Connecting an External Device Background When your system includes an external TTL-level or contact closure device, you can use switches S1 through S4 on the 600 controller to control external devices. External devices can include: •...
  • Page 79 • Isocratic or Direct Control screen (for immediate activation) Refer to Section 5.1 or 6.2 of the 600E Multisolvent Delivery System User’s Guide. Connections To control an external device using one of the S1 through S4 screw terminals, connect the...
  • Page 80 To attach the external device to the Aux +12 V terminal, attach the event cable (provided in the 600E Multisolvent Delivery System Startup Kit) as outlined below: 600 Controller Connection External Device Aux +12 V S1, S2, S3, or S4 –...
  • Page 81 Making Electrical Connections to the 600 Controller...

  • Page 82: Maintenance Procedures

    Caution: To prevent the possibility of electric shock, never disconnect an electrical assembly (including the pump interface cable) while power is applied to the Waters 600E system. Attention: To prevent damage to the 600 controller, wait approximately three minutes...

  • Page 83: Maintaining 600E Pump Components

    Caution: To avoid the possibility of electric shock, do not open the 600E controller cover. The 600E controller does not contain user-serviceable components. Performing 600E System Operating Procedures To keep your 600E system running smoothly, follow the operating procedures and...

  • Page 84: Pump Overview

    Caution: When handling eluents, changing tubing, or operating the 600E system in general, always observe good laboratory practices. Know the physical and chemical properties of the eluents. Refer to the Material Safety Data Sheets for the eluents in use. This section covers: •...
  • Page 85 Figure 4-1 600E Pump Assemblies Table 4-1 600E Pump Assemblies Description Function Inlet manifold valve Eluent purging, pump priming (injection), eluent draw-off. Pump head assemblies Draw in/expel eluent. (left and right) Drain holes (behind inlet Drain solvent that collects behind the plunger seals (position check valves) on left head shown).

  • Page 86: Calibrating And Replacing The Pressure Transducer

    Table 4-1 600E Pump Assemblies (Continued) Description Function Vent holes (top of Provide access for flushing residue from the backs of the flange) plungers (positions on right head shown). Reference valve Assists pump priming. Used with detectors requiring refer- ence solvent stream.
  • Page 87 Figure 4-2 Transducer Fittings and Zero Adjust Screw Replacing the Transducer 1. Power down the 600 controller and disconnect the power cord. 2. Remove the retaining screws from the upper rear panel of the pump and from both sides of the front panel. Slide the cover off. 3.
  • Page 88 Figure 4-4 Transducer Connection on Interconnect Board 7. Remove the transducer and spacer from behind the front panel. Installing a New Transducer 1. Line up the spacer and transducer and replace the two screws on the front panel. Maintaining 600E Pump Components...

  • Page 89: Removing The Pump Head

    3. Replace the cover of the pump and connect the power cord. 4. Check the calibration adjustment of the transducer. 4.2.3 Removing the Pump Head Note: For information about isolating problems in the 600E pump, see Chapter 6, Troubleshooting. Figure 4-5 is an exploded diagram of a pump head.

  • Page 90: Replacing The Plunger Seal

    Figure 4-6 Pump Head Removal 4.2.4 Replacing the Plunger Seal This section describes replacing the pump plunger seal. For information about isolating plunger problems in the 600E pump, see Chapter 6, Troubleshooting. Note: To ensure optimum pump performance, replace the seals in both pump heads (instead of only replacing the seal on one pump head).

  • Page 91: Cleaning And Replacing The Pump Plunger

    Check for leaks. If there is a leak, verify pump head and plunger seal installation. Note: For priming information, see the Waters 600E Multisolvent Delivery System User’s Guide, Chapter 3, Preparing Your System for Operation.
  • Page 92 Note: For information about isolating plunger problems in the 600E pump, see Chapter 6, Troubleshooting. If you do not need to disassemble the pump head, you can remove the complete pump head-plunger support assembly as a unit. To remove the pump head assembly as a unit, omit step 2.
  • Page 93 Figure 4-9 Plunger Disassembly Cleaning the Plunger 7. Inspect the plunger for damage, excessive wear, or mobile-phase debris. (You may see lateral or rotational scratches and solvent residue.) If the plunger seal has excessive wear: • Clean the plunger with a fine pumice to remove any coating. •...

  • Page 94: Cleaning And Replacing Pump Check Valves

    Removing an outlet check valve • Installing an outlet check valve Note: For information about isolating check valve problems in the 600E pump, see Section 5.4.1, Testing the Check Valves. Whenever you suspect a check valve of contributing to faulty pump operation, loosen and then retighten the check valve housings.
  • Page 95 Figure 4-10 Inlet Check Valve Removal Cleaning the Inlet Check Valve 7. Check the retainer and the ball and seat for damage or debris (Figure 4-11). Clean or replace worn parts as necessary. The housing seal, which may be difficult to remove, does not normally need to be replaced.
  • Page 96 4-12). Disassemble the check valve by tipping the housing upside down. 9. Check the retainer and ball and seal (Figure 4-13) for damage or debris. The housing seal, which may be difficult to remove, does not normally require replacement. Maintaining 600E Pump Components...
  • Page 97 Figure 4-12 Outlet Check Valve Removal Cleaning the Outlet Check Valve Clean the check valve by sonicating in water, followed by sonicating in methanol. Replace worn or damaged parts as necessary. Installing the Outlet Check Valve If there is no improvement, install a new outlet check valve: 1.
  • Page 98 8. Place the third rulon washer on top of the cup filter. 9. Turn the pump head upside down and screw in the outlet check housing. 10. Hand tighten the assembly. 11. Replace the pump head. 12. Tighten the check valve assembly to seal. Maintaining 600E Pump Components...

  • Page 99: Replacing Fuses

    • Operating voltage fuse (on the 600 controller rear panel) • Auxiliary +12 V and 600E pump power fuses (on the 600 controller rear panel) • Column heater power fuse (on the pump interconnect board) 4.3.1 Replacing the Operating Voltage Fuse When to Replace the Operating Voltage Fuse The 600 controller is supplied with two 2–A fuses installed for operation.
  • Page 100 3. To change the AC power fuses, pull the red fuse holder as though opening a drawer. Spare fuses are included in the 600E Multisolvent Delivery System Startup Kit (Figure 4-14). For ordering information, see Appendix A, Spare Parts. Figure 4-14 Removing the Fuse Holder 4.

  • Page 101: Replacing Auxiliary +12 V And Pump Fuses

    Auxiliary +12 V power fuse • 600E pump power fuse Note: For information about isolating power problems in the 600E pump, see Chapter 6, Troubleshooting. The auxiliary +12 V fuse protects the AUX +12 V terminal on the 600 controller rear panel from excessive current loads.
  • Page 102 6. Lock the fuse holder into place using the screwdriver. Replacing the 600E Pump Power Fuse The 600E pump power fuse protects the 600E pump from excessive current loads. If this fuse is blown, no power is available at the 600E pump.

  • Page 103: Replacing The Column Heater Power Fuse

    Caution: To avoid the possibility of electric shock, turn off the 600 controller front panel power switch and unplug the power cord from the rear panel. 2. Loosen the two pump cover retaining screws at the rear of the 600E pump. Lift the cover off the pump.

  • Page 104: Maintaining The Rheodyne 7725I Manual Injector

    4.4 Maintaining the Rheodyne 7725i Manual Injector Note: The Rheodyne 7725i manual injector is optional. If it is not installed on your system, ignore this section. The Rheodyne 7725i manual injector requires minimal maintenance. With normal use, the injector should perform thousands of cycles without fail. This section describes the following maintenance procedures: •...

  • Page 105: Replacing The Position Sensing Switch

    4.4.2 Replacing the Position Sensing Switch The position sensing switch is a magnetic reed switch actuated by a magnet in the shaft. The switch sends the inject signal to the controller, signalling the controller to begin a gradient. If a gradient does not begin after an injection, replace the switch. The switch is rated for 100 V at 200 mA.

  • Page 106: Rotor Seal Leakage

    4.4.3 Rotor Seal Leakage If you observe liquid leaking between the stator and stator ring, tighten the pressure adjusting screw. If you observe liquid leaking from the needle port or vent tube, replace the rotor seal. 4.4.4 Tightening the Pressure Adjusting Screw 1.

  • Page 107: Reassembling The Injector

    When replacing the rotor seal, always check the ceramic stator face for chips or cracks. If the stator face is damaged, replace it. To replace the rotor seal (refer to Figure 4-19): 1. Leave the injector attached to the front panel of the pump, and leave the knob on. If you need to remove the injector from the front panel, remove the knob.
  • Page 108 • Tighten each of the three stator screws a little at a time to keep the stator surface parallel to the stator ring surface until all parts are held firmly in place. • Retighten the pressure adjusting screw until the red dots align as noted in step 1. •...
  • Page 109 Maintenance Procedures...

  • Page 110: Error Messages, Diagnostics, And Test Procedures

    (including the pump interface cable) while power is applied to the Waters 600E system. Caution: To avoid the possibility of electric shock, do not open the 600E controller cover. The 600E controller does not contain user-serviceable components. Caution: When handling eluents, changing tubing, or operating the 600E system in general, always observe good laboratory practices.

  • Page 111: Summary Of Error Messages

    • Use an unacceptable entry method Warning messages typically instruct you to correct an error made during programming or operation of the 600E system. For a list of warning messages and information about correcting problems, see Section 5.1.2, Warning Messages.

  • Page 112: Warning Messages

    5.1.2 Warning Messages Overview Warning messages appear on the 600 controller screen to instruct you to: • Enter acceptable values • Check your entries Pay attention to warning messages and take corrective action. Failure to do so may cause the 600 controller to produce erroneous results or to fail to perform your chromatographic run.
  • Page 113 Table 5-1 Warning Messages (Continued) Message Displayed Message Appears Meaning When programming The composition values for %A, Composition MUST eluent composition %B, %C, and %D do not total sum to 100% 100%. Check your values for all eluent reservoirs. While modifying a The table currently in memory and Enter Table Gradient, Event, or...
  • Page 114 Detector table should not be erased. 1 = Yes 0 = No After 600 controller Suspend 600E system operation Rear Panel Stop receives a signal at the until the source of the Stop Flow Flow Input Stop Flow terminal signal is removed.
  • Page 115 Table 5-1 Warning Messages (Continued) Message Displayed Message Appears Meaning During operation in the The 410 detector has disappeared RI Detector Not Direct Control or from the IEEE-488 bus. Verify if Found Operate Methods the 410 detector: screen • Is turned on •...

  • Page 116: Shutdown Messages

    Help key for a summary of allow- able ranges. 5.1.3 Shutdown Messages Overview Shutdown messages appear on the 600 controller screen when the 600E pump: • Exceeds an operator-set limit • Receives a Stop Flow signal from an external device...
  • Page 117 1. Press Clear to clear the message from the screen. 2. Check the Pump Setup screen parameters and make any adjustments to the entered values (for details, refer to the Waters 600E Multisolvent Delivery System User’s Guide). 3. Determine if the 600 controller received a Stop Flow signal from an external device.
  • Page 118 If the problem continues, notify Waters Technical Service. You may contact Waters Technical Service at 800 252-4752, Canadian and U.S. customers only. Other customers, call your local Waters subsidiary or call Waters corporate headquarters for assistance in Milford, Massachusetts (U.S.A.).

  • Page 119: Running 600 Controller Self-Diagnostic Tests

    Pump Configuration screen. If the screen pump head volume value is incorrect for the 600E pump, a mismatch in flow rate occurs, resulting in a shutdown. If the Pump Head Vol. value is correct, there is probably an internal failure (such as in pump drive circuits and pump mechanics).
  • Page 120 If the screen displays no results, the controller failure may be in the LCD display circuitry. Restarting To restart the program, turn the power switch off. Wait 10 seconds, and then turn the power switch back on. If characters fail to appear a second time, notify Waters Technical Service. Contacting Technical Service If the 600E system fails any of the self-diagnostic tests, contact Waters Technical Service at 800 252-4752, Canadian and U.S.

  • Page 121: Performing 600 Controller Extended Test Routines

    When to Run Extended Diagnostic Tests Perform the extended test routines if you believe you are having a problem with the Waters 600 controller and want to narrow down the malfunctioning area. After the automatic self-diagnostic routine finishes (refer to Section 5.2, Running 600...
  • Page 122 If this condition is detected, the test passes. Counter test Verifies that the valves cannot switch to a 50/50 condition. If any tests fail during this procedure, notify Waters Technical Service. Required Tools Tools required for the extended test routines are: •...
  • Page 123 Contacting Technical Service If the 600E system fails any of the extended tests, contact Waters Technical Service at 800 252-4752, Canadian and U.S. customers only. Other customers, call your local Waters subsidiary or call Waters corporate headquarters for assistance in Milford, Massachusetts (U.S.A.).

  • Page 124: Performing The Stop Flow Test

    5.3.2 Performing the Stop Flow Test The Stop Flow switch accepts a signal from an external device that stops the pump. This test verifies that the Stop Flow input switch is functioning. 1. Attach a jumper wire from the Stop Flow terminal to the Gnd terminal on the 600 controller rear panel (see Figure 5-2).

  • Page 125: Performing The Hold Switch Test

    2. Press Enter. • If the test is successful, Test Passed appears on the screen. • If the test fails, Test Failed appears on the screen. 3. To exit the test, press Enter. 4. Repeat steps 1 through 3 for the remaining switch terminals (S2 through S4). 5.

  • Page 126: Performing The Sparge Valves Test

    5.3.7 Performing the Sparge Valves Test This test identifies whether a malfunctioning sparge line is due to electronics or sparge-valve failure. Perform this test if you do not see any bubbles when the 600E system sparges eluents. 1. Press Enter.

  • Page 127: Advanced 600E Pump Testing

    2. Open the reference valve to release pressure in the system, or allow pressure to decay to zero. 3. Flush the 600E pump with an eluent that is miscible with methanol (used in step 7). To remove any buffers, flush with water, followed by methanol.
  • Page 128 Table 5-4 Pressure Trace Recording Pressure Recording Example Condition Normal pump operation Check valve holding Forward right plunger 92% of full stroke scale Failing outlet check valve Check valve leaking Forward right plunger 92% of full stroke scale Advanced 600E Pump Testing 103...
  • Page 129 Table 5-4 Pressure Trace Recording (Continued) Pressure Recording Example Condition Failed outlet check valve Check valve leaking Forward right plunger stroke 92% of full scale Failing inlet check valve Forward right plunger 92% of full stroke scale Failed inlet check valve No pressure from plunger stroke...

  • Page 130: Gradient Proportioning Valve Pair Test

    Flushing the Eluent Lines Be sure to flush any previous eluents from the system and change over thoroughly to the test eluents (see Waters 600E Multisolvent Delivery System User’s Guide, Chapter 3, Preparing Your 600E Multisolvent Delivery System for Operation).
  • Page 131 100% composition (methanol containing propylparaben in eluent 2) = 100% full scale. Entering the Recommended Gradient Table Enter and save the following gradient table (see the Waters 600E Multisolvent Delivery System User’s Guide for details about entering a gradient table). Time...
  • Page 132 Valve A) will be irregular when compared to Peaks 3 and 4. Similarly, if Valve B is malfunctioning, Peaks 3 and 4 will be irregular. Contacting Technical Service If the 600E system fails the test, contact Waters Technical Service. Advanced 600E Pump Testing 107...
  • Page 133 Error Messages, Diagnostics, and Test Procedures...

  • Page 134: Chapter 6 Troubleshooting

    Caution: To prevent the possibility of electric shock, never disconnect an electrical assembly (including the pump interface cable) while power is applied to the Waters 600E system. Attention: To prevent damage to the 600E controller, wait approximately three minutes...
  • Page 135 5. Run the performance tests for each chromatographic instrument to quickly determine if a problem exists with the instrument. Where to Go for Help If you determine that there is a problem related to the 600E system, refer to: • Section 6.2, Troubleshooting the 600E Pump •...

  • Page 136: Troubleshooting The 600E Pump

    If you need to perform additional testing of the 600E pump to determine the potential source of a problem, refer to Section 5.4, Advanced 600E Pump Testing. If you determine that there is a problem related to another system component, refer to the appropriate operator’s guide.

  • Page 137: System Pressure Overview

    • Noting gradual versus sudden pressure increase • Isolating high pressure locations in the 600E system Determining Your System Pressure Reference Point To identify a pressure change from normal operation, it is critical that you have a pressure reference point. System pressure is affected by the column, flow rate, mobile phase, and temperature, and can vary greatly with different methods.

  • Page 138: System Pressure Flow Diagrams

    This section includes flow diagrams for isolating the source of system pressure problem. It includes: • High system pressure troubleshooting (Figure 6-1) • Low system pressure troubleshooting (Figure 6-2) • Erratic system pressure troubleshooting (Figure 6-3) Troubleshooting the 600E Pump 113...
  • Page 139 High Check Operating Temperature. If too Low, Stabilize Has Ambient Temperature Changed? Operating Environment. Set to Correct Flow Rate (See 600E Multisolvent Is Pump Flow Rate Set too High? Delivery System User’s Guide). Check Eluent Viscosity. Observed Pressure Can Be Are Highly Viscous Eluents Being Normal for the Column and Solvent Blend.
  • Page 140 Blocked Outlet Tubing. Clean or Replace Tubing Loosen Fitting at Pump (See Section 2.1). Check Eluent Miscibility (See Outlet. Pressure 600E Multisolvent Delivery System User’s Guide, Reduced? Appendix B). Pump Problem. Call Waters Technical Service. Figure 6-1 High System Pressure Troubleshooting (Continued)
  • Page 141 Outlet? Inspect for Leaks and Measure Flow in Detector. Set to Correct Limit (See Is the Low Pressure 600E Multisolvent Limit Set Higher than Delivery System User’s Operating Pressure? Guide). Prime the Pump (See Is There Air in the Eluent...
  • Page 142 Figure 6-2 Continued from Previous Page Set to Correct Flow Rate (See 600E Multisolvent Is Pump Flow Rate Set Delivery System User’s Guide). too Low? Check Operating Temperature. If Temperature Is Has Ambient too High, Stabilize Operating Environment. Temperature Changed? Are There Any Visible Tighten Any Loose Fittings (Do Not Overtighten).
  • Page 143 Erratic System Pressure Is the Transducer Set Flow Rate to 0. Is Defective Transducer. Call Operating Properly? Pressure Stable? Waters Technical Service. Prime the Pump (See 600E Multisolvent Is the Pump Operating Pump Problem. Delivery System User’s Guide, Properly? Section 6.3.

  • Page 144: Troubleshooting The 600E System

    Table 6-1 is a guide to troubleshooting pump problems. It lists pump-related symptoms, possible causes, and corrective action. To perform additional testing of the 600E pump to determine the potential source of a problem, refer to Section 5.4, Advanced 600E Pump Testing.
  • Page 145 Attention: Never disconnect the pump from the controller while the two devices are powered on. Pump low pressure Set to the correct pressure limit (see the Waters 600E limit set to a value Multisolvent Delivery System User’s Guide). that is higher than...
  • Page 146 Table 6-1 600E System Troubleshooting (Continued) Symptom Possible Cause Corrective Action Pump not deliv- Damaged plunger Check for eluent leaking from behind the pump head. ering eluent seal This is an indicator of a damaged plunger seal. (continued) Check if both pump heads maintain pressure as outlined in the following section.
  • Page 147 Check eluent level in reservoirs. Refill as necessary, of eluent and degas/helium-sparge. Air bubble in pump Prime pump to remove bubble (see the Waters 600E head Multisolvent Delivery System User’s Guide, Chapter 3). Ensure there are no air bubbles in the eluent inlet lines.
  • Page 148 Table 6-1 600E System Troubleshooting (Continued) Symptom Possible Cause Corrective Action Erratic flow Dirty or malfunc- To determine if the symptom is due to a problem rate/pump pulsa- tioning check with the inlet or outlet check valve, refer to tions valve(s) Section 5.4, Advanced 600E Pump...

  • Page 149: Troubleshooting The Rheodyne 7725I Manual Injector

    Contact Waters Technical Service. (continued) connected. High system pres- Pump flow rate set Set to the correct operating flow rate (see the Waters sure due to pump too high 600E Multisolvent Delivery System User’s Guide). Pressure transducer Contact Waters Technical Service.
  • Page 150 Table 6-2 Rheodyne 7725i Injector Troubleshooting (Continued) Symptom Possible Cause Corrective Action Rotor seal leakage Pressure adjusting Tighten pressure adjusting screw. Refer to (liquid dripping screw loose Section 4.4.4, Tightening the Pressure between stator and Adjusting Screw. stator ring, or from Scratches on rotor seal Replace rotor seal.
  • Page 151 Troubleshooting...

  • Page 152: Appendix A Spare Parts

    Appendix A Spare Parts The following spare parts are recommended for customer installation. Any parts not listed Table A-1 may require installation by a trained service representative. Table A-1 Pump Replacement Parts Item Part Number Bottle, 1 L WAT032750 Bottle caps, 1 L WAT062479 Bottle caps, 4 L WAT062341...
  • Page 153 Table A-1 Pump Replacement Parts (Continued) Item Part Number Fuse, 1.5 A, 250 V, 5 x 20 mm, UL/CSA, fast, 12 V WAT055632 external, North America, 5/pkg Fuse, 2 A, 250 V, 5 x 20 mm, UL/CSA, time delay, WAT055628 power primary at 100/120 VAC, 5/pkg, North America Fuse, 2.5 A, 250 V, for column heater...
  • Page 154 WAT062060 Rheodyne manual injector (with 600 mounting WAT024838 plate) Slotted nut driver, 5/16, modified WAT076374 Sparge valve WAT062010 Startup Kit, 600E WAT055979 Syringe, 25 µL WAT033381 Syringe, safety, 10 mL WAT027629 Tubing, stainless steel, 0.062 x 0.020 x 60-inch WAT025592 Tubing, stainless steel, 0.062 x 0.040 x 120-inch...

  • Page 156: Index

    Aux +12 V signal IEEE-488 addresses description Millennium 2010 IEEE-488 cabling external device cabling powerup/power down sequence maximum current Waters 746 RS-232 cabling Auxiliary +12 V power fuse, replacing Dead volume, minimizing 7, Detector additional electrical connections column connections 21, fluidic connections...
  • Page 157 Eluent lines Fittings, attaching connecting to reservoir Fume hood, using locating Fuses Eluent proportioning valve 600E power fuse, replacing Eluent Proportioning Valve test auxiliary +12 V fuse, replacing pair test operating voltage fuse, replacing Eluent reservoirs replacing connecting to fume hood...
  • Page 158 Low pressure procedure Plunger seal, replacing Power down sequence Maintenance Powerup sequence 600E pump power fuse, replacing data system auxiliary +12 V power fuse, replacing PowerLine Controller Pressure (+/–) signal inlet check valve connection to chart recorder operating voltage fuse, replacing...
  • Page 159 44, extended test troubleshooting external device cabling Rotor seal, replacement System interface RS-232 cabling cabling to Waters 746 connector communication System power requirements connector System pressure erratic pressure 111, factors affecting high pressure 111, 112, Safety and handling warnings 57,...
  • Page 160 Tubing, considerations Inject signal connection Pressure signal connection RS-232 cabling Waters Technical Service 58, 93, 95, 98, U6K injector attaching to column 21, attaching to RCM 8 x 10 in series with autosampler Unpacking and inspection...
  • Page 161 Index...

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