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Caution: Always follow safe laboratory practices when handling eluents. Know the
physical and chemical properties of the eluents. See the Material Safety Data Sheets for
the eluents in use.
Caution: When using sample loops larger than 100-µL, protect yourself from the rapid
ejection of mobile phase from the needle port when you turn the valve from Inject to
Load. A-1 mL loop expands by 20-µL upon decompression from 2900 psi (200 bar).
Attention: Be sure to use the proper size of syringe needle to avoid damage to the valve.
STOP
Proper needle dimensions are 0.028 inches x 2 inches (0.071 cm x 5.08 cm) OD, without
electro taper. The point must be 90 ° (square).
To load the sample into the Rheodyne 7725i:
1. Turn the handle on the manual injector to the Load position
Figure 11-1 Rheodyne 7725i Injector in the Load Position
2. Load a syringe with sample. The loop may be partially filled or overfilled. The
injector is equipped with a 20-µl loop.
3. Fully insert the 22-gauge, 90° point syringe needle into the filler port.
4. Gently discharge the contents of the syringe.
5. Turn the Load/Inject handle to the Inject position.
190
Running Gradients
(Figure
11-1).
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