Starting A Run; Changing Parameters While Running; Stopping The Pump - Waters 600e User Manual

9.2.2 Starting a Run

Using the Rheodyne 7725i Manual Injector
1. Load the injector loop with sample. See
Manual
2. Turn the injector handle to the Inject position to make the injection.
Using a Non-PowerLine Autoinjector
Note: If you are using an autoinjector, see the documentation provided with that product
for information on sample loading and operation.
To begin a run with an external non-PowerLine autoinjector:
1. Load the sample vials into the autoinjector.
2. Program and start the autoinjector. The autoinjector makes the injection.
Completing the Run
In the isocratic mode, there is no run-time clock, no system status, and no change in eluent
composition. The run continues until you stop the pump. See
Pump.

9.2.3 Changing Parameters While Running

The pump and external event switch parameters displayed on the Isocratic screen can be
changed while a run is in progress.
To modify a parameter while a run is in progress:
1. Move the cursor to the appropriate field.
2. Type the new value and press Enter. The change takes effect immediately.

9.2.4 Stopping the Pump

To stop the pump, perform one of the following actions:
• Press the Stop Flow screen key.
• Type a value of 0.0 at the Flow Rate field and press Enter.
To resume flow after you have stopped the pump, type in a flow-rate value at the Flow
Rate field and press Enter.
Note: These actions do not affect the eluent composition.
172 Running Gradient Controller in Isocratic Mode
Injector.
Section 11.1.1, Using the Rheodyne 7725i
Section 9.2.4, Stopping the