Prepare The Pump; How To Deal With Solvents - Agilent Technologies Infinity II SFC Binary 1260 User Manual

3
Using the Pump
Best Practices

Prepare the pump

Purge
Use the Purge function to:
• fill the pump,
• exchange a solvent,
• remove air bubbles in tubes and pump heads.
Seal wash
Seal Wash guarantees a maximum seal life time. Use Seal Wash:
• When using buffers with elevated salt concentrations
• When using volatile solvents with non-volatile additives
Contaminated seal wash solvent
C AU T I O N
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How to deal with solvents

• Use clean bottles only.
• Exchange water-based solvents daily.
• Select solvent volume to be used up within 1 – 2 days.
• Use only HPLC-grade solvents and water filtered through 0.2 μm filters.
• Label bottles correctly with bottle content, and filling date / expiry date.
• Use solvent inlet filters.
• Reduce risk of algae growth: use brown bottles for aqueous solvents, avoid
36
Do not recycle seal wash solvent to avoid contamination.
Weekly exchange seal wash solvent.
direct sunlight.
1260 SFC Binary Pump