Page 1 1290 Infinity II High-Speed Pumps Agilent InfinityLab LC Series User Manual 1290 Infinity II High-Speed Pumps User Manual...
Page 2 Agilent Technologies, Inc. as quential damages in connection with the understood and met. furnishing, use, or performance of this...
Page 3 In This Guide… This manual covers: • Agilent 1290 Infinity II High-Speed Pump (G7120A) • Agilent 1290 Infinity II Bio High-Speed Pump (G7132A) Introduction This chapter gives an introduction to the pump and an instrument overview. Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
Page 4 Maintenance This chapter describes the maintenance of the Agilent 1290 Infinity II High-Speed Pumps. Parts and Materials for Maintenance This chapter provides information on parts for maintenance. Identifying Cables This chapter provides information on cables used with the modules. Hardware Information This chapter describes the pump in more detail on hardware and electronics.
Page 5: Table Of Contents
Contents Introduction Agilent 1290 Infinity II High-Speed Pump Agilent 1290 Infinity II Bio High-Speed Pump Pump Principle Leak and Waste Handling Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Using the Pump Magnets Turn on/off Status Indicators Best Practices Purging the Pump Recommendations for Bio-Compatible and Bio-Inert Systems Recommendations for Degassers...
Page 6 Optimizing Performance Delay Volume and Extra-Column Volume How to Configure the Optimum Delay Volume How to Achieve Higher Resolution Using Solvent Calibration Tables Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software Pump Leak Rate Test System Pressure Test Error Information What Are Error Messages General Error Messages Pump Error Messages...
Page 7 Pump Head Maintenance (Tool Free) Install the Pump Head Assembly (G7120A) Install the Pump Head Assembly (G7132A) Replace the Outlet Valve (G7120A) Replace the Outlet Valve (G7132A) Replace the Purge Valve Head Replace Parts of the Purge Valve Head Replace Parts of the High Pressure Filter Assembly (G7120A) Replace Parts of the High Pressure Filter Assembly (G7132A) Replace the Module Firmware Prepare the Pump Module for Transport...
Page 8 Manual Configuration PC and User Interface Software Setup Setup Appendix General Safety Information Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Certification for Federal Republic of Germany Agilent Technologies on Internet 1290 Infinity II High-Speed Pumps User Manual...
Page 9: Introduction
Introduction Agilent 1290 Infinity II High-Speed Pump Product Description Features Agilent 1290 Infinity II Bio High-Speed Pump Product Description Features Pump Principle Leak and Waste Handling Leak Sensor Waste Concept This chapter gives an introduction to the pump and an instrument overview. 1290 Infinity II High-Speed Pumps User Manual...
Page 10: Agilent 1290 Infinity Ii High-Speed Pump
Introduction Agilent 1290 Infinity II High-Speed Pump Agilent 1290 Infinity II High-Speed Pump Product Description The Agilent 1290 Infinity II High-Speed Pump can enhance your efficiency through high speed and chromatographic performance. A low-delay-volume mixer allows you to run fast gradients for narrow-bore applications for high laboratory efficiency.
Page 11: Features
Introduction Agilent 1290 Infinity II High-Speed Pump Features • High performance in terms of accuracy and precision for flow and composition. • High power range combining high pressure up to 1300 bar and high analytical flow rates up to 5 mL/min for high chromatographic performance. •...
Page 12: Agilent 1290 Infinity Ii Bio High-Speed Pump
Introduction Agilent 1290 Infinity II Bio High-Speed Pump Agilent 1290 Infinity II Bio High-Speed Pump Product Description The 1290 Infinity II Bio High-Speed Pump is a binary UHPLC pump consisting of biocompatible material for use in biopharma (critical quality attributes) and other applications utilizing high salt and extreme pH conditions.
Page 13: Features
Introduction Agilent 1290 Infinity II Bio High-Speed Pump Features • The biocompatible pump is resistant to corrosion from high salt concentrations and harsh cleaning procedures for increased instrument uptime. • High salt tolerance and wide pH range offers increased flexibility. •...
Page 14: Pump Principle
Introduction Pump Principle Pump Principle Agilent 1290 Infinity II High-Speed Pumps feature a dual pump head design for the generation of binary gradients. The solvent selection valve allows the choice of two solvents per pump head. However, this valve cannot be switched during a run. Each pump head is equipped with two independently actuated pistons in series.
Page 15 Introduction Pump Principle to Multisampler Solvent Seal wash sensor Seal wash pump Jet Weaver Pressure sensor Pump head B Degassing Pump head A unit Valve High-Speed Pump Figure 2 The hydraulic path 1290 Infinity II High-Speed Pumps User Manual...
Page 16: Leak And Waste Handling
Introduction Leak and Waste Handling Leak and Waste Handling The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L.
Page 17 Introduction Leak and Waste Handling Figure 3 Infinity II Leak Waste Concept (Flex Bench installation) 1290 Infinity II High-Speed Pumps User Manual...
Page 18 Introduction Leak and Waste Handling Figure 4 Infinity II Single Stack Leak Waste Concept (bench installation) 1290 Infinity II High-Speed Pumps User Manual...
Page 19: Leak Sensor
Introduction Leak and Waste Handling Figure 5 Infinity II Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container. Leak Sensor Solvent incompatibility C AU T I O N The solvent DMF (dimethylformamide) leads to corrosion of the leak sensor.
Page 20: Waste Concept
Introduction Leak and Waste Handling Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. 1290 Infinity II High-Speed Pumps User Manual...
Page 21: Site Requirements And Specifications
Site Requirements and Specifications Site Requirements Physical Specifications Physical Specifications Agilent 1290 Infinity II High-Speed Pump (G7120A) Physical Specifications Agilent 1290 Infinity II Bio High-Speed Pump (G7132A) Performance Specifications Performance Specifications Agilent 1290 Infinity II High-Speed Pump (G7120A) Performance Specifications Agilent 1290 Infinity II Bio High-Speed Pump (G7132A) This chapter provides information on environmental requirements, physical and performance specifications.
Page 22: Site Requirements
Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 25.
Page 23 Never use a power cord other than the one that Agilent shipped with this  instrument. Never use the power cords that Agilent Technologies supplies with this  instrument for any other equipment. Never use cables other than the ones supplied by Agilent Technologies ...
Page 24 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 1 on page 25) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.
Page 25: Physical Specifications
Site Requirements and Specifications Physical Specifications Physical Specifications Physical Specifications Agilent 1290 Infinity II High-Speed Pump (G7120A) Table 1 Physical Specifications G7120A Type Specification Comments Weight 21.0 kg (46.3 lbs) Dimensions 200 x 396 x 436 mm (height × width × depth) (7.9 x 15.6 x 17.2 inches) Line voltage 100 –...
Page 26: Physical Specifications Agilent 1290 Infinity Ii Bio High-Speed Pump (G7132A)
Site Requirements and Specifications Physical Specifications Physical Specifications Agilent 1290 Infinity II Bio High-Speed Pump (G7132A) Table 2 Physical Specifications G7132A Type Specification Comments Weight 22.5 kg (49.6 lbs) Dimensions 200 x 396 x 436 mm (height × width × depth) (7.9 x 15.6 x 17.2 inches) Line voltage 100 –...
Page 27: Performance Specifications
Site Requirements and Specifications Performance Specifications Performance Specifications Performance Specifications Agilent 1290 Infinity II High-Speed Pump (G7120A) Table 3 Performance Specifications G7120A Type Specification Comments Hydraulic system Two dual pistons in series pump with proprietary servo-con- trolled variable stroke design and smooth motion control for active damping.
Page 28 Site Requirements and Specifications Performance Specifications Table 3 Performance Specifications G7120A Type Specification Comments Composition precision <0.15 % RSD or 0.01 min SD, whichever is greater Composition accuracy ±0.35 % absolute Number of solvents 2 out of maximum 26 solvents Solvent selection valve Internal 4-solvent selection valve included.
Page 29 Site Requirements and Specifications Performance Specifications Table 3 Performance Specifications G7120A Type Specification Comments Safety features and mainte- Leak detection, safe leak han- nance dling, leak output signal for shutdown of the pumping sys- tem. Low voltage in major mainte- nance areas.
Page 30: Performance Specifications Agilent 1290 Infinity Ii Bio High-Speed Pump (G7132A)
Site Requirements and Specifications Performance Specifications Performance Specifications Agilent 1290 Infinity II Bio High-Speed Pump (G7132A) Table 4 Performance specifications G7132A Type Specification Comments Hydraulic system Two dual pistons in series pump with proprietary servo-con- trolled variable stroke design and smooth motion control for active damping.
Page 31 Site Requirements and Specifications Performance Specifications Table 4 Performance specifications G7132A Type Specification Comments Solvent selection valve Internal 4-solvent selection valve included. External 2 x 12 solvent valve as option, fully integrated in the pump control interface. Integrated degassing unit Number of channels: 2 Internal volume per channel: 1.5 mL...
Page 32 Site Requirements and Specifications Performance Specifications Table 4 Performance specifications G7132A Type Specification Comments GLP features Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre-defined and user settable limits and feedback messages.
Page 33: Using The Pump
Using the Pump Magnets Turn on/off Status Indicators Best Practices Daily / Weekly tasks Power up / Shut-down the pump Prepare the Pump Seal Wash How to deal with solvents Purging the Pump Recommendations for Bio-Compatible and Bio-Inert Systems Recommendations for Degassers Solvent Information Materials in Flow Path (G7120A) Materials in Flow Path (G7132A)
Page 34: Magnets
Using the Pump Magnets Magnets Magnets in doors of pumps, autosamplers, detectors, and fraction collectors. 1290 Infinity II High-Speed Pumps User Manual...
Page 35: Turn On/Off
Using the Pump Turn on/off Turn on/off This procedure exemplarily shows an arbitrary LC stack configuration. Power switch: On 1290 Infinity II High-Speed Pumps User Manual...
Page 36 Using the Pump Turn on/off Turn instrument On/Off with the control software. Power switch: Off 1290 Infinity II High-Speed Pumps User Manual...
Page 37: Status Indicators
Using the Pump Status Indicators Status Indicators This procedure exemplarily shows an arbitrary LC stack configuration. 1 The module status indicator indicates one of six possible module conditions: Status indicators 1. Idle 2. Run mode 3. Not-ready. Waiting for a specific pre-run condition to be reached or completed.
Page 38: Best Practices
Using the Pump Best Practices Best Practices Daily / Weekly tasks Daily Tasks • Replace solvents and solvent bottles for mobile phases based on water/buffer. • Replace solvents and solvent bottles for organic mobile phase latest every second day. • Check presence of seal wash solvent.
Page 39: Power Up / Shut-Down The Pump
Using the Pump Best Practices Power up / Shut-down the pump Prepare the Pump • Use fresh or different mobile phase (as required). • Purge each channel with 2.5 – 3 mL/min for 5 min. Open the manual purge valve or use the purge command, depending on the pump type. Shut Down the System •...
Page 40: Prepare The Pump
Using the Pump Best Practices Prepare the Pump The Agilent 1290 Infinity and 1290 Infinity II Pumps are equipped with automatic purge valves. This enables a variety of additional functions not available in Agilent Pumps with manual purge valves. It is possible to prepare the pump (set paramaters and start the functions Purge, Condition, or Prime) with the software.
Page 41 Using the Pump Best Practices Conditioning may be necessary: • After a long period of standby • After running out of solvent • After service or repair Filling empty solvent lines C AU T I O N Damage to the seals Use a syringe or the Purge function to fill empty solvent lines.
Page 42 Using the Pump Best Practices Figure 6 Prepare the pump (1290 Infinity). Right click on the module dashboard Select the appropriate function to start the procedure 1290 Infinity II High-Speed Pumps User Manual...
Page 43 Using the Pump Best Practices The user-optimized Prepare Pump context menu replaces the classical menu: • 1290 Infinity II Pumps Figure 7 Prepare the pump (1290 Infinity II pumps) Right click on the module dashboard Select Prepare Pump... Select the procedure and fill in adequate parameters Click Start to run the selected procedure 1290 Infinity II High-Speed Pumps User Manual...
Page 44: Seal Wash
Using the Pump Best Practices Seal Wash Seal Wash guarantees a maximum seal life time. Use Seal Wash: • When using buffers with elevated salt concentrations • When using volatile solvents with non-volatile additives Contaminated seal wash solvent C AU T I O N Do not recycle seal wash solvent to avoid contamination.
Page 45: Purging The Pump
Using the Pump Purging the Pump Purging the Pump When the solvents have been exchanged or the pumping system has been turned off for a certain time (for example, overnight) oxygen will re-diffuse into the solvent channel between the solvent reservoir, vacuum degassing unit (when available in the system) and the pump.
Page 46 Using the Pump Purging the Pump If the system has been run dry or air has diffused into the pump it might require additional steps to get rid of the air again. Following the procedure below will give the best and fastest results. 1 Change solvents to isopropanol.
Page 47: Recommendations For Bio-Compatible And Bio-Inert Systems
Using the Pump Recommendations for Bio-Compatible and Bio-Inert Systems Recommendations for Bio-Compatible and Bio-Inert Systems • Make sure all supplies (fittings, capillaries, inline filters, columns, etc.) are bio-inert / bio-compatible. • Be aware that some columns for bio-related applications have a stainless steel case and can introduce iron and other metal ions in the flow path.
Page 48 Using the Pump Recommendations for Bio-Compatible and Bio-Inert Systems Flushing Procedure  This procedure should be used when salt-containing mobile phases are used. It has to be performed regularly, at least once a week, or prior a long standby or off time, to remove salt deposits from the flow path and surfaces in contact with the solvents.
Page 49: Recommendations For Degassers
Using the Pump Recommendations for Degassers Recommendations for Degassers Liquid inside the degasser C AU T I O N In case a low boiling point solvent condensates or there is a leak, liquid can accumulate inside the degasser chambers, and this will compromise performance.
Page 50: Solvent Information
Using the Pump Solvent Information Solvent Information Observe the following recommendations on the use of solvents. • Follow recommendations for avoiding the growth of algae, see “Algae Growth in HPLC Systems” on page 64. • Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 µm filters.
Page 51: Materials In Flow Path (G7132A)
Using the Pump Solvent Information Materials in Flow Path (G7132A) Following materials are used in the flow path of this module: Table 7 Materials in flow path (G7132A) Part Materials Degasser chamber TFE/PDD copolymer, PFA (internal tubings); PEEK (inlets); FEP (tubings); ETFE (fittings) PFA (tubings), PEEK (fittings) Ultra clean tubings SST and Bio-Compatible coating...
Page 52: General Information About Solvent/Material Compatibility
Using the Pump Solvent Information General Information about Solvent/Material Compatibility Materials in the flow path are carefully selected based on Agilent’s experiences in developing highest-quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.
Page 53 Using the Pump Solvent Information Polyphenylene Sulfide (PPS) Polyphenylene sulfide has outstanding stability even at elevated temperatures. It is resistant to dilute solutions of most inorganic acids, but it can be attacked by some organic compounds and oxidizing reagents. Nonoxidizing inorganic acids, such as sulfuric acid and phosphoric acid, have little effect on polyphenylene sulfide, but at high concentrations and temperatures, they can still cause material damage.
Page 54 Using the Pump Solvent Information Polyimide Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent. Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g.
Page 55 Using the Pump Solvent Information Stainless Steel (SST) Stainless steel is inert against many common solvents. It is stable in the presence of acids and bases in a pH range of 1 to 12.5. It can be corroded by acids below pH 2.3. It can also corrode in following solvents: •...
Page 56 Using the Pump Solvent Information Diamond-Like Carbon (DLC) Diamond-Like Carbon is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Fused silica and Quartz (SiO Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell windows.
Page 57 Using the Pump Solvent Information Fluorinated polymers (PTFE, PFA, FEP, FFKM, PVDF) Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy), and FEP (fluorinated ethylene propylene) are inert to almost all common acids, bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to most chemicals.
Page 58: Solvent Recommendation For Agilent 1290 Infinity And 1290 Infinity Ii And 1260 Infinity Ii Flexible Pumps
Using the Pump Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps While the Agilent 1290 Infinity, 1290 Infinity II, and 1260 Infinity II Flexible Pumps guarantee a high performance with a wide variety of solvents, other solvents may cause harm to the pump or to the (U)HPLC system.
Page 59: Normal Phase Applications
Using the Pump Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Normal Phase Applications Valves for Normal Phase Applications (G7120A) Current passive inlet valves and outlet ball valves used with Agilent Infinity and Infinity II pumps do not work well while running nonpolar solvents for normal phase applications (for example, hexane and heptane).
Page 60 Using the Pump Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Seals for Normal Phase Applications For running normal phase applications on Agilent Infinity and Infinity II pumps, yellow PE seals are required as piston seals. By default, 1290 Infinity, 1290 Infinity II, and 1260 Infinity II Flexible Pumps use ceramic pistons and yellow PE seals as piston seals.
Page 61: Solvent Handling
Using the Pump Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Solvent Handling Handling of Normal Phase Solvents Observe the following recommendations when using normal phase solvents: • Always use fresh, filtered solvents. Exchange solvents every second day. •...
Page 62 Using the Pump Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps • Use a minimum flow rate of 5 µL/min or 1 % composition per solvent channel (whatever is greater) to avoid cross-flow. Cross-flow can be caused by micro leaks in pump heads and can result in buffer precipitation in pump heads, channel blocking, or reduced pump head life time through wear of seals and pistons.
Page 63 Using the Pump Solvent Recommendation for Agilent 1290 Infinity and 1290 Infinity II and 1260 Infinity II Flexible Pumps Handling of Acids Acids can corrode stainless steel and other materials in the flow path of LC systems. For stainless steel, the minimum pH is 2.3 for corrosive acids and pH 1 for non-corrosive acids.
Page 64: Algae Growth In Hplc Systems
Using the Pump Algae Growth in HPLC Systems Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems. Algae grow in aqueous media, preferably in a pH range of 4-8. Their growth is accelerated by buffers, for example phosphate or acetate.
Page 65: Setting Up The Pump With The Instrument Control Interface
Using the Pump Setting up the Pump with the Instrument Control Interface Setting up the Pump with the Instrument Control Interface Overview Parameters described in following sections are offered by the instrument control interface and can usually be accessed through Agilent instrument control software.
Page 66 Using the Pump Setting up the Pump with the Instrument Control Interface Options: • Pressure Unit: Select the pressure units from the drop-down list (bar, psi, or MPa). • Installed mixer: The installed mixer is detected during auto configuration. For manual configuration, click the down-arrow and select the installed mixer from the list.
Page 67: The Pump User Interface (Dashboard Panel)
Using the Pump Setting up the Pump with the Instrument Control Interface The Pump User Interface (Dashboard Panel) Module Graphic The items in the pump graphic have the following meaning and function: Indicates that an External Contacts board is installed. The level of solvent in the bottle is denoted by the green area;...
Page 68 Using the Pump Setting up the Pump with the Instrument Control Interface Instrument Signals The following pump signals are displayed: Flow The current solvent flow rate (in mL/min). Pressure The current pump ressure (in bar, psi or MPa, see “Instrument Configuration”...
Page 69 Using the Pump Setting up the Pump with the Instrument Control Interface Prepare Pump Dialog Table 9 Prepare Pump dialog Parameter Limits Description Purge Time: 0 – 100.00 min Setup and activation of Purge parameters. The automatic purge valve can in steps of 0.01.
Page 70: Control Settings
Using the Pump Setting up the Pump with the Instrument Control Interface Control Settings The pump control parameters are in two sections: • Pump • Automatic Turn On Table 10 Pump control parameters Parameter Description Pump Enables you to switch the pump On, Off or to a Standby condition. In the Standby condition, the pump motor is still active, and when the pump is switched on again, it does not need to be re-initialized.
Page 71: Method Parameter Settings
Using the Pump Setting up the Pump with the Instrument Control Interface Method Parameter Settings The High-Speed Pump method setup parameters are in eight sections: • Flow • Solvents A and B • Stoptime • Posttime • Pressure Limits • Timetable •...
Page 72 Using the Pump Setting up the Pump with the Instrument Control Interface Table 11 Method parameters Parameter Limits Description Pressure Limits Max: 1300 bar Sets the maximum and minimum pressure limits for the pump. (18850 psi) for flow • Max is the maximum pressure limit at which the pump will switch itself rates up to 2 mL/min.
Page 73 Using the Pump Setting up the Pump with the Instrument Control Interface Advanced Settings The High-Speed Pump advanced method setup parameters are in three sections: • Minimum Stroke • Compressibility • Maximum Flow Gradient Table 12 Advanced method parameters Parameter Limits Description Minimum Stroke...
Page 74 Using the Pump Setting up the Pump with the Instrument Control Interface Timetable Settings Use the Timetable to program changes in the pump parameters during the analysis by entering a time in the Time field and appropriate values in the following fields of the timetable.
Page 75: Agilent Local Control Modules
Using the Pump Agilent Local Control Modules Agilent Local Control Modules Agilent InfinityLab Companion G7108AA The Agilent InfinityLab Companion gives you complete control, system monitoring, signal plotting, and diagnostic capabilities for a wide range of LC system modules. The instrument control solution is available as full package including all hardware and accessories, but can also be used on your own mobile devices like tablets, mobile phones and other electronic equipment.
Page 76: Preparing The Pump
Preparing the Pump Capillary Coding Guide Syntax for Capillary Description At-a-Glance Color-Coding Keys Installing Capillaries This chapter provides information on how to set up the module for an analysis and explains the basic settings. 1290 Infinity II High-Speed Pumps User Manual...
Page 77: Capillary Coding Guide
Preparing the Pump Capillary Coding Guide Capillary Coding Guide Syntax for Capillary Description The tables below are your guide to identifying the proper specifications for your capillary. On all capillaries, dimensions are noted in id (mm), length (mm) and, where applicable, volume (µL). When you receive your capillary, these abbreviations are printed on the packaging.
Page 78 Preparing the Pump Capillary Coding Guide Table 13 Capillary coding guide Type Material Fitting left/fitting right The type gives some The material indicates The fitting left/right indication on the pri- which raw material is indicate which fitting mary function, like a used.
Page 79 Preparing the Pump Capillary Coding Guide At-a-Glance Color-Coding Keys The color of your capillary will help you quickly identify the capillary id. Table 14 Color-coding key for Agilent capillary tubing Internal diameter in Color code 0.015 Orange 0.025 Yellow 0.05 Beige 0.075 Black...
Page 80: Installing Capillaries
Preparing the Pump Installing Capillaries Installing Capillaries Capillaries and connections depend on which system is installed. Table 15 Capillary connections for 1260 Infinity II systems From Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump Capillary ST 0.17 mm x 500 mm SI/SI (5500-1246) Pump Sampler Capillary, ST, 0.17 mm x 900 mm SI/SX (5500-1217)
Page 81 Preparing the Pump Installing Capillaries Table 16 Capillary connections for 1290 Infinity II systems From Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump Capillary ST 0.17 mm x 400 mm SI/SI (5500-1245) Pump Sampler Capillary, ST, 0.17 mm x 900 mm SI/SX (5500-1217) Pump Vialsampler with ICC Capillary ST, 0.12 mm x 500 mm SL/S (5500-1157)
Page 82 Preparing the Pump Installing Capillaries Table 18 Capillary connections for 1290 Infinity II Bio LC From Bottle Head Assembly (G7120-60007) Solvent Bottle Infinity II Pump Capillary MP35N 0.17 mm x 500 mm, SI/SI (5500-1419) Pump Multisampler Capillary MP35N 0.12 mm x 500 mm SI/SI (5500-1279) Multisampler Quick Connect Capillary MP35N 0.12 mm x 105 mm MCT Heat Exchanger...
Page 83 Preparing the Pump Installing Capillaries For correct installation of capillary connections it's important to choose the correct fittings, see “Syntax for Capillary Description” on page 77. Select a nut that is long enough for the fitting you'll be Slide the nut over the end of the tubing or capillary. using.
Page 84 Preparing the Pump Installing Capillaries Loosen the nut and verify that the ferrule is correctly positioned on the tubing or capillary. The first time that the swagelock fitting is used on a column or an injection valve, NOTE the position of the ferrule is permanently set. If changing from a column or an injection valve to another, the fitting may leak or decrease the quality of the separation by contributing to band broadening.
Page 85: Optimizing Performance
Optimizing Performance Delay Volume and Extra-Column Volume Delay Volume How to Configure the Optimum Delay Volume How to Achieve Higher Resolution Using Solvent Calibration Tables How it Works How to Import the Latest Solvent Calibration Tables Recommendations for Setting up Methods Using Salt-Containing Eluents This chapter gives hints on how to optimize performance or use additional devices.
Page 86: Delay Volume And Extra-Column Volume
Optimizing Performance Delay Volume and Extra-Column Volume Delay Volume and Extra-Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the front of the column. The extra-column volume is defined as the volume between the injection point and the detection point, excluding the volume in the column.
Page 87: How To Configure The Optimum Delay Volume
Optimizing Performance How to Configure the Optimum Delay Volume How to Configure the Optimum Delay Volume The physical delay volume of the pump depends primarily on the use of the Jet Weaver mixer. For UV detection, the Jet Weaver should always be used but for mass spectrometric detection the user can decide to bypass the Jet Weaver to reduce the delay volume.
Page 88: How To Achieve Higher Resolution
Optimizing Performance How to Achieve Higher Resolution How to Achieve Higher Resolution Increased resolution in a separation will improve the qualitative and quantitative data analysis, allow more peaks to be separated or offer further scope for speeding up the separation. This section explains how resolution can be increased by examining the following points: •...
Page 89 Optimizing Performance How to Achieve Higher Resolution proportionally with the length of the column. Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1.4. What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure.
Page 90 Optimizing Performance How to Achieve Higher Resolution This shows that k and hence resolution can be increased by having shallower gradients (2 to 5 %/min change is a guideline), higher flow rates and smaller volume columns. This equation also shows how to speed up an existing gradient –...
Page 91: Using Solvent Calibration Tables
Optimizing Performance Using Solvent Calibration Tables Using Solvent Calibration Tables Agilent Solvent Calibration Tables provide an algorithm for the pump to automatically determine the correct compressibility associated with the current system pressure. Solvent definition tables for most common solvents are now available for download.
Page 92: How To Import The Latest Solvent Calibration Tables
Optimizing Performance Using Solvent Calibration Tables How to Import the Latest Solvent Calibration Tables It might be necessary to import new Agilent Solvent Calibration tables that were not previously available with the system. This will then provide the most accurate algorithm in determining the compressibility of the mobile phase.
Page 93 Optimizing Performance Using Solvent Calibration Tables 2 Click on the module that needs the latest solvent calibration table (ie G7120A). Then choose configure, launching the additional configuration of the pump module. 3 In the additional configuration of the pump module, choose Configure Solvent Type Catalogs…...
Page 94 Optimizing Performance Using Solvent Calibration Tables 4 In the Solvent Type Catalogs, press the Import button. 5 Navigate to the location of the downloaded Agilent Solvent Calibration Table and click the Open button. Keep in mind multiple solvent calibration tables may be imported at the same time.
Page 95: Recommendations For Setting Up Methods Using Salt-Containing Eluents
Optimizing Performance Using Solvent Calibration Tables Recommendations for Setting up Methods Using Salt-Containing Eluents With the 1290 Infinity II Bio LC System Agilent offers a dedicated instrument for running applications that are typical for the (Bio)pharma market, such as IEX (Ion Exchange Chromatography)or HIC (Hydrophobic Interaction Chromatography), and applications in Academia, Government and Applied Markets, using concentrated salt solutions as eluents.
Page 96: Troubleshooting And Diagnostics
Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software Pump Leak Rate Test Troubleshooting the Pump Leak Rate Test System Pressure Test Overview about the troubleshooting and diagnostic features. 1290 Infinity II High-Speed Pumps User Manual...
Page 97: User Interfaces
Troubleshooting and Diagnostics User Interfaces User Interfaces • Depending on the user interface, the available tests and the screens/reports may vary. • The preferred tool for troubleshooting and diagnostics should be Agilent Lab Advisor Software, see “Agilent Lab Advisor Software” on page 98.
Page 98: Agilent Lab Advisor Software
Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument. The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines.
Page 99: Pump Leak Rate Test
Troubleshooting and Diagnostics Pump Leak Rate Test Pump Leak Rate Test The Pump Leak Rate Test is a diagnostic test to check the integrity and tightness of the pump components. The test is started from the Services & Diagnostics section in the Agilent Lab Advisor Software. The test is first evaluating the tightness from the outlet valve downstream to the purge valve.
Page 100 Troubleshooting and Diagnostics Pump Leak Rate Test Evaluation: The result as well as the applied limits are displayed after the automatic evaluation. The limits are: • The allowed leak limit for the secondary piston is ≤3 µL/min • The allowed leak limit for the primary piston is ≤30 µL/min A report can be displayed, saved or printed by opening it with the Print Result button at the lower right of the screen.
Page 101 Troubleshooting and Diagnostics Pump Leak Rate Test Figure 10 Dynamic (primary) Leak Test 1290 Infinity II High-Speed Pumps User Manual...
Page 102: Troubleshooting The Pump Leak Rate Test
Troubleshooting and Diagnostics Pump Leak Rate Test Troubleshooting the Pump Leak Rate Test Secondary Leak > 3 µL/min • Leak between the OBV and automatic purge valve • Check for visible leaks on fittings and connectors • Connector not fixed / tight enough •...
Page 103 Troubleshooting and Diagnostics Pump Leak Rate Test • Outlet valve not properly assembled • Retighten the outlet valve • Check the position of the gold seal • Leaky piston seals and/or position-dependent leaks on the piston • Remove the SW tubes from the support ring and check for leaks •...
Page 104: System Pressure Test
Troubleshooting and Diagnostics System Pressure Test System Pressure Test The System Pressure Test is performed to evaluate the leak tightness of the system up to the point where the system is capped off. The test is started from the Services & Diagnostics section in the Agilent Lab Advisor Software or in the Local Controller.
Page 105 Troubleshooting and Diagnostics System Pressure Test Figure 11 System Pressure Test 1290 Infinity II High-Speed Pumps User Manual...
Page 106: Error Information
Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Short Leak Sensor Open Compensation Sensor Open Compensation Sensor Short Fan Failed Leak Open Cover Cover Violation Pump Error Messages Pressure of binary pump above upper limit Pressure below lower limit Target pressure not reached for binary pump degasser Degasser's pressure limit violation...
Page 107 Error Information System Pressure Test Current of primary pump drive too high Current of secondary pump drive too high Unknown purge valve type Pump drive encoder error Pump drive error Pump drive stroke blocked Pump drive stop not found Pressure sensor calibration wrong or missing Seal wash pump was missing when tried to turn on Invalid degasser pressure signal This chapter describes the meaning of error messages, and provides information...
Page 108: What Are Error Messages
Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).
Page 109: General Error Messages
Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout Error ID: 0062 The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and source The analysis was completed successfully, of a not-ready condition.
Page 110: Shutdown
Error Information General Error Messages Shutdown Error ID: 0063 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.
Page 111: Remote Timeout
Error Information General Error Messages Remote Timeout Error ID: 0070 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.
Page 112: Leak Sensor Short
Error Information General Error Messages Leak Sensor Short Error ID: 0082 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 113: Leak Sensor Open
Error Information General Error Messages Leak Sensor Open Error ID: 0083 The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 114: Compensation Sensor Short
Error Information General Error Messages Compensation Sensor Short Error ID: 0080 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes.
Page 115: Leak
Error Information General Error Messages Leak Error ID: 0064 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent.
Page 116: Cover Violation
Error Information General Error Messages Cover Violation Error ID: 7461 The top foam has been removed. The sensor on the main board detects when the top foam is in place. If the foam is removed while the lamps are on (or if an attempt is made to switch on for example the lamps with the foam removed), the lamps are switched off, and the error message is generated.
Page 117: Pump Error Messages
Error Information Pump Error Messages Pump Error Messages These errors are pump specific. Pressure of binary pump above upper limit Error ID: 22014 The pressure has exceeded the upper pressure limit. • Parameter: Measured pressure Probable cause Suggested actions • Check for blockages in the LC system, e.g.
Page 118: Pressure Below Lower Limit
Error Information Pump Error Messages Pressure below lower limit Error ID: 22015 The pressure has dropped below the lower limit. • Parameter: None Probable cause Suggested actions Check for leaks. Leak Check bottle filling. Bottle empty Check solvent. Wrong solvent (viscosity) Check flow rate and lower pressure limit.
Page 119: Degasser's Pressure Limit Violation
Error Information Pump Error Messages Degasser's pressure limit violation Error ID: 22032 Pressure too far above the limit. Probable cause Suggested actions Please contact your Agilent service representa- Leak in degasser chamber or degasser tub- tive. ing. Please contact your Agilent service representa- Defect vacuum pump.
Page 120: Waste Counter Limit Exceeded
Error Information Pump Error Messages Waste counter limit exceeded Error ID: 22056 The counter for the waste volume has exceeded the limit, which has been set in the user interface. • Parameter: None Probable cause Suggested actions Empty waste container. The waste container is full.
Page 121: Binary Pump Shutdown During Analysis
Error Information Pump Error Messages Binary pump shutdown during analysis Error ID: 22065 The binary pump has been shut down by the control software or control module during an analysis. • Parameter: 0 for off, 1 for standby. Probable cause Suggested actions Restart pump.
Page 122: Writing The Pump Encoder Tag Failed
Error Information Pump Error Messages Writing the pump encoder tag failed Error ID: 22405 Writing the pump encoder tag has failed. • Parameter: 1 – 4 referring to pump drive Probable cause Suggested actions Please contact your Agilent service representa- Defect connection between encoder and tive.
Page 123: Drive Current Too Low
Error Information Pump Error Messages Drive current too low Error ID: 22407 The current consumption of the pump drive is too low. • Parameter: 1 – 4 referring to pump drive Probable cause Suggested actions Please contact your Agilent service representa- Drive motor defect.
Page 124: Drive Timeout
Error Information Pump Error Messages Drive timeout Error ID: 22410 Drive is blocked mechanically, fails during initialization. • Parameter: 1 – 4 referring to pump drive Probable cause Suggested actions Check for blockage of e.g. outlet valve filter frit, Blockage of system before pressure sensor. purge valve, heat exchanger.
Page 125: Deliver Underrun
Error Information Pump Error Messages Deliver underrun Error ID: 22413 Internal error. • Parameter: None Probable cause Suggested actions Please contact your Agilent service representa- Internal error. tive. Defect connection between main board and pump drive encoder Error ID: 22414 Defect connection between main board and pump drive encoder.
Page 126: Purge Valve Failed
Error Information Pump Error Messages Purge valve failed Error ID: 22417 Lost steps of the purge valve encoder. • Parameter: None Probable cause Suggested actions • Check installation of purge valve head. Purge valve drive mechanically blocked or defect. • Please contact your Agilent service represen- tative.
Page 127: Drive Position Limit
Error Information Pump Error Messages Drive position limit Error ID: 22425 Internal error. • Parameter: 1 – 4 referring to pump drive Probable cause Suggested actions Please contact your Agilent service representa- Internal error. tive. Insufficient power of drive encoder LED Error ID: 22426 Insufficient power of drive encoder LED.
Page 128: Writing The Purge Valve Tag Failed
Error Information Pump Error Messages Writing the purge valve tag failed Error ID: 22431 Writing the purge valve tag failed. • Parameter: None Probable cause Suggested actions Replace purge valve head. Purge valve head tag defect. Please contact your Agilent service representa- Purge valve tag reader is defect.
Page 129: Current Of Secondary Pump Drive Too High
Error Information Pump Error Messages Current of secondary pump drive too high Error ID: 22434 The current of the secondary pump drive is too high. • Parameter: 2 or 3 referring to pump drive Probable cause Suggested actions • Check for blockages in the flow path. Blockage of flow path between secondary pump head and pressure sensor, e.g.
Page 130: Pump Drive Error
Error Information Pump Error Messages Pump drive error Error ID: 22438, 22439 The pump drive failed during calibration. • Parameter: 1 – 4 referring to pump drive Probable cause Suggested actions Please contact your Agilent service representa- Pump drive motor defect or mechanically tive.
Page 131: Pump Drive Stop Not Found
Error Information Pump Error Messages Pump drive stop not found Error ID: 22442 The maximum stroke is too long. • Parameter: 1 – 4 referring to pump drive Probable cause Suggested actions Please contact your Agilent service representa- Wiper shifted tive.
Page 132: Seal Wash Pump Was Missing When Tried To Turn On
Error Information Pump Error Messages Seal wash pump was missing when tried to turn on Error ID: 22499 The seal wash pump has not been detected (while being configured or detected before) Probable cause Suggested actions Defect cable connection to seal wash pump. Check cable connection. Please contact your Agilent service representa- Defect seal wash pump motor.
Page 133: Maintenance
Maintenance Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Install Fittings and Capillaries Remove and Install Doors Replace the Shutoff Valve Panel Replace the Pressure Sensor Replace the Solvent Selection Valve (SSV) Change Configuration or Replace the Jet Weaver Replace the Seal Wash Pump Replace the Inlet Valve (G7120A) Replace the Inlet Valve (G7132A)
Page 134 Maintenance Pump Error Messages Replace Parts of the High Pressure Filter Assembly (G7132A) Replace the Module Firmware Prepare the Pump Module for Transport This chapter describes the maintenance of the Agilent 1290 Infinity II High-Speed Pumps. 1290 Infinity II High-Speed Pumps User Manual...
Page 135: Introduction To Maintenance
Maintenance Introduction to Maintenance Introduction to Maintenance Figure 12 on page 135 and Figure 13 on page 136 show the main user accessible assemblies of the Agilent 1290 Infinity II High-Speed Pump (G7120A) and Agilent 1290 Infinity II Bio High-Speed Pump (G7132A). These parts can be accessed from the front (simple repairs) and don’t require to remove the pump from the system stack.
Page 136 Maintenance Introduction to Maintenance Jet Weaver Solvent Selection Valve (SSV) Seal Wash Pump Seal Wash Sensor Leak Tube Left Pump Head Assembly Purge Valve Right Pump Head Assembly Pressure Sensor Degasser Figure 13 Maintenance parts (G7132A) Figure 14 on page 137 shows the flow connections between these main assemblies.
Page 137 Maintenance Introduction to Maintenance to Multisampler Solvent Seal wash sensor Seal wash pump Jet Weaver Pressure sensor Pump head B Degassing Pump head A unit Valve High-Speed Pump Figure 14 The hydraulic path Recommended Interval for Preventive Maintenance The recommended interval for preventive maintenance is: •...
Page 138: Warnings And Cautions
Maintenance Warnings and Cautions Warnings and Cautions Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety ...
Page 139 Maintenance Warnings and Cautions Heavy weight WAR N IN G The module is heavy. Carry the module at least with 2 people.  Avoid back strain or injury by following all precautions for lifting heavy  objects. Ensure that the load is as close to your body as possible. ...
Page 140: Overview Of Maintenance
Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the module that can be carried out without opening the main cover. 1290 Infinity II High-Speed Pumps User Manual...
Page 141: Cleaning The Module
Maintenance Cleaning the Module Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Avoid using organic solvents for cleaning purposes. They can cause damage to plastic parts. Liquid dripping into the electronic compartment of your module can cause WAR N IN G shock hazard and damage the module...
Page 142: Install Fittings And Capillaries
Maintenance Install Fittings and Capillaries Install Fittings and Capillaries Solvent can spray under high pressure. WAR N IN G Observe appropriate safety procedures (for example, goggles, safety  gloves and protective clothing), when opening flow path. Deformation of fittings and seals C AU T I O N Liquid drops under high pressure act like solid parts.
Page 143: Remove And Install Doors
Maintenance Remove and Install Doors Remove and Install Doors Parts required Description 5067-5767 Door assy 200 left IF II 5067-5768 Door assy 200 right IF II The figures shown in this procedure exemplarily show the Infinity II Multisampler NOTE module. The principle of how to remove and/or install doors works in the same way for all Infinity II modules.
Page 144 Maintenance Remove and Install Doors Press the release buttons and pull the front door out. For the Installation of the front door. Insert the hinges into their guides and move the door in until the release buttons click into their final position. 1290 Infinity II High-Speed Pumps User Manual...
Page 145: Replace The Shutoff Valve Panel
Maintenance Replace the Shutoff Valve Panel Replace the Shutoff Valve Panel When If a shutoff valve is damaged or the panel needs to be removed for other repair procedures. Parts required Description 5067-4124 Shutoff valve G7120-40004 Valve Holder Left G4220-60035 Tubing kit 140 mm, 2/pk SSV to shutoff valve or degassing unit Preparations...
Page 146: Replace The Pressure Sensor
Maintenance Replace the Pressure Sensor Replace the Pressure Sensor When No or invalid pressure signal Tools required Description 8710-2412 Hex key 2.5 mm, 15 cm long, straight handle 8710-0510 Open-end wrench 1/4 — 5/16 inch Screwdriver Parts required Description 5067-6791 Pressure sensor 1300 bar 5067-7010 Pressure sensor Bio-Compatible 1300 bar...
Page 147 Maintenance Replace the Pressure Sensor Carefully pull out the pressure sensor for about 2 cm. Connect the new pressure sensor to the pressure sen- Then unscrew the cable from the pressure sensor. sor connector. Fix the pressure sensor to the instrument chassis. Connect the capillaries from the valve to the pressure sensor: connect port 3 to the pressure sensor inlet and port 2 to the outlet.
Page 148: Replace The Solvent Selection Valve (Ssv)
Maintenance Replace the Solvent Selection Valve (SSV) Replace the Solvent Selection Valve (SSV) When In case of problems with the solvent selection valve Parts required Description G7120-60029 SSV Valve Assembly G4220-60035 Tubing kit 140 mm, 2/pk SSV to shutoff valve or degassing unit Close the shutoff valves.
Page 149 Maintenance Replace the Solvent Selection Valve (SSV) Install a new SSV by inserting the connector and clip- ping the SSV panel to the module top panel. Then rein- stall all tubing connections, and open the shutoff valves. 1290 Infinity II High-Speed Pumps User Manual...
Page 150: Change Configuration Or Replace The Jet Weaver
Maintenance Change Configuration or Replace the Jet Weaver Change Configuration or Replace the Jet Weaver When For optimizing the pump configuration to mixing performance or low delay volumes/fast gradients, see chapter Optimizing Performance. Tools required Description 8710-0510 ¼ inch wrench 3 mm hex key Parts required Description...
Page 151 Maintenance Change Configuration or Replace the Jet Weaver Remove capillary connections from the Jet Weaver. Remove the hex screws that fix the Jet Weaver to the pump housing. NOTE The standard Jet Weavers (Jet Weaver 35 μL/ 100 μL (G7120-68135) and Jet Weaver 35 µL/ 100 µL Bio-Compati- ble (G7132-60135)) have a front and a rear side with differ- ent internal volumes (35 / 100 µL) that are optimized for a low delay volume or best mixing performance.
Page 152 Maintenance Change Configuration or Replace the Jet Weaver Fix the Jet Weaver with the screws. Reinstall the capillary connections. The inlet at the left side of the Jet Weaver is connected to the central port of the pump valve by a capillary (length 300 mm, 0.17 mm i.d.).
Page 153: Replace The Seal Wash Pump
Maintenance Replace the Seal Wash Pump Replace the Seal Wash Pump When In case of seal wash pump wear Parts required Description 5065-4445 Peristaltic pump with PharMed tubing 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m Preparations Remove the flow connections from and to the seal wash pump Press the clips.
Page 154: Replace The Inlet Valve (G7120A)
Maintenance Replace the Inlet Valve (G7120A) Replace the Inlet Valve (G7120A) When If Inlet valve is defective. Tools required Description Wrench, 14 mm 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench Parts required Description G4220-60022 Inlet valve (primary pump head) For best performance, lifetime and to avoid leaks, use a torque wrench set to NOTE...
Page 155 Maintenance Replace the Inlet Valve (G7120A) Attach the inlet tubing to the inlet valve. Open the shutoff valves and purge the system to remove air. 1290 Infinity II High-Speed Pumps User Manual...
Page 156: Replace The Inlet Valve (G7132A)
Maintenance Replace the Inlet Valve (G7132A) Replace the Inlet Valve (G7132A) When If Inlet valve is defective. Tools required Description Wrench, 14 mm 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench Parts required Description G7131-60021 Inlet Valve Bio-Compatible (primary pump head) For best performance, lifetime and to avoid leaks, use a torque wrench set to NOTE...
Page 157 Maintenance Replace the Inlet Valve (G7132A) Attach the inlet tubing at the inlet valve. Open the shut off valves and purge the system to remove air. 1290 Infinity II High-Speed Pumps User Manual...
Page 158: Release A Stuck Inlet Valve
Maintenance Release a Stuck Inlet Valve Release a Stuck Inlet Valve When If inlet valve is stuck, or if pump is not generating pressure after being turned off for an extended period of time. Before the system is turned off for an extended period of time, it should be NOTE flushed with at least 10 % isopropanol to prevent inlet valves from getting stuck.
Page 159 Maintenance Release a Stuck Inlet Valve Unscrew tubing at degassing unit and attach the Push solvent with syringe until it comes out at the top of syringe to it. the High Pressure Filter Assembly. Detach the syringe and reconnect the tubing into the Reinstall the capillary connection to the High Pressure degassing unit.
Page 160: Remove The Pump Head Assembly (G7120A)
Maintenance Remove the Pump Head Assembly (G7120A) Remove the Pump Head Assembly (G7120A) Tools required Description G7120-68708 HPLC System Tool Kit-Infinity-II This procedure describes the replacement of the left pump head assembly NOTE (channel A). Similarly, the right pump head assembly (channel B) can be replaced.
Page 161 Maintenance Remove the Pump Head Assembly (G7120A) Remove the capillary connection at the top of the sec- Remove the flow connection between the degassing ondary pump head to the pump valve. unit and the primary pump head inlet. Loosen the inlet valve. Keep the inlet valve installed to Counter the lock screw of the heat exchanger capillary the pump head assembly.
Page 162 Maintenance Remove the Pump Head Assembly (G7120A) 11 Remove the complete pump head assembly by holding 12 Remove the seal wash tubing interconnecting the two both heads and pulling it to the front. pump heads. 1290 Infinity II High-Speed Pumps User Manual...
Page 163: Remove The Pump Head Assembly (G7132A)
Maintenance Remove the Pump Head Assembly (G7132A) Remove the Pump Head Assembly (G7132A) Tools required Description G7120-68708 HPLC System Tool Kit-Infinity-II This procedure describes the replacement of the left pump head assembly NOTE (channel A). Similarly, the right pump head assembly (channel B) can be replaced.
Page 164 Maintenance Remove the Pump Head Assembly (G7132A) Remove the capillary connection at the top of the sec- Remove the flow connection between the degassing ondary pump head to the pump valve. unit and the primary pump head inlet. Loosen the inlet valve. Keep the inlet valve installed to Counter the lock screw of the heat exchanger capillary the pump head assembly.
Page 165 Maintenance Remove the Pump Head Assembly (G7132A) 11 Remove the complete pump head assembly by holding 12 Remove the seal wash tubing interconnecting the two both heads and pulling it to the front. pump heads. 1290 Infinity II High-Speed Pumps User Manual...
Page 166: Pump Head Maintenance (Tool Free)
Maintenance Pump Head Maintenance (Tool Free) Pump Head Maintenance (Tool Free) Infinity II Flexible Pumps (G7104A/C) and 1290 Infinity II High-Speed Pumps (G7120A and G7132A) are equipped with Long Life Pump Heads. Long Life Pump Heads offer a significantly increased lifetime of pistons and seals compared to other pump heads.
Page 167: Disassemble Longlife Pump Heads (G7120A)
Maintenance Pump Head Maintenance (Tool Free) Disassemble LongLife Pump Heads (G7120A) This procedure shows how to open the pump head assembly, exchange seals, and clean pistons. Exchanging seals and cleaning pistons is exemplarily shown for the primary pump head, but works in the same way for the secondary pump head. Tools required Description G7120-68708...
Page 168 Maintenance Pump Head Maintenance (Tool Free) Turn the pump head assembly upside down. Remove the link plate by gently pulling it off the pump head assembly. The two pump chambers are now isolated. Remove the inlet valve and the outlet valve from the pri- Binary/High-Speed Pumps only: Remove the high pres- mary pump head.
Page 169 Maintenance Pump Head Maintenance (Tool Free) Open the pump heads and remove the piston housings 10 Remove the piston by pressing it out of the seal holder from the pump chambers. with a finger. 11 Remove the seal holder from the spring housing. 12 Screw the pin of the Seal Handling Device into the pis- ton seal.
Page 170 Maintenance Pump Head Maintenance (Tool Free) 15 Screw the pin of the Seal Handling Device into the wash 16 Pull out the Seal Handling Device with the wash seal in a seal. straight movement with only gentle force. NOTE The seal holder has two different sides. The black backup ring is supporting the piston seal and must not be removed.
Page 171: Disassemble Longlife Pump Heads (G7132A)
Maintenance Pump Head Maintenance (Tool Free) Disassemble LongLife Pump Heads (G7132A) This procedure shows how to open the pump head assembly, exchange seals, and clean pistons. Exchanging seals and cleaning pistons is exemplarily shown for the primary pump head, but works in the same way for the secondary pump head. Tools required Description G7120-68708...
Page 172 Maintenance Pump Head Maintenance (Tool Free) Turn the pump head assembly upside down. Remove the link plate by gently pulling it off the pump head assembly. The two pump chambers are now isolated. Remove the inlet valve and the outlet valve from the pri- Binary/High-Speed Pumps only: Remove the high-pres- mary pump head.
Page 173 Maintenance Pump Head Maintenance (Tool Free) Open the pump heads and remove the piston housings 10 Remove the piston by pressing it out of the seal holder from the pump chambers. with a finger. 11 Remove the seal holder from the spring housing. 12 Screw the pin of the Seal Handling Device into the pis- ton seal.
Page 174 Maintenance Pump Head Maintenance (Tool Free) 15 Screw the pin of the Seal Handling Device into the wash 16 Pull out the Seal Handling Device with the wash seal in a seal. straight movement with only gentle force. NOTE The seal holder has two different sides. The black backup ring is supporting the piston seal and must not be removed.
Page 175: Replace The Heat Exchanger (G7120A)
Maintenance Pump Head Maintenance (Tool Free) Replace the Heat Exchanger (G7120A) Tools required Description Wrench, 19 mm 5023-2501 Screwdriver Torx-T10 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench G4220-20013 4 mm hex bit G4220-20015 Adapter ¼ in square to hex G4220-20041 Bit Torx 10x25 mm Parts required...
Page 176 Maintenance Pump Head Maintenance (Tool Free) Remove the screw at the bottom of the pump head. Do Lift out the heat exchanger. not drop the golden spacer fitting. Screw If removed, first insert the spacer fitting. Then insert the Use the 19 mm screw for fixing the front plate. new heat exchanger to the opening in the pump head and lift it over the pins.
Page 177: Replace The Heat Exchanger (G7132A)
Maintenance Pump Head Maintenance (Tool Free) Replace the Heat Exchanger (G7132A) For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules. Tools required Description Wrench, 19 mm...
Page 178 Maintenance Pump Head Maintenance (Tool Free) Remove the screw at the bottom of the pump head. Lift out the heat exchanger. Screw Insert the new heat exchanger into the opening of the Use the 19 mm screw for fixing the front plate. pump head and lift it over the pins.
Page 179: Assemble Longlife Pump Heads (G7120A)
Maintenance Pump Head Maintenance (Tool Free) Assemble LongLife Pump Heads (G7120A) This procedure shows how to exchange seals, and reassemble the pump head assembly. Exchanging seals is exemplarily shown for the primary pump head, but works in the same way for the secondary pump head. Tools required Description G7120-68708...
Page 180 Maintenance Pump Head Maintenance (Tool Free) Take care that the Seal Handling Device is seating flush Repeat for the other pump chamber. and press the seal into the pump chamber. Place the seal holder onto the pump chamber. Place the wash seal onto the designated nose of the Seal Handling Device.
Page 181 Maintenance Pump Head Maintenance (Tool Free) Take care that the Seal Handling Device is seating flush Repeat for the other seal holder. and press the wash seal into the seal holder. NOTE The Seal Handling Device has a cavity to fit over the pins of the seal wash tubings.
Page 182 Maintenance Pump Head Maintenance (Tool Free) 11 Place the seal holder onto the spring housing. 12 Place the assembled spring housings on top of the pump chambers. NOTE NOTE Mind the correct orientation: The backup ring must face Both spring housings are identical, there is no risk when upwards and the seal holder must sit correcly.
Page 183 Maintenance Pump Head Maintenance (Tool Free) 15 Tighten the pump head screws with a torque wrench 16 Mount the pump head to the module. Do not fix the set to approx. 5 Nm in a crosswise manner. screws at this stage! approx.
Page 184 Maintenance Pump Head Maintenance (Tool Free) 21 Counter the outlet valve and tighten the lock screw of 22 Attach the seal wash tubing interconnecting the two the heat exchanger capillary with a torque wrench set to pump heads. approx. 3 Nm. approx.
Page 185: Assemble Longlife Pump Heads (G7132A)
Maintenance Pump Head Maintenance (Tool Free) Assemble LongLife Pump Heads (G7132A) For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules. This procedure shows how to exchange seals, and reassemble the pump head assembly.
Page 186 Maintenance Pump Head Maintenance (Tool Free) Lubricate the seals, the seal holder, and the pump Place the piston seal onto the designated nose of the chambers with isopropanol. Seal Handling Device. The metal spring of the piston seal must be visible. Take care that the Seal Handling Device is seating flush Repeat for the other pump chamber.
Page 187 Maintenance Pump Head Maintenance (Tool Free) Take care that the Seal Handling Device is seating flush Repeat for the other seal holder. and press the wash seal into the seal holder. NOTE The Seal Handling Device has a cavity to fit over the pins of the seal wash tubings.
Page 188 Maintenance Pump Head Maintenance (Tool Free) 11 Place the seal holder onto the spring housing. 12 Place the assembled spring housings on top of the pump chambers. NOTE NOTE Mind the correct orientation: The backup ring must face Both spring housings are identical, there is no risk when upwards and the seal holder must sit correctly.
Page 189 Maintenance Pump Head Maintenance (Tool Free) 15 Tighten the pump head screws with a torque wrench 16 Mount the pump head to the module. Do not fix the set to approx. 5 Nm in a crosswise manner. screws at this stage! approx.
Page 190 Maintenance Pump Head Maintenance (Tool Free) 21 Counter the outlet valve and tighten the lock screw of 22 Attach the seal wash tubing interconnecting the two the heat exchanger capillary with a torque wrench set to pump heads. approximately 3 Nm. 23 Insert the screws that later fix the pump head assembly to the module housing.
Page 191: Install The Pump Head Assembly (G7120A)
Maintenance Install the Pump Head Assembly (G7120A) Install the Pump Head Assembly (G7120A) Tools required Description G7120-68708 HPLC System Tool Kit-Infinity-II 5067-5688 Torque wrench 1 – 25 Nm with 14 mm wrench G4220-20013 4 mm hex bit G4220-20015 Adapter ¼ in square to hex Bring the pump drive to the maintenance position using C AU T I O N the Lab Advisor user interface: Go to Service &...
Page 192 Maintenance Install the Pump Head Assembly (G7120A) Connect the degassing unit outlet to the inlet of the pri- Connect the outlet of the secondary pump head to the mary pump head. inlet of the purge valve. NOTE Channel A (left pump head assembly) is connected to port 4, channel B (right pump head assembly) to port 1 of the purge valve.
Page 193: Install The Pump Head Assembly (G7132A)
Maintenance Install the Pump Head Assembly (G7132A) Install the Pump Head Assembly (G7132A) For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only. Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules. Tools required Description G7120-68708...
Page 194 Maintenance Install the Pump Head Assembly (G7132A) Connect the degassing unit outlet to the inlet of the pri- Connect the outlet of the secondary pump head to the mary pump head. inlet of the purge valve. NOTE Channel A (left pump head assembly) is connected to port 4, channel B (right pump head assembly) to port 1 of the purge valve.
Page 195: Replace The Outlet Valve (G7120A)
Maintenance Replace the Outlet Valve (G7120A) Replace the Outlet Valve (G7120A) When If Outlet valve is defective. Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch Spanner-double open ended 14 mm Parts required Description G4220-60028 Outlet valve (primary pump head) G4220-20020 Internal gold seal for Outlet Valve Preparations...
Page 196 Maintenance Replace the Outlet Valve (G7120A) Remove the heat exchanger capillary by pushing the Unscrew the outlet valve with a 14 mm wrench (1) and connector up and pulling it out of the valve. remove it (2). NOTE A gold seal between outlet valve and heat exchanger capil- lary is used for a tight connection.
Page 197 Maintenance Replace the Outlet Valve (G7120A) Counter the outlet valve and tighten the lock screw of Next Steps: the heat exchanger capillary with a torque wrench set to approx. 3 Nm. 10 Place the cap on the Outlet Valve. approx. 11 Install the pump head assembly, reconnect all hydraulic 3 Nm connections, and power up the pump.
Page 198: Replace The Outlet Valve (G7132A)
Maintenance Replace the Outlet Valve (G7132A) Replace the Outlet Valve (G7132A) When If Outlet valve is defective. Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch Spanner-double open ended 14 mm Parts required Description G7131-60028 Outlet Valve Bio-Compatible Close the shut off valves to avoid solvent leaks. Open the lock screw of the heat exchanger capillary.
Page 199 Maintenance Replace the Outlet Valve (G7132A) Insert the outlet valve into the pump head (1). Using a Connect the heat exchanger capillary to the outlet valve. torque wrench, set approx. 10 Nm and close the outlet valve (2). Next Steps: Tighten the screw of the heat exchanger capillary with a torque wrench set to approx.
Page 200: Replace The Purge Valve Head
Maintenance Replace the Purge Valve Head Replace the Purge Valve Head When In case of problems with the purge valve Parts required Description 5067-4236 Purge valve head 5067-4655 Capillary ST 0.25 mm x 250 mm G4220-87000 Capillary ST 0.17 mm x 300 mm 01090-87308 Capillary ST 0.25 mm x 130 mm 5067-6736...
Page 201 Maintenance Replace the Purge Valve Head Remove all capillary connections. Unscrew the black union nut. Remove the head of the purge valve by pulling it to the Put the new valve head onto the valve drive such that front. the lobe fits to the groove. Screw the valve head onto the valve drive using the union nut.
Page 202: Replace Parts Of The Purge Valve Head
Maintenance Replace Parts of the Purge Valve Head Replace Parts of the Purge Valve Head Preparations Remove all capillary connections from the purge valve head. Use the 9/64 inch hex key for opening the valve head. Replace parts as required. Reassemble the valve head and mount it to the valve Fix the valve head with the screws.
Page 203: Replace Parts Of The High Pressure Filter Assembly (G7120A)
Maintenance Replace Parts of the High Pressure Filter Assembly (G7120A) Replace Parts of the High Pressure Filter Assembly (G7120A) When For removing blockages and repairing leaks in the high pressure filter assembly. The filter frit in the outlet valve should be replaced regularly depending on the system usage. Tools required Description 5067-5688...
Page 204 Maintenance Replace Parts of the High Pressure Filter Assembly (G7120A) Replace the filter frit and seal cap as desired. Please Re-install the filter assembly using the torque wrench note the correct orientation of the filter frit. (14 mm) set to approx. 16 Nm. approx.
Page 205: Replace Parts Of The High Pressure Filter Assembly (G7132A)
Maintenance Replace Parts of the High Pressure Filter Assembly (G7132A) Replace Parts of the High Pressure Filter Assembly (G7132A) When For removing blockages and repairing leaks in the high pressure filter assembly. The filter frit in the outlet valve should be replaced regularly depending on the system usage. Tools required Description 5067-5688...
Page 206 Maintenance Replace Parts of the High Pressure Filter Assembly (G7132A) Replace the filter frit and seal cap as desired. Please Reinstall the filter assembly using the torque wrench note the correct orientation of the filter frit. (14 mm hex bit) set to approx. 16 Nm. approx.
Page 207: Replace The Module Firmware
Maintenance Replace the Module Firmware Replace the Module Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary •...
Page 208: Prepare The Pump Module For Transport
Maintenance Prepare the Pump Module for Transport Prepare the Pump Module for Transport When If the module shall be transported or shipped. Parts required Description 9301-0411 Syringe; Plastic 9301-1337 Syringe adapter G7120-44000 Protective Foam Preparations Flush both solvent channels with isopropanol, see Best Practices for Using an Agilent LC System Technical Note.
Page 209 Maintenance Prepare the Pump Module for Transport 1 Flush system with appropriate storage solution, for example 20 % isopropanol in water. 2 Remove solvent inlets from solvent reservoirs. Disconnect the solvent tubing from the inlet of primary pump heads for both solvent channels. Use a syringe for removing liquid from the solvent tubings between solvent reservoir, shutoff valve panel, solvent selection valve, degassing unit and pump inlets.
Page 210 Maintenance Prepare the Pump Module for Transport 4 Remove the shutoff valve panel by pulling it downwards. 5 You may keep internal tubing and capillary connections. Jet Weaver Solvent selection valve Degasser Pump heads Purge valve Pressure sensor 6 Remove cable connections to other modules. Remove the module from the stack.
Page 211 Maintenance Prepare the Pump Module for Transport 7 Carefully insert the Protective Foam to the front part of the instrument. Do not damage any tubing or capillary connections. 8 Close the front cover. 9 For transport or shipment, put the module and accessory kit in the original shipment box.
Page 212: Parts And Materials For Maintenance
Parts and Materials for Maintenance Overview of Maintenance Parts (G7120A) Overview of Maintenance Parts (G7132A) Flow Connections (G7120A) Flow Connections (G7132A) Pump Heads (G7120A) Pump Head Assembly Parts Primary Pump Head Parts Secondary Pump Head Parts Pump Heads (G7132A) Pump Head Assembly Parts Primary Pump Head Parts Secondary Pump Head Parts Purge Valve (G7120A)
Page 213: Overview Of Maintenance Parts (G7120A)
Parts and Materials for Maintenance Overview of Maintenance Parts (G7120A) Overview of Maintenance Parts (G7120A) Figure 15 Overview of main assemblies (G7120A) Item Description G4220-60027 Jet Weaver 35 µL/100 µL G4220-60012 Jet Weaver 380 µL (OPTIONAL) G7120-60029 SSV Valve Assembly 5065-4445 Peristaltic pump with PharMed tubing G7104-60001...
Page 214: Overview Of Maintenance Parts (G7132A)
Parts and Materials for Maintenance Overview of Maintenance Parts (G7132A) Overview of Maintenance Parts (G7132A) Figure 16 Overview of main assemblies (G7132A) 1290 Infinity II High-Speed Pumps User Manual...
Page 215 Parts and Materials for Maintenance Overview of Maintenance Parts (G7132A) Item Description G7132-60135 Jet Weaver 35 µL/100 µL Bio-Compatible G7120-60029 SSV Valve Assembly 5065-4445 Peristaltic pump with PharMed tubing 5067-7010 Pressure sensor Bio-Compatible 1300 bar 5067-6736 Bio-Compatible purge valve head G4220-60350 Long Life Pump Head Channel A G4220-60360...
Page 216: Flow Connections (G7120A)
Parts and Materials for Maintenance Flow Connections (G7120A) Flow Connections (G7120A) Figure 17 Flow connections of the High-Speed Pump (G7120A) 1290 Infinity II High-Speed Pumps User Manual...
Page 217 Parts and Materials for Maintenance Flow Connections (G7120A) Item Description 5067-4655 Capillary ST 0.25 mm x 250 mm purge valve to pump head assemblies channel A and B 5065-4445 Peristaltic pump with PharMed tubing 3, 5 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m G4220-60035 Tubing kit 140 mm, 2/pk SSV to shutoff valve or degassing unit...
Page 218: Flow Connections (G7132A)
Parts and Materials for Maintenance Flow Connections (G7132A) Flow Connections (G7132A) Figure 18 Flow connections of the High-Speed Pump (G7132A) 1290 Infinity II High-Speed Pumps User Manual...
Page 219 Parts and Materials for Maintenance Flow Connections (G7132A) Item Description 5500-1420 Bio-Compatible capillary MP35N 0.25 mm x 250 mm purge valve to pump head assemblies channel A and B 5065-4445 Peristaltic pump with PharMed tubing 3, 5 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m G4220-60035 Tubing kit 140 mm, 2/pk SSV to shutoff valve or degassing unit...
Page 220: Pump Heads (G7120A)
Parts and Materials for Maintenance Pump Heads (G7120A) Pump Heads (G7120A) The following pages contain parts information for LongLife Pump Heads. For parts information on other pump head types, please refer to Agilent 1290 Infinity II Easy Maintenance Pump Head Technical Note (01200-90120) and to Agilent 1290 Infinity Pump Head Maintenance Technical Note (G4220-90122).
Page 221: Pump Head Assembly Parts
Parts and Materials for Maintenance Pump Heads (G7120A) Pump Head Assembly Parts Long Life Pump Head Channel A (G4220-60350) Item Description G4220-60660 Secondary Pump Head Assembly Pendulum G4220-60661 Primary Pump Head Assembly Pendulum G4220-60022 Inlet valve (primary pump head) G4220-60028 Outlet valve (primary pump head) 5042-9966...
Page 222 Parts and Materials for Maintenance Pump Heads (G7120A) Long Life Pump Head Channel B (G4220-60360) Item Description G4220-60660 Secondary Pump Head Assembly Pendulum G4220-60661 Primary Pump Head Assembly Pendulum G4220-60022 Inlet valve (primary pump head) G4220-60028 Outlet valve (primary pump head) 5042-9966 Cap Outlet Valve G4280-60026...
Page 223: Primary Pump Head Parts
Parts and Materials for Maintenance Pump Heads (G7120A) Primary Pump Head Parts Primary Pump Head Assembly Pendulum (G4220-60661) Item Description 5067-5975 Plunger Assy ZrO 0515-6154 Screw-Socket-HD-Cap Hex-Recess M5X0.8 40 G4220-60046 Preload-Support Assembly LL 0905-1175 Wash seal (PTFE) G4220-60616 Seal Holder Integrated Assembly EM/LL 0905-1719 PE Seal G4220-60533...
Page 224: Secondary Pump Head Parts
Parts and Materials for Maintenance Pump Heads (G7120A) Secondary Pump Head Parts Secondary Pump Head Assembly Pendulum (G4220-60660) Item Description 5067-5975 Plunger Assy ZrO 0515-6154 Screw-Socket-HD-Cap Hex-Recess M5X0.8 40 G4220-60046 Preload-Support Assembly LL 0905-1175 Wash seal (PTFE) G4220-60616 Seal Holder Integrated Assembly EM/LL 0905-1719 PE Seal G4220-25513...
Page 225: Pump Heads (G7132A)
Parts and Materials for Maintenance Pump Heads (G7132A) Pump Heads (G7132A) Pump Head Assembly Parts Figure 19 Bio-Compatible Pump Head Channel A Bio-Compatible Pump Head Channel A Item Description G7132-60640 Secondary Pump Head Assembly Bio-Compatible G7131-60641 Primary Pump Head Assembly Bio-Compatible G7131-60021 Inlet Valve Bio-Compatible (primary pump head)
Page 226 Parts and Materials for Maintenance Pump Heads (G7132A) Figure 20 Bio-Compatible Pump Head Channel B Bio-Compatible Pump Head Channel B Item Description G7132-60640 Secondary Pump Head Assembly Bio-Compatible G7131-60641 Primary Pump Head Assembly Bio-Compatible G7131-60021 Inlet Valve Bio-Compatible (primary pump head) G7131-60028 Outlet Valve Bio-Compatible (primary pump head)
Page 227: Primary Pump Head Parts
Parts and Materials for Maintenance Pump Heads (G7132A) Primary Pump Head Parts Figure 21 Primary pump head parts (G7132A) Primary Pump Head Assembly Bio-Compatible (G7131-60641) Item Description 5067-5975 Plunger Assy ZrO 0515-6154 Screw-Socket-HD-Cap Hex-Recess M5X0.8 40 G4220-60046 Preload-Support Assembly LL 0905-1731 Bio-Inert Wash Seal G7131-60616...
Page 228: Secondary Pump Head Parts
Parts and Materials for Maintenance Pump Heads (G7132A) Secondary Pump Head Parts Figure 22 Secondary pump head parts (G7132A) Secondary Pump Head Assembly Bio-Compatible (G7132-60640) Item Description 5067-5975 Plunger Assy ZrO 0515-6154 Screw-Socket-HD-Cap Hex-Recess M5X0.8 40 G4220-60046 Preload-Support Assembly LL 0905-1731 Bio-Inert Wash Seal G7131-60616...
Page 229: Purge Valve (G7120A)
Parts and Materials for Maintenance Purge Valve (G7120A) Purge Valve (G7120A) Item Description 5067-4236 Purge valve head 1535-4857 Stator screws 5068-0004 Purge Valve Stator 5068-0201 Purge Valve Rotor Seal, PEEK, 1300 bar 1535-4045 Bearing ring 1290 Infinity II High-Speed Pumps User Manual...
Page 230: Purge Valve (G7132A)
Parts and Materials for Maintenance Purge Valve (G7132A) Purge Valve (G7132A) Item Description 5067-6736 Bio-Compatible purge valve head 5428-0006 Stator screws 5068-0376 Purge Valve Stator Bio-Compatible 5068-0377 Purge Valve Rotor Seal, MP35N, 1300 bar Bio-Compatible 1290 Infinity II High-Speed Pumps User Manual...
Page 231: Cover Parts
Parts and Materials for Maintenance Cover Parts Cover Parts Description G7120-68713 Cabinet Kit Infinity II 200 (including top, bottom, and side + 1260 and 1290 Name Plates) 5043-0856 Leak Adapter (not shown) 5067-5767 Door assy 200 left IF II 5067-5768 Door assy 200 right IF II 1290 Infinity II High-Speed Pumps User Manual...
Page 232: Accessory Kit (G7120A)
Parts and Materials for Maintenance Accessory Kit (G7120A) Accessory Kit (G7120A) Accessory kit (G7120-68705) contains the following parts: Description G4220-67000 Waste Tubing with Fitting 5043-1013 Tubing Clip 5067-4124 Shutoff valve 5500-1246 Capillary ST 0.17 mm x 500 mm SI/SI 5181-1519 CAN cable, Agilent module to module, 1 m 5500-1155 Tube Connector, 90 degree, ID 6.4...
Page 233: Accessory Kit (G7132A)
Parts and Materials for Maintenance Accessory Kit (G7132A) Accessory Kit (G7132A) Accessory kit Bio-Compatible (G7132-68705) contains the following parts: Description G4220-67000 Waste Tubing with Fitting 5043-1013 Tubing Clip 5067-4124 Shutoff valve 5500-1419 Capillary MP35N 0.17 mm x 500 mm, SI/SI 5181-1519 CAN cable, Agilent module to module, 1 m 5500-1155...
Page 234: Hplc System Tool Kit
Parts and Materials for Maintenance HPLC System Tool Kit HPLC System Tool Kit 5023-2504 (Hex Driver SW-4 slitted) 5023-2503 5023-3138 (Hex Driver (Reversible Screwdriver) SW-5 slitted) 5023-2502 (Hex Driver SW-6, 35/ ¼” slitted) 5023-3088 8710-1924 8710-2409 8710-0510 8710-0510 5023-2500 8710-1534 8710-2394 (Hex Key 9/64“, 15cm) Box with:...
Page 235: Identifying Cables
Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables Agilent Module to PC This chapter provides information on cables used with the modules. 1290 Infinity II High-Speed Pumps User Manual...
Page 236: Cable Overview
Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter 01046-60105 Analog cable (BNC to general purpose, spade lugs)
Page 237 Identifying Cables Cable Overview LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connec- tion) RS-232 cables (not for Description FUSION board) RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female).
Page 238: Analog Cables
Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
Page 239 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog +...
Page 240: Remote Cables
Identifying Cables Remote Cables Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male - male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 Color code Enhanced...
Page 241 Identifying Cables Remote Cables • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding...
Page 242 High Ground Cable Shield- One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. 1290 Infinity II High-Speed Pumps User Manual...
Page 243 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue...
Page 244: Can/Lan Cables
Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...
Page 245: Agilent Module To Pc
Identifying Cables Agilent Module to PC Agilent Module to PC Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 246: Usb
Identifying Cables To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) 1290 Infinity II High-Speed Pumps User Manual...
Page 247: Hardware Information
Hardware Information Firmware Description Electrical Connections Serial Number Information Rear View of the Module Interfaces Overview Interfaces USB (Universal Serial Bus) Setting the 8-bit Configuration Switch Special Settings Early Maintenance Feedback Instrument Layout This chapter describes the pump in more detail on hardware and electronics. 1290 Infinity II High-Speed Pumps User Manual...
Page 248: Firmware Description
Hardware Information Firmware Description Firmware Description The firmware of the instrument consists of two independent sections: • a non-instrument specific section, called resident system • an instrument specific section, called main system Resident System This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules.
Page 249 Hardware Information Firmware Description Firmware Updates Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used). Required tools, firmware and documentation are available from the Agilent web: http://www.agilent.com/en-us/firmwareDownload?whid=69761 The file naming conventions are: PPPP_RVVV_XXX.dlb, where •...
Page 250 Hardware Information Firmware Description Some modules are limited in downgrading due to their mainboard version or NOTE their initial firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware revision B.01.02 or to a A.xx.xx. Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in specific control software environments.
Page 251: Electrical Connections
There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations.
Page 252: Rear View Of The Module
Hardware Information Electrical Connections Rear View of the Module APG Remote Power supply for external CAN valves Configuration switch CAN Bus RS232 Power plug Figure 24 Rear view of the High-Speed Pump 1290 Infinity II High-Speed Pumps User Manual...
Page 253: Interfaces
Hardware Information Interfaces Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 20 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A, G7132A G7161A/B...
Page 254 Hardware Information Interfaces Table 20 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Fraction Collectors G7158B G7159B G7166A Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with addi-...
Page 255: Overview Interfaces
Hardware Information Interfaces Overview Interfaces The CAN is inter-module communication interface. It is a 2-wire serial bus system supporting high speed data communication and real-time requirement. The modules have either an interface slot for a LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on-board LAN interface (e.g.
Page 256 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 257: Usb (Universal Serial Bus)
Hardware Information Interfaces Table 21 ERI signal distribution Signal Description START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler. STOP (L) Request to reach system ready state as soon as possible (for example, stop run, abort or finish and stop injection).
Page 258: Setting The 8-Bit Configuration Switch
Hardware Information Setting the 8-bit Configuration Switch Setting the 8-bit Configuration Switch The 8-bit configuration switch is located at the rear of the module. Switch settings provide configuration parameters for LAN, serial communication protocol and instrument specific initialization procedures. All modules with on-board LAN: •...
Page 259 Hardware Information Setting the 8-bit Configuration Switch Table 22 8-bit Configuration Switch (with on-board LAN) Mode Function SW 1 SW 2 SW 3 SW 4 SW 5 SW 6 SW 7 SW 8 Link Configuration Init Mode Selection Auto-negotiation 10 MBit, half-duplex 10 MBit, full-duplex 100 MBit, half-duplex 100 MBit, full-duplex...
Page 260: Special Settings
Hardware Information Setting the 8-bit Configuration Switch Special Settings The special settings are required for specific actions (normally in a service case). The tables include both settings for modules – with on-board LAN and without NOTE on-board LAN. They are identified as LAN and no LAN. Boot-Resident Firmware update procedures may require this mode in case of firmware loading errors (main firmware part).
Page 261: Early Maintenance Feedback
Hardware Information Early Maintenance Feedback Early Maintenance Feedback Maintenance requires the exchange of components that are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of use of the module and the analytical conditions, and not on a predefined time interval.
Page 262: Instrument Layout
Hardware Information Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.
Page 263: Lan Configuration
LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switch Initialization mode selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Link configuration selection Manual Configuration With Telnet PC and User Interface Software Setup Setup PC Setup for Local Configuration User Interface Software Setup This chapter provides information on connecting the module to the Agilent...
Page 264: What You Have To Do First
LAN Configuration What You Have to Do First What You Have to Do First The module has an on-board LAN communication interface. 1 Note the MAC (Media Access Control) address for further reference. The MAC or hardware address of the LAN interfaces is a world wide unique identifier. No other network device will have the same hardware address.
Page 265: Tcp/Ip Parameter Configuration
LAN Configuration TCP/IP parameter configuration TCP/IP parameter configuration To operate properly in a network environment, the LAN interface must be configured with valid TCP/IP network parameters. These parameters are: • IP address • Subnet Mask • Default Gateway The TCP/IP parameters can be configured by the following methods: •...
Page 266: Configuration Switch
LAN Configuration Configuration Switch Configuration Switch The configuration switch can be accessed at the rear of the module. Configuration switch Figure 28 Location of Configuration Switch The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF. NOTE Table 25 Factory Default Settings...
Page 267: Initialization Mode Selection
LAN Configuration Initialization mode selection Initialization mode selection The following initialization (init) modes are selectable: Table 26 Initialization Mode Switches SW 6 SW 7 SW 8 Init Mode Using Stored Using Default DHCP Requires firmware B.06.40 or above. Modules without LAN on board, see G1369C LAN Interface Card Using Stored When initialization mode Using Stored is selected, the parameters are taken from the non-volatile memory of the module.
Page 268 LAN Configuration Initialization mode selection Using Default When Using Default is selected, the factory default parameters are taken instead. These parameters enable a TCP/IP connection to the LAN interface without further configuration, see Table 27 on page 268. Default Active Parameter Parameter Figure 30...
Page 269: Dynamic Host Configuration Protocol (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) The Dynamic Host Configuration Protocol (DHCP) is an auto configuration protocol used on IP networks. The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware.
Page 270 LAN Configuration Dynamic Host Configuration Protocol (DHCP) Setup (DHCP) The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware. All mod- ules should use latest firmware from the same set. 1 Note the MAC address of the LAN interface (provided with G1369C LAN Interface Card or mainboard).
Page 271 LAN Configuration Dynamic Host Configuration Protocol (DHCP) 2 Set the configuration switch to DHCP either on the G1369C LAN Interface Card or the mainboard of above mentioned modules. Table 28 G1369C LAN Interface Card (configuration switch on the card) SW 4 SW 5 SW 6 SW 7...
Page 272: Link Configuration Selection
LAN Configuration Link configuration selection Link configuration selection The LAN interface supports 10 or 100 Mbps operation in full- or half-duplex modes. In most cases, full-duplex is supported when the connecting network device - such as a network switch or hub - supports IEEE 802.3u auto-negotiation specifications.
Page 273: Manual Configuration
LAN Configuration Manual Configuration Manual Configuration Manual configuration only alters the set of parameters stored in the non-volatile memory of the module. It never affects the currently active parameters. Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing it.
Page 274: With Telnet
LAN Configuration Manual Configuration With Telnet Whenever a TCP/IP connection to the module is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. 1 Open the system (DOS) prompt window by clicking on Windows START button and select “Run...”.
Page 275 LAN Configuration Manual Configuration 3 Type ? and press enter to see the available commands. Figure 36 Telnet Commands Table 31 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip <x.x.x.x> sets new ip address sm <x.x.x.x>...
Page 276 LAN Configuration Manual Configuration 5 Use the “/” and press Enter to list the current settings. information about the LAN interface MAC address, initialization mode Initialization mode is Using Stored active TCP/IP settings TCP/IP status - here ready connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 37 Telnet - Current settings in "Using Stored"...
Page 277 LAN Configuration Manual Configuration 7 When you have finished typing the configuration parameters, type exit and press Enter to exit with storing parameters. Figure 39 Closing the Telnet Session If the Initialization Mode Switch is changed now to “Using Stored” mode, the NOTE instrument will take the stored settings when the module is re-booted.
Page 278: Pc And User Interface Software Setup Setup
LAN Configuration PC and User Interface Software Setup Setup PC and User Interface Software Setup Setup PC Setup for Local Configuration This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see also “Initialization mode selection”...
Page 279: User Interface Software Setup
LAN Configuration PC and User Interface Software Setup Setup User Interface Software Setup Install you user interface software according the provided User Interface Software Setup Guide. 1290 Infinity II High-Speed Pumps User Manual...
Page 280: Appendix
Safety Symbols Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Certification for Federal Republic of Germany Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. 1290 Infinity II High-Speed Pumps User Manual...
Page 281: General Safety Information
Appendix General Safety Information General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
Page 282: Before Applying Power
Verify that the voltage range and frequency of your power distribution  matches to the power specification of the individual instrument. Never use cables other than the ones supplied by Agilent Technologies  to ensure proper functionality and compliance with safety or EMC regulations.
Page 283: Do Not Operate In An Explosive Atmosphere
Appendix General Safety Information Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WAR N IN G Explosion hazard Do not operate the instrument in the presence of flammable gases or  fumes. Do Not Remove the Instrument Cover Instrument covers removed WAR N IN G Electrical shock...
Page 284: Solvents
Appendix General Safety Information Solvents Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety  procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 285: Safety Symbols
Appendix General Safety Information Safety Symbols Table 32 Symbols The apparatus is marked with this symbol when the user shall refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages.
Page 286 Appendix General Safety Information Table 32 Symbols Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.
Page 287: Waste Electrical And Electronic Equipment (Weee) Directive
Appendix Waste Electrical and Electronic Equipment (WEEE) Directive Waste Electrical and Electronic Equipment (WEEE) Directive This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste NOTE To return unwanted products, contact your local Agilent office, or see...
Page 288: Radio Interference
Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
Page 289: Sound Emission Certification For Federal Republic Of Germany
Appendix Sound Emission Certification for Federal Republic of Germany Sound Emission Certification for Federal Republic of Germany Sound pressure Sound pressure Lp <70 db(A) accroding to DIN-EN 27779 Schalldruckpegel Schalldruckpegel Lp <70 db(A) nach DIN-EN 27779 1290 Infinity II High-Speed Pumps User Manual...
Page 290: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com 1290 Infinity II High-Speed Pumps User Manual...
Page 291 • introduction and specifications, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • hardware information, • safety and related information. www.agilent.com  Agilent Technologies Inc. 2014-2020 Published in Germany 09/2020 Document No: SD-29000230 Rev. E...

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