Agilent Technologies InfinityLab LC Series User Manual
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Agilent Technologies InfinityLab LC Series User Manual

Preparative binary pumps
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  • Page 1 Agilent InfinityLab LC Series Preparative Binary Pumps User Manual Preparative Binary Pumps User Manual...
  • Page 2 CAUTION beyond a notice until ble for errors or for incidental or conse- sent from Agilent Technologies, Inc. as the indicated conditions are fully quential damages in connection with the governed by United States and interna- understood and met.
  • Page 3 In This Guide... This manual covers the following modules: • Agilent 1260 Infinity II Preparative Binary Pump (G7161A) • Agilent 1290 Infinity II Preparative Binary Pump (G7161B) Introduction This chapter gives an introduction to the module and an instrument overview. Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
  • Page 4 Parts and Materials for Maintenance This chapter provides information on parts for maintenance. Identifying Cables This chapter provides information on cables used with the modules. Hardware Information This chapter describes the module in more detail on hardware and electronics. LAN Configuration This chapter provides information on connecting the module to the Agilent ChemStation PC.

  • Page 5: Table Of Contents

    Contents Introduction G7161A Preparative Binary Pump G7161B Preparative Binary Pump Flow Reduction Pump Principle Leak and Waste Handling Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Using the Pump Magnets Turn on/off Status Indicators Best Practices Purging the Pump Solvent Information Algae Growth in HPLC Systems Agilent Local Control Modules...
  • Page 6 Agilent Lab Advisor Software Pump Leak Rate Test Error Information What Are Error Messages General Error Messages Module Error Messages Maintenance Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Install Fittings and Capillaries Remove and Install Doors Open and Close Doors Replace the Manifold Replace the Y-Connector (G7161A)
  • Page 7 Parts and Materials for Maintenance Overview of Maintenance Parts (G7161A) Overview of Maintenance Parts (G7161B) Flow Connections (G7161A) Flow Connections (G7161B) Purge Valve Pump Heads Accessory Kit HPLC System Tool Kit Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables Agilent Module to PC Hardware Information Firmware Description...
  • Page 8 Dynamic Host Configuration Protocol (DHCP) Manual Configuration Appendix General Safety Information Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Agilent Technologies on Internet Preparative Binary Pumps User Manual...

  • Page 9: Introduction

    Introduction G7161A Preparative Binary Pump Product Description Features G7161B Preparative Binary Pump Product Description Features Flow Reduction Flow Reduction when Column Switching Flow Reduction for Pump Protection (G7161B only) Pump Principle Leak and Waste Handling Leak Sensor This chapter gives an introduction to the module and an instrument overview. Preparative Binary Pumps User Manual...

  • Page 10: G7161A Preparative Binary Pump

    Introduction G7161A Preparative Binary Pump G7161A Preparative Binary Pump Product Description The 1260 Infinity II Preparative Binary Pump is an affordable high-pressure gradient pump for LC purification. This pump has been designed and tested for reverse-phase solvents and engineered for continuous use to provide reliable and rugged performance through a dual-piston, rapid-refill design.

  • Page 11: Features

    Introduction G7161A Preparative Binary Pump Features Purification efficiency • Outstanding retention time stability for routine operation Instrument efficiency • Dynamic flow range up to 50 mL/min at 420 bar for semi-preparative applications • Outstanding binary gradient compositional accuracy across a wide dynamic flow range Laboratory efficiency •...

  • Page 12: G7161B Preparative Binary Pump

    Introduction G7161B Preparative Binary Pump G7161B Preparative Binary Pump Product Description The 1290 Infinity II Preparative Binary Pump is an affordable high-pressure gradient pump for LC purification. This pump has been designed and tested for reverse-phase solvents and engineered for continuous use to provide reliable and rugged performance through a dual-piston, rapid-refill design.

  • Page 13: Features

    Introduction G7161B Preparative Binary Pump Features Purification efficiency • Outstanding retention time stability for routine operation Instrument efficiency • Exchangeable pump heads deliver maximum efficiency and performance across the entire flow range • Dynamic flow range up to 50 mL/min at 600 bar using the analytical to preparative pump head •...

  • Page 14: Flow Reduction

    Introduction Flow Reduction Flow Reduction Flow Reduction when Column Switching In a pressure cluster hosted by a Preparative Binary Pump, the flow reduction feature protects the instrument and column from pressure spikes caused by a switching valve by reducing the flow rate before switching the valve. The flow reduction sequence is as follows: 1 A valve switch command is issued by the user or method.

  • Page 15: Pump Principle

    Introduction Pump Principle Pump Principle Pump Principle of the 1260 Infinity II Preparative Binary Pump (G7161A): The solvent flow leaving the two pump heads on each channel is combined by a t-piece before passing through the manifold, which combines the flow from both pump channels.
  • Page 16 Introduction Pump Principle To sampler Seal wash solvent Y-connector Seal wash pump Pump head Pump head Manifold Pump head Pump head Pressure sensor Purge valve Mixer Figure 3 Hydraulic path of the pump (G7161A) Preparative Binary Pumps User Manual...
  • Page 17 Introduction Pump Principle Solvent Sampler Seal wash solvent Seal wash sensor Solvent selection Seal wash pump valve Pump head Pump head Mixer Pump head Pump head Manifold Pressure sensor Purge valve Figure 4 Hydraulic path of the pump (G7161B) Preparative Binary Pumps User Manual...

  • Page 18: Leak And Waste Handling

    Introduction Leak and Waste Handling Leak and Waste Handling 1 The figure below shows the leak and waste drain outlets of the module. Each module must have its own waste line. Waste lines cannot be combined. NOTE Leak Sensor Solvent incompatibility C AU T I O N The solvent DMF (dimethyl formamide) leads to corrosion of the leak sensor.

  • Page 19: Site Requirements And Specifications

    Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications Performance Specifications (G7161A) Performance Specifications (G7161B) This chapter provides information on environmental requirements, physical and performance specifications. Preparative Binary Pumps User Manual...

  • Page 20: Site Requirements

    Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the instrument. Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Table 1 on page 24.
  • Page 21 Never use a power cord other than the one that Agilent shipped with this  instrument. Never use the power cords that Agilent Technologies supplies with this  instrument for any other equipment. Never use cables other than the ones supplied by Agilent Technologies ...
  • Page 22 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 1 on page 24) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections.
  • Page 23 Site Requirements and Specifications Site Requirements Thermal Equilibration Inadequate Thermal Equilibration C AU T I O N Inadequate equilibration can cause damage to the system.  Ensure the pump is kept at operating conditions for 24 hours before switching it on. Preparative Binary Pumps User Manual...

  • Page 24: Physical Specifications

    Site Requirements and Specifications Physical Specifications Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 27.2 kg Dimensions 320 x 396 x 436 mm (height × width × depth) (12.6 x 15.6 x 17.2 inches) Line voltage 100 – 240 V~, ± 10 % Wide-ranging capability Line frequency 50 or 60 Hz, ±...

  • Page 25: Performance Specifications

    Site Requirements and Specifications Performance Specifications Performance Specifications Performance Specifications (G7161A) Table 2 Agilent 1260 Infinity II Preparative Binary Pump (G7161A) Performance Specifications Feature Specification Comment Settable flow range 0.01 – 50 mL/min, in 0.01 mL/min increments Pressure operating range Up to 42 MPa (420 bar, 6092 psi) Compressibility compensation...
  • Page 26 Site Requirements and Specifications Performance Specifications Table 2 Agilent 1260 Infinity II Preparative Binary Pump (G7161A) Performance Specifications Feature Specification Comment ≤0.3 % RSD or ≤0.1 min SD Prep LC Standard #1 Composition precision whichever is greater, based on (5190-6886) from 2 – 98 % retention time at constant room Acetonitrile temperature...
  • Page 27 Site Requirements and Specifications Performance Specifications Table 2 Agilent 1260 Infinity II Preparative Binary Pump (G7161A) Performance Specifications Feature Specification Comment GLP features Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with pre-defined and user settable limits and feedback messages.

  • Page 28: Performance Specifications (G7161B)

    Site Requirements and Specifications Performance Specifications Performance Specifications (G7161B) Table 3 Agilent 1290 Infinity II Preparative Binary Pump (G7161B) Performance Specifications Feature Specification Comment Settable flow range 50 mL head:0.01 – 50 mL/min, in 0.01 mL/min increments 200 mL head:0.01 – 200 mL/min, in 0.01 mL/min increments Recommended Flow range 50 mL head: ≥...
  • Page 29 Site Requirements and Specifications Performance Specifications Table 3 Agilent 1290 Infinity II Preparative Binary Pump (G7161B) Performance Specifications Feature Specification Comment <±1.0 % 50 mL head: Flow rate Flow accuracy 1 – 50 mL/min, 200 mL head: Flow rate 4 – 150 ml/min using 100 % Channel A or B with Water or MeOH ±1.0 % from 2 –...
  • Page 30 Site Requirements and Specifications Performance Specifications Table 3 Agilent 1290 Infinity II Preparative Binary Pump (G7161B) Performance Specifications Feature Specification Comment Communications Controller-area network (CAN), Local Area Network (LAN), Extended remote interface (ERI), USB, ready, start, stop and shutdown signals, external leak sensor.

  • Page 31: Using The Pump

    Using the Pump Magnets Turn on/off Status Indicators Best Practices Regular Inspections Power-up / Shut-down Prime the Pump Prepare the pump Using the Pump Purging the Pump Solvent Information Algae Growth in HPLC Systems How to Prevent and-or Reduce the Algae Problem Agilent Local Control Modules This chapter explains the operational parameters of the module.

  • Page 32: Magnets

    Using the Pump Magnets Magnets 1 Magnets in doors of pumps, autosamplers, detectors, and fraction collectors. Preparative Binary Pumps User Manual...

  • Page 33: Turn On/Off

    Using the Pump Turn on/off Turn on/off This procedure exemplarily shows an arbitrary LC stack configuration. Power switch: On Preparative Binary Pumps User Manual...
  • Page 34 Using the Pump Turn on/off Turn instrument On/Off with the control software. Power switch: Off Preparative Binary Pumps User Manual...

  • Page 35: Status Indicators

    Using the Pump Status Indicators Status Indicators This procedure exemplarily shows an arbitrary LC stack configuration. 1 The module status indicator indicates one of six possible module conditions: Status indicators 1. Idle 2. Run mode 3. Not-ready. Waiting for a specific pre-run condition to be reached or completed.

  • Page 36: Best Practices

    Using the Pump Best Practices Best Practices Regular Inspections • Inspect the inlet/waste tubing and exchange them if they are worn out or show visible signs of damage. • Prevent blocking of solvent inlet filters (never use the pump without solvent inlet filters).

  • Page 37: Prime The Pump

    Using the Pump Best Practices Prime the Pump Always organic channel first, water channel second. NOTE 1 Attach the 50 mL syringe (in Accessory Kit) to the waste port of purge valve. 2 Open the purge valve. 3 Turn on one channel with 5 mL/min (100 % A or B). 4 Pull the syringe and hold it until all the tubing and heads within the active channel are filled.

  • Page 38: Using The Pump

    Using the Pump Best Practices Using the Pump • For the generation of gradients, make sure that none of the channels delivers less than a minimum flow rate of 50 µL/min at any time during the gradient run, in order to achieve best performance. •...

  • Page 39: Purging The Pump

    Using the Pump Purging the Pump Purging the Pump When the solvents have been exchanged or the pumping system has been turned off for a certain time (for example, overnight) oxygen will re-diffuse into the solvent channel between the solvent reservoir and the pump. Solvents containing volatile ingredients will slightly lose these.
  • Page 40 Using the Pump Purging the Pump 5 Close the Purge Configuration dialog box. When the request to open the purge valve appears, open the purge valve on the pump, then click to close the message box. During purging, the General tab shows the current channel that is being purged, and the remaining purge time.

  • Page 41: Solvent Information

    Using the Pump Solvent Information Solvent Information Introduction Observe the following recommendations on the use of solvents. • Follow recommendations for avoiding the growth of algae, see “Algae Growth in HPLC Systems” on page 49. • Small particles can permanently block capillaries and valves. Therefore, always filter solvents through 0.22 µm filters.
  • Page 42 Using the Pump Solvent Information Materials in Flow Path Following materials are used in the flow path of this module: Part Materials Bottle Head Assy SST frit, PTFE, PEEK PFA (tubings), PEEK (fittings) Ultra clean tubings Passive inlet valve SST, sapphire (1290), ruby, PEEK, ZrO -based Ceramic Outlet valve SST, sapphire (1290), ruby, PEEK, ZrO...
  • Page 43 Using the Pump Solvent Information Disclaimer Subsequent data was collected from external resources and is meant as a reference. Agilent cannot guarantee the correctness and completeness of such information. Data is based on compatibility libraries, which are not specific for estimating the long-term life time under specific but highly variable conditions of UHPLC systems, solvents, solvent mixtures and samples.
  • Page 44 Using the Pump Solvent Information Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial acetic acid, DMSO and THF. It is also degraded by nucleophilic substances like ammonia (e.g.
  • Page 45 Using the Pump Solvent Information • Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, diisopropylether). Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides. • Solutions of organic acids (acetic acid, formic acid, and so on) in organic solvents.
  • Page 46 Using the Pump Solvent Information Gold Gold is inert to all common HPLC solvents, acids and bases within the specified pH range. It can be corroded by complexing cyanides and concentrated acids like aqua regia. Zirconium Oxide (ZrO Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications.
  • Page 47 Using the Pump Solvent Information Solvent Handling Handling of Buffers The following recommendations should be observed when using buffer solutions: • Buffers and aqueous solutions are possible sources of algae contamination, for avoiding related problems, please read “Algae Growth in HPLC Systems”...
  • Page 48 Using the Pump Solvent Information When using acetonitrile: • Use high-quality solvents from renowned suppliers. • Use fresh solvents and filter them. • Minimize exposure to light and air/oxygen. • Choose a bottle size which fits to your application and usage. •...

  • Page 49: Algae Growth In Hplc Systems

    Using the Pump Algae Growth in HPLC Systems Algae Growth in HPLC Systems The presence of algae in HPLC systems can cause a variety of problems that may be incorrectly diagnosed as instrument or application problems. Algae grow in aqueous media, preferably in a pH range of 4-8. Their growth is accelerated by buffers, for example phosphate or acetate.

  • Page 50: Agilent Local Control Modules

    Using the Pump Agilent Local Control Modules Agilent Local Control Modules Agilent 1200 Infinity Series Instant Pilot G4208A The Agilent 1200 Infinity Series Instant Pilot controller gives you complete control, system monitoring, signal plotting and diagnostic capabilities for a virtually unlimited number of LC system modules. It is connected to the LC system with a CAN cable for power supply and communication.

  • Page 51: Optimizing Performance

    Optimizing Performance Delay Volume and Extra-Column Volume Delay Volume How to Achieve Higher Resolution Using Solvent Calibration Tables This chapter gives hints on how to optimize the performance or use additional devices. Preparative Binary Pumps User Manual...

  • Page 52: Delay Volume And Extra-Column Volume

    Optimizing Performance Delay Volume and Extra-Column Volume Delay Volume and Extra-Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column. The extra-column volume is defined as the volume between the injection point and the detection point, excluding the volume in the column.

  • Page 53: How To Achieve Higher Resolution

    Optimizing Performance How to Achieve Higher Resolution How to Achieve Higher Resolution Increased resolution in a separation will improve the qualitative and quantitative data analysis, allow more peaks to be separated or offer further scope for speeding up the separation. This section explains how resolution can be increased by examining the following points: •...
  • Page 54 Optimizing Performance How to Achieve Higher Resolution proportionally with the length of the column. Resolution increases with the square root of N so doubling the length of the column will increase resolution by a factor of 1.4. What is achievable depends on the viscosity of the mobile phase as this relates directly to the pressure.
  • Page 55 Optimizing Performance How to Achieve Higher Resolution This shows that k and hence resolution can be increased by having a shallower gradient (2 to 5 %/min change is a guideline), higher flow rate and a smaller volume column. This equation also shows how to speed up an existing gradient –...

  • Page 56: Using Solvent Calibration Tables

    Optimizing Performance Using Solvent Calibration Tables Using Solvent Calibration Tables RC.NET based Agilent graphical user interfaces (ChemStation, EZChrom Elite, OpenLab etc.) include data for commonly used solvents in Preparative HPLC. This data is used for optimum pump control in order to ensure best flow and composition accuracy.
  • Page 57 Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software Pump Leak Rate Test Troubleshooting the Pump Leak Rate Test Overview about the troubleshooting and diagnostic features. Preparative Binary Pumps User Manual...

  • Page 58: Troubleshooting And Diagnostics User Interfaces

    Troubleshooting and Diagnostics User Interfaces User Interfaces • Depending on the user interface, the available tests and the screens/reports may vary. • Preferred tool should be Agilent Lab Advisor Software, see “Agilent Lab Advisor Software” on page 59. • The Agilent OpenLAB ChemStation C.01.03 and above do not include any maintenance/test functions.

  • Page 59: Agilent Lab Advisor Software

    Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument. The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines.

  • Page 60: Pump Leak Rate Test

    Troubleshooting and Diagnostics Pump Leak Rate Test Pump Leak Rate Test The Pump Leak Rate Tests is a diagnostic test to check the integrity and tightness of the pump components. The test is started from the Services & Diagnostics section in the Agilent Lab Advisor Software. The test is first evaluating the tightness from the outlet valve downstream to the purge valve.
  • Page 61 Troubleshooting and Diagnostics Pump Leak Rate Test Figure 5 Pump leak rate test (example) Preparative Binary Pumps User Manual...
  • Page 62 Troubleshooting and Diagnostics Pump Leak Rate Test Troubleshooting the Pump Leak Rate Test Secondary Leak • Leak between the outlet valve and purge valve • Check for visible leaks on fittings and connectors • Connector not fixed / tight enough •...
  • Page 63 Troubleshooting and Diagnostics Pump Leak Rate Test • Outlet valve not properly assembled • Re-tighten the outlet valve • Check the position of the gold seal • Leaky piston seals and/or position dependent leaks on the piston • Remove the SW tubes from the support ring and check for leaks •...

  • Page 64: Error Information

    Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Short Leak Sensor Open Fan Failed Leak Module Error Messages Solvent Zero Counter Pressure Above Upper Limit Pressure Below Lower Limit Pressure Signal Missing Temperature Limit Exceeded Motor-Drive Power Encoder Missing...

  • Page 65: What Are Error Messages

    Error Information What Are Error Messages What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).

  • Page 66: General Error Messages

    Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout Error ID: 0062 The timeout threshold was exceeded. Probable cause Suggested actions Check the logbook for the occurrence and source The analysis was completed successfully, of a not-ready condition.

  • Page 67: Shutdown

    Error Information General Error Messages Shutdown Error ID: 0063 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.

  • Page 68: Remote Timeout

    Error Information General Error Messages Remote Timeout Error ID: 0070 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.

  • Page 69: Leak Sensor Short

    Error Information General Error Messages Leak Sensor Short Error ID: 0082 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.

  • Page 70: Leak Sensor Open

    Error Information General Error Messages Leak Sensor Open Error ID: 0083 The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak-sensor current to change within defined limits.

  • Page 71: Fan Failed

    Error Information General Error Messages Fan Failed Error ID: 0068 The cooling fan in the module has failed. The hall sensor on the fan shaft is used by the main board to monitor the fan speed. If the fan speed falls below a certain limit for a certain length of time, the error message is generated.

  • Page 72: Leak

    Error Information General Error Messages Leak Error ID: 0064 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent.

  • Page 73: Module Error Messages

    Error Information Module Error Messages Module Error Messages Solvent Zero Counter Error ID: 2055 The error message is triggered if the remaining volume in a solvent bottle falls below the set limit. Probable cause Suggested actions Refill bottles and reset solvent counters. Volume in bottle below specified volume.

  • Page 74: Pressure Below Lower Limit

    Error Information Module Error Messages Pressure Below Lower Limit Error ID: 2015, 2501 The system pressure has fallen below the lower pressure limit. Probable cause Suggested actions Ensure the lower pressure limit is set to a value Lower pressure limit set too high. suitable for the analysis.

  • Page 75: Temperature Limit Exceeded

    Error Information Module Error Messages Temperature Limit Exceeded Error ID: 2517 The temperature of one of the motor-drive circuits is too high. The processor continually monitors the temperature of the drive circuits on the main board. If excessive current is being drawn for long periods, the temperature of the circuits increases.

  • Page 76: Motor-Drive Power

    Error Information Module Error Messages Motor-Drive Power Error ID: 2041, 2042 The current drawn by the pump motor exceeded the maximum limit. Blockages in the flow path are usually detected by the pressure sensor in the damper, which result in the pump switching off when the upper pressure limit is exceeded.

  • Page 77: Encoder Missing

    Error Information Module Error Messages Encoder Missing Error ID: 2046, 2050, 2510 The optical encoder on the pump motor in the module is missing or defective. The processor checks the presence of the pump encoder connector every 2 s. If the connector is not detected by the processor, the error message is generated.

  • Page 78: Initialization Failed

    Error Information Module Error Messages Initialization Failed Error ID: 2207, 2217 The module failed to initialize successfully within the maximum time window. A maximum time is assigned for the complete pump-initialization cycle. If the time is exceeded before initialization is complete, the error message is generated.

  • Page 79: Maintenance

    Maintenance Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Install Fittings and Capillaries Remove and Install Doors Open and Close Doors Replace the Manifold Replace the Y-Connector (G7161A) Replace the Solvent Selection Valve (G7161B) Replace the Solvent Mixer Replace the Seal Wash Pump Replace the Seal Wash Sensor Replace the Purge Valve...

  • Page 80: Introduction To Maintenance

    Maintenance Introduction to Maintenance Introduction to Maintenance The figures below show the main user accessible assemblies of the preparative binary pumps. These parts can be accessed from the front (simple repairs) and don’t require to remove the pump from the system stack. Active seal wash Y-connector Channel B...
  • Page 81 Maintenance Introduction to Maintenance Seal wash sensor Active seal wash Solvent selection valve Solvent mixer Channel B Manifold Channel A Purge Valve Leak and waste drain Figure 7 Maintenance Parts (G7161B), Solvent mixer vertical Preparative Binary Pumps User Manual...
  • Page 82 Maintenance Introduction to Maintenance Seal wash sensor Active seal wash Solvent selection valve Channel B Manifold Channel A Solvent mixer Purge Valve Leak and waste drain Figure 8 Maintenance Parts (G7161B), Solvent mixer horizontal Preparative Binary Pumps User Manual...

  • Page 83: Warnings And Cautions

    Maintenance Warnings and Cautions Warnings and Cautions Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety ...
  • Page 84 Maintenance Warnings and Cautions Heavy weight WAR N IN G The module is heavy. Carry the module at least with 2 people.  Avoid back strain or injury by following all precautions for lifting heavy  objects. Ensure that the load is as close to your body as possible. ...

  • Page 85: Overview Of Maintenance

    Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the module that can be carried out without opening the main cover. Preparative Binary Pumps User Manual...

  • Page 86: Cleaning The Module

    Maintenance Cleaning the Module Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Liquid dripping into the electronic compartment of your module can cause WAR N IN G shock hazard and damage the module Do not use an excessively damp cloth during cleaning.

  • Page 87: Install Fittings And Capillaries

    Maintenance Install Fittings and Capillaries Install Fittings and Capillaries Solvent can spray under high pressure. WAR N IN G Observe appropriate safety procedures (for example, goggles, safety  gloves and protective clothing), when opening flow path. Deformation of fittings and seals C AU T I O N Liquid drops under high pressure act like solid parts.

  • Page 88: Remove And Install Doors

    Maintenance Remove and Install Doors Remove and Install Doors Parts required Description 5067-6216 Door Assembly R 5067-6217 Door Assembly L The figures shown in this procedure exemplarily show the Infinity II Multisampler NOTE module. The principle of how to remove and/or install doors works in the same way for all Infinity II modules.
  • Page 89 Maintenance Remove and Install Doors Press the release buttons and pull the front door out. For the Installation of the front door. Insert the hinges into their guides and move the door in until the release buttons click into their final position. Preparative Binary Pumps User Manual...

  • Page 90: Open And Close Doors

    Maintenance Open and Close Doors Open and Close Doors When To gain access to the front of the module as required for any maintenance procedure. Open the doors. Close the doors. Preparative Binary Pumps User Manual...

  • Page 91: Replace The Manifold

    Maintenance Replace the Manifold Replace the Manifold When In case of problems with the Manifold. Parts required Description G7161-20019 Manifold Lift up solvent filters in solvent reservoirs to avoid Remove tubing connections between the manifold and leakages. the pump head, and remove the inlet tubing line. Open the purge valve.
  • Page 92 Maintenance Replace the Manifold Install tubing connections between the manifold and Next Steps: the pump head, and install the inlet tubing line. Make sure all capillary and tubing connections are reconnected and tight. 10 Insert solvent filters into solvent reservoirs. 11 Power on the system.

  • Page 93: Replace The Y-Connector (G7161A)

    Maintenance Replace the Y-Connector (G7161A) Replace the Y-Connector (G7161A) Parts required Description 5067-6651 Y-connector assembly Lift up solvent filters in solvent reservoirs to avoid Remove the tubings from the Y-Connector. leakages. Open the purge valve. Power off the system. Open the doors. Unscrew the fixing screws (1.), lift (2.), and remove the Install the Y-Connector (1., 2.), and fix it with the screws Y-Connector.

  • Page 94: Replace The Solvent Selection Valve (G7161B)

    Maintenance Replace the Solvent Selection Valve (G7161B) Replace the Solvent Selection Valve (G7161B) Parts required Description G7161-60017 Solvent Selection Valve Lift up solvent filters in solvent reservoirs to avoid Remove tubings of the solvent selection valve. leakages. Open the purge valve. Power off the system.
  • Page 95 Maintenance Replace the Solvent Selection Valve (G7161B) Connect the cable of the new solvent selection valve. Install the solvent selection valve (1., 2.) and fix it with the screws (3.). Next Steps: 10 Connect tubings to the solvent selection valve. 11 Make sure all capillary and tubing connections are reconnected and tight.

  • Page 96: Replace The Solvent Mixer

    Maintenance Replace the Solvent Mixer Replace the Solvent Mixer Parts required Description G1312-87330 Mixer Lift up solvent filters in solvent reservoirs to avoid Remove the capillaries from the solvent mixer. leakages. Open the purge valve. Power off the system. Open the doors. Remove the solvent mixer.

  • Page 97: Replace The Seal Wash Pump

    Maintenance Replace the Seal Wash Pump Replace the Seal Wash Pump When In case of wear of the seal wash pump Parts required Description 5065-4445 Peristaltic pump with Pharmed tubing 5065-9978 Tubing, 1 mm i.d., 3 mm o.d., silicone, 5 m Lift up solvent filters in solvent reservoirs to avoid Remove the flow connections from and to the seal leakages.
  • Page 98 Maintenance Replace the Seal Wash Pump Insert the pump clips to the holes in the pump housing. Fix the seal wash tubings to the peristaltic pump inlet and from the peristaltic pump outlet to the primary pump head inlet. Next Steps: 10 Make sure all capillary and tubing connections are reconnected and tight.

  • Page 99: Replace The Seal Wash Sensor

    Maintenance Replace the Seal Wash Sensor Replace the Seal Wash Sensor Parts required Description 5067-5950 Seal Wash Sensor Assembly Lift up solvent filters in solvent reservoirs to avoid Remove the tubing of the seal wash sensor. leakages. Open the purge valve. Power off the system.
  • Page 100 Maintenance Replace the Seal Wash Sensor 10 Reconnect and install the seal wash sensor. 11 Connect the tubing of the seal wash sensor. Next Steps: 12 Make sure all capillary and tubing connections are reconnected and tight. 13 Insert solvent filters into solvent reservoirs. 14 Power on the system.

  • Page 101: Replace The Purge Valve

    Maintenance Replace the Purge Valve Replace the Purge Valve When In case of problems with the purge valve Tools required Description 8710-0510 Open-end wrench 1/4 — 5/16 inch 8710-1924 Open-end wrench 14 mm Parts required Description G7161-60300 Purge Valve Lift up solvent filters in solvent reservoirs to avoid Disconnect the waste tube from the purge valve.
  • Page 102 Maintenance Replace the Purge Valve Using a 14 mm wrench unscrew the purge valve and C AU T I O N remove it. Damage to the purge valve  Do not lift the pump using the purge valve as a handle, it might get leaky.

  • Page 103: Replace The Purge Valve Adapter And The Filter

    Maintenance Replace the Purge Valve Adapter and the Filter Replace the Purge Valve Adapter and the Filter When In case of problems with the purge valve Parts required Description G7161-60300 Purge Valve Purge Valve Adapter Lift up solvent filters in solvent reservoirs to avoid Disconnect the waste tube from the purge valve.
  • Page 104 Maintenance Replace the Purge Valve Adapter and the Filter Unscrew and remove the purge valve adapter by pulling Replace the filter of the purge valve adapter. it to the front. Filter 10 Install the new purge valve adapter with the screws. 11 Install all flow connections.
  • Page 105 Maintenance Replace the Purge Valve Adapter and the Filter 13 Reconnect the waste tube to the purge valve. C AU T I O N Damage to the purge valve  Do not lift the pump using the purge valve as a handle, it might get leaky.

  • Page 106: Replace The Inlet Valve Cartridge

    Maintenance Replace the Inlet Valve Cartridge Replace the Inlet Valve Cartridge When If Inlet valve is defective. Tools required Description Wrench, 17 mm Parts required Description 5067-6642 Check valves, 50 mL head G7161-60052 Check valves, 200 mL head Lift up solvent filters in solvent reservoirs to avoid Unscrew the tubing at the inlet valve.
  • Page 107 Maintenance Replace the Inlet Valve Cartridge Attach the inlet tubing at the inlet valve. Next Steps: Make sure all capillary and tubing connections are reconnected and tight. 10 Insert solvent filters into solvent reservoirs. 11 Power on the system. 12 Purge the system. 13 Close the doors.

  • Page 108: Release A Stuck Inlet Valve

    Maintenance Release a Stuck Inlet Valve Release a Stuck Inlet Valve When If inlet valve is stuck, or if pump is not generating pressure after being turned off for an extended period of time. Before the system is turned off for an extended period of time, it should be NOTE flushed with at least 10 % isopropanol to prevent inlet valves from getting stuck.
  • Page 109 Maintenance Release a Stuck Inlet Valve Reconnect tubing to inlet valve. Push solvent with syringe until it comes out at the top of the High Pressure Filter Assembly. 10 Detach the syringe and reconnect the tubing. 11 Reinstall the capillary connection to the High Pressure Filter Assembly.

  • Page 110: Replace The Outlet Valve

    Maintenance Replace the Outlet Valve Replace the Outlet Valve When If Outlet valve is defective. Parts required Description 5067-6642 Check valves, 50 mL head G7161-60052 Check valves, 200 mL head Lift up solvent filters in solvent reservoirs to avoid Remove all tubings and capillaries from the pump head leakages.
  • Page 111 Maintenance Replace the Outlet Valve Reconnect all hydraulic connections. Next Steps: Make sure all capillary and tubing connections are reconnected and tight. 10 Insert solvent filters into solvent reservoirs. 11 Power on the system. 12 Purge the system. 13 Close the doors. Preparative Binary Pumps User Manual...

  • Page 112: Remove The Pump Head Assembly

    Maintenance Remove the Pump Head Assembly Remove the Pump Head Assembly One pump head assembly consists of two pump heads, which are removed one at a time. This procedure describes the replacement of the left pump head assembly (channel A). Similarly, the right pump head assembly (channel B) can be replaced. Tools required Description G7120-68708...

  • Page 113: Pump Head Maintenance

    Maintenance Pump Head Maintenance Pump Head Maintenance Disassemble Pump Heads Seals must be exchanged and pistons must be cleaned in both pump head NOTE assemblies. Remove the pump head screw from the back of the Open the pump head and remove the piston housing pump head.
  • Page 114 Maintenance Pump Head Maintenance Using the steel side of the insert tool, remove the wash Using the steel side of the insert tool, remove the pump seal from the support ring. seal from the pump head assembly. Next Steps: Clean the piston with abrasive paper. Rinse the pump head parts and the piston with isopropanol.

  • Page 115: Assemble Pump Heads

    Maintenance Pump Head Maintenance Assemble Pump Heads Seals must be exchanged in all pump heads. NOTE Lubricate all pump head parts and seals with Using the plastic side of the insert tool, insert a new seal isopropanol. into the pump head. Using the plastic side of the insert tool, insert the new Place the seal holder onto the pump housing.
  • Page 116 Maintenance Pump Head Maintenance Tighten the pump head screw. Preparative Binary Pumps User Manual...

  • Page 117: Install The Pump Head Assembly

    Maintenance Install the Pump Head Assembly Install the Pump Head Assembly Tools required Description G7120-68708 HPLC System Tool Kit-Infinity-II G4220-20013 4 mm hex bit G4220-20015 Adapter ¼ in square to hex Bring the pump drive to the maintenance position using Mount the pump head to the module.
  • Page 118 Maintenance Install the Pump Head Assembly Next Steps: Make sure all capillary and tubing connections are reconnected and tight. Insert solvent filters into solvent reservoirs. Power on the system. Purge the system. Close the doors. 10 Perform a Pump Leak Rate Test. Preparative Binary Pumps User Manual...

  • Page 119: Replace The Module Firmware

    Maintenance Replace the Module Firmware Replace the Module Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary •...

  • Page 120: Prepare The Pump Module For Transport

    Maintenance Prepare the Pump Module for Transport Prepare the Pump Module for Transport When If the module shall be transported or shipped. Preparations Flush both solvent channels with isopropanol. Heavy weight WAR N IN G The module is heavy. Carry the module at least with 2 people. ...
  • Page 121 Maintenance Prepare the Pump Module for Transport 6 Carefully insert the Protective Foam to the front part of the instrument. Do not damage any tubing or capillary connections. 7 Close the front cover. 8 For transport or shipment, put the module and accessory kit to the original shipment box.

  • Page 122: Parts And Materials For Maintenance

    Parts and Materials for Maintenance Overview of Maintenance Parts (G7161A) Overview of Maintenance Parts (G7161B) Flow Connections (G7161A) Flow Connections (G7161B) Purge Valve Pump Heads Pump Head Assembly Parts Accessory Kit HPLC System Tool Kit This chapter provides information on parts for maintenance. Preparative Binary Pumps User Manual...

  • Page 123: Overview Of Maintenance Parts (G7161A)

    Parts and Materials for Maintenance Overview of Maintenance Parts (G7161A) Overview of Maintenance Parts (G7161A) Figure 9 Maintenance parts (G7161A) Item Description G7161-20019 Manifold G7161-60023 Pump Head Kit 50 mL G1312-87330 Mixer G7161-60300 Purge Valve 5067-1527 Pressure sensor 5065-4445 Peristaltic pump with Pharmed tubing 5067-6651 Y-connector assembly 5023-2626...

  • Page 124: Overview Of Maintenance Parts (G7161B)

    Parts and Materials for Maintenance Overview of Maintenance Parts (G7161B) Overview of Maintenance Parts (G7161B) Figure 10 Maintenance parts (G7161B) Item Description G7161-20019 Manifold G7161-60023 Pump Head Kit 50 mL G7161-60021 Pump Head Kit 200 mL G1312-87330 Mixer G7161-60300 Purge Valve 5067-1527 Pressure sensor 5065-4445...

  • Page 125: Flow Connections (G7161A)

    Parts and Materials for Maintenance Flow Connections (G7161A) Flow Connections (G7161A) Y-connector Seal wash pump from solvent reservoir from seal wash to waste solvent reservoir Pump head channel B left Pump head channel B right Manifold Pressure sensor Pump head channel A left to sampler Pump head channel A right Mixer...

  • Page 126: Flow Connections (G7161B)

    Parts and Materials for Maintenance Flow Connections (G7161B) Flow Connections (G7161B) to sampler Solvent selection valve Seal wash sensor Seal wash pump Mixer to waste from seal wash solvent reservoir Pump head channel B left Pump head channel B right Manifold Pressure sensor Pump head channel A left...

  • Page 127: Purge Valve

    Parts and Materials for Maintenance Purge Valve Purge Valve Item Description G7161-60300 Purge Valve Filter purge adapter G7111-60061 Purge valve 5022-2192 Filter frit 2 µm G1361-44601 Filter screw Preparative Binary Pumps User Manual...

  • Page 128: Pump Heads

    Parts and Materials for Maintenance Pump Heads Pump Heads Pump Head Assembly Parts Preparative Binary Pumps User Manual...
  • Page 129 Parts and Materials for Maintenance Pump Heads Assembly parts for 50 mL pump head: Item Description G7161-20083 Inlet Check Valve Holder 5067-6642 Check valves, 50 mL head G1361-25202 Valve Adapter long out 5067-4299 High pressure PE Seals 0905-1994 O-ring Backup assembly 5067-6587 High pressure PE Wash Seals G7161-60005...
  • Page 130 Parts and Materials for Maintenance Pump Heads Item Description 5188-8030 Tag Reader Preparative Binary Pumps User Manual...

  • Page 131: Accessory Kit

    Parts and Materials for Maintenance Accessory Kit Accessory Kit Description 5043-1013 Tubing Clip 5181-1519 CAN cable, Agilent module to module, 1 m 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) 5500-1155 Tube Connector, 90 degree, ID 6.4 5500-1169 Y Tube Connector ID 6.4 5043-1372...

  • Page 132: Hplc System Tool Kit

    Parts and Materials for Maintenance HPLC System Tool Kit HPLC System Tool Kit 5023-2504 (Hex Driver SW-4 slitted) 5023-3138 5023-2503 (Hex Driver (Reversible Screwdriver) SW-5 slitted) 5023-2502 (Hex Driver SW-6, 35/ ¼” slitted) 5023-3088 8710-1924 8710-2409 8710-0510 8710-0510 5023-2500 8710-1534 8710-2394 (Hex Key 9/64“, 15cm) Box with:...

  • Page 133: Identifying Cables

    Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables Agilent Module to PC This chapter provides information on cables used with the modules. Preparative Binary Pumps User Manual...

  • Page 134: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter 01046-60105 Analog cable (BNC to general purpose, spade lugs)
  • Page 135 Identifying Cables Cable Overview LAN cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connec- tion) RS-232 cables (not for Description FUSION board) RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female).

  • Page 136: Analog Cables

    Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
  • Page 137 Identifying Cables Analog Cables Agilent Module to BNC Connector p/n 8120-1840 Pin BNC Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog +...

  • Page 138: Remote Cables

    Identifying Cables Remote Cables Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male - male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 Color code Enhanced...
  • Page 139 Identifying Cables Remote Cables • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding...
  • Page 140 Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Preparative Binary Pumps User Manual...
  • Page 141 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue...

  • Page 142: Can/Lan Cables

    Identifying Cables CAN/LAN Cables CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203...

  • Page 143: Agilent Module To Pc

    Identifying Cables Agilent Module to PC Agilent Module to PC Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.

  • Page 144: Usb

    Identifying Cables To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Preparative Binary Pumps User Manual...

  • Page 145: Hardware Information

    Hardware Information Firmware Description Electrical Connections Serial Number Information Rear view of the module Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch Early Maintenance Feedback Instrument Layout This chapter describes the module in more detail on hardware and electronics. Preparative Binary Pumps User Manual...

  • Page 146: Firmware Description

    Hardware Information Firmware Description Firmware Description The firmware of the instrument consists of two independent sections: • a non-instrument specific section, called resident system • an instrument specific section, called main system Resident System This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules.
  • Page 147 Hardware Information Firmware Description Firmware Updates Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used). Required tools, firmware and documentation are available from the Agilent web: http://www.agilent.com/en-us/firmwareDownload?whid=69761 The file naming conventions are: PPPP_RVVV_XXX.dlb, where •...
  • Page 148 Hardware Information Firmware Description Some modules are limited in downgrading due to their main board version or NOTE their initial firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware revision B.01.02 or to a A.xx.xx. Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in specific control software environments.

  • Page 149: Electrical Connections

    There are no externally accessible fuses, because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations.

  • Page 150: Serial Number Information

    Hardware Information Electrical Connections Serial Number Information The serial number information on the instrument labels provide the following information: CCXZZ00000 Format Country of manufacturing • DE = Germany • JP = Japan • CN = China Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module)

  • Page 151: Interfaces

    Hardware Information Interfaces Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 6 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Pumps G7104A/C G7110B G7111A/B, G5654A G7112B G7120A G7161A/B Samplers...
  • Page 152 Hardware Information Interfaces Table 6 Agilent InfinityLab LC Series Interfaces Module RS-232 Analog APG (A) Special (on-board) / ERI (E) Fraction Collectors G7158B G7159B G7166A Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with...

  • Page 153: Overview Interfaces

    Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 154 Hardware Information Interfaces To provide maximum safety within a distributed analysis system, one line is dedicated to SHUT DOWN the system’s critical parts in case any module detects a serious problem. To detect whether all participating modules are switched on or properly powered, one line is defined to summarize the POWER ON state of all connected modules.

  • Page 155: Eri (Enhanced Remote Interface)

    ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the FUSION core electronics use ERI. This interface is already used in the Agilent Universal...
  • Page 156 Hardware Information Interfaces Enhanced Remote IO 1 (START REQUEST) IO 2 (STOP) IO 3 (READY) IO 4 (POWER ON) IO 5 (NOT USED) IO 6 (SHUT DOWN) IO 7 (START) IO 8 (PREPARE) 1 wire DATA DGND +5 V ERI out PGND PGND +24 V ERI out...

  • Page 157: Usb (Universal Serial Bus)

    Hardware Information Interfaces 5V Distribution (Future Use) • Available directly after turn on oft the hosting module (assures that certain base functionality of the device can be detected by firmware). • For digital circuits or similar. • Provided 500 mA maximum. •...

  • Page 158: Setting The 6-Bit Configuration Switch

    Hardware Information Setting the 6-bit Configuration Switch Setting the 6-bit Configuration Switch The 6-bit configuration switch is located at the rear of the module with FUSION electronics. Switch settings provide configuration parameters for LAN and instrument specific initialization procedures. All modules with FUSION electronics: •...
  • Page 159 Hardware Information Setting the 6-bit Configuration Switch Table 8 6-bit Configuration Switch Mode Function/Setting Switch 1 Switch 2 Switch 3 Switch 4 Switch 5 Switch 6 n.a. LAN Init Mode n.a. n.a. Use Default IP Address Use Stored IP Address Use DHCP to request IP Address Test System...

  • Page 160: Early Maintenance Feedback

    Hardware Information Early Maintenance Feedback Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.

  • Page 161: Instrument Layout

    Hardware Information Instrument Layout Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E-PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.

  • Page 162: Lan Configuration

    LAN Configuration What You Have to Do First TCP/IP parameter configuration Configuration Switches Initialization Mode Selection Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) Setup (DHCP) Manual Configuration With Telnet This chapter provides information on connecting the module to the Agilent ChemStation PC.

  • Page 163: What You Have To Do First

    LAN Configuration What You Have to Do First What You Have to Do First The module has an on- board LAN communication interface. This chapter is generic and may show figures that differ from your module. The NOTE functionality is the same. 1 Note the MAC (Media Access Control) address for further reference.

  • Page 164: Tcp/Ip Parameter Configuration

    LAN Configuration TCP/IP parameter configuration TCP/IP parameter configuration To operate properly in a network environment, the LAN interface must be configured with valid TCP/IP network parameters. These parameters are: • IP address • Subnet Mask • Default Gateway The TCP/IP parameters can be configured by the following methods: •...

  • Page 165: Configuration Switches

    LAN Configuration Configuration Switches Configuration Switches The configuration switch can be accessed at the rear of the module. Configuration switch Figure 19 Location of Configuration switch (example shows a G7114A/B VWD) The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF.

  • Page 166: Initialization Mode Selection

    LAN Configuration Initialization Mode Selection Initialization Mode Selection The following initialization (init) modes are selectable: Table 9 Initialization Mode Switches Init Mode Use Default IP Address Use Stored IP Address Use DHCP Note: The setting ‘0’ (down) is essential. Default IP address for LAN is 192.168.254.11. DHCP address is the module’s LAN MAC address.
  • Page 167 LAN Configuration Initialization Mode Selection Using Default When Using Default is selected, the factory default parameters are taken instead. These parameters enable a TCP/IP connection to the LAN interface without further configuration, see Table 10 on page 167. Default Active Parameter Parameter Figure 21...

  • Page 168: Dynamic Host Configuration Protocol (Dhcp)

    LAN Configuration Dynamic Host Configuration Protocol (DHCP) Dynamic Host Configuration Protocol (DHCP) General Information (DHCP) The Dynamic Host Configuration Protocol (DHCP) is an auto configuration protocol used on IP networks. The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware.

  • Page 169: Setup (Dhcp)

    LAN Configuration Dynamic Host Configuration Protocol (DHCP) Setup (DHCP) The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware. All modules should use latest firmware from the same set. 1 Note the MAC address of the LAN interface (provided with G1369C LAN Interface Card or Main Board).
  • Page 170 LAN Configuration Dynamic Host Configuration Protocol (DHCP) 2 Set the Configuration Switch to DHCP either on the G1369C LAN Interface Card or the main board of above mentioned modules. Table 11 G1369C LAN Interface Card (configuration switch on the card) SW 4 SW 5 SW 6...

  • Page 171: Manual Configuration

    LAN Configuration Manual Configuration Manual Configuration Manual configuration only alters the set of parameters stored in the non-volatile memory of the module. It never affects the currently active parameters. Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing it.

  • Page 172: With Telnet

    LAN Configuration Manual Configuration With Telnet Whenever a TCP/IP connection to the module is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. 1 Open the system (DOS) prompt window by clicking on Windows START button and select “Run...”.
  • Page 173 LAN Configuration Manual Configuration Table 13 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip <x.x.x.x> sets new ip address sm <x.x.x.x> sets new subnet mask gw <x.x.x.x> sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style: •...
  • Page 174 LAN Configuration Manual Configuration 6 Change the IP address (in this example 192.168.254.12) and type “/” to list current settings. change of IP setting to Initialization mode is Using Stored active TCP/IP settings stored TCP/IP settings in non-volatile memory connected to PC with controller software (e.g. Agilent ChemStation), here not connected Figure 29 Telnet - Change IP settings...

  • Page 175: Appendix

    Do Not Modify the Instrument In Case of Damage Solvents Safety Symbols Waste Electrical and Electronic Equipment (WEEE) Directive Radio Interference Sound Emission Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Preparative Binary Pumps User Manual...

  • Page 176: General Safety Information

    Appendix General Safety Information General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.

  • Page 177: Before Applying Power

    Verify that the voltage range and frequency of your power distribution  matches to the power specification of the individual instrument. Never use cables other than the ones supplied by Agilent Technologies  to ensure proper functionality and compliance with safety or EMC regulations.

  • Page 178: Ground Solvent Lines

    Electrical shock, fire, explosion Never use solvent guiding tubes, capillaries, and fittings other than the  ones supplied by Agilent Technologies for use in preparative systems. Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WAR N IN G...

  • Page 179: In Case Of Damage

    Appendix General Safety Information In Case of Damage Damage to the module WAR N IN G Personal injury (for example electrical shock, intoxication) Instruments that appear damaged or defective should be made  inoperative and secured against unintended operation until they can be repaired by qualified service personnel.

  • Page 180: Solvents

    Appendix General Safety Information Solvents Toxic, flammable and hazardous solvents, samples and reagents WAR N IN G The handling of solvents, samples and reagents can hold health and safety risks. When working with these substances observe appropriate safety  procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.

  • Page 181: Safety Symbols

    Appendix General Safety Information Safety Symbols Table 14 Symbols The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages.
  • Page 182 Appendix General Safety Information Table 14 Symbols Magnetic field Magnets produce a far-reaching, strong magnetic field. They could damage TVs and laptops, computer hard drives, credit and ATM cards, data storage media, mechanical watches, hearing aids and speakers. Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields.

  • Page 183: Waste Electrical And Electronic Equipment (Weee) Directive

    Appendix Waste Electrical and Electronic Equipment (WEEE) Directive Waste Electrical and Electronic Equipment (WEEE) Directive This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste. Do not dispose of in domestic household waste NOTE To return unwanted products, contact your local Agilent office, or see...

  • Page 184: Radio Interference

    Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.

  • Page 185: Sound Emission

    Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB. •...

  • Page 186: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Preparative Binary Pumps User Manual...
  • Page 187 Index Index extra-column volume delay volume description fan failed DHCP firmware general information description setup Agilent Lab Advisor software main system dimensions resident system Agilent Lab Advisor disassembling update tool Agilent pump head updates 147, on internet upgrade/downgrade ambient non-operating fittings temperature electrical connections...
  • Page 188 Index internet motor-drive power radio interference remote (ERI) non-operating altitude remote cable non-operating temperature cables configuration switche repairs manual configuration with replacing firmware telnet replace operating Altitude manual configuration inlet valve operating temperature TCP/IP parameter manifold optimization configuration outlet valve achieving higher using default purge valve adapter...
  • Page 189 Index telnet configuration temperature limit exceeded temperature sensor timeout transport prepare troubleshooting error messages voltage range waste electrical and electronic equipment WEEE directive weight Preparative Binary Pumps User Manual...
  • Page 190 The manual describes the following: • introduction and specifications, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • hardware information, • safety and related information. www.agilent.com © Agilent Technologies Inc. 2017-2019 Published in Germany 10/2019 D0000602 Rev.B...

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