Additional Information; Cautionary Note - Waters ACQUITY UPLC Care And Use Manual

[ CARE AND USE MANUAL ] [ CARE AND USE MANUAL ]
VI. ADDITIONAL INFORMATION
a. Band Spreading Minimization
Waters ACQUITY UPLC System is designed to have a
minimal post column band spreading. If desirable, the mass
spectrometer can be connected either directly or in series
with a UV (PDA) detector. The connecting tubing internal
diameter should be 100 µm or less in order to preserve the
achieved separation. Length of the tubing should also be
kept to a minimum.
Detritylated synthetic oligonucleotide separations are almost
exclusively performed using gradient elution techniques. As
such, the effect of pre-column sample band broadening can be
minimized by allowing the sample to bind to the column before
beginning the actual separation gradient. However, proper
connection from the ACQUITY UPLC and ACQUITY Premier
Oligonucleotide BEH C Column outlet to the detector is
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critical in order to minimize the deleterious effect of p-column
sample band spreading. Use of appropriate internal diameter
tubing (e.g., 0.005 inch PEEK™ tubing for UV detector
applications) is recommended.
Waters, The Science of What's Possible, ACQUITY, UPLC, BEH Technology, MaxPeak, eCord, Empower,
MassLynx, and MassPREP are trademarks of Waters Corporation. PEEK is a trademark of Victrex plc.
All other trademarks are the property of their respective owners.
©2021 Waters Corporation. Produced in the U.S.A. November 2021 715001464 Rev H IH-PDF
VII. CAUTIONARY NOTE
Depending on user's application, these products may be
classified as hazardous following their use and as such are
intended to be used by professional laboratory personnel
trained in the competent handling of such materials.
Responsibility for the safe use and disposal of products rest
entirely with the purchaser and user. The Safety Data Sheet
(SDS) for this product is available at www.waters.com/sds.
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com