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PerkinElmer VICTOR Nivo User Manual

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VICTOR N
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User Manual
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Summary of Contents for PerkinElmer VICTOR Nivo

  • Page 1 ® VICTOR N User Manual (English)
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    Contents Safety Safety Symbols Usage of the VICTOR Nivo Environmental Conditions Safety Instructions and Warnings 1.4.1 Electrical Hazard 1.4.2 Hot Surfaces 1.4.3 Spillage of Liquids 1.4.4 Mechanical Hazards 1.4.5 1.4.6 UV Radiation 1.4.7 Dangerous Substances Laser Radiation Instrument Type Labels...
  • Page 4 6.2.4 Installing Tubes Between Gas Tanks and Gas Controller 6.2.5 Installing Tubes Between Gas Controller and VICTOR Nivo reader Setting the Pressure Checking the System and Switching on the Gas Controller 6.4.1 Check Cables and Tubing 6.4.2 Check Basic Settings of Gas Controller Gas Controller Configuration 6.5.1...
  • Page 5 10.3.2 Barcode Scanner 10.3.3 Stacker Speed 10.4 Plate and Lid Compatibility 10.4.1 General 10.4.2 Stacker Index...

  • Page 6: Safety

    Notice Failure to observe may result in invalid measurement results or damage of the instrument Notice Useful hint or explanation Biohazard Electrical voltage Hot surface Flammable material Ultraviolet (UV) radiation Corrosive material Toxic material Laser radiation VICTOR Nivo – User Manual...

  • Page 7: Usage Of The Victor Nivo

    PerkinElmer service personnel. For research use only. Not for clinical diagnostic use. The VICTOR Nivo is an automated multimode plate reader for use in biological and pre-clinical research only. The VICTOR Nivo is particularly suited for cellular and biochemical research applications.

  • Page 8: Environmental Conditions

    Pollution degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation. Both of these affect the dimensioning of the electrical insulation within the instrument. VICTOR Nivo – User Manual...

  • Page 9: Safety Instructions And Warnings

    1 Safety Safety Instructions and Warnings The VICTOR Nivo system and its optional components must be installed and used according to the instructions given by the manufacturer. The service must be done by properly trained personnel authorized by the manufacturer. The violation of these instructions may result in warranty invalidation or malfunction of the equipment.

  • Page 10: Spillage Of Liquids

    It is recommended to set the measuring times for acid samples only to the absolutely necessary times. Do not leave the plate in the instrument after the measurement. VICTOR Nivo – User Manual...

  • Page 11: Mechanical Hazards

    Do not touch the pins and be very careful when working near the gripper. Warning! Do not attempt to stop or limit the stacker movement while the system is powered on. Doing so might injure the user or damage the system. VICTOR Nivo – User Manual...

  • Page 12: Gas

    Keep the plate door of the VICTOR Nivo closed to avoid the escape of gas. Gas supply must be switched off during downtime of the instrument.

  • Page 13: Laser Radiation

    The cover of the instrument should not be removed or altered in any way. Doing so could lead to hazardous laser radiation exposure. The VICTOR Nivo Alpha reader’s plate loading door and upper hatch are equipped with safety sensors which immediately prevent the laser operation if any of the hatches is opened during a measurement.

  • Page 14: Instrument Type Labels

    1 Safety Instrument Type Labels VICTOR Nivo Reader Dispenser (optional) Stacker (optional) VICTOR Nivo – User Manual...

  • Page 15: Introduction

    (optional) Measurement Technologies The VICTOR Nivo Multimode Plate Reader can be used to quantitatively measure light emitting or absorbing substances both from top and bottom side of the plate, making it suitable for several different assay types including kinetic, cellular, enzymatic and autoimmune assays.
  • Page 16 2 Introduction Wi-Fi router USB stick (in VICTOR Nivo design) containing: PDF manuals (Installation Guide, Quick Start Guide, Release Notes, User Manual, Software Manual, Site Readiness document) VICTOR Nivo Filter List Printed manuals (User Manual, Installation Guide, Quick Start Guide) Optional:...

  • Page 17: Reader Installation

    These settings are also applied to the VICTOR Nivo and the control software. If you change any of the time settings on the control device, you have to switch off the VICTOR Nivo, unplug it from the mains and then restart it to have the time re-synchronized. ®...

  • Page 18: Software Requirements

    The reader can be operated using any device having either Microsoft Windows, Mac OS, iOS or Android installed. The VICTOR Nivo comes with a pre-installed user interface software that is used to operate the reader and export the results. The user interface is accessed through a web browser.

  • Page 19: Reader Positioning

    3 Reader Installation Notice Please check regularly for software updates on the PerkinElmer website: http://www.perkinelmer.com/lab-products-and-services/resources/software- downloads.html Click on “Multimode Plate Reader” to go to the software downloads for the VICTOR Nivo. See also chapter 3.7 “Software Update”, page 27. Reader Positioning Place the reader on a flat and even table surface free of dust, liquids or acidic vapors.

  • Page 20: Turning The Reader On And Off

    2. Connect the Ethernet port on the rear of the instrument to the Ethernet port of your control device (e.g. laptop) using the LAN network cable (D). VICTOR Nivo – User Manual...
  • Page 21 IP address configuration of the control PC Configure the network settings of your control device to match the IP address of the VICTOR Nivo instrument. Please note that these instructions are shown for a Windows 10 operating system. 1. Open Control Panel > Network and Internet > Network and Sharing Centre.
  • Page 22 3 Reader Installation 3. Click Properties. 4. Select Internet Protocol Version 4 and click Properties. Select Use the following IP address. Enter IP address 192.168.0.100 and subnet mask 255.255.255.0 VICTOR Nivo – User Manual...

  • Page 23: Installation With Wi-Fi Router

    5. Click OK. Connecting to the VICTOR Nivo 1. Switch on the VICTOR Nivo using the power switch. 2. Open your web browser. Type http://192.168.0.2 into the address bar and press the Enter key. The user interface appears and the instrument is initialized automatically.
  • Page 24 2. Switch on your control device with wireless network capability (e.g. a laptop). If you are using the optional laptop or tablet from PerkinElmer and start it for the first time, the Windows 10 first start routine will appear. Configure user name, password, language settings etc.

  • Page 25: Making Reader Ip Address A Trusted Site

    1. Open Control Panel > Network and Internet > Internet Options. 2. Select the Security tab. 3. Select the zone Trusted sites and click the Sites button. VICTOR Nivo – User Manual...
  • Page 26 4. If the check box Require server verification (https:) for all sites in this zone is selected, clear the check box. 5. Type http://192.168.0.2 into the field Add this website to the zone and click Add. The reader’s IP address is added to the trusted sites. VICTOR Nivo – User Manual...

  • Page 27: Software Update

    2. Copy the setup file SetupVICTORNivo5-0-X.exe to the PC connected to the VICTOR Nivo. 3. Make sure that the VICTOR Nivo is switched on and connected to the PC (via LAN or WiFi). 4. Close the user interface (web browser).
  • Page 28 3 Reader Installation Notice Please check regularly for software updates on the PerkinElmer website: http://www.perkinelmer.com/lab-products-and-services/resources/software- downloads.html Click on “Multimode Plate Reader” to go to the software downloads for the VICTOR Nivo. VICTOR Nivo – User Manual...

  • Page 29: Initializing The Reader

    Enhanced Security mode: Select a user account from the drop down menu, enter the corresponding password in and click OK. See also VICTOR Nivo Software Manual, chapters “Siging In and Out” and “User Account Management”. VICTOR Nivo – User Manual...
  • Page 30 Keep away from the working area of the stacker during initialization. When the initialization is finished, the PROTOCOLS page will appear on the screen. See VICTOR Nivo Software Manual, chapter “User Interface Overview (PROTOCOLS)” for an introduction to the user interface.

  • Page 31: Dispenser Module (Optional)

    3. Reagent bottles 4. Dispensing tubes from pump unit to dispenser head 5. Input tubes from reagent bottle to pump unit 6. Control buttons 7. Dispenser head (in measurement position) 8. Dispenser head (removed for priming/washing) VICTOR Nivo – User Manual...

  • Page 32: Prime

    3. Place the input tube of pump 1 into the reagent vessel which contains the liquid to be dispensed. Click Wash/Prime of pump 1 on the left side to prime the pump and the tubing. If necessary, click this button again until the liquid is VICTOR Nivo – User Manual...

  • Page 33: Wash

    2. Remove the dispenser tip from the reader and place it into a waste container. 3. Take the input tube of the corresponding pump out of the reagent bottle (to prime with air) or put it into a vessel with water/ethanol for washing. VICTOR Nivo – User Manual...

  • Page 34: Empty Syringe

    The WASH/PRIME button will start the washing of the pump. Priming does not start if the dispensing tip is inside the reader. Empty Syringe After dispensing, the remaining liquids can be returned to the reagent bottle by clicking the Empty syringe button. VICTOR Nivo – User Manual...
  • Page 35 Emptying the syringes can also be performed by using the buttons located at the dispenser unit. The PUMP SEL button is used to select the pump. Green light indicates the selected pump. The EMPTY button will start emptying the syringe. VICTOR Nivo – User Manual...

  • Page 36: Gas Control (Optional)

    6 Gas Control (Optional) Gas Control (Optional) An external gas control option is available for VICTOR Nivo readers. This digital controller allows to control (and log) the CO and O concentrations by infusing pure CO and pure N . The CO concentration is adjustable in the range of 0- 20 %, the O...

  • Page 37: Installation

    Keep the plate door of the VICTOR Nivo closed to avoid the escape of gas. Gas supply must be switched off during downtime of the instrument.

  • Page 38: Supplied Equipment

    “N Input” at the gas controller disconnected. 5. 12V DC power supply 6. VICTOR Nivo reader (rear panel) 7. Gas connectors (tubing to be connected using L-shape tubing adapters, item 5 on page 40) 8. N tank + pressure regulator VICTOR Nivo –...
  • Page 39 Use TUBE AA to connect the “Gas Output” of the gas controller to the lower connector of the VICTOR Nivo reader (use L-shape tubing adapter, see item 5). 3. TUBE AB (x1), 2 m long, blue labeled with PTFE filter Use TUBE AB to connect the “Gas sensors Input”...

  • Page 40: Gas Controller (Rear View)

    5. L-shape tubing adapter (x2) Use the adapter to connect TUBE AA and AB to the threaded female gas connectors of the VICTOR Nivo reader. Danger of Gas Leakage! Do not expose the tubing to any mechanical or thermal stress.

  • Page 41: Installing Tubes Between Gas Tanks And Gas Controller

    In-Out orientation (see figure below). 5. Use TUBE A to connect the gas output of the pressure gauge with the CO or N Input push-in connector (see figure below) of the gas controller. VICTOR Nivo – User Manual...

  • Page 42: Installing Tubes Between Gas Controller And Victor Nivo Reader

    N tanks (1) to an output pressure of 2.0 bar. 2. Adjust the supplied pressure gauges (2 in the figure above, installed between the CO tank and the gas controller) to an operating pressure of 0.5 bar: VICTOR Nivo – User Manual...
  • Page 43 6 Gas Control (Optional) A. Pull the knob up to release the lock. B. Rotate the knob until the pressure reaches the desired value (clockwise/counter clockwise to increase/decrease the pressure). C. Push the knob down to lock it. VICTOR Nivo – User Manual...

  • Page 44: Checking The System And Switching On The Gas Controller

    2. Turn on the gas controller using the switch on the lower right of the front panel. 6.4.2 Check Basic Settings of Gas Controller The gas controller is delivered with factory preset settings for use with VICTOR Nivo. To use the optimal parameters for your working conditions, please perform the following steps: 1.

  • Page 45: Gas Controller Configuration

    In this case you don’t regulate O levels. The touch screen allows you to switch from standard CO mode to CO mode and back: VICTOR Nivo – User Manual...
  • Page 46 6 Gas Control (Optional) 1. Go to the homepage. 2. Tap on the O2 tab. 3. Tap + until ‘Air’ appears. 4. Tap Set to save. VICTOR Nivo – User Manual...

  • Page 47: Stacker Module (Optional)

    7 Stacker Module (Optional) Stacker Module (Optional) Overview The stacker module is an optional extension for the VICTOR Nivo multimode plate reader. It enables semi-automated microplate handling. The stacker module has to be installed by PerkinElmer Service. 1. Control panel 6.

  • Page 48: Positioning

    Make sure that the local main power matches the power requirements of the external power supply. The power requirements are printed on the power supply. VICTOR Nivo reader: 3. USB-A port (connection to the stacker) 4. LAN/Ethernet (RJ-45, connection the PC) VICTOR Nivo – User Manual...

  • Page 49: Turning The Stacker On And Off

    Keep away from the working area of the stacker during initialization. To turn the entire system off, press and hold down the power button on the VICTOR Nivo reader for at least 3 seconds until the light of the button goes out. VICTOR Nivo – User Manual...

  • Page 50: Safety Features

    If there is a plate stuck in the gripper during a critical failure, do not attempt to remove the plate. Doing so might damage the gripper. For further instructions see VICTOR Nivo Software Manual, chapter “Stacker Error Handling”. VICTOR Nivo – User Manual...

  • Page 51: Operation Via Software

    7 Stacker Module (Optional) Operation via Software The stacker is mainly controlled by the VICTOR Nivo control software. The stacker can be operated either using the functions on the SETTINGS > STACKER page or by protocol operations. If you want to use the reader without stacker, you can remove all stacker-specific elements from the user interface: SETTINGS >...

  • Page 52: Status Leds

    LED ring indicates the stacker status: Stacker is ready for Stacker error operation All LED segments are All LEDs are steady green. steady red (until the user confirms the error) Stacker is initializing / operating All LEDs are blinking green. VICTOR Nivo – User Manual...

  • Page 53: Magazines

    IN = left position, OUT = right position) and slide it to the front until it stops. The IN/OUT LEDs light up green when the magazines have been positioned correctly. 4. Make sure that the upper green movable handles are folded down. VICTOR Nivo – User Manual...

  • Page 54: Transport

    Do not attempt to carry or otherwise transport the system. This may result in the position of the reader being misaligned and the stacker having to be readjusted. This can only be done by PerkinElmer Service. VICTOR Nivo – User Manual...

  • Page 55: Retooling

    8 Retooling Retooling Changing Optical Components See the VICTOR Nivo Software Manual, chapter “OPTICS” for a detailed description of the SETTINGS > OPTICS page of the control software with all functions and the respective user access rights. 8.1.1 Loading a Filter Notice Be careful when loading and unloading filters.

  • Page 56: Unloading A Filter

    (bright background). 8.1.2 Unloading a Filter 1. Select a present filter (indicated by a bright background) from the list. In the example below, the 260/10nm filter is selected: VICTOR Nivo – User Manual...

  • Page 57: Changing A Mirror Slide

    1. Open the SETTINGS > OPTICS page. 2. Click Unload Slide. The lid at the front will open and the mirror slide will be ejected: 3. Remove the mirror slide and replace it with a different slide. VICTOR Nivo – User Manual...
  • Page 58 4. Push the slide in so that all mirrors in the slide are inside the reader. 5. Click the Load Slide button of the slide you want to insert into the reader. The slide will start moving inside the reader. 6. Close the optics loading door. VICTOR Nivo – User Manual...

  • Page 59: Installing New Mirrors In A Mirror Slide

    5 individual ones). If you still have a previous revision (also indicated on the slide’s label) and you are experiencing an issue with this type of mirror slide, please contact your PerkinElmer representative. VICTOR Nivo – User Manual...
  • Page 60 Please wear gloves to avoid finger prints on the mirrors. 1. Place the mirror slide on the table (screw heads are accessible) and loosen all 10 screws using a Phillips P1 screwdriver. 2. Remove the mirror slide (plastic frame). VICTOR Nivo – User Manual...
  • Page 61 6. Update the label on the mirror slide that states name and position of each mirror. 7. Update the corresponding mirror slide in the VICTOR Nivo software. Please keep in mind that only admin users can load/unload mirror slides and create/edit mirrors and mirror slides.

  • Page 62: Maintenance

    Optical components must be kept free of dust and finger prints. Dust can be removed by using canned air intended for optics cleaning or very clean compressed air. Finger prints can be removed by using pure ethanol (see below). VICTOR Nivo – User Manual...

  • Page 63: Changing The Air Filter

    Specification of filter material: 92 mm air filter made of polyurethane foam 45 pores per inch (ppi) PerkinElmer spare part order number: "HH35900006 VICTOR Nivo Air Inlet Filter" Warning! Switch off the instrument and unplug from mains before changing the air filter.

  • Page 64: Dispenser Maintenance

    3. Replace the air filter. 4. Attach the filter frame using the four screws. Dispenser Maintenance Before and after utilization, the pump(s) should be thoroughly washed. For detailed instructions see chapter 5.2 “Wash”, page 33. VICTOR Nivo – User Manual...

  • Page 65: Specifications

    Spare part order number: HH35900006 Z-focus range 0 – 16 mm Shaking modes Linear, orbital, double orbital Number of optical filters Up to 32 Number of dichroic mirrors Up to 5 per slide Instrument connection Ethernet VICTOR Nivo – User Manual...

  • Page 66: Dimensions

    VICTOR Nivo Technologies Alpha F Alpha S Absorbance: Filter-Based (F) or Spectrometer (S) Fluorescence Luminescence Time-resolved Fluorescence Fluorescence Polarization Alpha Included Optional 10.1.4 Performance Property Value ABS OD accuracy <2.0% (OD 2) ABS OD precision <1.0% (OD 2) VICTOR Nivo – User Manual...

  • Page 67: Dispenser (Optional)

    Center wavelength (centroid) 680 ± 3 nm @ 350 mW CW power output 400 mW Wavelength temperature coefficient < 0.3 nm/°C Laser class 10.1.7 Electrical Requirements One individual power socket is required for each system component: VICTOR Nivo – User Manual...
  • Page 68 10.1.8 Property Value Size of internal data 12 GB storage Supported web Recommended and tested: browser Google Chrome (of control device) Microsoft Edge (Chromium) Microsoft Internet Explorer and Microsoft Edge (Legacy) are no longer supported. VICTOR Nivo – User Manual...

  • Page 69: Victor Nivo Test Plate (Optional)

    10 Specifications 10.2 VICTOR Nivo Test Plate (Optional) Battery-operated test plate Order number: HH35000795 VICTOR Nivo Test Plate Kit A. ON / OFF switch B. Status LED Battery status (green, orange, red) C. Battery (1x AAA) D. Photodiode (communication between plate and instrument)

  • Page 70: Stacker Module (Optional)

    See chapter 10.4 “Plate and Lid Compatibility”, page 73. compatibility Measurement modes Unlidded plates, lidded plates (lids can remain or be removed for measurement) Maximum magazine 20 plates (plate stack height must not exceed magazine capacity height) VICTOR Nivo – User Manual...
  • Page 71 Please note that the software supports only the following characters in the barcodes: uppercase letters (A-Z) lowercase letters (a-z) numbers (0-9) special characters - . $ / + % and space max. 20 characters in total VICTOR Nivo – User Manual...
  • Page 72 32:37 20 plates 42:27 00:22 Stacker handling time 00:53 01:21 04:30 Restack time 12:48 Option enabled Option disabled Fluorescence intensity, 96-well plate, 20 ms measurement time Time between two subsequent plates in a stacker measurement VICTOR Nivo – User Manual...
  • Page 73 If the optional stacker is used, the following additional/different requirements apply. Property Value Top surface Min. width: 81.2 mm Minimum plate height 8 mm (without dispenser) (from top surface downwards) 12 mm (with dispenser) Minimum lid height 3.5 mm (from top surface downwards) VICTOR Nivo – User Manual...
  • Page 74 11 Index 11 Index VICTOR Nivo – User Manual...
  • Page 75 Laser safety 13 Electrical requirements 67 Loading a filter 55 Empty syringe (dispenser) 34 Logging in 29 Environmental conditions 8 Maintenance 62 Filter Making reader IP address a trusted site 25 Load 55 Manuals 16 VICTOR Nivo – User Manual...
  • Page 76 Unloading a filter 56 Quick Start Guide 16 Updating the reader software 27 USB stick 16 Reader installation 17 Reader performance 66 VICTOR Nivo Test Plate (specs) 69 Reader positioning 19 Reader specifications 65 Wash (dispenser) 33 Removing dust 62 Web browser 18...
  • Page 78 PerkinElmer Schnackenburgallee 114 22525 Hamburg Germany Internet: www.perkinelmer.com Contact: www.perkinelmer.com/contactus...

This manual is also suitable for:

Hh3500Hh35000700Hh35000800

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