PerkinElmer VICTOR NIVO User Manual
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PerkinElmer VICTOR NIVO User Manual

PerkinElmer VICTOR NIVO User Manual

Multimode detection
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Summary of Contents for PerkinElmer VICTOR NIVO

  • Page 1 ® VICTOR N User Manual...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    Contents Safety Safety Symbols Usage of the VICTOR Nivo Safety Instructions and Warnings Laser Radiation Introduction Measurement Technologies Contents of Delivery Reader Installation Hardware Requirements 3.1.1 Computer Hardware 3.1.2 Control Device Power Options Software Requirements Reader Positioning Power and Communication Connections...
  • Page 4 Measurement Protocols Protocols on the VICTOR Nivo User Interface Overview (PROTOCOLS page) Protocol Handling 6.3.1 Creating a New Protocol 6.3.2 Copying a Protocol 6.3.3 Editing a Protocol 6.3.4 Deleting a Protocol 6.3.5 Exporting a Protocol 6.3.6 Importing a Protocol 6.3.7...
  • Page 5 Shaker Overview Shaking via Main Menu Shaking Within the Protocol Handling Optical Components 10.1 Filters 10.1.1 Loading a Filter 10.1.2 Unloading a Filter 10.1.3 Creating a New Filter 10.2 Mirrors 10.2.1 Creating a New Mirror 10.3 Mirror Slides 10.3.1 Changing a Mirror Slide 10.3.2 Editing a Mirror Slide Dispenser Option...
  • Page 6 14.2.5 Installing Tubes Between Gas Controller and VICTOR Nivo reader 14.3 Setting the Pressure 14.4 Checking the System and Switching on the Gas Controller 14.4.1 Check Cables and Tubing 14.4.2 Check Basic Settings of Gas Controller 14.5 Gas Controller Configuration 14.5.1...

  • Page 7: Safety

    1 Safety Safety VICTOR Nivo – User Manual...

  • Page 8: Safety Symbols

    Notice Failure to observe may result in invalid measurement results or damage of the instrument Notice Useful hint or explanation Biohazard Electrical voltage Hot surface Flammable material Ultraviolet (UV) radiation Corrosive material Toxic material Laser radiation VICTOR Nivo – User Manual...

  • Page 9: Usage Of The Victor Nivo

    PerkinElmer service personnel. For research use only. Not for clinical diagnostic use. The VICTOR Nivo is an automated multimode plate reader for use in biological and pre-clinical research only. The VICTOR Nivo is particularly suited for cellular and biochemical research applications.

  • Page 10: Safety Instructions And Warnings

    The VICTOR Nivo Multimode Plate Reader may only be operated in a laboratory environment and by qualified laboratory staff who have been specially trained and are familiar with the contents of this manual. The user...
  • Page 11 Avoid overfilling of the wells. Shaking of a plate with overfilled wells or at too high speed can cause spilling and contamination of the reader. VICTOR Nivo – User Manual...
  • Page 12 Keep the plate door of the VICTOR Nivo closed to avoid the escape of gas. Gas supply must be switched off during downtime of the instrument.

  • Page 13: Laser Radiation

    Doing so could lead to hazardous laser radiation exposure. The VICTOR Nivo Alpha reader’s plate loading door and upper hatch are equipped with safety sensors which immediately prevent the laser operation if any of the hatches is opened during a measurement.

  • Page 14: Introduction

    2 Introduction Introduction VICTOR Nivo – User Manual...

  • Page 15: Measurement Technologies

    2 Introduction Measurement Technologies The VICTOR Nivo Multimode Plate Reader can be used to quantitatively measure light emitting or absorbing substances both from top and bottom side of the plate, making it suitable for several different assay types including kinetic, cellular, enzymatic and autoimmune assays.

  • Page 16: Reader Installation

    3 Reader Installation Reader Installation VICTOR Nivo – User Manual...

  • Page 17: Hardware Requirements

    These settings are also applied to the VICTOR Nivo and the control software. If you change any of the time settings on the control device, you have to switch off the VICTOR Nivo, unplug it from the mains and then restart it to have the time re-synchronized. ®...

  • Page 18: Software Requirements

    The reader can be operated using any device having either Microsoft Windows, Mac OS, iOS or Android installed. The VICTOR Nivo comes with a pre-installed user interface software that is used to operate the reader and export the results. The user interface is accessed through a web browser.

  • Page 19: Power And Communication Connections

    Turning the Reader On and Off To turn the reader on, briefly press the power button. To turn the reader off, press and hold down the power button for 3 seconds until the light of the button goes out. VICTOR Nivo – User Manual...

  • Page 20: Computer Configuration And Connecting The Reader

    IP address configuration of the control PC Configure the network settings of your control device to match the IP address of the VICTOR Nivo instrument. Please note that these instructions are shown for a Windows 10 operating system. 1. Open Control Panel > Network and Internet > Network and Sharing Centre.
  • Page 21 Note that the name of the Ethernet connection can be different. Unplug the Ethernet cable from the reader and re-connect it to see which connection is the right one. A pop up will appear on the screen: 3. Click Properties. VICTOR Nivo – User Manual...
  • Page 22 To avoid IP conflicts, the LAN network to which the reader is connected must have the same first 9 numbers as the reader, but different last three digits. 5. Click OK. VICTOR Nivo – User Manual...

  • Page 23: Installation With Wi-Fi Router

    2. Switch on your control device with wireless network capability (e.g. a laptop). If you are using the optional laptop or tablet from PerkinElmer and start it for the first time, the Windows 10 first start routine will appear. Configure user name, password, language settings etc.

  • Page 24: Making Reader Ip Address A Trusted Site

    If the connection cannot be established, check the router settings. See section 17.1 “Wi-Fi Router Configuration”, page 164. 4. Open your web browser to connect to the VICTOR Nivo. Type http://192.168.0.2 into the address bar and press the Enter key.
  • Page 25 4. If the check box Require server verification (https:) for all sites in this zone is selected, clear the check box. 5. Type http://192.168.0.2 into the field Add this website to the zone and click Add. The reader’s IP address is added to the trusted sites. VICTOR Nivo – User Manual...

  • Page 26: Software Update

    2. Copy the setup file SetupVICTORNivo4-0-X.exe to the PC connected to the VICTOR Nivo. 3. Make sure that the VICTOR Nivo is switched on and connected to the PC (via LAN or WiFi). 4. Close the user interface (web browser).
  • Page 27 3 Reader Installation Notice Please check regularly for software updates on the PerkinElmer website: http://www.perkinelmer.com/lab-products-and-services/resources/software- downloads.html VICTOR Nivo – User Manual...

  • Page 28: Initializing The Reader

    4 Initializing the Reader Initializing the Reader VICTOR Nivo – User Manual...
  • Page 29 Select a user account from the drop down menu, enter the corresponding password in and click OK. See also sections 5.3 “Signing In and Out”, page 37 and 5.4 “User Account Management”, page 39. 4. The reader initialization is started. VICTOR Nivo – User Manual...
  • Page 30 4 Initializing the Reader When the initialization is finished, the PROTOCOLS page will appear on the screen. See chapter 6.2 “User Interface Overview (PROTOCOLS page)”, page 54 for an introduction to the user interface. VICTOR Nivo – User Manual...

  • Page 31: Enhanced Security

    5 Enhanced Security Enhanced Security VICTOR Nivo – User Manual...

  • Page 32: Overview

    FDA under requirements of the Federal Food, Drug, and Cosmetic Act. The Enhanced Security mode of the VICTOR Nivo provides controls and features compatible with 21 CFR Part 11. These features can be classified under three main...

  • Page 33: Getting Started

    Make sure that the time, date and time zone of your PC are set correctly. If you change any of the time settings, you have to switch off the VICTOR Nivo, unplug it from the mains and then restart it to have the time re-synchronized.

  • Page 34: First Login As Security Administrator

    5 Enhanced Security 4. Enter the upgrade code you received from PerkinElmer. 5. Click Enable. The new feature is activated and displayed in the Program info window. See also section 13.4.1 “Manage Features”, page 130. 5.2.4 First Login as Security Administrator 1.

  • Page 35: Defining Security Settings

    See section 5.7 “"Reason for change" Option”, page 51. 5.2.6 Adding Users 1. On the SETTINGS tab, click User account management. You will be prompted again for the SecAdmin password. Enter the password and click Login. 2. Click Create user account. VICTOR Nivo – User Manual...
  • Page 36 Otherwise, if you forget your security admin password, you will no longer be able to access and configure the system. In such a case, access to the system can only be restored by PerkinElmer Service. VICTOR Nivo – User Manual...

  • Page 37: Signing In And Out

    SIGN OUT: Terminates the current session, so that e.g. a different user can sign LOCK: Locks the user interface to prevent unauthorized access. The current session remains active, measurements keep running in the background. Signing out 1. Click the LOCK/SIGN OUT button (upper right corner). VICTOR Nivo – User Manual...
  • Page 38 1. Click the LOCK/SIGN OUT button (upper right corner). 2. Click Lock. The user interface is locked. Unlocking the user interface 1. Enter your password and click Unlock. The session is resumed. 2. To end the session instead, click Close Session. VICTOR Nivo – User Manual...

  • Page 39: User Account Management

    Otherwise, if you forget your security admin password, you will no longer be able to access and configure the system. In such a case, access to the system can only be restored by PerkinElmer Service.

  • Page 40: Management Functions

    (see section 5.4.5 “Security Options”, page 42), his account will be lock automatically. This function is required to unlock such an account. Only currently locked user accounts can be selected. Security options: Opens a window where you can configure password requirements and security settings (see next chapter). VICTOR Nivo – User Manual...

  • Page 41: Passwords And User Names

    User names cannot be used again after deletion of the account. As Full name all characters are allowed, also leaving empty. VICTOR Nivo – User Manual...

  • Page 42: Security Options

    Inactivity limit (minutes): If a user is inactive for a certain period of time (i.e. probably absent), the user interface is locked automatically to prevent unauthorized access. The user can unlock the screen by entering his password. See also section 5.3.2 “Signing Out / Lock”, page 37. VICTOR Nivo – User Manual...

  • Page 43: User Rights (Enhanced Security)

    Start/stop shaking OPTICS Load/unload filter Create filter Edit/delete filter Edit/delete filter Create mirror Edit/delete mirror Edit/delete mirror Load/unload mirror slide Create mirror slide Edit/delete custom mirror slide, edit default mirror slide Delete default mirror slide VICTOR Nivo – User Manual...
  • Page 44 User account management Create new user account Delete user account Group management Change own password Change own full name Set password (of others) Set full name (of others) Unlock account Security options Audit trail Show audit Archive VICTOR Nivo – User Manual...
  • Page 45 5 Enhanced Security Area Task SETTINGS About Manage features Enhanced Security config (reason for change) Backup and restore Backup database Restore database Automatic export settings Other Lock/unlock session VICTOR Nivo – User Manual...

  • Page 46: Electronic Signatures

    2. A new window is opened showing the signature details. Here you can see who signed the result. How to sign protocols 1. Select a protocol on the PROTOCOLS page and click Sign. An electronic signature window is opened. VICTOR Nivo – User Manual...
  • Page 47 An electronic signature window is opened. 2. Select one of the three options (Performed, Reviewed or Approved) to define the meaning of your signature and enter your password. 3. Click Confirm to add the signature. VICTOR Nivo – User Manual...

  • Page 48: Audit Trail

    Successful initialization of the reader IQOQ / Test plate activities Buttons and Elements: Local start date/Local end date: Allows you to select the time period for the audit trail events to be displayed. Timezone: Local time zone (offset from UTC). VICTOR Nivo – User Manual...
  • Page 49 All actions in the selected period of time are displayed. 4. To filter the list by a certain user account: Deactivate the Show all users check box. Select a user from the User drop-down field. VICTOR Nivo – User Manual...
  • Page 50 4. Save the Excel file on your local computer. 5. To free up memory space, click Clean archived data. 6. Click OK to confirm. The archived events are deleted. VICTOR Nivo – User Manual...

  • Page 51: Reason For Change" Option

    Forced: A pop-up window will appear and you cannot continue without entering a reason for change. The Reason for change is displayed as a separate column in the audit trail. See also section 5.6 “Audit trail”, page 48. Example VICTOR Nivo – User Manual...

  • Page 52: Measurement Protocols

    6 Measurement Protocols Measurement Protocols VICTOR Nivo – User Manual...

  • Page 53: Protocols On The Victor Nivo

    Notice When developing your own first protocols with the VICTOR Nivo, start with a copy of a closely matching default protocol. It will help you to better understand the different parameters and workflows used.

  • Page 54: User Interface Overview (Protocols Page)

    To sort the table, click on the corresponding column header. 7. Protocol control buttons Notice You can open the VICTOR Nivo User Manual (PDF) at any time using the Manual button in the bottom right corner of the user interface. VICTOR Nivo – User Manual...

  • Page 55: Protocol Handling

    You can define: Plate format (96 wells, 384 wells …), Plate type (PerkinElmer OptiPlate, PerkinElmer ViewPlate ...), Plate map (which wells are measured), Measurement type (End Point, Scan, Kinetic…), Measurement operations (FI, LUM, ABS, …)
  • Page 56 2. To sort the list of operations, use the Move up and Move down buttons. 3. To edit the measurement settings, click the desired operation in Selected operations and configure the parameters on the right. See also chapter 6.7 “Measurement Settings”, page 70 VICTOR Nivo – User Manual...

  • Page 57: Copying A Protocol

    1. Select the desired protocol and click Copy. A pop-up window will be displayed: 2. Type in the name of the new protocol and click OK. VICTOR Nivo – User Manual...

  • Page 58: Editing A Protocol

    Admin users can delete any custom protocol. Standard users can only delete their own protocols (i.e. logged in with identical user name). 1. On the PROTOCOLS page, select an existing custom protocol and click Delete. A pop-up window for confirming the deletion is opened: VICTOR Nivo – User Manual...

  • Page 59: Exporting A Protocol

    Using the following procedure you can import an existing (previously exported) protocol from a different reader. See also chapter 6.3.5 “Exporting a Protocol”, page 59. 1. On the PROTOCOLS page, click Import. A pop-up window will be opened: VICTOR Nivo – User Manual...

  • Page 60: Signing A Protocol

    Set the mouse cursor into the Protocol Data field. Press Ctrl+V. 5. Click OK 6.3.7 Signing a Protocol The Sign button is only available in Enhanced Security mode for certain user groups. For detailed information please see section 5.5 “Electronic Signatures”, page 46. VICTOR Nivo – User Manual...

  • Page 61: Non-Kinetic Measurements

    Luminescence (LUM) Absorbance with spectrometer (ABS) Absorbance with optical filters (Filter ABS) Alpha laser (ALPHA) Dispenser Temperature Shaker Timing Notice For a detailed description of the measurement parameters see section 6.7 “Measurement Settings”, page 70. VICTOR Nivo – User Manual...
  • Page 62 The difference to fluorescence intensity is that in TRF the lifetime of the emission signal is quite long (µsec, vs. psec in FI). The long lifetime of the emission signal makes it possible to use a delay time between sample excitation and VICTOR Nivo – User Manual...
  • Page 63 Alpha emission signal is generated at shorter wavelengths. The measurement is practically free of unwanted background fluorescence since that is always occurring at longer wavelengths than the excitation VICTOR Nivo – User Manual...
  • Page 64 When the Timing operation starts, the reader waits for a defined period of time and continues the protocol. The user can define, if the delay step is performed inside or outside the reader, indicated by the sub-operations Delay and Delay outside, respectively. VICTOR Nivo – User Manual...

  • Page 65: Kinetic Measurements

    FP Kinetics (Fluorescence polarization) TRF Kinetics (Time-resolved fluorescence) LUM Kinetics (Luminescence) ABS Kinetics (Absorbance with spectrometer) Filter ABS Kinetics (Absorbance with optical filters) Dispensing in Kinetics Shake in Kinetics Delay in Kinetics Delay outside in Kinetics VICTOR Nivo – User Manual...

  • Page 66: Start And End Of Kinetic Sequence

    1. In Available operations, click on Plate Kinetics or Well Kinetics (see description above). In the following example Plate Kinetics will be used. 2. Click on Plate start. The operations Plate Kinetics - Plate start and Plate Kinetics - Plate end are added to the Selected operations. VICTOR Nivo – User Manual...
  • Page 67 In Available operations, select the desired non-kinetic operations. The operations will be added after the kinetic sequence. If necessary, use the Move up/Move down buttons to move an operation to a position above the kinetic sequence. VICTOR Nivo – User Manual...

  • Page 68: Measurement Settings For Kinetic Sequence

    As soon as you select this option, a list of shaking parameters is displayed. For an explanation of these parameters see chapter 6.7.12 “Measurement Settings – Shake”, page 80. Measurement order: Order of wells to be measured. VICTOR Nivo – User Manual...

  • Page 69: Selected Operations List

    One or more operations can be used in the protocol and are part of this list. In the Selected operations list you can change the order of operations as needed. The operations of the protocol are processed from top to bottom. One protocol can include a kinetic sequence and also non-kinetic operations. VICTOR Nivo – User Manual...

  • Page 70: Measurement Settings

    Dual excitation/emission (two measurements with different excitation filters and different emission filters) Excitation filter: Select a filter from the list. Emission filter: Select a filter from the list. Dichroic mirror: Separates the excitation and emission wavelengths, select a mirror from the list. VICTOR Nivo – User Manual...

  • Page 71: Measurement Settings - Fp

    Polarization filters are not needed; they are located inside the reader. Excitation filter: Select a filter from the list. Emission filter S: Select a filter from the list. Emission filter P: Select a filter from the list. VICTOR Nivo – User Manual...
  • Page 72 Instrument settings: High voltage of the photomultiplier tube – gain adjustment. To edit the value, clear the check box. Measurement order: Order of wells to be measured. See picture below for the FP settings (check box Expert mode is selected). VICTOR Nivo – User Manual...

  • Page 73: Measurement Settings - Trf

    Measurement spot size: Select excitation and emission spot sizes from the list. Flash energy: Energy of the individual flash lamp pulse. Measurement order: Order of wells to be measured. See picture below for the TRF settings (check box Expert mode is selected). VICTOR Nivo – User Manual...

  • Page 74: Measurement Settings - Lum

    Only available in Expert mode (can be activated by selecting the check box in the upper right corner): Measurement spot size: Select emission spot size from the list. Measurement order: Order of wells to be measured. See picture below for the LUM settings (check box Expert mode is selected). VICTOR Nivo – User Manual...

  • Page 75: Measurement Settings - Abs

    Only available in Expert mode (can be activated by selecting the check box in the upper right corner): Measurement order: Order of wells to be measured. See picture below for the ABS settings (check box Expert mode is selected). VICTOR Nivo – User Manual...

  • Page 76: Measurement Settings - Filter Abs

    Only available in Expert mode (can be activated by selecting the check box in the upper right corner): Measurement order: Order of wells to be measured. See picture below for the Filter ABS settings (check box Expert mode is selected). VICTOR Nivo – User Manual...

  • Page 77: Measurement Settings - Alpha

    Only after that the next operation in the protocol is started. Reagent 1: Select whether to use dispensing pump 1 or 2 for dispensing the reagent. Dispensing speed: Select one of the five dispensing speeds. Generally speaking, fluids with greater viscosity require slower dispensing speeds. VICTOR Nivo – User Manual...

  • Page 78: Measurement Settings - Dispensing (Kinetic)

    Take care when defining the parameters for dispensing to avoid over filling the wells. This can cause spillage and contamination of the reader. For detailed information on kinetic measurements please see section 6.5 “Kinetic Measurements”, page 65. VICTOR Nivo – User Manual...

  • Page 79: Measurement Settings - Warming On

    Wait until target is reached: If this option is selected, the following operation will start as soon as the target temperature has been reached. If the box is not selected, the next operations will start immediately even if the reader is still warming. VICTOR Nivo – User Manual...

  • Page 80: Measurement Settings - Warming Off

    Double orbital: The plate is shaken in a “number eight” orbit. Shaking speed Slow (300 rpm): Very careful shaking with larger amplitude (suitable for cells). Medium (600 rpm): Medium shaking speed. Fast (1200 rpm): Very efficient mixing. VICTOR Nivo – User Manual...

  • Page 81: Measurement Settings - Delay

    Delay: The plate holder will remain inside the reader during the delay operation. Delay outside: The plate holder will come out during the delay operation. In the Delay parameters you can define the duration of the delay. VICTOR Nivo – User Manual...
  • Page 82 6 Measurement Protocols VICTOR Nivo – User Manual...

  • Page 83: Measuring With The Victor Nivo

    7 Measuring with the VICTOR Nivo Measuring with the VICTOR Nivo VICTOR Nivo – User Manual...

  • Page 84: Plate Load / Unload

    7 Measuring with the VICTOR Nivo Plate Load / Unload 1. Click the OPEN TRAY button in the upper right corner to open the plate loading door. The tray will come out. 2. Place the plate so that its A1 position points to the A1 position indicated in the tray.

  • Page 85: Active / Inactive Protocols

    7 Measuring with the VICTOR Nivo Active / Inactive Protocols Protocols in the protocol list can be either labeled as Active or Not Active. The status of the protocol is shown in parenthesis after the protocol name in the status bar once you have selected it.

  • Page 86: Starting A Measurement

    7 Measuring with the VICTOR Nivo Starting a Measurement 1. Select the protocol you want to measure and make sure that it is Active (indicated by information bar and black protocol name). 2. Optionally: If you first want to edit the protocol, click Edit. The plate map can also be edited directly before starting the protocol (see step 5).

  • Page 87: Showing The Results

    7 Measuring with the VICTOR Nivo 4. Optionally: Enter a comment for the measurement. This comment can be extended after the measurement was finished and is also exported with the measurement results. It is also possible to start the protocol without entering any comments.
  • Page 88 7 Measuring with the VICTOR Nivo The headline above the plate map displays the protocol type and the time that has passed after starting the protocol. From the panel on the left, you can select different options to view the results from the drop-down menus.

  • Page 89: Exporting The Results

    7 Measuring with the VICTOR Nivo Exporting the Results When the measurement is finished, the results can be seen on the screen (Data tab). The data is saved automatically in the instrument. If you have defined an automatic export your results will automatically be saved to the specified location.
  • Page 90 Scan well area measurements are currently not supported. Please also note the VICTOR Nivo export format compatibility information which can be found in the MyAssays Desktop User Guide and in the Release Notes at https://www.myassays.com.

  • Page 91: Signing Out

    7 Measuring with the VICTOR Nivo Signing Out When the measurements are done and results have been exported (if needed) you should sign out from the instrument and allow access for the next user. With Enhanced Security mode enabled please see section 5.3.2 “Signing Out / Lock”, page 37.

  • Page 92: Temperature Control

    8 Temperature Control Temperature Control VICTOR Nivo – User Manual...

  • Page 93: Temperature Control Overview

    Cooling down the reader can take several hours depending on reader temperature and room temperature. The device cannot cool, only heat. Therefore the achievable temperature depends on the room temperature: Minimum: Ambient temperature + 3 °C Maximum: 65 °C. VICTOR Nivo – User Manual...

  • Page 94: Temperature Control Via Main Menu

    The target temperature is indicated by a green bar.  Warming Off: Deactivates temperature control. Notice These general temperature settings can be overridden by warming operations in the protocol. See also chapter 8.3 “Warming Within the Protocol”, page 95. VICTOR Nivo – User Manual...

  • Page 95: Warming Within The Protocol

    Cooling down the reader can take several hours depending on reader temperature and room temperature. Notice Warming operations within a protocol temporarily override the general temperature control settings (configured on the TEMPERATURE page). For further details see chapter 6.7.10 “Measurement Settings – Warming On”, page 79. VICTOR Nivo – User Manual...

  • Page 96: Shaker

    9 Shaker Shaker VICTOR Nivo – User Manual...

  • Page 97: Shaker Overview

    Therefore, PerkinElmer cannot guarantee that corresponding hardware parts reach the expected lifetime. PerkinElmer is not responsible for problems caused by regular excessive shaking. Any effort required to repair the effected hardware components is billable at the current service rate.

  • Page 98: Shaking Within The Protocol

    The shaker can also be used within a protocol by adding a Shake operation to your protocol. You can select the shake type, speed and time. For a detailed description of the measurement settings see chapter 6.7.12 “Measurement Settings – Shake”, page 80. VICTOR Nivo – User Manual...
  • Page 99 9 Shaker Notice In case of a kinetic measurement you could also activate Shake while waiting instead of using a separate shaking operation. See also section 6.5 “Kinetic Measurements”, page 65. VICTOR Nivo – User Manual...

  • Page 100: 10 Handling Optical Components

    10 Handling Optical Components 10 Handling Optical Components VICTOR Nivo – User Manual...
  • Page 101 When loading filters, the system detects whether a filter has been loaded, but not which one. With mirror slides it is not detected at all whether a slide was loaded or unloaded. VICTOR Nivo – User Manual...

  • Page 102: Filters

    Custom filters can be edited and deleted. They are labeled with an open, green lock (   ). A few filter names used in the VICTOR Nivo software do not follow the regular filter nomenclature and are explained in more detail including their function below: 700nm IR Blocker: Generic Luminescence filter as described in the VICTOR Nivo Filter List (700nm short pass (sp), order number: HH35000904).

  • Page 103: Loading A Filter

    (see below). 3. Place the new filter in the loading position. Make sure that the letters on the filter’s label can still be read and are not facing upside down (see figure below). VICTOR Nivo – User Manual...

  • Page 104: Unloading A Filter

    (bright background). 10.1.2 Unloading a Filter 1. Select a present filter (indicated by a bright background) from the list. In the example below, the 260/10 nm filter is selected: VICTOR Nivo – User Manual...
  • Page 105 Always handle the filters with care: Only touch the filter holder, if possible with gloves. Never touch the filter glass to avoid fingerprints. Observe the cleaning instructions (see section 16.3 “Cleaning Optical Components ”, page 160). VICTOR Nivo – User Manual...

  • Page 106: Creating A New Filter

    2. Specify the name and the parameters of the new filter. 3. Click Save and Exit. The new filter is created and added to the filter list. If you click Exit you will return to the OPTICS page without creating a new filter. VICTOR Nivo – User Manual...

  • Page 107: Mirrors

    Otherwise, the software will not be able to match the protocol with the mirror. It will then refuse to start the protocol and indicate that an optical component is missing. VICTOR Nivo – User Manual...

  • Page 108: Mirror Slides

    Changing a Mirror Slide 1. Open the OPTICS page. 2. Click Unload Slide. The lid at the front will open and the mirror slide will be ejected: 3. Remove the mirror slide and replace it with a different slide. VICTOR Nivo – User Manual...

  • Page 109: Editing A Mirror Slide

    The positions are numbered as indicated on the label. Follow the same order as indicated on the label to configure the slide in the software. 3. Click Done to save the changes or Cancel to return to the OPTICS page without saving the changes. VICTOR Nivo – User Manual...
  • Page 110 Do not unscrew mirrors from a slide. If the order of mirrors in a slider need to be changed physically, please reach out to your local PerkinElmer representative to get assistance. VICTOR Nivo – User Manual...

  • Page 111: 11 Dispenser Option

    11 Dispenser Option 11 Dispenser Option VICTOR Nivo – User Manual...
  • Page 112 The tube with the white end connects the left tubing connector of pump 1 to the right hole of the dispenser head. The tube with the yellow end connects the left tubing connector of pump 2 to the left hole of the dispenser head. VICTOR Nivo – User Manual...

  • Page 113: Prime

    Wash/Prime to prime the pump. If necessary, click this button again until the liquid is flowing consistently from the tip. Only the left pump 1 was primed in this example: 4. Repeat the procedure for the right pump 2 (figure below) if needed. VICTOR Nivo – User Manual...
  • Page 114 Press the WASH/PRIME button as many times as needed to ensure that the liquid flows consistently from the tip. The EMPTY button will start emptying the syringe. VICTOR Nivo – User Manual...

  • Page 115: Wash

    For cleaning after the dispense operations, empty the dispenser by priming with air using 2-3 more prime operations, this time with the reservoir container removed: 1. Remove the reservoir container. 2. Open the DISPENSER page. 3. Click Wash/Prime the number of times needed. VICTOR Nivo – User Manual...
  • Page 116 The PUMP SEL button is used to select the pump. Green light indicates the selected pump. The WASH/PRIME button will start the washing of the pump. Priming does not start if the dispensing tip is inside the reader. VICTOR Nivo – User Manual...

  • Page 117: Empty Syringe

    Emptying the syringes can also be performed by using the buttons located at the dispenser unit. The PUMP SEL button is used to select the pump. Green light indicates the selected pump. The EMPTY button will start emptying the syringe. VICTOR Nivo – User Manual...

  • Page 118: 12 Results

    12 Results 12 Results VICTOR Nivo – User Manual...
  • Page 119 If you open the selected result, the function Copy & Edit Protocol allows you to copy and edit the protocol contained in the result. This way, even deleted protocols can be recovered using the results. 1. Select a result and click Open selected result. 2. Click Copy & Edit Protocol. VICTOR Nivo – User Manual...
  • Page 120 12 Results 3. Enter a new name for the protocol and click Copy. The protocol is opened on the PROTOCOLS page and can be edited and saved. VICTOR Nivo – User Manual...

  • Page 121: 13 Settings

    13 Settings 13 Settings VICTOR Nivo – User Manual...
  • Page 122 Manage features: Only available for admin (or SecAdmin in Enhanced Security mode). Activate additional technologies or features for your instrument. See section 13.4 “About”, page 130. Backup and restore: Backup or restore the database. Automatic export settings: Enable/disable automatic export of results. VICTOR Nivo – User Manual...

  • Page 123: Plate Wizard

    The maximum height of 21 mm must not be exceeded by plates or plates including a lid. Plates higher than 21 mm will not enter the measurement chamber and may collide with the outside of the reader. VICTOR Nivo – User Manual...

  • Page 124: Creating A New Plate Type

    1. In Plate formats, select a plate format. 2. In Plates, select the desired plate from the drop-down list. 3. Click Use as default. The selected plate will be displayed as Default plate for selected plate format. VICTOR Nivo – User Manual...

  • Page 125: Run Test Plate Diagnostics

    "Matched with test plate" next to each of the test plate tests to indicate that reference values are available for the inserted plate. If a VICTOR Nivo test plate is available, but the software shows the term “Not matched with test plate”, the test plate diagnostics can still be performed. In this case the test plate results are still checked for reasonable results, but cannot be referenced to initial values.

  • Page 126: Overview Of Tests

    Detects changes in FI bottom position over time For TRF-enabled instruments only Monitors that correct TRF top signal intensity levels are detected ALPHA For Alpha-enabled instruments only Verifies that all Alpha hardware components are functional and calibrated VICTOR Nivo – User Manual...

  • Page 127: How To Run Test Plate Diagnostics

    1. Open the SETTINGS page. 2. Click on Run test plate diagnostics. 3. Enter the serial number of your VICTOR Nivo Test Plate (see label on the plate bottom) and press Enter. 4. Turn the power switch of the VICTOR Nivo Test Plate to “ON”: A.
  • Page 128 If the reader does not pass the tests, verify that the plate was powered on, was inserted in the correct orientation, was clean and undamaged. If the tests still fail after verification, please contact your local PerkinElmer representative. 8. Detailed test results can be viewed and exported on the RESULTS page like normal measurement results.

  • Page 129: Transport Locking

    3. Click Yes and close the browser window. The reader will be locked for transportation. 4. When starting the instrument in the new location, the locks will open automatically. 13.3.3 Unlock When starting the instrument in the new location, it will be unlocked automatically. VICTOR Nivo – User Manual...

  • Page 130: About

    (list of available technologies and features). 13.4.1 Manage Features This function is only available for Admin (or Security Admin) users. It can be used to activate the following features on your VICTOR Nivo (if not available yet): Technology Upgrade Fluorescence Polarization (FP) Time-Resolved Fluorescence (TRF)
  • Page 131 13 Settings The serial number is also printed on the back of the VICTOR Nivo. 3. Send the serial number to your PerkinElmer representative and order the upgrade. In the further process PerkinElmer will send you an upgrade code. How to activate the new feature As soon as you have received the upgrade code from PerkinElmer, you can activate the new feature.

  • Page 132: Backup Or Restore Database

    For more detailed information about user rights see section 13.6 “User Levels (Standard mode)”, page 135. The user rights for the Enhanced Security mode are described in section 5.4.6 “User Rights (Enhanced Security)”, page 43. VICTOR Nivo – User Manual...
  • Page 133 If you want to cancel the backup creation click Stop. 4. As soon as the backup has been completed, it will be listed in the table. 5. To download the backup and save it locally click Download. VICTOR Nivo – User Manual...
  • Page 134 A pop-up window is opened. 3. Click Select a backup file. A file browser is opened. 4. Locate the backup file (*.zip) on your computer and click Open. 5. Click Start backup restore. The backup is uploaded and restored. VICTOR Nivo – User Manual...

  • Page 135: User Levels (Standard Mode)

    View protocol info Start Interrupt protocol run Create new Copy Edit/delete (own) Edit/delete (of other user) Edit/delete Import Export Use adhoc plate map TEMPERATURE Warming on/off Set target temperature SHAKER Set shaking parameter Start/stop shaking VICTOR Nivo – User Manual...
  • Page 136 Delete (of other user) SETTINGS Plate wizard Create new plate / copy plate Use as default (plate / plate format) Edit/delete plate Edit/delete plate Run test plate diagnostics Run all tests in one go Run single tests Transport locking VICTOR Nivo – User Manual...
  • Page 137 13 Settings Area Task SETTINGS About Manage features Backup and restore Backup database Restore database Automatic export settings Enter/exit admin mode (requires password) Change admin password VICTOR Nivo – User Manual...

  • Page 138: Enhanced Security Functions

    5 “Enhanced Security”, page 31. User account management See section 5.4 “User Account Management”, page 39. Audit trail See section 5.6 “Audit trail”, page 48 Enhanced Sec. config. 5.7 “"Reason for change" Option”, page 51 VICTOR Nivo – User Manual...

  • Page 139: Automatic Export Settings

    The format and export type can be selected in the settings. Notice In order to use the automatic export the following conditions for the VICTOR Nivo control device (e.g. laptop) must be met: ®...
  • Page 140 The user is added to the list below. 5. Select the local user and give the user permission level Read/Write.If the local user is the same as the creator of the folder, the permission level is set to Owner. VICTOR Nivo – User Manual...
  • Page 141 How to set up automatic exports 1. Sign in as admin (with Enhanced Security: security admin). 2. On the SETTINGS page, click Automatic export settings. 3. Enable automatic export by activating the check mark at Use automatic export. VICTOR Nivo – User Manual...
  • Page 142 7. To test the connection, click Test. A test file will be written to the shared folder and a message is displayed. 8. Check if the file was written to the shared folder. Click Close. 9. Click Confirm to save the settings. VICTOR Nivo – User Manual...

  • Page 143: 14 Gas Control Option

    14 Gas Control Option 14 Gas Control Option VICTOR Nivo – User Manual...

  • Page 144: Gas Connectors

    14 Gas Control Option An external gas control option is available for VICTOR Nivo readers. This digital CO controller allows to control (and log) the CO and O concentrations by infusing pure and pure N . The CO concentration is adjustable in the range of 0-20 %, the O concentration in the range of 1-20 %.

  • Page 145: Installation

    Keep the plate door of the VICTOR Nivo closed to avoid the escape of gas. Gas supply must be switched off during downtime of the instrument.

  • Page 146: Supplied Equipment

    “N Input” at the gas controller disconnected. 5. 12V DC power supply 6. VICTOR Nivo reader (rear panel) 7. Gas connectors (tubing to be connected using L-shape tubing adapters, item 5 on page 147) 8. N tank + pressure regulator 9.
  • Page 147 Use TUBE AA to connect the “Gas Output” of the gas controller to the lower connector of the VICTOR Nivo reader (use L-shape tubing adapter, see item 5). 3. TUBE AB (x1), 2 m long, blue labeled with PTFE filter Use TUBE AB to connect the “Gas sensors Input”...

  • Page 148: Gas Controller (Rear View)

    Check the tubing for damages each time before using gas control. Do not operate the instrument with a damaged gas supply tubing. Contact PerkinElmer to request a new tubing. Do not manipulate the gas tubing or fittings. The tubing may only be shortened using an approved hose cutter.

  • Page 149: Installing Tubes Between Gas Tanks And Gas Controller

    To remove tubing from push-in connectors, push the red rubber ring while pulling the tubing. If the tubing does not easily come out, do not force it and simply make sure the red ring is properly pushed. VICTOR Nivo – User Manual...
  • Page 150 Push the tube into the L-shape adapter. 3. Use TUBE AB to connect the Gas sensors Input of the gas controller to the upper gas connector of the reader. Push the tube into the L-shape adapter. VICTOR Nivo – User Manual...

  • Page 151: Setting The Pressure

    0.5 bar: A. Pull the knob up to release the lock. B. Rotate the knob until the pressure reaches the desired value (clockwise/counter clockwise to increase/decrease the pressure). C. Push the knob down to lock it. VICTOR Nivo – User Manual...
  • Page 152 2. Turn on the gas controller using the switch on the lower right of the front panel. 14.4.2 Check Basic Settings of Gas Controller The gas controller is delivered with factory preset settings for use with VICTOR Nivo. To use the optimal parameters for your working conditions, please perform the following steps: 1.
  • Page 153 (0.5 bar), you could see an overshoot/undershoot on the CO and O compositions. In this case, we recommend changing the Time value slightly in a range of [2–5] for O and [3–10] for CO VICTOR Nivo – User Manual...
  • Page 154 In this case you don’t regulate O levels. The touch screen allows you to switch from standard CO mode to CO mode and back: VICTOR Nivo – User Manual...
  • Page 155 14 Gas Control Option 1. Go to the homepage. 2. Tap on the O2 tab. 3. Tap + until ‘Air’ appears. 4. Tap Set to save. VICTOR Nivo – User Manual...

  • Page 156: Remote Control

    15 Remote Control 15 Remote Control VICTOR Nivo – User Manual...
  • Page 157 15 Remote Control The VICTOR Nivo reader can also be used for measurements in an automated environment. In this case the reader is controlled by external scheduling software. As long as the reader is under remote control, no user can log into the web interface.

  • Page 158: Viewing Results

    1. Type http://192.168.0.2/results into the address bar of your browser and press the Enter key. 2. Click All (zip) to download a ZIP file containing all result files. 3. Afterwards, it is recommended to delete all result files to free up internal memory: Click Delete All. VICTOR Nivo – User Manual...

  • Page 159: Maintenance

    16 Maintenance 16 Maintenance VICTOR Nivo – User Manual...

  • Page 160: Removing Dust

    5. Take care that no flow marks are visible, if that is the case, repeat the procedure. Notice It is recommended that the optical filters are always kept in the instrument’s own filter storage. VICTOR Nivo – User Manual...

  • Page 161: Changing The Air Filter

    Specification of filter material: 92 mm air filter made of polyurethane foam 45 pores per inch (ppi) PerkinElmer spare part order number: "HH35900006 VICTOR Nivo Air Inlet Filter" Warning! Switch off the instrument and unplug from mains before changing the air filter.

  • Page 162: Dispenser Maintenance

    On a weekly or monthly basis, flush with 0.1-0.5 N sodium hydroxide (NaOH), followed by neutralization with an equivalent normality (0.1-0.5 N) of hydrochloric acid (HCl). Rinse with pure water to remove the HCl. VICTOR Nivo – User Manual...

  • Page 163: Troubleshooting

    17 Troubleshooting 17 Troubleshooting VICTOR Nivo – User Manual...
  • Page 164 SSID and click Connect. 3. Once prompted enter the security key. This is found on the rear of the router (Wireless Password). Select work network and close. VICTOR Nivo – User Manual...
  • Page 165 Press Enter. 5. Enter the user name and the password (printed on the back of the router, see above). Click Login. 6. Click Next to enter quick setup. 7. Select Access Point and click Next. VICTOR Nivo – User Manual...
  • Page 166 8. The following screen appears (your SSID and wireless password will be different from this example). Click Next. 9. Select LAN type Static IP. Check that the IP address matches the one shown below and that DHCP Server is enabled. Click Next. VICTOR Nivo – User Manual...
  • Page 167 17 Troubleshooting 10. Scroll down the next page and click Finish. Click Reboot. 11. The router will now re-boot. Wait until the reboot is complete and try connecting again. VICTOR Nivo – User Manual...
  • Page 168 VICTOR Nivo logs its processes and these log files can also be accessed by a user. To collect these log files, do the following: 1. Enter the IP address of the VICTOR Nivo in the address bar of the browser as usual.

  • Page 169: Specifications

    18 Specifications 18 Specifications VICTOR Nivo – User Manual...

  • Page 170: Reader Specifications

    Z-focus range 0 – 16 mm Shaking modes Linear, orbital, double orbital Number of optical filters Up to 32 Number of dichroic mirrors Up to 5 per slide Instrument connection Ethernet Dispenser unit (optional) 2 injectors VICTOR Nivo – User Manual...

  • Page 171: Instrument Models

    < 3 mP (96- and 384-well plate) TRF detection limit (Europium) < 2.3 amol/well (96-well plate) < 0.5 amol/well (384-well plate) TRF dynamic range 5 orders of magnitude LUM detection limit (ATP) < 50 amol (96-well plate) VICTOR Nivo – User Manual...

  • Page 172: Environmental Conditions

    50 % at 40 °C Transportation and storage conditions: -20 °C to +50 °C, relative humidity 5 to 90 %, IEC 68-2-56 guidelines provided in transportation package 18.1.8 Electrical Requirements One individual power socket is required for each system component: VICTOR Nivo – User Manual...
  • Page 173 50/60 Hz 2.0 m 18.1.9 Property Value Size of internal data storage 12 GB Supported web browser Recommended and tested: (of control device) Google Chrome Microsoft Edge (Legacy/Chromium) Microsoft Internet Explorer is no longer supported. VICTOR Nivo – User Manual...
  • Page 174 18 Specifications 18.2 VICTOR Nivo Test Plate (Optional) Battery-operated test plate Order number: HH35000795 A. ON / OFF switch B. Status LED Battery status (green, orange, red) C. Battery (1x AAA) D. Photodiode (communication between plate and instrument) Pre-defined protocol in the control software tests specifications and performance of...
  • Page 175 19 IT Policy 19 IT Policy VICTOR Nivo – User Manual...

  • Page 176: Web Browser

    These settings are also applied to the VICTOR Nivo and the control software. If you change any of the time settings on the control device, you have to switch off the VICTOR Nivo, unplug it from the mains and then restart it to have the time re-synchronized. VICTOR Nivo – User Manual...
  • Page 177 20 Index 20 Index VICTOR Nivo – User Manual...
  • Page 178 Cleaning spillage 160 Dispenser maintenance 162 Close tray 84 Dispenser option 111 CO2 control 143 Dispensing (kinetic) Collecting log files 168 Measurement settings 78 Computer configuration 20 Dispensing (non-kinetic) Computer hardware 17 Measurement settings 77 VICTOR Nivo – User Manual...
  • Page 179 Fluorescence Intensity (FI) 62 Kinetic measurements 65 Fluorescence Polarization (FP) 62 Kinetic sequence FP 62 Define 66 Measurement settings 71 Measurement settings 68 Free up internal memory 168 Start and end 66 Gas concentrations 154 LAN cable 20 VICTOR Nivo – User Manual...
  • Page 180 Open tray 84 ALPHA 77 Operation Delay 81 ALPHA 63 Dispensing (kinetic) 78 Dispenser 64 Dispensing (non-kinetic) 77 FI 62 FI 70 Filter ABS 63 Filter ABS 76 FP 62 FP 71 LUM 63 LUM 74 VICTOR Nivo – User Manual...
  • Page 181 Saving a protocol 57 Copying a protocol 57 Selected operations 56, 69 Create 55 Serial number 121 Delete 58 SETTINGS page 121 Edit 58 Shake Export 59 Measurement settings 80 Import 59 Shake (operation) 64 Save 57 VICTOR Nivo – User Manual...
  • Page 182 User rights (Enhanced Security) 43 Software update 26 Specifications 169 VICTOR Nivo Test Plate 125 Spillage cleaning 160 VICTOR Nivo Test Plate (specs) 174 Standard user 135 Viewing results (remote control) 158 Starting a measurement 86 Starting up the reader 28...
  • Page 184 PerkinElmer Schnackenburgallee 114 22525 Hamburg Germany Internet: http://www.perkinelmer.com email: info@perkinelmer.com...

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