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Recommendation:
a separation, you can use weaker solvents such as ethanol or isopropanol.
•
Set the system parameters:
•
Flow rate using 2.5 mL/min for a 3.0 × 100-mm ID, sub-2 μm column.
•
Flow rate using 2.5 mL/min for a 3.0 × 100-mm ID, 2.5-μm Waters chiral column.
Exception:
•
Column temperature above the minimum 31° C.
•
System pressure above 9997 kPa (100 bar, 1450 psi).
Tip:
•
ABPR pressure set to 13,789 kPa (138 bar, 2000 psi)
After you review the data and choose the column best suited for your application, you can run an
isocratic method or a shallow gradient to further optimize your method.
2.6
Shutting down the ACQUITY UPC
To shut down the ACQUITY UPC
1.
Pump the initial mobile phase mixture through the column.
Result:
Doing so maintains the column equilibrium necessary for good retention time
reproducibility.
2.
To lengthen lamp life, extinguish the detector lamp by clicking Lamp Off in the detector
control panel.
Instruction:
your shutdown method is deactivated.
3.
If the next injection is not for several hours, slow the flow rate in the interim to approximately
0.3 mL/min to conserve solvent.
Instruction:
4.
Ensure that the detector is operating and that the column compartment is at operating
temperature during this period.
To shut down the ACQUITY UPC
Warning:
unplug the instrument's power cord from the AC outlet, which completely interrupts power.
The power switch on each system instrument controls the basic operational state of that
instrument. Nevertheless, some instrument circuits remain live after the instrument is
switched off.
1.
Extinguish the detector lamp by clicking Lamp Off in the detector control panel.
2.
Remove buffer salts and additives by flushing the system with water.
3.
Flush the column and flow cell with 100% pure organic solvent.
Requirement:
shutdown overnight to prevent column failure.
Start with methanol as your co-solvent. If the column does not achieve
For smaller-ID columns and smaller particle sizes, use lower flow rates.
A good system pressure to being with is 11,996 kPa (120 bar, 1740 psi).
2
system for less than 24 hours:
If you are running under MassLynx control, ensure that Auto-Shutdown for
Ensure that the shutdown method is deactivated.
2
system for more than 24 hours:
To avoid electric shock, set a system instrument's power switch to Off, and then
The system must remain in 100% CO
November 16, 2016, 715004521 Rev. C
2
system
at the end of operation and during
2
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