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Waters ACQUITY UPC2 System Manual

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ACQUITY UPC
System Guide
Copyright © Waters Corporation
715004521
2016
All rights reserved
Revision C

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Summary of Contents for Waters ACQUITY UPC2

  • Page 1 ACQUITY UPC System Guide Copyright © Waters Corporation 715004521 2016 All rights reserved Revision C...
  • Page 2 November 16, 2016, 715004521 Rev. C Page ii...

  • Page 3: Trademarks

    This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).

  • Page 4: Contacting Waters

    Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide.

  • Page 5: Fcc Radiation Emissions Notice

    Hand crush hazard Warning: To avoid crushing your fingers beneath or between modules, use extreme care when installing a module in the system stack. High voltage hazard Warning: To avoid electric shock, do not remove protective panels. The components they cover are not user-serviceable.

  • Page 6: Electrical Power Safety Notice

    interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Electrical power safety notice Do not position the instrument so that it is difficult to disconnect the power cord. Equipment misuse notice If equipment is used in a manner not specified by its manufacturer, protections against personal injury inherent in the equipment’s design can be rendered ineffective.

  • Page 7: Audience And Purpose

    Electrical and electronic equipment with this symbol may contain hazardous substances and should not be disposed of as general waste. For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. Serial number Part number catalog number...

  • Page 8: Ism Classification: Ism Group 1 Class B

    Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. EC authorized representative Address Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone:...

  • Page 9: Table Of Contents

    ............13 1.1.1 Comparing supercritical fluid properties to those of other solvents ....... 14 1.2 System configurations ....................... 14 1.2.1 ACQUITY UPC2 core system stack configurations and supported modules ....15 1.2.2 Major components of the ACQUITY UPC system............16 1.2.3 Waste collection containers ...................
  • Page 10 2.2 Starting the console software ....................23 2.2.1 Starting the console ....................... 24 2.3 Priming and equilibrating the system ..................25 2.3.1 Priming the system ......................25 2.3.2 Equilibrating the system....................26 2.4 Observing the sample-injection sequence ................26 2.5 Performing column screening ....................32 2.6 Shutting down the ACQUITY UPC system ................
  • Page 11 A.3 Bottles Prohibited symbol ......................85 A.4 Required protection ........................85 A.5 Warnings that apply to all Waters instruments and devices ............86 A.6 Warnings that address the replacing of fuses ................89 A.7 Electrical and handling symbols ....................90 A.7.1 Electrical symbols......................
  • Page 12 B Specifications ....................... 93 B.1 System specifications ....................... 93 B.2 Solvent specifications ....................... 95 B.3 Wetted materials ........................95 B.4 ccBSM specifications ........................ 96 B.5 ccManager specifications ......................98 B.6 ACQUITY column manager with active preheating and auxiliary column manager specifications ......................99 B.7 ACQUITY 30-cm single-zone column manager specifications ..........

  • Page 13: Acquity Upc System

    (CO ) as the primary mobile phase for normal-phase, chiral, and achiral separations. You control the system using the driver pack software that you access through one of these Waters’ data systems: Empower software or MassLynx software. ®...

  • Page 14: Comparing Supercritical Fluid Properties To Those Of Other Solvents

    1.1.1 Comparing supercritical fluid properties to those of other solvents Supercritical CO fluid has a lower viscosity, higher diffusion rate, and no surface tension when compared to liquid CO . Supercritical CO physical properties are between those of liquids and gases.

  • Page 15: Acquity Upc2 Core System Stack Configurations And Supported Modules

    1.2.1 ACQUITY UPC core system stack configurations and supported modules Figure 1–2: Core 1 configuration (2-column system) Side mount solvent tray CM-A ccManager SM-FL ccBSM 73 Figure 1–3: Core 2 configuration (4-column system) Solvent tray ccManager SM-FL CM-AUX CM-A ccBSM TP03523 November 16, 2016, 715004521 Rev.

  • Page 16: Major Components Of The Acquity Upc

    Figure 1–4: Core 3 configuration (6-column system) Solvent tray ccManager CM-AUX SM-FL CM-A ccBSM CM-AUX TP03529 Figure 1–5: Core 4 configuration (up to 8-column system) Solvent tray ccManager CM-30S SM-FL ccBSM See also: Appendix C for information about configuring a system with two CM-30S modules. 1.2.2 Major components of the ACQUITY UPC system...
  • Page 17 Table 1–2: Major system components Module Description Regulates and monitors the incoming flow and pressure of liquid CO ACQUITY UPC in the system through these mechanisms: Convergence manager (ccManager) • Electronically controlled CO inlet shutoff valve • Overpressure protection • Auxiliary valve for venting the injection loop •...

  • Page 18: Waste Collection Containers

    1.2.3 Waste collection containers Use only waste collection containers required by your local regulating agency with the ACQUITY system. Warning: To avoid serious injury never use a glass waste container with an ACQUITY system because it can burst in the event of over-pressure conditions. Notice: To avoid fluid backup, ensure proper drainage of waste: •...
  • Page 19 Figure 1–6: Solvent flow path through a Core 1 system November 16, 2016, 715004521 Rev. C Page 19...
  • Page 20 November 16, 2016, 715004521 Rev. C Page 20...

  • Page 21: Preparing For Operation

    Preparing for operation For normal-phase separations, you can specify pressures of 10,342 to 41, 369 kPA, (103 to 413 bar, 1500 to 6000 psi) and temperatures of 4º to 90º C. Perform the tasks in this section to prepare the system for use. Prerequisite: Make sure you have performed all procedures in the module overview and maintenance guides in the preparing for operation sections before proceeding.

  • Page 22: Powering-On The System Modules

    Where required, tighten system fittings according to the procedures set forth in the overview and maintenance guides for each instrument. Note particularly that the techniques used for retightening fittings differ from those used to install fittings for the first time. 2.1.2 Powering-on the system modules Requirement:...

  • Page 23: Starting The Console Software

    You can start the console from within the data system that you are using. See also: The Empower software or MassLynx software online Help for details about starting and logging on to the data system. Refer also to the Waters Console online Help. November 16, 2016, 715004521 Rev. C Page 23...

  • Page 24: Starting The Console

    To start the console within a data system: On the workstation computer, start the chromatography data system, and log on. Start the console from within the data system that you are using: • In Empower software, in the Run Samples window, click Display console right-click, and select a menu item.

  • Page 25: Priming And Equilibrating The System

    Figure 2–2: Console system view Flow and lamp status Control panels show current Navigate the tree to view and configure instrument conditions indicators instrument settings, view interactive displays and plots, set maintenance counters, and view logs Priming and equilibrating the system The CO side of the pump primes itself when you start the system and the flow is not turned on.

  • Page 26: Equilibrating The System

    2.3.2 Equilibrating the system Equilibrating the system involves setting the conditions for the method you plan to run for your analysis. For example, setting the flow rate, solvent composition, column temperature, ABPR pressure and other such conditions. See: The ACQUITY UPC Binary Solvent Manager Operator’s Overview and Maintenance Guide for details about priming and equilibrating the system.
  • Page 27 Figure 2–4: Step 2: SM-FL aspirates the sample (PLNO) Auxiliary valve (load position) Waste Waste Sample To column From pump Sample loop Injection valve (inject position) Metering syringe Sample Liquid mobile phase (CO Buffer volume Gas mobile phase (CO Sample Air gap Weak wash The SM-FL moves the needle to the programmed vial position and aspirates an air gap.
  • Page 28 Figure 2–5: Step 3: Metering syringe repositions the sample Auxiliary valve (load position) Waste Waste To column From pump Sample Sample loop Injection valve (inject position) Metering syringe Sample Liquid mobile phase (CO Buffer volume Gas mobile phase (CO Sample Air gap Weak wash When the needle is over the injection port, the metering syringe pulls the sample aliquot past port...
  • Page 29 Figure 2–6: Step 4: Metering syringe positions the sample in the sample loop Auxiliary valve (load position) Waste Waste To column From pump Sample loop Sample Injection valve (Load position) Metering syringe Sample Liquid mobile phase (CO Buffer volume Gas mobile phase (CO Sample Air gap Weak wash...
  • Page 30 Figure 2–7: Step 5: Injection valve moves to the inject position Auxiliary valve (load position) Waste Waste To column From pump Sample Sample loop Injection valve (inject position) Metering syringe Sample Liquid mobile phase (CO Buffer volume Gas mobile phase (CO Sample Air gap Weak wash...
  • Page 31 Figure 2–8: Step 6: Auxiliary valve introduces sample into the liquid CO system Auxiliary valve (inject position) Waste Waste Sample To column From pump Sample loop Injection valve (inject position) Metering syringe Sample Liquid mobile phase (CO Buffer volume Gas mobile phase (CO Sample Air gap Weak wash...

  • Page 32: Performing Column Screening

    Figure 2–9: Step 7: The SM-FL washes the outside and inside of the needle Auxiliary valve (inject position) Waste Waste To column From pump Wash syringes Sample loop Injection valve (inject position) Metering syringe Sample Liquid mobile phase (CO Buffer volume Gas mobile phase (CO Sample Air gap...

  • Page 33: Shutting Down The Acquity Upc System

    Flow rate using 2.5 mL/min for a 3.0 × 100-mm ID, sub-2 μm column. • Flow rate using 2.5 mL/min for a 3.0 × 100-mm ID, 2.5-μm Waters chiral column. Exception: For smaller-ID columns and smaller particle sizes, use lower flow rates.
  • Page 34 Depending on whether the system includes a CM-A or a CM-30S, do as follows: • CM-A systems - Select “Shut down all columns”, to override all temperature settings. • CM-30S systems - Select Off as the temperature setting. Power-off the detector. Stop the solvent flow to vent the system: •...

  • Page 35: Method Parameters

    Method parameters Creating methods for UPC normal-phase chromatography is similar to creating them for HPLC normal-phase chromatography, the sole difference being the replacement of water with liquid CO Creating methods in Empower software In Empower software, you first create an instrument method in a method set to specify your instrument parameters.

  • Page 36: Sm-Fl Instrument Method Settings

    3.2.1.1 Adding co-solvent to adjust the retention times of sample components Adding a co-solvent typically decreases a sample’s retention time. System pressure and temperature also effect retention times. Adding 0 to 20% co-solvent to CO As co-solvent Retention time Solvating increases decreases power...
  • Page 37 3.2.4.1 Setting up a pressure gradient Some classes of compounds require the use of 100% liquid CO to elute off the column. When using 100% liquid CO , you can set up a pressure gradient to increase the solvent strength (density) of the liquid CO , which is similar to setting up a solvent gradient to increase solvent strength.

  • Page 38: Acquity Upc Pda Instrument Method Settings

    • When specifying column managers, note that they are configured as detectors. • Waters pump control must be configured as the pump when the ccBSM, ISM or 515 makeup pump is used. • You create methods offline. During offline method creation, all possible column positions, temperature-set zones, and external valve positions are available.

  • Page 39: External Connections

    • Contact Waters’ Technical Service department before moving the ACQUITY UPC system modules. • If you must transport a system component, or remove it from service, contact Waters’ Technical Service department for recommended cleaning, flushing, and packaging procedures. Warning: To avoid back injuries, do not attempt to lift the system modules without assistance.

  • Page 40: External Connections

    SM-FL. The SM-FL is connected internally to the Ethernet switch. Requirement: If you are running more than six modules in addition to the SM-FL, use a Waters Ethernet switch box. November 16, 2016, 715004521 Rev. C...

  • Page 41: Connecting External Signal Cables

    Warning: To avoid electric shock, always disconnect the system from its source of electrical power before connecting or disconnecting external cables. 4.2.0.1 Required materials • 9/32-inch nut driver • Flat-blade screwdriver • Connector • Signal cable 4.2.1 Connecting external signal cables Refer to the signal connection location shown on the silk-screened label affixed to the rear panel of each system module.
  • Page 42 Tip: Use the 9/32-inch nut driver to tighten the locking nut until the fork terminal does not move. Fork terminal Locking nut Grounding stud 4.2.1.1 Connecting the ccBSM I/O signal cables The rear panel of the ccBSM includes two removable connectors that hold the screw terminals for I/O signal cables.
  • Page 43 For electrical specifications, see the ACQUITY UPC System Specifications Guide. Table 4–1: ccBSM analog-out/event-in connections Signal connection Description Auxiliary 1 In Reserved for future use. Auxiliary 2 In Reserved for future use. Run Stopped Out Indicates (with a contact closure) the ccBSM stopped operating because of an error condition or operator request.

  • Page 44: Connecting The Upc Pda Detector Signal Cables

    Use only the power-supply cable provided when the system module was installed. • Use SVT-type power cord in the United States and HAR-type power cord, or better, in Europe. For requirements elsewhere, contact your local Waters distributer. • Inspect the power cord for damage, and replace it, if necessary.
  • Page 45 Figure 4–4: FlexCart power connections To circuit A AC line Network switch (if needed) SM-FL ccBSM FlexCart power strips ccManager LCD/monitor Computer workstation CM-A, CM-AUX or CM-30S To circuit B AC line November 16, 2016, 715004521 Rev. C Page 45...
  • Page 46 November 16, 2016, 715004521 Rev. C Page 46...

  • Page 47: Splitter Configurations

    Splitter configurations Splitter components The following section defines the UPC splitter components by supported core configuration and detection method. Notes: • To comply with Japan’s High Pressure Gas Safety Act, Japanese customers must use stainless steel tubing instead of PEEK™ tubing. •...
  • Page 48 Figure 5–2: MS splitter (PEEK) 26 inch x .007 inch ID From the column manager or PDA 50 inch x .010 inch ID 59 inch x .005 inch ID From the makeup pump union or To the ccManager ISM tee Bracket (Note: The union is connected to the 8-inch makeup pump...

  • Page 49: Components Of The Core 4 Splitter

    Requirement: The ABPR “health” check cannot be performed when a splitter is connected in the solvent path. Temporarily isolate the flow cell's PDA-detector outlet from the inlet tee of the ccManager. 5.1.2 Components of the core 4 splitter Figure 5–4: ELSD splitter Bracket 29.5 inch x...

  • Page 50: Isocratic Solvent Manager Splitter

    Figure 5–6: MS splitter (SST) 46 inch x .007 inch ID From the CM-30S or PDA 67 inch x .004 inch ID 50 inch x .010 inch ID From the check valve outlet port To the ccManager on the 515 makeup pump or, from the ISM tee Bracket µ...
  • Page 51 Figure 5–7: ISM splitter module for dual detection 0.010 inch ID PEEK 0.007 inch ID From the PDA PEEK To the ccManager detector Figure 5–8: ISM splitter module for triple detection 0.007 inch ID From the PDA 75 µm ID To the PEEK PEEKsil™...

  • Page 52: Splitter Bracket Mount Locations

    Splitter bracket mount locations Figure 5–9: Splitter bracket mount location on the SM-FL Bottle tray ccManager bracket Splitter bracket SM-FL TP03510 Figure 5–10: Splitter bracket mount location on the CM-A Splitter bracket Column manager ccManager Bottle tray bracket TP03511 November 16, 2016, 715004521 Rev. C Page 52...
  • Page 53 Figure 5–11: Splitter bracket mount location on the CM-AUX Splitter bracket CM-Aux TP03508 Figure 5–12: Splitter bracket mount location on the CM-30S Splitter bracket CM-30S November 16, 2016, 715004521 Rev. C Page 53...

  • Page 54: Core 1 Configuration: System Diagrams And Tubing Connections

    Figure 5–13: Splitter bracket mount location on the PDA Splitter bracket TP03507 Figure 5–14: Splitter bracket mount location on the 515 makeup pump 515 makeup pump Splitter bracket Core 1 configuration: system diagrams and tubing connections Note: The figures in this section describe recommended splitter bracket mounting locations. If you choose other locations to mount the splitter assembly bracket, ensure that the splitter tubing connections are not pinched or constrained, and that you follow safe laboratory practices for leak containment.
  • Page 55 Figure 5–15: Core 1 configuration with a PDA, ELSD, and splitter ELSD ccManager Solvent tray ELSD splitter mounted to the SM-FL TP03523 ccBSM SM-FL CM-A The following table describes final tubing connections and does not list factory-installed tubing connections. Table 5–1: Core 1 configuration with a PDA, ELSD, and splitter Notes: Tube...
  • Page 56 Figure 5–16: Core 1 configuration with a PDA, SQD, and splitter CM-A MS splitter mounted to CM-A Solvent tray Pump control module ccBSM TP03524 SM-FL 515 makeup pump ccManager The following table describes final tubing connections and does not list factory-installed tubing connections.
  • Page 57 Table 5–2: Core 1 configuration with a PDA, SQD, and splitter Notes: Tube Tube description From recommended trim length 29.5 inch PEEKsil Splitter module SQD probe 50 µm Do not trim tubing (pre-connected to the splitter) 50 inch Splitter module Left side of the 0.010 inches Do not trim more PEEK tubing...
  • Page 58 Figure 5–17: Core 1 configuration with a PDA, TQ-XS, and splitter CM-A MS splitter mounted to CM-A Solvent tray TQ-XS Pump control module ccBSM SM-FL 515 makeup pump ccManager The following table describes final tubing connections and does not list factory-installed tubing connections.
  • Page 59 Table 5–3: Core 1 configuration with a PDA, TQ-XS, and splitter Notes: Tube Tube description From recommended trim length 750 mm (29.5 inch) TQ-XS inlet Splitter module 50 µm Do not trim tool-free ESI (or probe APCI) probe capillary (connects to external, V-detail fitting on splitter) (Note: To connect...
  • Page 60 Figure 5–18: Core 1 configuration with a PDA, ISM, and QDa ccManager CM-A Solvent tray SM-FL ISM with splitter ccBSM splitter module 73 The following table describes final tubing connections and does not list factory-installed tubing connections. Table 5–4: Core 1 configuration with a PDA, ISM, and QDa Notes: Tube Tube...
  • Page 61 Figure 5–19: Core 1 configuration with a PDA, ELSD, ISM, and QDa Solvent tray ELSD CM-A ccManager SM-FL ISM with splitter splitter module ccBSM 73 The following table describes final tubing connections and does not list factory-installed tubing connections. Table 5–5: Core 1 configuration with a PDA, ELSD, ISM, and QDa Notes: Tube...

  • Page 62: Core 2 Configuration: System Diagrams And Tubing Connections

    Core 2 configuration: system diagrams and tubing connections Figure 5–20: Core 2 configuration with a PDA, ELSD, and splitter ELSD splitter module mounted on the PDA Solvent tray ELSD ccManager SM-FL CM-AUX ccBSM CM-A TP03524 The following table describes final tubing connections and does not list factory-installed tubing connections.
  • Page 63 Table 5–6: Core 2 configuration with a PDA, ELSD, and splitter Notes: Tube Tube description From recommended trim length 29.5 inch PEEKsil Splitter module ELSD 50 µm Do not trim tubing (pre-connected to the splitter) 46 inch CM-A or PDA Splitter module 0.007 inches Do not trim more PEEK tubing than 30 inches...
  • Page 64 Table 5–7: Core 2 configuration with a PDA, SQD, and splitter Notes: Tube Tube description From recommended trim length 29.5 inch PEEKsil Splitter module Mass spectro- 50 µm Do not trim tubing meter (pre-connected to the splitter) 50 inch Splitter module Left side of the 0.010 inches Do not trim more PEEK tubing...

  • Page 65: Core 3 Configuration: System Diagrams And Tubing Connections

    Core 3 configuration: system diagrams and tubing connections Figure 5–22: Core 3 configuration with a PDA, ELSD, and splitter ccManager Solvent tray ELSD ELSD splitte module mounted on the PDA SM-FL CM-AUX CM-A CM-AUX ccBSM TP03530 The following table describes final tubing connections and does not list factory-installed tubing connections.
  • Page 66 Table 5–8: Core 3 configuration with a PDA, ELSD, and splitter Notes: Tube Tube description From recommended trim length 29.5 inch PEEKsil Splitter module ELSD 50 µm Do not trim tubing (pre-connected to the splitter) 46 inch CM-A or PDA Splitter module 0.007 inches Do not trim more PEEK tubing than 30 inches...
  • Page 67 Table 5–9: Core 3 configuration with a PDA, SQD, and splitter Notes: Tube Tube description From recommended trim length 29.5 Splitter module Mass 50 µm Do not trim PEEKsil tubing spectrometer (pre-connected to the splitter) 50 inch Splitter module Left side of the 0.010 inches Do not trim more PEEK tubing ccManager tee...
  • Page 68 Figure 5–24: Core 3 configuration with a PDA, ISM, and QDa Solvent tray CM-AUX CM-A ccManager CM-AUX SM-FL ISM with UPC2 splitter module ccBSM ISM splitter module 73 The following table describes final tubing connections and does not list factory-installed tubing connections.
  • Page 69 Figure 5–25: Core 3 configuration with a PDA, ELSD, ISM, and QDa ccManager ELSD Solvent tray SM-FL CM-A ISM splitter module 73 ISM with UPC CM-AUX ccBSM splitter The following table describes final tubing connections and does not list factory-installed tubing connections.

  • Page 70: Core 4 Configuration: System Diagrams And Tubing Connections

    Core 4 configuration: system diagrams and tubing connections Figure 5–26: CM-30S column configuration November 16, 2016, 715004521 Rev. C Page 70...
  • Page 71 Figure 5–27: Core 4 configuration with a PDA, ELSD, and splitter ccManager Solvent tray ELSD splitter module mounted on the CM-30S 73 SM-FL ccBSM ELSD CM-30S The following table describes final tubing connections and does not list factory-installed tubing connections. November 16, 2016, 715004521 Rev.
  • Page 72 Table 5–12: Core 4 configuration with a PDA, ELSD, and splitter Notes: Tube Tube description From recommended trim length 29.5 inch Splitter module ELSD 50 µm Do not trim PEEKsil (pre-connected to the splitter) 46 inch CM-30S or PDA Splitter module 0.007 inches Do not trim (pre-connected to more than...
  • Page 73 Table 5–13: Core 4 configuration with a PDA, SQD, and splitter Notes: Tube Tube description From recommended trim length 29.5 inch Splitter module Mass spectro- 50 µm Do not trim PEEKsil tubing meter (pre-connected to the splitter) 50 inch Splitter module Left side of the 0.010 inches Do not trim PEEK tubing...
  • Page 74 Figure 5–29: Core 4 configuration with a PDA, ISM, and QDa Solvent tray ccManager ISM with splitter SM-FL ccBSM CM-30S The following table describes final tubing connections and does not list factory-installed tubing connections. Table 5–14: Core 4 configuration with a PDA, ISM, and QDa Notes: Tube Tube...
  • Page 75 Figure 5–30: Core 4 configuration with a PDA, ELSD, ISM, and QDa Solvent tray ccManager CM-30S ELSD ISM with UPC2 splitter splitter module SM-FL ccBSM The following table describes final tubing connections and does not list factory-installed tubing connections. Table 5–15: Core 4 configuration with a PDA, ELSD, ISM, and QDa Notes: Tube...
  • Page 76 November 16, 2016, 715004521 Rev. C Page 76...

  • Page 77: Maintenance

    Maintenance This chapter provides the maintenance guidelines and procedures necessary to maintain the instrument’s performance. Follow a maintenance schedule, and perform maintenance as required and described in this chapter. Maintaining the ACQUITY UPC system modules Consult the following documentation on the ACQUITY UPC documentation CD for details on maintaining the ACQUITY UPC system modules:...
  • Page 78 Note: If your system has a CM-30S, you must complete these tasks bfore before testing the ABPR: • Set the operation mode to enable Bypass. • Temporarily, install a union in place of the column in position 8 of the CM-30S. If your system includes two CM-30S column managers, repeat these tasks for the second column manager.

  • Page 79: Spare Parts

    Connections INSIGHT is an “intelligent” device management (IDM) Web service that enables Waters to provide proactive service and support for the ACQUITY UPLC system. To use Connections INSIGHT, you must install its service agent software on your workstation. In a client/server system, the service agent must also be installed on the computer from which you control the system.

  • Page 80: Safety And Handling

    Click Submit, and allow approximately 5 minutes to save the service profile. Result: A ZIP file containing your Connections INSIGHT profile is forwarded to Waters customer support for review. Tip: Saving a service profile or plot file from the Instrument Console can require as much as 150 MB of file space.
  • Page 81 Warning: The probe and source can be hot. To avoid burn injuries, take great care while working with these components. Notice: When performing maintenance inside the source enclosure, ensure that the following criteria are met: • Instrument is in Standby mode. •...
  • Page 82 November 16, 2016, 715004521 Rev. C Page 82...

  • Page 83: A Safety Advisories

    Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices.

  • Page 84: Specific Warnings

    A.1.1.2 Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans. Warning:...

  • Page 85: Notices

    A.1.1.3 Biohazard and chemical hazard warning These warnings apply to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials. Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling.

  • Page 86: Warnings That Apply To All Waters Instruments And Devices

    Requirement: Wear clean, chemical-resistant, powder-free gloves when handling samples. Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
  • Page 87 Attention: Manipulez les tubes en polymère sous pression avec precaution: • Portez systématiquement des lunettes de protection lorsque vous vous trouvez à proximité de tubes en polymère pressurisés. • Eteignez toute flamme se trouvant à proximité de l’instrument. • Evitez d'utiliser des tubes sévèrement déformés ou endommagés. •...
  • Page 88 警告：当有压力的情况下使用管线时，小心注意以下几点： • 当接近有压力的聚合物管线时一定要戴防护眼镜。 • 熄灭附近所有的火焰。 • 不要使用已经被压瘪或严重弯曲的管线。 • 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。 경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오. • 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오. • 근처의 화기를 모두 끄십시오. • 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오. • 비금속(Nonmetallic) 튜브를 테트라히드로푸란(Tetrahydrofuran: THF) 또는 농축...

  • Page 89: Warnings That Address The Replacing Of Fuses

    警告： ユ ー ザ ー は、 製造元 に よ り 指定 さ れ て い な い方法で機器 を 使用す る と 、 機器が提供 し て い る 保証が無効 に な る 可能性が あ る こ と に 注意 し て 下 さ い。 Warnings that address the replacing of fuses The following warnings pertain to instruments and devices equipped with user-replaceable fuses.

  • Page 90: Electrical And Handling Symbols

    Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la rubrique "Remplacement des fusibles" du chapitre traitant des procédures de maintenance. Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert im Abschnitt "Sicherungen ersetzen"...

  • Page 91: Handling Symbols

    Symbol Description Safety ground Frame, or chassis, terminal Fuse Functional ground Input Output A.7.2 Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped. Symbol Description Keep upright!
  • Page 92 Symbol Description Upper limit of temperature Lower limit of temperature Temperature limitation November 16, 2016, 715004521 Rev. C Page 92...

  • Page 93: B Specifications

    The specifications presented here depend on the conditions in individual laboratories. Refer ® 2® ® to the ACQUITY System Site Preparation Guide, or contact the Waters Technical Service organization for additional information about specifications. System specifications Table B–1: ACQUITY UPC...
  • Page 94 Table B–3: Electrical specifications Feature Specification Protection class Class I: The insulating scheme used in the instrument to protect from electrical shock. Class I identifies a single level of insulation between live parts (wires) and exposed conductive parts (metal panels), in which the exposed conductive parts are connected to a grounding system.

  • Page 95: Solvent Specifications

    Solvent specifications The following table identifies the recommended co-solvents to use with liquid CO in the ACQUITY system. You can use up to 55% co-solvent with liquid CO . When needed, use up to 5% of acceptable additives in the co-solvent. Also listed are solvents that are not recommended for use in this system.

  • Page 96: Ccbsm Specifications

    Table B–7: ccManager Ceramic PEEK blend Diamond-like coating Flouroelastomer 316 stainless steel Fluoropolymer UHMWPE blend Gold ® Vespel Nitronic 60 Zirconia PEEK Table B–8: CM-30S 316 stainless steel PEEK Diamond-like coating Table B–9: SM-FL Diamond-like coating PEEK Fluoroelastomer Fluoropolymer PPS Alloy Gold 316 stainless steel PEEK blend...
  • Page 97 Table B–10: ccBSM performance specifications (continued) Feature Specification Leak detector Integrated into the drip tray. Pump synchronization Pump cycle synchronizes with the SM-FL injection cycle to enhance retention time reproducibility. Solvent conditioning (co-solvent Integrated vacuum degassing, four chambers. pump only) Gradient formation High pressure mixing, binary gradient.

  • Page 98: Ccmanager Specifications

    Table B–10: ccBSM performance specifications (continued) Feature Specification Composition range 0.0 to 100% of the co-solvent settable in 0.1% increments. co-solvent solubility dependent. Composition precision 0.15% RSD or ± 0.04 min standard deviation, whichever is greater, based on 6 repeat injections. Table B–11: ccBSM Input/Output specifications Attribute...

  • Page 99: Acquity Column Manager With Active Preheating And Auxiliary Column Manager Specifications

    Table B–12: ccManager performance specifications (continued) Feature Specification ABPR sensor Measures system pressure before the ABPR operation ensuring that the pressure does not exceed pressure performance specifications. ABPR dynamic regulating valve Adjusts the system pressure to compensate for thermal changes and solvent-composition changes when delivering pressure gradients.

  • Page 100: Acquity 30-Cm Single-Zone Column Manager Specifications

    Table B–13: CM-A and CM-AUX performance specifications (continued) Feature Specification 4.0 to 90.0 °C, settable in 0.1 °C increments; two Column compartment(or compartments) temperature range independent heat/cool zones per module, up to six zones in a stacked configuration. ±0.5 °C Column compartment(or compartments) temperature accuracy...

  • Page 101: Acquity Upc 2 Photodiode Array Detector Specifications

    ACQUITY UPC Photodiode array detector specifications Table B–15: PDA performance specifications Item Specification ≤ 80 Hz, maximum Data rate Digital filter Variable with data rate Digital resolution 1.2, 2.4, 3.6, 4.8, 6.0, 7.2, 8.4, 9.6, 10.8, 12.0 nm ≤1000 µAU/hr (at 230 nm, 2 Hz, 1 sec TC, 3.6 nm res, dry Drift (dry) analytical flow cell);...
  • Page 102 Table B–16: SM-FL performance specifications Feature Specification Number of sample plates Total of two plates: • 96 and 384 microtiter plates • 48 position 2.00-mL vial plates • 48 position 0.65-mL micro-centrifuge tube plates • 24 position 1.50-mL micro-centrifuge tube plates Maximum sample capacity 768 in two 384-well plates.

  • Page 103: B.10 Physical Specifications

    B.10 Physical specifications Review the physical instrument module specifications and observe all safety advisories. Note: The ACQUITY UPC System requires a rear clearance of at least 15 cm (6 in). Warning: To avoid back injuries, do not attempt to lift the devices without assistance. Table B–18: ccBSM Attribute...
  • Page 104 Table B–22: Attribute Specification Height 19.3 cm (7.6 inches) Width 34.3 cm (13.5 inches) Depth 60.7 cm (23.9 inches) Weight 15.9 kg (35.0 pounds) Table B–23: SM-FL physical specifications Attribute Specification Height 27.1 cm (10.7 inches) Width 34.3 cm (13.6 inches) Depth 71.2 cm (28.0 inches) Weight...

  • Page 105: C Configuring The Core 4 System Variant

    Configuring the core 4 system variant The UPC core 4 configuration variant has two 30-cm single-zone column managers instead of one. Because of this, it is frequently referred to as a dual CM-30S with primary and secondary ovens. Physical arrangement Figure C–1: Example of the core 4 configuration system variant Solvent...

  • Page 106: Column Configuration

    Column configuration Figure C–2: Dual CM-30S supporting 15 columns with bypass and waste omitted Configuring software for dual-column managers Column manager software must be configured to define the path of the solvents and samples as they flow through the inlet and outlet valves. The following procedure applies only to UPC systems with two CM-30S modules running Empower software.
  • Page 107 To configure the chromatographic system: In Empower software, start the system configuration procedure. On the Configuration tab, drag and drop the name for the primary column manager from the Available Components pane to the System Configuration pane. On the Configuration tab, drag and drop the name for the secondary column manager to the System Configuration pane.
  • Page 108 See also: For detailed instructions, see the console online Help. Recommendation: Reboot the workstation after configuring software for a column manager. Result: After completing this procedure, when you select each column manager in the console system tree, the columns appear. The primary column manager is listed first with Columns 1 through 7 available.
  • Page 109 Restriction: Do not select the Bypass position on the primary CM-30S. In a dual configuration, the bypass port is used to direct flow from the outlet of the primary column manager to the inlet of the secondary column manager. The Bypass selection is therefore invalid, and selecting Bypass will result in an alarm.
  • Page 110 Table C–1: “Multi mode column selection is out of range” alarms Column Selection Situation Resolution Instrument method You selected Bypass in one Replace Bypass with None. column manager and a column Control panel number or No Change in the Console other column manger.

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