Histogram Flags - SYSMEX pocH-100i Instructions For Use Manual

2.1.8. Histogram flags

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The pocH-100 extracts the characteristics of the histogram and displays them as histogram flags.
If there are histogram flags, repeat analysis. If flags are still displayed, one of the following problems may apply.
Flag Probable sample cause
Incomplete lysing of red blood cells, presence of nucleated red blood cells, in-
WL
crease in large platelets, platelet aggregation or agglutination, precipitation of
fibrin, presence of proteins or lipids.
Presence of fragmented red blood cells, increase in large platelets, platelet aggre-
RL
gation or agglutination, presence of micro-erythrocytes.
Effects of cryoglobulins, fragmented red blood cells, or cellular fragments of white
PL
blood cells.
Incomplete lysing of red blood cells, presence of immature white blood cells,
WU
white blood cell aggregation, platelet satellite phenomenon, etc.
RU Effects of cold agglutinin, inclusion of white blood cells.
Increase of large platelets, inclusion of fragmented red blood cells, precipitation
PU
of cryoglobulins, platelet aggregation or agglutination, presence of micro-erythro-
cytes.
DW (RBC) Significant anisocytosis, etc.
Inclusion of fragmented red blood cells, nonuniformity in size of platelets, effects
DW (PLT)
of cryoglobulins, etc.
Effects of anemia treatment or blood transfusion causing the presence of cells of
MP (RBC)
multiple sizes.
MP (PLT) Platelet aggregation, low platelet count.
Incomplete lysing of red blood cells, etc., causing the first two WBC populations
T1
in the WBC-Histogram not to be separated, presence of CML or other immature
granulocytes.
Aged sample, incomplete lysing of red blood cells, etc., causing the last two WBC
T2
populations in the WBC-Histogram not to be separated, presence of CML or other
immature granulocytes.
Sample with high values for monocytes, eosinophils, and basophils, incomplete
F1, F2, F3
lysing of red blood cells, aged sample, etc., presence of CML or other immature
granulocytes.
Presence of nucleated red blood cells, increase of large platelets, platelet aggrega-
AG
tion or agglutination, precipitation of fibrin, presence of proteins or lipids, etc.
Correction
Warm sample Wash
Check Manual
and repeat blood
smear. count.
analysis. cells.
If incomplete lysing is suspected, perform a 1:5 dilution of the
sample (50 μL of whole blood added to 200 μL of diluent) and
re-analyze. Adjust the results for the dilution factor.
If incomplete lysing is suspected, perform a 1:5 dilution of the
sample (50 μL of whole blood added to 200 μL of diluent) and
re-analyze. Adjust the results for the dilution factor.
If cyroglobulins are suspected, first warm the sample and
repeat analysis. If error message persists, perform a plasma
replacement (remove plasma and replace with equal volume
of diluent) and repeat analysis.
If incomplete lysing is suspected, perform a 1:5 dilution of the
sample (50 μL of whole blood added to 200 μL of diluent) and
re-analyze. Adjust the results for the dilution factor.
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pocH-100 Revised February 2011
2 -12