System Limitations - Interferences - SYSMEX pocH-100i Instructions For Use Manual

WBC
RBC
Carry-over HGB
HCT
PLT
Reagents:
Consumables Detergent:
Control material: EIGHTCHECK-3WP
in whole blood mode:
Aspirated sample volume
in pre-diluted mode:
Number of analyses that can be per- pocH-pack 65:
formed with 1 pocH-pack 65/pocH-pack pocH-pack 65XL: Approximately 240
65XL (These numbers are cycle counts.)
1.2.2. System limitations – Interferences
Lyse resistant erythrocytes
Cold agglutinins / cryoglobulins Increased MCV, increased MCHC due to decreased HCT and RBC
WBC: false high leukocyte count
Platelet aggregation
Nucleated erythrocytes
Cold agglutinins
RBC: false low erythrocyte count Microcytosis (severe)
Fragmented erythrocytes
Lipaemia
HGB: false high haemoglobin measure-
Abnormal protein
ment
Leukocytosis
Cold agglutinins
HCT: false low haematocrit measurement
Fragmented erythrocytes
HCT: false high haematocrit measurement Leukocytosis
Platelet aggregation
PLT: false low platelet count
Giant platelets
Microcytic erythrocytes
PLT: false high platelet count
Fragmented erythrocytes
3% or less
1.5% or less
1.5% or less
1.5% or less
5% or less
pocH-pack 65/pocH-pack 65XL
CELLCLEAN
approx. 15 μL
approx. 200 μL of the diluted sample; a minimum of 20 μL capillary blood is required.
Approximately 65
Cause
Abnormal WBC histogram (WL flag)
Abnormal WBC histogram (WL flag) and Abnormal PLT histogram (PU flag)
Abnormal WBC histogram (WL flag)
Increased MCV, increased MCHC due to decreased HCT
Low MCV
Abnormal RBC histogram (RL flag) and Abnormal PLT histogram (PU flag)
MCHC > 36.5 g/dL in severe cases
MCHC > 36.5 g/dL in severe cases
WBC >100,000/μL
Increased MCV and increased MCHC
Abnormal RBC histogram (RL flag) and abnormal PLT histogram (PU flag)
Very high leukocyte count with low erythrocyte count present
Abnormal PLT histogram (PU flag)
Abnormal PLT histogram (PU flag)
Low MCV
Abnormal RBC histogram (RL flag) and abnormal PLT histogram (PU flag)
Potential Detection
Note
The abnormal sample conditions listed
here are known to affect test results.
The majority of the listed sample condi-
tions are not measured quantitatively
because these conditions vary due to
patient population, patient diagnosis,
age, medications, etc. Customers can
perform studies in order to show how
their specific patient populations are af-
fected by various conditions.
i
pocH-100 Revised February 2011
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