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Table of Contents
Related Manuals for SYSMEX CyFlow Ploidy Analyser
Summary of Contents for SYSMEX CyFlow Ploidy Analyser
- Page 1 ® CyFlow Ploidy Analyser Instrument Operating Manual Revision: Rev-03_2014-10-22 Issued by: Sysmex Partec GmbH Sysmex Partec GmbH Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
- Page 2 Phone: +49 (0) 3581 8746 0 Fax: +49 (0) 3581 8746 70 Mail: support@sysmex-partec.com Web: www.sysmex-partec.com Sysmex Partec GmbH 2 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 3: Table Of Contents
Intermediate cleaning process ..............26 Sheath and Waste bottle ................27 Measurement parameters .................. 27 Sysmex Partec GmbH 3 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 4 Ploidy Analyser Optics ..............45 ® 10.4.6 CyFlow Ploidy Analyser Fluidics ..............45 Notes ......................... 46 Sysmex Partec GmbH 4 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 5: Introduction
® Microsoft Corp.: Windows, Word, Excel, PowerPoint, Paint. ® Hewlett Packard : Deskjet Laserjet. Sysmex Partec GmbH 5 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 6: Presentation
(e.g. Howard M. Shapiro, Practical Flow Cytometry. Wiley 2002). Sysmex Partec GmbH 6 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 7: Introduction To Flow Cytometry
Sysmex Partec GmbH 7 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 8: Optical Standard Setup - Parameters And Filters
Optical filtration is used to adapt the wavelength reaching the optical detector to the emission wavelengths of the used fluorochrome. Sysmex Partec GmbH 8 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 9 P and T reagent kits, intercalates into the double-stranded DNA and is excited by green light (Nd-YAG laser). Propidium iodide emits orange and red Sysmex Partec GmbH 9 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 10: In Flow Cytometry, What Is
To exclude noise from a histogram already acquired, a region-gate can be used. Sysmex Partec GmbH 10 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 11: Instrument Starting Procedure
Once the Ploidy Analyser started no further steps are necessary. You can On/Standby directly start your measurements! Sysmex Partec GmbH 11 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 12 Login: USER (case sensitive) Password: Cube1 (case sensitive) Sysmex Partec GmbH 12 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 13: User Levels
To enter the CyView login with your personal Login name followed by “Work with CyView™. Sysmex Partec GmbH 13 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 14: Cyview Tm Controls
Instrument real time display of workload (L), on-board memory status (M), analysis volume (V) and analysis duration (D). Sysmex Partec GmbH 14 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 15: Cyview™ Instrument Setting Controls
Select CR – Mode to show “All events” or “Regions only” Confirm all modifications by pressing Accept Sysmex Partec GmbH 15 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 16: Results
Confirm all modifications by pressing Accept Result will be display in the “Results table” next to the “Region statistics” Sysmex Partec GmbH 16 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 17: Regions
Use arrow keys to switch between regions Confirm all modifications by pressing Accept Sysmex Partec GmbH 17 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 18: General - Properties Of The Instrument
µl/s (for regular analysis between 0.2 and 4 µl/s) Lights on switches light sources on / off Sysmex Partec GmbH 18 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 19: Measure - Definition Of Measure Modes
Automatic storage at the end of the measurement. Autosave does not function when the measurement is finished by the “end” button. Sysmex Partec GmbH 19 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 20: Measure Modes
Volumetric counting (VC) of volume Volumetric counting (VC) electrodes Cells in region Sysmex Partec GmbH 20 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 21 STOP condition (select the respective MaxCount function in the REGIONS register). Sysmex Partec GmbH 21 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
- Page 22 Schematic overview of the measure modes with a flash indicates clearing of the data. User selectable criteria in green, software-defined values in red Sysmex Partec GmbH 22 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 23: Cyview Tm Instrument/Measurement Settings
These settings are crucial for a correct verification of the instrument performance. Initialization batch, Work batch and Clean batch Sysmex Partec GmbH 23 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 24 At the end press “Continue”. The instrument will be cleaned and the priming process ends. Switch to “WORK” batch Sysmex Partec GmbH 24 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 25: Work Process
4010) and press “Continue” Connect a sample tube with sheath fluid (Order No.04-4007) Sysmex Partec GmbH 25 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 26: Intermediate Cleaning Process
Clean-symbol display in the upper part, if this symbol will be select the complete shutdown process will be performed. Sysmex Partec GmbH 26 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 27: Sheath And Waste Bottle
A higher speed can be used when the particle concentration is measured and accuracy is of lower relevance Sysmex Partec GmbH 27 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 28: Light Source (L)
This option allows selecting the part of the data to be displayed: All events Region only Color gating Sysmex Partec GmbH 28 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 29: Region/Roi Properties
To set the ROI/region as a sorting gate, just by selecting the box. (This option is not applicable on the Ploidy Analyser). Sysmex Partec GmbH 29 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 30 Sysmex Partec GmbH 30 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 31: Compensation
The function of compensation is not applicable to the use of the instrument for DNA analysis. Sysmex Partec GmbH 31 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 32: Typical Sample Analysis
Prerun and stabilization of sample flow Start Measure Data acquisition Console displaying control board status Sysmex Partec GmbH 32 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 33 A volume can be pre-selected Cells in region o A particle number can be pre-selected Sysmex Partec GmbH 33 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 34: Automatic Peak Analysis
Measure) the selected peak analysis mode (CV Peak- Analyze / GAUSS Peak-Analyze) will be automatically triggered after a measurement is started. Sysmex Partec GmbH 34 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 35 Median – median value of signals Left / Right – left and right margin of the peak area Sysmex Partec GmbH 35 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 36: Service/Administrator Mode
StartEnd Search Limit_startend – amplitude relation in % to search start- and endpoints of the peak Sysmex Partec GmbH 36 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 37: Export Of Peak Analysis And 1-Parameter Histograms
A regions section with the results of the peak analysis Sysmex Partec GmbH 37 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... - Page 38 Version 3: Exporting png files as well as histogram tables. Sysmex Partec GmbH 38 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com...
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Page 39: Keyboard/Mouse Combinations
Marks the region and moves it in all regionplots Region Central mouse in the visual area Sysmex Partec GmbH 39 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 40: Appendix
PPE (personal protection equipment) must be met for all users. Therefore, the system is marked with the following biohazard label: Warning: biohazards Sysmex Partec GmbH 40 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 41: Maintenance
Instrument and software support News and events And much more ... www.sysmex-partec.com Sysmex Partec GmbH 41 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 42: Transport And Storage
After decontamination, the system has to be disposed according to the local regulations and laws. For further information, please contact your local distributor or Sysmex Partec. Sysmex Partec GmbH 42 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 43: Laser Safety
Laser radiation Class IIIb, if cover is removed and shutter is opened Additional Explanation Sysmex Partec GmbH 43 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 44: Technical Specifications
Cleaning Solution (Sysmex Partec No. 04-4009) Decontamination Solution (Sysmex Partec No. 04-4010) Hypochlorite 0.5% (Sysmex Partec No. 04-4012) Sysmex Partec GmbH 44 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 45: Cyflow ® Ploidy Analyser Optics
Completely closed system for sheath water and sample volumes; no fluid droplets or aerosols generated nor released from the instrument. Sysmex Partec GmbH 45 / 47 Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com · www.sysmex-partec.com... -
Page 46: Notes
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