SYSMEX pocH-100i Instructions For Use Manual page 6

INTRODUCTION
WARRANTY
All Sysmex instruments are warranted against defective material or workmanship for a
period of one year, commencing on the installation date on the customer's premises
This warranty does not cover any defect, malfunction or damage due to:
• Accident, neglect or willful mistreatment of the product;
• Failure to use, operate, service or maintain the product in accordance with the applica-
ble Sysmex Instructions for Use;
• Failure to use the appropriate reagents and consumables specified for the product.
3 i
pocH-100 Revised February 2011
FUNCTIONAL DESCRIPTION
ANALYSIS
An exact volume of sample is aspirated with the aspirating piercer (pipette).
This volume of the sample is then transferred (together with a defined volume of dilu-
ent) to the mixing chamber and from there automatically to the transducer.
All parameters are analyzed using the same transducer in the order:
(1) WBC/HGB and (2) RBC/PLT.
For WBC/HGB analysis, the WBC/HGB lyse is added to the measuring chamber to pro-
vide further dilution and hemolysis of the sample's RBC.
This process takes about ten seconds.
During this reaction period, the erythrocytes are dissolved under the influence of the
lysis, hemoglobin is released and is converted into red methemoglobin. The leukocytes
remain intact.
The volume and number of the leukocytes (WBC) are determined by the DC detection
method. In the HGB detector, the hemoglobin concentration is photometrically meas-
ured.
For RBC/PLT analysis, the diluted sample is transferred from the mixing chamber to the
transducer, and the volume and blood cell count for erythrocytes and platelets are ana-
lyzed by the DC detection method.
TECHNICAL BASICS
• The calculation of indices is based on international principles in hematology.
• The method for counting blood cells is based on the electric resistance detection prin-
ciple.
• Hemoglobin concentration is determined by a photometric measuring method.