Absolute Cell Counting; Figure 3: Fluorescence Signals Are Displayed And Analysed In Histogram And/Or Dot Plot Diagrams - SYSMEX CyFlow Space Operating Manual

histograms show the number of cells within the channels. Two parameter dot plots show
the correlation between two cell properties.

Figure 3: Fluorescence signals are displayed and analysed in histogram and/or dot plot diagrams

3.4

Absolute Cell Counting

Since the CyFlow™ Space analyses all cells passing through the flow cell while precisely
monitoring the fluid volume of the sample, it allows volumetric counting during the analysis,
i.e. the determination of concentration of any cell subpopulation. Cell subpopulations can
even be defined and their concentration analysed at a later date, after reloading data from
a file.
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CyFlow Space | Operating Manual | March 2021