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SYSMEX SF-3000 Operator's Manual

SYSMEX SF-3000 Operator's Manual

Automated hematology analyzer
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Table of Contents

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Copyright © 1995 - 2001 by SYSMEX CORPORATION
All rights reserved. No part of this Operator's Manual may be
reproduced in any form or by any means whatsoever without
prior written permission of SYSMEX CORPORATION.
SF-3000/06.95
OPERATOR'S MANUAL
AUTOMATED HEMATOLOGY ANALYZER
SF-3000
SYSMEX CORPORATION
KOBE, JAPAN
Code No. 461-2459-3
PRINTED IN JAPAN
Date of Last Revision: March 2001

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Summary of Contents for SYSMEX SF-3000

  • Page 1 AUTOMATED HEMATOLOGY ANALYZER SF-3000 SYSMEX CORPORATION KOBE, JAPAN Copyright © 1995 - 2001 by SYSMEX CORPORATION All rights reserved. No part of this Operator’s Manual may be Code No. 461-2459-3 reproduced in any form or by any means whatsoever without PRINTED IN JAPAN prior written permission of SYSMEX CORPORATION.
  • Page 2 Sysmex is a registered trademark of SYSMEX CORPORATION. CELLPACK, CELLCLEAN, STROMATOLYSER, SULFOLYSER, SF CHECK are trademarks of SYSMEX CORPORATION. Canon is a registered trademark of Canon, Inc. Cubitainer is a registered trademark of Hedwin Corporation. DeskJet and LaserJet are registered trademarks of Hewlett-Packard Corporation.
  • Page 3 RECEIVING INSTRUCTIONS The SF-3000 has been thoroughly tested before shipment, and has been packaged carefully to prevent damage from shipping and handling. Reagents and options have also been sent and will arrive at approximately the same time as the analyzer. Follow these guidelines when the system arrives: •...
  • Page 4 QC Analysis : Manual Mode ..........2-7 QC Analysis : Auto Mode ........... 2-12 QC Analysis : Manual Closed Mode ........2-13 QC Analysis : Manual CP Mode ......... 2-17 (To be continued) Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 5 Select By Flagging .............. 4-27 Select By Date..............4-29 Select By No Output............4-31 QC Data................4-33 All Samples ................. 4-34 Read FD................4-35 (To be continued) Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 6 Clean RBC Detector Aperture ..........5-38 Execute Remove Flow Cell Air Bubbles Sequence .... 5-41 Execute Replace Sheath Flow Reagent Sequence ....5-43 Replace Waste Container (if used) ........5-45 (To be continued) Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 7 X M Control............... 6-48 11. VARIABLE TARGET FUNCTION ............6-51 11.1 How Target Values Are Calculated........6-51 11.2 Variable Target Function and Erroneous Data ....6-52 12. CONTROL CHART DEFINITION ............6-53 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 8 3.10 Test LCD Display ..............8-78 3.11 Test Touch Panel ..............8-79 3.12 Test Output ................8-80 3.13 Version Check ..............8-83 3.14 Service Data ................. 8-85 STATUS DISPLAY ................8-90 ERROR LOG ..................8-94 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 9 MAIN UNIT ELECTRONIC SYSTEM ..........10-16 MAIN UNIT COMPONENTS............. 10-18 Front................... 10-18 Rear ..................10-20 Right Side ................10-22 Left Side................10-24 Front Interior..............10-25 Left Interior................ 10-26 Right Interior ..............10-30 (To be continued) Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 10 Normal Range Display ............11-49 Date Format ............... 11-51 ID Reader ................11-52 Blood Sensor..............11-54 Work List ................11-56 OUTPUT SET VALUES ..............11-57 7.1 Output to GP............... 11-57 7.2 Backup to FD..............11-57 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 11 10.2 Pneumatic Cable Connections ..........B-30 10.3 Power Cord Connections ............ B-30 11. POWER ON AND SYSTEM SETUP........... B-31 11.1 Power On ................B-31 11.2 System Setup ..............B-31 11.3 System Check ..............B-32 viii Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 12 Applicable Bar Codes ............C-17 Dimensions of Elements ............. C-18 Requirements on Wide/Narrow Ratio ........ C-18 Optical Requirements ............C-18 Dimensions of Bar Code Label........... C-20 Check-Digit................. C-20 Applicable Characters............C-28 Effective Bar Code Length ..........C-28 Sysmex SF-3000 Operator's Manual -- Revised September1995...
  • Page 13 CHAPTER 1 INTRODUCTION INTRODUCTION ............... 1-1 OPERATOR'S MANUAL ORGANIZATION ....... 1-2 INFORMATIONAL HEADINGS ..........1-3 3.1 NOTE, CAUTION and WARNING........1-3 3.2 Document Conventions ........... 1-3 SYSTEM OVERVIEW ............1-4 SYSTEM OPTIONS............. 1-5 SAFETY ................ 1-6 6.1 General Safety ............. 1-6 6.2 Hazardous and Biohazardous Material.........
  • Page 14 In addition, it can contribute in the diagnosis and therapeutic monitoring of patients. The SF-3000 has the capability of analyzing up to 80 samples per hour, and display 4 different patterns -- WBC 5 differential, scattergrams for white blood cells and basophils, and cell size distribution curves for red blood cells and platelets, as well as 23 analysis data parameters on the color LCD (liquid crystal display) screen.
  • Page 15 INTRODUCTION OPERATOR'S MANUAL ORGANIZATION The SF-3000 Operator's Manual has been designed with the operator in mind. Each chapter has its own table of contents and is designed to facilitate rapid location of appropriate information. Table 1-1: Manual Organization CHAPTER 1 Contains basic information about the instrument;...
  • Page 16 "All Samples", "Ready" or "Input Password" [Square Brackets]: Names of function keys such as control or operational keys, number keys, or arrow keys that you select on the SF-3000 appear within square brackets. For example: [Enter], [Sampler], [2] or [↑] Italics: Application names and reference material appear in italic form.
  • Page 17 INTRODUCTION SYSTEM OVERVIEW The purpose of the SF-3000 is to provide accurate results on 23 parameters and to detect abnormalities in the sample. To assist the laboratory in the screening of abnormalities, the SF-3000 analyzes and processes the data obtained from the analyzer unit, and the LCD displays and outputs either a POSITIVE or a NEGATIVE message for each sample.
  • Page 18 5 sample racks (50 sample tubes) that enable an automated closed sampling system. • Manual CP Unit : Consists of a mixer and a cap piercer unit which enables a manual closed sampling system. Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 19 The operator must observe all NOTES, CAUTIONS and WARNINGS posted anywhere on the instrument or given in this manual. Do NOT touch the electrical circuits behind the enclosure panels of the SF-3000. Touching the circuits is dangerous and may give you an electrical shock, especially if your hands are wet.
  • Page 20 INTRODUCTION, Safety 6 . 4 Laser Safety The SF-3000 uses a semiconductor laser unit. Though this laser unit is placed in the shield box, the operator must observe all NOTES, CAUTIONS and WARNINGS concerning laser handling. WARNING!: DO NOT remove the shield box cover of the Laser Unit.
  • Page 21 INTRODUCTION OPERATIONAL SUMMARY The SF-3000 has two basic operating modes: the Manual Open Mode and Capillary Mode. Additional three operating modes are available by means of optional units: the Sampler Mode and Manual Closed Mode by means of Cap Piercing Sampler Unit, and Manual CP Mode by means of Manual CP Unit.
  • Page 22 INTRODUCTION ANALYSIS PARAMETERS The SF-3000 uses 4 detection methods and 5 types of reagents to analyze the following 23 parameters: Table 1-3: Analysis Parameters Parameter Acronym Detection Method White Blood Cell Count Flow Cytometry by Semiconductor Laser Red Blood Cell Count...
  • Page 23 This area displays Main Unit status information such as analysis status, errors, etc. It also displays data processing status information such as Peripherals, X , etc. The [Sysmex] and [Sampler] function keys also appear in this area. Ready Manual Mode Next No.1234567890123...
  • Page 24 DP No. If 2 or more errors occur simultaneously, they are displayed in order of importance. When an error is displayed, press [Sysmex] key to display the action/help messages provided in the Error Log program. Refer to Chapter 8: Troubleshooting for the action message display.
  • Page 25 The [Sysmex] and [Sampler] keypads appear at each end of this area. [Sysmex] -- Press this key to obtain the hard copy of screen and to access the Error Log. Refer to Section 9.3 in this chapter for hard copy output. Refer to Chapter 8: Troubleshooting for Error Log.
  • Page 26 The LCD contrast will automatically decrease for the LCD power saving if the Main Unit does not detect any key operation on the LCD for 5 minutes. Touch anywhere on the LCD to exit the power saving mode. Sysmex SF-3000 Operator's Manual -- Revised September 1995 1-13...
  • Page 27 By pressing the [Return] key on any screen, the visible display returns to the previous screen, finally to the Main Screen. The SF-3000 provides twelve Root Menus. The Main Screen displays seven Root Menus on the menu line at a time. When you want to change a menu, press the [More] key to alternate root menu.
  • Page 28 Use this menu to calibrate the SF-3000 for specified parameter(s) automatically or manually. Refer to Chapter 7: Calibration for the procedure. [Shutdown] Use this menu to clean the detector chambers and all hydraulic lines in the SF-3000 system with detergent. [Auto Rinse] Use this menu to rinse the hydraulic lines with the diluents, and check the background values after rinsing.
  • Page 29 INTRODUCTION 9 . 3 Hard Copy The [Sysmex] keypad always appears on the left of the top line of the screen. Press this key to display the Sysmex submenu window as shown in Figure 1-6. Ready Manual Mode Next No.1234567890123...
  • Page 30 Enter Cancel Figure 1-7: Password Entry Screen The password is important for the management of the SF-3000, so be sure to memorize it and/or have aids to help you remember it. Should you forget it, consult your Sysmex service representative.
  • Page 31 Refer to Chapter 5: Maintenance and Supplies for the procedures. 1 2 . ALARMS In the SF-3000, 3 types of alarm sounds are used to alert the operator: 1. Key Entry A short beep (approx. 0.1 sec.) sounds every time a key is pressed on the LCD touch panel keypad.
  • Page 32 Failure to properly ground the SF-3000 bypasses important safety features and may result in an electrical hazard. The power source required for the SF-3000 system is: 117, 220 or 240 VAC ± 10%, 50/60 Hz. The power consumption of the system, including the Pneumatic Unit and Sampler Unit, is 600 VA or less.
  • Page 33 Installation Environment The environmental requirements are listed below: • Operate the SF-3000 within a temperature range of 15 - 30°C (optimum temperature 25°C) and relative humidity of 30 - 85%. • To compensate for heat generated by the system, approximately 430 kcal/hour (1705 BTU/hour) is required in an air-conditioned environment.
  • Page 34 : for 13 QC files Quality Control X Control (or L-J Control) : 180 points x 12 files, 28 parameters X M Control : 180 points x 1 file, 28 parameters Sysmex SF-3000 Operator's Manual -- Revised September 1995 1-21...
  • Page 35 ± 2% or within 0.03 x 10 for Closed mode /µL within ± 5% or within 10 x 10 for Open mode /µL within ± 10% Accuracy within ± 8% for Capillary mode within ± 12% 1-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 36 /µL) NOTE: The Linearity range is different from the Display range. Consult the Linearity specification for the reportable range for each parameter. The Display range is given on the next page. Sysmex SF-3000 Operator's Manual -- Revised September 1995 1-23...
  • Page 37 Interleaved 2 of 5, JAN Classification Type of protection against electric shock Class I equipment Degree of protection against electric shock Type B applied part Heat Compensation Required Approx. 2047 BTU/h (516 kcal/h) 1-24 Sysmex SF-3000 Operator's Manual -- Revised March 2001...

  • Page 38: Table Of Contents

    CHAPTER 2 SAMPLE PROCESSING INTRODUCTION ...............2-1 1.1 Summary of Operation Mode ..........2-1 1.2 Operational Procedures in Each Mode ........2-2 START-UP PROCEDURE .............2-3 2.1 Operator Checks ............2-3 2.2 Turning On the Power ............2-4 2.3 Self-Checks ..............2-4 2.4 Pressure Gauge Check............2-6 2.5 Auto Output Settings Check..........2-6 QUALITY CONTROL ANALYSIS ...........2-7 3.1 QC Analysis : Manual Mode ..........2-7 3.2 QC Analysis : Auto Mode ..........

  • Page 39: Introduction

    SAMPLE PROCESSING INTRODUCTION If your SF-3000 is equipped with the auto sampler unit (optional), analyses can be performed in four modes: manual open, capillary, auto (sampler), manual closed. If your SF-3000 is equipped with the manual CP unit (optional), analyses can be performed in three modes: manual open, capillary, manual CP mode.

  • Page 40: Operational Procedures In Each Mode

    Operations performed by the operator are indicated in bold print. Ready: READY LED turns on and "Ready" message is displayed on the LCD screen. In this status, various operations can be executed, including analysis setting and data processing. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 41: Start-Up Procedure

    Make sure that the left and right rack pools and analysis line are clear. Waste Fluid Discharge any waste fluid that has been collected in the pneumatic unit drain trap and waste container (if applicable). Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 42: Turning On The Power

    If any error occurs during the self-check, an error message appears on the screen. Jot down the message and turn the power OFF and ON again. If the same error message appears, contact a Sysmex service representative.
  • Page 43 Press [More] key to display the [Auto Rinse] key. Press the [Auto Rinse] key to perform a background check again. If the parameters are still not within the acceptable range, see Chapter 8, Troubleshooting. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 44: Pressure Gauge Check

    If Auto Output to the peripheral printer(s) and/or a host computer is desired, before starting analysis, make sure that the instrument is set to automatically transmit/print. See Chapter 11: System Setup. Sysmex SF-3000 Operator's Manual -- Revised July 1998 Sysmex SF-3000 Operator’s Manual - - Revised September 1995...

  • Page 45: Quality Control Analysis

    Manual Mode Capillary Mode Closed Mode ENTER Ð CANCEL Figure 2-2: Sample No. Setting Screen The selected analysis mode is backlit in light-blue. Press [Manual Mode] to select Manual mode, if needed. Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 46 When the [Return] key is pressed, the program ends and the control data is automatically accepted and plotted. NOTE: Press the [Graphic] key on the QC Data Display screen to display the graphic display. Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 47 "Mean" column. Refer to Figure 2-5a. The sample No. on the graphic screen is indicated as "QC01" (see Figure 2-5b). NOTE: Two analysis results obtained consecutively without any abnormality are used for the X Control. Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 48 [fL] PLT Flag RDW-CV 15.7 [ % ] [x10 /uL] 250fL 40fL [fL] 10.3 [fL] P-LCR 24.3 [ % ] QC Data Return Control Data Graphic Display (Manual Mode) Figure 2-5b: 2-10 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 49 Reanalyze the sample. If an analysis error occurs during QC analysis, the error message <QC Analysis Error> will be displayed. The results are not plotted as a control data point. Reanalyze the sample. Sysmex SF-3000 Operator's Manual -- Revised December 1995 2-11...

  • Page 50: Qc Analysis: Auto Mode

    SF CHECK is analyzed and the lot has already been expired. The analyzed data will not be plotted on the QC chart. Refer to Chapter 6, Section 9: Sampler QC Analysis for further information. 2-12 Sysmex SF-3000 Operator's Manual -- Revised December 1995...

  • Page 51: Qc Analysis: Manual Closed Mode

    QC file. Refer to Chapter 6, Section 6.6: Lot Information for the procedure. Press the [ENTER] key. The QC file No. is specified and the QC Data Display will appear as shown in Figure 2-8. Sysmex SF-3000 Operator's Manual -- Revised December 1995 2-13...
  • Page 52 10.3 MCHC 33.3 MONO% RDW-SD 46.0 RDW-CV 15.3 BASO% 177.4 GRAN-X GRAN-Y 177.3 44.2 10.0 WBC/BA-X 94.8 P-LCR 23.7 WBC/BA-Y GRAN-Y(W) 43.0 Graphic Return Figure 2-9a: "X1" Data Display (Closed Mode) 2-14 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 53 177.1 177.2 44.2 44.1 44.2 10.0 10.6 10.3 WBC/BA-X 94.8 94.6 94.7 P-LCR 23.7 24.9 24.3 WBC/BA-Y GRAN-Y(W) 43.0 43.0 43.0 Graphic Return Figure 2-9b: QC Data Display Screen (Closed Mode) Sysmex SF-3000 Operator's Manual -- Revised December 1995 2-15...
  • Page 54 No. as "QC03" and re-analyze the control. The data in the "Data" column will be replaced with the newly obtained results, and the new data will be handled as the L-J Control data. 2-16 Sysmex SF-3000 Operator's Manual -- Revised December 1995...

  • Page 55: Qc Analysis: Manual Cp Mode

    Manual Closed mode. Refer to the previous Section 3.3: QC Analysis: Manual Closed Mode for the detailed procedures. To analyze the control material in the Manual CP mode, see Section 4.5: Manual CP Mode in this chapter. Sysmex SF-3000 Operator's Manual -- Revised December 1995 2-17...

  • Page 56: Analysis Mode Procedures

    • Samples must be warmed to room temperature for approximately 30 minutes before analysis. The height of the blood sample tube should be 75 mm or shorter. Sample volumes are shown below. Approx. 170 µL Aspirated sample volume: 2-18 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 57 Press [ENTER]. The next sample No. is set and the Sample No. Setting screen will disappear. The input sample No. will be displayed in the Next No. column in the top line of the LCD. Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-19...
  • Page 58 • To remove the sample, pull straight down to prevent bending the manual aspiration pipette. • The manual aspiration pipette is automatically rinsed, and does not need to be wiped manually. 2-20 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 59 W B C < 1.00 x 10 /µL RBC < 0.30 x 10 /µL HGB < g/dL < x 10 /µL Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-21...
  • Page 60 NOTE: If the [Auto Rinse] key is not displayed on the LCD, press [More] key to change the root menu display. 2-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 61: Capillary Mode

    The height of the blood sample tube should be 75 mm or shorter. Sample volumes are shown below. 40 µL or more Required sample volume: Approx. 170 µL Diluted sample volume aspirated: Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-23...
  • Page 62 When the next sample No. is not set, or after the power ON, the next sample No. is automatically set to No. 1, increasing by 1 for each succeeding sample (e.g., 1, 2, 3, ...). 2-24 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 63 Also, if diluent is dispensed ahead of time, measurement errors are generated because of evaporation and contaminants; therefore, prepare new diluted sample each time an analysis is performed. Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-25...
  • Page 64 When the READY LED turns on again, "Sample Asp Ready" is displayed. Prepare the next sample and repeat steps (1)-(3) above. NOTE: If sample ID numbers are not set, they are automatically assigned sequentially. 2-26 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 65 [ % ] [x10 /uL] 250fL 40fL [fL] 10.3 [fL] P-LCR 24.3 [ % ] Work Stored Replace Status Return List Data P r i n t P r i n t Reagent Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-27...
  • Page 66 Press the Next No. keypad in the top line of the LCD. The Sample No. Setting screen will appear. Press the [Manual Mode] key to select Manual mode, and press the [Enter] key. 2-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 67: Auto Mode

    Use the type of blood collection tube shown below for auto mode analyses. Diameter a: 12 - 15 mm Length b: 75 mm Overall length c: 82 mm or less Figure 2-19: Tube Dimensions Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-29...
  • Page 68 Affix the rack label between the first and the second tubes, aligning to the top of the rack. Figure 2-21: Affix Sample Tube Figure 2-22: Affix Rack Bar code Bar Code Label Label 2-30 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 69 Start Check window will appear. Ready Manual Mode Next No.1234567890123 Sysmex Sampler DP:1234567890123 XM HC GP DP SU Sampler Start check 1234 Rack Tube Sample 123456 Cancel Start Figure 2-23: Sampler Start Check Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-31...
  • Page 70 • A number will not be carried beyond a hyphen. For example, the number following 2-999 will be 2-000; 1-99-000 will follow 1-99-999. • The number following 9999999999999 will be 1. 2-32 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 71 • The blood sample tube position number (Tube Pos. No.) shows the location of each tube in the rack. Tube Position No. 9 10 TUBE position No. Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-33...
  • Page 72 DIFF Count RBC Analyze WBC/BASO Analyze DIFF Analyze Edit Store : Entry : Run : Finish Normal : Interruption : Finish Abnormal ← → Return Figure 2-26: Analysis Process Screen (Auto Mode) 2-34 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 73 [fL] P-LCR 24.3 [ % ] Work Stored Replace Status Return List Data P r i n t P r i n t Reagent Figure 2-27: Analysis Results Screen (Auto Mode) Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-35...
  • Page 74 NOTE: If the [Auto Rinse] key is not displayed on the LCD, press [More] key to change the root menu display. 2-36 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 75: Manual Closed Mode

    Use the type of blood collection tube shown below for Manual Closed analysis. Diameter a: 12 - 15 mm Length b: 75 mm Overall length c: 82 mm or less Figure 2-28: Tube Dimensions Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-37...
  • Page 76 Sysmex Sampler DP:1234567890123 XM HC GP DP SU Analysis Mode Closed Sample Number 1234567890123 Manual Mode Capillary Mode Closed Mode Ð ENTER CANCEL Figure 2-29: Sample No. Setting Screen (Closed Mode) 2-38 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 77 • A number will not be carried beyond a hyphen. For example, the number following 2-999 will be 2-000; 1-99-000 will follow 1-99-999. • The number following 9999999999999 will be 1. Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-39...
  • Page 78 Ð Edit Ð Ð Ð Store Ð Ð Ð : Entry : Run : Finish Normal : Interruption : Finish Abnormal ← → Return Figure 2-32: Analysis Process Screen (Closed Mode) 2-40 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 79 If automatic output has been set, the analysis results will be sent to the Graphic Printer, Data Printer, and/or Host Computer. If automatic output has not been set, the operator must select the data and destination for the output. See Chapter 4: Result Interpretation/Reports. Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-41...
  • Page 80 Press the Next No. keypad in the top line of the LCD. The Sample No. Setting screen will appear. Press the [Manual Mode] key to select manual analysis mode, and press the [ENTER] key. 2-42 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 81: Manual Cp Mode

    Use the type of blood collection tube shown below for Manual CP mode analyses. Diameter a: 12 - 15 mm Length b: 75 mm Overall length c: 82 mm or less Figure 2-34: Tube Dimensions Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-43...
  • Page 82 Sampler DP:1234567890123 XM HC GP DP CP Analysis Mode Closed Sample Number 1234567890123 Manual Mode Capillary Mode Closed Mode Ð ENTER CANCEL Figure 2-35: Sample No. Setting Screen (Manual CP/Closed Mode) 2-44 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 83 • A number will not be carried beyond a hyphen. For example, the number following 2-999 will be 2-000; 1-99-000 will follow 1-99-999. • The number following 9999999999999 will be 1. Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-45...
  • Page 84 Edit Ð Ð Ð Store Ð Ð Ð : Entry : Run : Finish Normal : Interruption : Finish Abnormal ← → Return Figure 2-37: Analysis Process Screen (Manual CP/Closed Mode) 2-46 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 85 If automatic output has been set up, the analysis results will be sent to the Graphic Printer, Data Printer, and/or Host Computer. If automatic output has not been set up, the operator must select the data and destination for output. See Chapter 4: Result Interpretation/Reports. Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-47...
  • Page 86 Press the Next No. keypad in the top line of the LCD. The Sample No. Setting screen will appear. Press the [Manual Mode] key to select manual analysis mode, and press the [ENTER] key. 2-48 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 87: Shutdown

    WARNING! CELLCLEAN (or 5% filtered sodium hypochlorite) is a strong alkaline solution. Avoid contact with it. If you get it on your skin or clothing, immediately wash it off with excess water. Sysmex SF-3000 Operator's Manual -- Revised October 1995 2-49...
  • Page 88 If you are going to shutdown the instrument, turn the Main Unit power OFF. Turn the printers off, if attached. NOTE: If you are going to continue to operate, perform a background check by pressing the [Restart] key displayed on the LCD. 2-50 Sysmex SF-3000 Operator's Manual -- Revised October 1995...

  • Page 89: Timer Mode

    Figure 2-42: Press the Start Switch CAUTION: If the Main Unit is not in Ready status, the Timer Mode does not function and the Pneumatic Unit power will not turn OFF. Sysmex SF-3000 Operator's Manual -- Revised October 1995 2-51...
  • Page 90 CHAPTER 3 WORK LIST INTRODUCTION ...............3-1 1.1 Order Inquiry Method.............3-1 EXECUTING A WORK LIST PROGRAM........3-2 2.1 Work List Display ............3-2 2.2 Data Entry ..............3-3 DOWNLOAD LIST..............3-6 3.1 From Host Computer (HC) ..........3-6 3.2 From Floppy Disk (FD) ..........3-8 DELETE................. 3-10 4.1 All Data ..............

  • Page 91: Chapter 3 Work List

    Perform inquiry of the Work List prior to analysis. Analyze the sample according to the order request, if registered. When the analysis order is not registered in Work List for that sample, the SF-3000 analyzes the sample. Analysis operation is performed by each order inquiry made by Sample ID No. or Rack-Tube Position No.

  • Page 92: Executing A Work List Program

    Cross (X) : Indicates that an analysis error occurred, preventing the completion of sample analysis. • [#/##] Number of marked samples/Number of registered samples in the list is indicated above [Repeat] keypad. Sysmex SF-3000 Operator's Manual --- Revised July 1996...

  • Page 93: Data Entry

    0 0 0 2 - 0 5 1 2 3 - 4 5 6 - 7 8 9 - 2 5 Down CANCEL Output Delete Load Figure 3-2: Rack - Tube Position No. Input Screen Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 94 Pressing this key places or removes a circle ( ) from the sample on which the cursor (displayed in reverse video) is placed. Place a circle to analyze the sample, or remove the circle for not to analyze the sample. Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 95 [QC] and [1] keys to specify the QC File No. 01. [ENTER] : Sets the input values. : Used as a backspace key to delete one character. Sysmex SF-3000 Operator's Manual -- Revised July 1996 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 96: Download List

    R a c k N o . ↓ R a c k N o . R a c k N o . Cancel ENTER Down CANCEL Output Delete Load Figure 3-5: HC Down Load Screen Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 97 N o . Download from HC R a c k N o . [ 0 / 1 0 ] Stop Cancel Down Output Delete Quit Load Figure 3-6: HC Down Loading Message Window Sysmex SF-3000 Operator's Manual -- Revised July 1996...

  • Page 98: From Floppy Disk (Fd)

    SAMPLE NO. ORDER Sample Rack Order Repeat Prev < Download from FD > Insert FD and press OK key. Cancel Mark Down Output Delete Return Load Figure 3-8: FD Down Load Screen Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 99 Error" will appear. Press [OK] key to close the window and retry the FD down load using a correct floppy disk, if needed. < FD Read Error > Press OK key. Figure 3-10: FD Read Error Window Sysmex SF-3000 Operator's Manual -- Revised July 1996 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 100: Delete

    Use this program to delete all the order information. Display the Work List on the screen. Press [Delete] key to display the Delete window. Press [All] key. The Delete Confirmation window will appear as shown in Figure 3-12 below. 3-10 Sysmex SF-3000 Operator's Manual -- Revised July 1996...

  • Page 101: Marked Data

    0 0 0 2 - 0 5 1 2 3 - 4 5 6 - 7 8 9 - 2 4 A l l Current Cancel Clear Figure 3-13: Mark Data Screen Sysmex SF-3000 Operator's Manual -- Revised July 1996 3-11...

  • Page 102: Current Data

    Press [Delete] key to display the Delete window. Press [Current] key. The Delete Confirmation window will appear. Press [OK] to delete the data with the cursor on it. Program deletes the data. 3-12 Sysmex SF-3000 Operator's Manual -- Revised July 1996...

  • Page 103: Gp Output

    Figure 3-15: GP Output Window Press [OK] key to print out the order information data to the Graphic Printer. Press [Cancel] key to stop the program and return to the Work List display. Sysmex SF-3000 Operator's Manual -- Revised July 1996 3-13...

  • Page 104: Backup

    1 2 3 - 4 5 6 Cancel 0 0 0 2 - 0 5 1 2 3 - 4 5 6 Down Output Delete Return Load Figure 3-16: Select Device Window 3-14 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 105 1 2 3 - 4 5 6 - 7 8 9 - 2 4 Down Output Delete Return Load Figure 3-17: FD Output Window Insert a floppy disk into the floppy disk drive. Figure 3-18: Insert Floppy Disk (Main Unit - Right Side) Sysmex SF-3000 Operator's Manual -- Revised July 1996 3-15...
  • Page 106 1 2 3 - 4 5 6 - 7 8 9 - 2 4 Down Output Delete Return Load Figure 3-19: FD Writing Window When writing the data is completed, the display returns to Work List display. 3-16 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 107 CHAPTER 4 RESULT INTERPRETATION/ REPORTS INTRODUCTION ...............4-1 INTERPRETIVE (IP) MESSAGES...........4-2 LIST DISPLAY/STORED DATA..........4-8 3.1 Sample Information Screen (Page 1)........4-10 3.2 CBC8 Screen (Page 2)..........4-13 3.3 WBC+5Diff# Screen (Page 3).........4-14 3.4 WBC+5Diff% Screen (Page 4)........4-15 3.5 RBC/PLT Screen (Page 5) ..........4-16 3.6 IP Messages Screen (Page 6) ..........4-18 GRAPHIC DISPLAY ............4-20 DATA VALIDATION ............4-24...
  • Page 108 11. OUTPUT STORED DATA ...........4-49 11.1 Current Data..............4-51 11.2 Marked Data..............4-52 11.3 All Data ..............4-52 11.4 Cancel..............4-53 11.5 Backup (FD) .............4-54 12. DELETE.................4-58 12.1 Current Data..............4-59 12.2 Marked Data..............4-60 12.3 All Data ..............4-61 13. AUTO OUTPUT...............4-62 Revised September 1995...

  • Page 109: Result Interpretation/Reports

    RESULT INTERPRETATION/REPORTS INTRODUCTION The SF-3000 is used to analyze and screen whole blood samples, and to store and reproduce the sample results. This chapter explains the screen displays, output and processing of analysis results in the Stored Data program. This chapter consists of the following major sections: INTERPRETIVE MESSAGES A significant feature of the SF-3000 is the use of Interpretive (IP) messages.

  • Page 110: Interpretive (Ip) Messages

    RESULT INTERPRETATION/REPORTS INTERPRETIVE (IP) MESSAGES The SF-3000 analyzes, displays and prints hematology information in a format designed to aid in the separation of POSITIVE or NEGATIVE data results. All analyzed samples without analysis errors can be separated into a POSITIVE and NEGATIVE category according to preset criteria.
  • Page 111 PLT Abn Scattergram (Morph) NRBC/PLT Clumps? Blasts/A-Lymph? Immature Gran? Aty/Abn Lympho? NOTE: When "WBC Abn Scattergram" is reported, only the affected parameters are dashed out. Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised February 1997...
  • Page 112 RESULT INTERPRETATION/REPORTS, IP Messages The IP messages used by the SF-3000 are listed on the following tables. NOTE: A [C] following the item number means that the message will appear with samples analyzed in the Capillary Mode. Item No. refers to the code displayed on the IP message screen in Stored Data (See Section 3.6 in this chapter).
  • Page 113 For Capillary mode analysis, the conditions applicable on IP messages given for normal analysis above also apply. In addition, IP messages only appear for the CBC-8 parameters, and only "POSITIVE" IP messages appear. Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 114 For Capillary mode analysis, the conditions applicable on IP messages given for normal analysis above also apply. In addition, IP messages only appear for the CBC-8 parameters, and only "POSITIVE" IP messages appear. Sysmex SF-3000 Operator's Manual -- Revised February 1997 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 115 For Capillary mode analysis, the conditions applicable on IP messages given for normal analysis above also apply. In addition, IP messages only appear for the CBC-8 parameters, and only "POSITIVE" IP messages appear. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 116: List Display/Stored Data

    RESULT INTERPRETATION/REPORTS LIST DISPLAY/STORED DATA The SF-3000 can store the analysis data, together with scattergrams, for up to 1000 samples in a hard disk. The stored analysis data is displayed in list form by using "List Display" of "Stored Data" program.
  • Page 117 [ → ], [ ← ] keys : Switch pages. [Prev] key : Cursor moves up one page (20 samples). [Next] key : Cursor moves down one page (20 samples). [Mark] key : Marks data. Refer to Section 6: Mark Data. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 118: Sample Information Screen

    : Indicates there is another sample with the same sample ID number on the same date. : Appears on the right of Sample No., indicates Sample No. was read by bar code reader. 4-10 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 119 : Indicates analysis error, excluding the ID read errors and the sample errors explained below. Result : Indicates analysis error caused by the sample, such as "SAMPLE NOT ASP ERROR", "LOW BLOOD VOLUME" or "LOW COUNT ERROR". Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-11...
  • Page 120 2 5 0 / 5 0 0 ( 8 0 0 ) Number of data results currently stored in memory Number of data results currently selected to be displayed Number of marked data results 4-12 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 121: Cbc8 Screen

    Indicates that the data is unreliable because of an abnormality detected in the sample. +, – (backlit in yellow): Indicates that the data is outside the Mark Limits. NOTE: The above indications are listed on the priority basis. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-13...

  • Page 122: Wbc+5Diff# Screen

    Indicates that the data is unreliable because of an abnormality detected in the sample. +, – (backlit in yellow): Indicates that the data is outside the Mark Limits. NOTE: The above indications are listed on the priority basis. 4-14 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 123 Indicates that the data is unreliable because of an abnormality detected in the sample. +, – (backlit in yellow): Indicates that the data is outside the Mark Limits. NOTE: The above indications are listed on the priority basis. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-15...

  • Page 124: Rbc/Plt Screen

    Indicates that the sample has abnormal particle size distribution or scattergram. W (backlit in yellow) : abnormal WBC scattergram R (backlit in yellow) : abnormal RBC particle size distribution P (backlit in yellow) : abnormal PLT particle size distribution 4-16 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 125 Indicates that the data is unreliable because of an abnormality detected in the sample. +, – (backlit in yellow): Indicates that the data is outside the Mark Limits. NOTE: The above indications are listed on the priority basis. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-17...

  • Page 126: Ip Messages Screen

    Indicates that this data is validated. Refer to Section 5: Data Validation in this chapter. 2) SAMPLE NO. Sample No., as read by bar code reader or input by the operator. 4-18 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 127 PLT Abn Distribution PLT Abn Dst ↓ Thrombo 2 (C] Thrombocytopenia ↑ 3 [C] Thrombocytosis Thrombo Item No. PLT Suspect Messages Abbreviation NRBC/PLT Clumps? NRBC/PLTC? Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-19 Sysmex SF-3000 Operator's Manual -- Revised February 1997...

  • Page 128: Graphic Display

    [g/dL] 32.9 RDW-SD [fL] PLT Flag 49.3 RDW-CV 14.7 Thrombo [x10 /uL] 250fL 40fL 11.9 [fL] 10.3 [fL] P-LCR 27.1 Edit List Output Delete Return Valid. lnfo. Figure 4-8: Graphic Screen 4-20 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 129 When pressing the [ERROR] key, the Analysis Error window as shown in Figure 4-9 will appear. << Analysis Error >> WBC/BASO Sampling Error Cancel Figure 4-9: Analysis Error Window Press the [Cancel] key to close the Analysis Error window. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-21...
  • Page 130 5 equal divisions. Refer to Chapter 10, Section 6: WBC Scattergrams. 8) RBC Particle Size Distribution Relative height curve showing RBC distribution. 9) PLT Particle Size Distribution Relative height curve showing PLT distribution. 4-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 131 If the total of abnormal plus suspect messages exceeds 5, suspect messages will get the priority, and a "t" mark in yellow back-light will appear on the right of the 5th message. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-23...

  • Page 132: Data Validation

    4.68 14.5 44.4 94.9 31.1 32.7 Mark Select More Return Graphic Search Sort Output Valid. Display Figure 4-10: Data Validation Screen NOTE: The '"v" mark is not displayed on Graphic Display. 4-24 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 133: Mark Data

    If there is any marked data that has not been output or deleted, the message window "## marked data has been exist!!!" appears when the [Mark] key is pressed. Delete the unprocessed mark(s), or delete or output the marked data. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-25...

  • Page 134: Select Display

    The List Display continues according to the selections made with this program until new settings are done. However, when the power is turned OFF, the list defaults to the original specifications of "All Samples". 4-26 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 135: Select By Flagging

    Morph. POS. : Data with an abnormality in blood cell morphology. Count POS. : Data with an abnormality in blood cell count. Error : Data with analysis errors (excluding ID READ errors). Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-27...
  • Page 136 More Return Graphic Search Sort Output Valid. Display Figure 4-14: Flagging Data List Window Pressing [Cancel] key stops the program without changing the listing and returns to the List Display screen. 4-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 137: Select By Date

    1995/12/30 1995/12/31 ♦ → Cancel ↑ ↓ Select Select Graphic Graphic Search Search Sort Sort Output Output More More Return Return Valid. Valid. Display Display Figure 4-15: Select by Date Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-29...
  • Page 138 Graphic Search Sort Valid. Display Figure 4-16: Select by Date List Window Press [OK] to set the date. The data for the specified date will be displayed on the List Display. 4-30 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 139: Select By No Output

    12/31 1 12/31 1 12/31 1 → 12/31 1 12/31 1 Cancel 12/31 1 Mark Select Graphic Search Sort Output Valid. More Return Display Figure 4-17: Select by No Output Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-31...
  • Page 140 Figure 4-18: Example of No Output Data Listing (Data that has not been output to DP) Pressing [Cancel] key stops the program without changing the listing and returns to the List Display screen. 4-32 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 141: Qc Data

    11:00 QC02* 1012 12/07 11:04 QC02* 1012 Next 12/07 11:05 1012 QC02* ← → Mark Select Edit Graphic More Search Sort Valid. Return Display lnfo. Figure 4-19: QC Data List Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-33...

  • Page 142: All Samples

    ERR0000000004 Func. 12/04 11:08 1012 12/04 11:08 ERR0000000005 1012 Func. ERR0000000006 Func. Mark 12/04 11:09 1012 Select More Return Graphic Search Sort Valid. Output Display Figure 4-20: All Samples List Screen 4-34 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 143: Read Fd

    1012 Func. 12/04 11:08 ERR0000000004 1012 Func. 12/04 11:08 ERR0000000005 1012 Func. 12/04 11:09 ERR0000000006 Mark Func. Select Graphic More Search Sort Return Output Valid. Display Figure 4-21: FD Read Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-35...
  • Page 144 Func. 12/04 11:08 ERR0000000004 1012 Func. 12/04 11:08 ERR0000000005 1012 Func. 12/04 11:09 ERR0000000006 Mark Func. Select Graphic More Search Sort Return Output Valid. Display Figure 4-23: FD Reading Message Window 4-36 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 145 Do not remove the floppy disk before you have exited the FD Read program. If the floppy disk was removed before exiting FD Read program, the list will be automatically changed to List Display of All Samples. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-37...

  • Page 146: Search Stored Data

    Display. Press [Bottom] key to display the end of the list at the bottom of the List Display. Pressing [Cancel] key cancels the program and returns to the List Display screen. 4-38 Sysmex SF-3000 Operator's Manual -- Revised October 1995...

  • Page 147: By Sample No

    Display Display Figure 4-27: Sample No. Input Screen Data with the specified sample No. will be searched, and the specified data will be displayed at the top of the List Display. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-39...

  • Page 148: Same Day

    Press [Bottom Same Day] key. The analysis data which was found at the last for the specified date will be displayed on the list. Pressing [Cancel] key cancels the program and returns to the List Display screen. 4-40 Sysmex SF-3000 Operator's Manual -- Revised September1995...

  • Page 149: By Date

    © 1995/12/31 Å® → ↑ ↓ Cancel Select Select Graphic Graphic Search Search Sort Sort Output Output More More Return Return Valid. Valid. Display Display Figure 4-29: Search by Date Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-41...
  • Page 150 Output More Return Graphic Search Sort Valid. Display Figure 4-30: Search by Date List Window Pressing [Cancel] key stops the program without changing the listing and returns to the List Display screen. 4-42 Sysmex SF-3000 Operator's Manual -- Revised September1995...

  • Page 151: Sort Stored Data

    1012 CANCEL 12/04 11:08 ERR0000000004 1012 Func. ERR0000000005 Func. 12/04 11:08 1012 Mark 12/04 11:09 ERR0000000006 1012 Func. Select Graphic Search Sort Output More Return Valid. Display Figure 4-31: Sort Screen Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-43...
  • Page 152 12/04 11:08 ERR0000000004 1012 Func. ERR0000000005 1012 Func. 12/04 11:08 Mark 12/04 11:09 ERR0000000006 1012 Func. Select Output More Return Graphic Search Sort Valid. Display Figure 4-32: Sequence Order List Screen 4-44 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 153: Sample No. Order

    – < ... < 0 < ... < 9 < ... < A < ... < Z NOTE: After the instrument is powered OFF, the List Display will be automatically arranged in chronological order. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-45...

  • Page 154: Edit Sample Information

    1012 Func. Edit More Delete Return Info. Figure 4-34: Edit Sample Info. Window Press the appropriate keypad to edit the desired sample information. Refer to the following sections for the procedures. 4-46 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 155: Sample No

    • When the list is sorted in Sample ID No. order, the sample will automatically be moved according to its new sample No. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-47...

  • Page 156: Id Read Status

    POS column in List Display (P1). The flag item numbers in List Display (P6) disappear. On the graphic display, "Modified" is displayed under the NEGATIVE status indicator of the sample. 4-48 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 157: Output Stored Data

    When the [Output] key is pressed while a graphic display appears on the LCD, Select Device window as shown in Figure 4-38 will appear. Only the displayed sample will be output. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-49...
  • Page 158 GP List : The specified data is printed in a line print format. : The specified data is transmitted to the host computer. Refer to the following sections for the procedures. 4-50 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 159: Current Data

    To cancel the data output after it has begun, press [Output] and then [Cancel Output] key in the Select Sample window. Refer to Section 11.4: Cancel (Output). NOTE: Wait until printing is complete before specifying another output for printing. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-51...

  • Page 160: Marked Data

    To cancel the data output after it has begun, press [Output] and then [Cancel Output] key in the Select Sample window. Refer to Section 11.4: Cancel (Output). NOTE: Wait until printing is complete before specifying another output for printing. 4-52 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 161: Cancel

    Select a device by pressing [DP], [GP Graph], [GP List] or [HC] keypad. The output to the selected device will be canceled. Press [Cancel] to quit the cancellation and to return to the List Display. The output being executed is continued. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-53...

  • Page 162: Backup (Fd)

    Cancel 12/04 11:08 ERR0000000004 1012 Func. ERR0000000005 Func. 12/04 11:08 1012 Mark 12/04 11:09 ERR0000000006 1012 Func. Select Graphic Search Sort Output More Return Valid. Display Figure 4-40: Select Sample Window 4-54 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 163 Func. 12/04 11:08 ERR0000000004 1012 Func. 12/04 11:08 ERR0000000005 1012 Func. 12/04 11:09 ERR0000000006 Mark Func. Select Graphic Search Sort More Return Valid. Output Display Figure 4-42: Backup Confirmation Message Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-55...
  • Page 164 12/04 11:08 ERR0000000004 1012 Func. 12/04 11:08 ERR0000000005 1012 Func. Mark 12/04 11:09 ERR0000000006 Func. Select Graphic More Search Sort Return Output Valid. Display Figure 4-44: FD Write Error Message Window 4-56 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 165 Insert the correct type of floppy disk into the drive, and press [OK] key. Pressing [Cancel] cancels writing process and exits the program. When the data has been written onto the floppy disk, the program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-57...

  • Page 166: Delete

    Info. Figure 4-45: Select Sample Window Press the desired keypad to specify the data to be deleted. Press [OK] to delete the data. Refer to the following sections for the procedure. 4-58 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 167: Current Data

    Press [OK] to delete the data with the cursor on it. Program deletes the data and exits. The screen returns to List Display. Press [Cancel] to end the program without deleting the data. NOTE: The data cannot be restored once deleted. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-59...

  • Page 168: Marked Data

    Press [OK] to delete the marked data. Program deletes the data and exits. The screen returns to List Display. Press [Cancel] to end the program without deleting the data. NOTE: The data cannot be restored once deleted. 4-60 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 169: All Data

    Figure 4-48: Delete Confirmation Window Press [OK] to delete all stored sample data. Program ends. Press [Cancel] to end the program without deleting the data. NOTE: The data cannot be restored once deleted. Sysmex SF-3000 Operator's Manual -- Revised September 1995 4-61...

  • Page 170: Auto Output

    RESULT INTERPRETATION/REPORTS 13. AUTO OUTPUT The SF-3000 automatically prints out analysis data to a Data Printer and Graphic Printer, and automatically transmits analysis data to the Host Computer. Data is output according to preset conditions. The Auto Output function outputs only data that is validated for reporting and has not previously been output.
  • Page 171 CHAPTER 5 MAINTENANCE & SUPPLIES INTRODUCTION .............. 5-1 SF-3000 MAINTENANCE CHECK LIST........5-3 DAILY MAINTENANCE............5-4 Clean Detector Chamber and Manometer (Shutdown)....5-4 Remove Fluid from Pneumatic Unit Trap Chamber ....5-6 WEEKLY MAINTENANCE ..........5-7 Clean SRV Tray............5-7 Execute Clean WBC Detector Sequence.......

  • Page 172: Chapter 5 Maintenance & Supplies

    MAINTENANCE & SUPPLIES INTRODUCTION To keep the SF-3000 in optimal operating condition, periodic maintenance is required. Use the schedule below as a guide, and record your work on the maintenance check list found in Section 2 of this chapter. • DAILY: Clean detector chambers and manometers (shutdown sequence).
  • Page 173 MAINTENANCE & SUPPLIES WARNING! When working with the front cover open, make sure that the stop bar is in place. Stop Bar Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 174 Rubber Plate No. 39 Clean RBC Aperture Anti-Shock Rubber Remove WBC Flow Cell Air Bubble No. 49 Sheath Flow Reagent Replace Sequence Fuse Replace Waste Container (if used) Motor Belt No. 6 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 175: Daily Maintenance

    WARNING! CELLCLEAN (or 5% filtered sodium hypochlorite) is a strong alkaline solution. Avoid contact with it. If you get it on your skin or clothing, immediately wash it off with excess water. Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised October 1995...
  • Page 176 If you are going to shutdown the instrument, turn the Main Unit power OFF. Turn the printers OFF, if attached. NOTE: If you are going to continue to operate, perform a background check by pressing the [Restart] key displayed on the LCD. Sysmex SF-3000 Operator's Manual -- Revised October 1995...

  • Page 177: Remove Fluid From Pneumatic Unit Trap Chamber

    Figure 5-3-4: Trap Chamber (Front of the Pneumatic Unit) Discard the fluid; then reattach the chamber. CAUTION: If fluid collects every day, contact a Sysmex service representative. Keep your log on the maintenance check list. Sysmex SF-3000 Operator's Manual -- Revised October 1995...

  • Page 178: Weekly Maintenance

    Manual Aspiration Pipette on the unit. Rinse the tray with water and wipe dry. See Figure 5-4-2. Tray SRV Tray Thumbscrew Figure 5-4-1: Remove SRV Tray Figure 5-4-2: Rinse SRV Tray (View from the instrument left side) Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 179 • Replace the tray in the correct position. • Make sure that the thumbscrew on the Manual Aspiration Pipette is tightened. Release the stop bar; then close the front cover. Record the cleaning on the maintenance check list. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 180: Execute Clean Wbc Detector Sequence

    DP:1234567890123 XM HC GP DP SU Maintenance Clog Drain Service Service More Reagent Test Return Removal Sample Sequence Data Figure 5-4-3: Maintenance Second Menu Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 181 XM HC GP DP SU Maintenance < Service Sequence > <Clean WBC Detector> It will take approx. 5 min. Press Start Switch. Cancel Return Figure 5-4-5: Clean WBC Detector Execute Message 5-10 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 182 Place 1 mL each of CELLCLEAN both in the Reaction Chambers 1 and 2. Press Start Switch. Return Figure 5-4-7: Cleaning WBC Detector Message (2) Sysmex SF-3000 Operator's Manual -- Revised March 1996 5-11 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 183 • If reagent spills on painted surfaces of the instrument, immediately wipe it up with a damp tissue. Reaction Chamber 2 Reaction Chamber 1 Figure 5-4-8: Place CELLCLEAN 5-12 Sysmex SF-3000 Operator's Manual -- Revised March 1996 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 184 NOTE: Make sure to reset the counter. If not, the message "Clean the WBC Detector" will remain on the LCD. (12) Record the cleaning on the maintenance check list. Sysmex SF-3000 Operator's Manual -- Revised March 1996 5-13...

  • Page 185: Monthly Maintenance

    The cleaning tool will only fit into the orifice approximately 1.0 cm. Orifice Loosen Orifice Cleaning Tool Figure 5-5-1: Remove Orifice Figure 5-5-2: Clean Orifice (Main Unit Left Side View) 5-14 Sysmex SF-3000 Operator's Manual -- Revised July 1996 Sysmex SF-3000 Operator's Manual -- Revised March 2001...
  • Page 186 NOTE: Make sure to reset the counter after cleaning. If not, the message "Clean the Orifice" will remain on the LCD. Record the cleaning on the maintenance check list. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-15...

  • Page 187: Execute Clean Waste Chamber Sequence

    Manual Mode Next No. 1234567890123 Sysmex Sampler DP:1234567890123 XM HC GP DP SU Maintenance Clog Drain Service Service More Reagent Test Return Removal Sample Sequence Data Figure 5-5-4: Maintenance Second Menu 5-16 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 188 XM HC GP DP SU Maintenance <Service Sequence> < Clean Waste Chamber > It will take approx. 20 min. Press Start Switch. Cancel Return Figure 5-5-6: Clean Waste Chamber Execute Message Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-17...
  • Page 189 Maintenance <Service Sequence> <Clean Waste Chamber> Place 1 mL each of CELLCLEAN both in the Reaction Chambers 1 and 2. Press Start Switch. Return Figure 5-5-8: Cleaning Waste Chamber Message (2) 5-18 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 190 • If reagent spills on painted surfaces of the instrument, immediately wipe it up with a damp tissue. Reaction Chamber 2 Reaction Chamber 1 Figure 5-5-9: Place CELLCLEAN Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-19 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 191 NOTE: Make sure to reset the counter. If not reset, the message "Clean the Waste Chamber" will remain on the LCD. (12) Record the cleaning on the maintenance check list. 5-20 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 192: Clean Sample Rotor Valve (Srv)

    When removing the SRV tray, do not loosen the thumbscrew fixing the Manual Aspiration Pipette on the unit. SRV Tray Thumbscrew Figure 5-5-11: Remove SRV Tray (View from the instrument left side) Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-21 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 193 • Take care not to bend the Manual Aspiration Pipette when pulling out the valve. Pull the valve sections gently toward you. See Figure 5-5-14. SRV Set Figure 5-5-14: Remove SRV Set (View from the Instrument Left Side) 5-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 194 CELLCLEAN; however, completely wipe off the CELLCLEAN to prevent problems in other parts of the instrument. • Do not damage the sampling valve surface in any way; this can cause leaks. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-23...
  • Page 195 (13) Flush the SRV tray with tap water, and replace it. (14) Slowly and gently push up the Manual Rinse Mechanism. (15) Release the stop bar and close the front cover. 5-24 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 196 (18) Reset the SRV counter by referring to Section 9: Reset the Counter in this chapter. NOTE: Make sure to reset the counter. If not reset, the message "Clean the SRV" will remain on the LCD. (19) Record the cleaning on the maintenance check list. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-25...

  • Page 197: Yearly Maintenance

    DIRECT EYE EXPOSURE" are visible as shown in Figure 5-6-1. Semiconductor Laser Unit Cover < Left Side View > Figure 5-6-1: Location of Laser Unit and Labels (Left Interior of the Main Unit) 5-26 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 198 If the optical system cover has detectable opening, defective or damaged parts, contact Sysmex service representative to repair, replace or adjust it before turning on the power to the instrument.
  • Page 199 If any light leakage is evident, IMMEDIATELY TURN OFF THE POWER TO THE MAIN UNIT and disconnect the power cord from the AC outlet. Contact Sysmex service representative for repair. Record the inspection on the maintenance check list. 5-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 200: As Needed Maintenance

    Figure 5-7-1: Pull Down the Rinse Cup Pull the rinse cup forward to you (arrow ) and disconnect the two tubes (arrows ). See Figure 5-7-2. Manual Rinse Figure 5-7-2: Disconnect the Tubes Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-29...
  • Page 201 • To prevent a "Rinse Motor Function Error", make sure that the rinse cup mechanism is at its uppermost position. Turn ON the power. (10) Record the cleaning on the maintenance check list. 5-30 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 202: Execute Rbc Aperture Clog Removal Sequence

    XM HC GP DP SU Maintenance Clog Drain Service Service More Reagent Test Return Removal Sample Sequence Data Figure 5-7-5: Maintenance Second Menu Press the [Clog Removal] key to execute the clog removal sequence. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-31...
  • Page 203 After the Clog Removal sequence is completed, the instrument returns to the ready status. Record the clog removal on the maintenance check list. NOTE: If the error persists, execute the clean transducer sequence by referring to the following Section 7.3. 5-32 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 204: Execute Clean Rbc Transducer Sequence

    XM HC GP DP SU Maintenance <Service Sequence> Clean WBC Flow Cell Waste Chamber Air Bubble Removal Clean Replace Sheath WBC Detector Flow Reagent Clean RBC Transducer Return Figure 5-7-7: Service Sequence Submenu Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-33...
  • Page 205 Next No. 1234567890123 Running Sampler Sysmex DP:1234567890123 XM HC GP DP SU Maintenance Draining reagent in the RBC Transducer. Please wait. Clean RBC Transducer Return Figure 5-7-9: Cleaning Transducer Message Window (1) 5-34 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 206 WARNING! CELLCLEAN (or 5% filtered sodium hypochlorite) is a strong alkaline solution. Avoid contact with it. If you get it on your skin or clothing, immediately wash it off with excess water. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-35 Sysmex SF-3000 Operator's Manual -- Revised February 1996...
  • Page 207 XM HC GP DP SU Maintenance <Service Sequence> Cleaning RBC Transducer. 0 min. / 2 min. Clean RBC Transducer Return Figure 5-7-13: Cleaning Transducer Message Window (3) 5-36 Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 208 (11) After the cleaning sequence is completed, the instrument automatically performs auto rinse and returns to the Ready status. (12) Release the stop bar and close the front cover. (13) Record the cleaning on the maintenance check list. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-37...

  • Page 209: Clean Rbc Detector Aperture

    Please wait. Figure 5-7-14: Press [Drain Sample] Turn OFF the power to the Main Unit. Wait at least 30 seconds to release the pressure and vacuum accumulated in the pneumatic lines. 5-38 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 210 Figure 5-7-16: RBC Detector Prepare a gauze or lint-free tissue to absorb any reagent from the transducer chamber. Remove the plug to access the transducer aperture (Figure 5-7-17). Plug Figure 5-7-17: Remove the Plug Sysmex SF-3000 Operator's Manual -- Revised February 1996 5-39...
  • Page 211 DIFF Channel [x 10 /µL] 0.02 [x 10 /µL] [g/dL] [x 10 /µL] (11) Keep the cleaning record on the maintenance check list. 5-40 Sysmex SF-3000 Operator's Manual -- Revised February 1996 Sysmex SF-3000 Operator's Manual -- Revised March 2001...

  • Page 212: Execute Wbc Flow Cell Air Bubbles Removal Sequence

    Manual Mode Next No. 1234567890123 Sysmex Sampler DP:1234567890123 XM HC GP DP SU Maintenance Clog Drain Service Service More Reagent Test Return Removal Sample Sequence Data Figure 5-7-19: Maintenance Second Menu Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-41...
  • Page 213 RBC Transducer Return Figure 5-7-21: Removing WBC Flow Cell Air Bubbles After the rinse sequence is completed, the instrument returns to the Ready status. Keep the record on the maintenance check list. 5-42 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 214: Execute Replace Sheath Flow Reagent Sequence

    Manual Mode Next No. 1234567890123 Sysmex Sampler DP:1234567890123 XM HC GP DP SU Maintenance Clog Drain Service Service More Reagent Test Return Removal Sample Sequence Data Figure 5-7-22: Maintenance Second Menu Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-43...
  • Page 215 RBC Transducer Return Figure 5-7-24: Replacing Sheath Flow Reagent Message After the replacing sequence is completed, the instrument returns to the Ready status. Keep the record on the maintenance check list. 5-44 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 216: Replace Waste Container (If Used)

    Use the cap removed in step (2) above to cap the full container. Dispose the waste in accordance with your laboratory policy and existing hospital, laboratory, and government regulations. Record the replacement on the maintenance check list. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-45...

  • Page 217: Replacements

    Loosen the cap of the empty reagent container, and pull the Cubitainer spout kit tubing straight out. Insert the spout kit tubing straight into the new reagent container, and tighten the cap. 5-46 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 218 Move the cursor to the reagent that was replaced using the [↑] and [↓] keys. Use the [←] and [→] keys to move the cursor to the lot number or expiration date column. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-47...
  • Page 219 B 2 0 0 3 1 0 / 3 1 / 9 5 D 3 0 3 9 1 1 / 0 6 / 9 5 ENTER CANCEL Return Figure 5-8-3: Expiration Date Input Screen 5-48 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 220 0.02 [x 10 /µL] [g/dL] [x 10 /µL] (16) Open the reagent replacement log by referring to Section 8.1.1: Reagent Log on the next page to verify the log is updated. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-49...

  • Page 221: Reagent Log

    3 / 9 6 Page Page Return Down Figure 5-8-4: Reagent Log Screen Use the [Page Up] and [Page Down] keys to change the reagent log pages. Press [Return] to exit the program. 5-50 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 222: Replace The Piercer (Auto Sampler Unit)

    Loosen the two piercing unit thumbscrews shown in Figure 5-8-6 so that the piercing unit is mobile. Loosen Two Thumbscrews Loosen CP Cover Loosen Figure 5-8-5: Remove CP Cover Figure 5-8-6: Loosen the Thumbscrews Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-51...
  • Page 223 Figures below), then lower the unit to lay down (Arrow Piercing Unit Figure 5-8-9: Pull the Piercing Unit Figure 5-8-10: Pull the Piercing Unit Figure 5-8-11: Lower the Piercing Unit (Cross Section View) (Cross Section View) 5-52 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 224 Figure 5-8-14: Unit has been locked (Cross Section View) (Cross Section View) Disconnect the three tubes shown Disconnect in Figure 5-8-15. CAUTION: Do not cut or bend the tubes. Disconnect Figure 5-8-15: Disconnect the Tubes Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-53...
  • Page 225 You must first loosen the fixing screw before removal. (10) Loosen the two thumbscrews shown in Figure 5-8-17, and remove the piercer. Loosen Two Thumbscrews Figure 5-8-17: Loosen Two Thumbscrews 5-54 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 226 WARNING! After tightening the needle fixing screw, the piercer needle may be exposed. Piercer Needle Reconnect the three tubes as shown in Figure 5-8-20. Connect Connect Figure 5-8-20: Reconnect the Tubes Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-55...
  • Page 227 Tighten the two piercing unit thumbscrews to secure the piercing unit (see Figure 5-8-23). Mixer Mechanism Tighten Two Thumbscrews Holding Metal Tighten Tighten Up-Down Air Cylinder Figure 5-8-22: Remove the Holding Metal Figure 5-8-23: Tighten the Thumbscrews 5-56 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 228 Reset the piercer counter by referring to Section 9 in this chapter. NOTE: Make sure to reset the counter. If not reset, the message "Replace Piercer" will remain on the LCD. (10) Record the replacement on the maintenance log. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-57...

  • Page 229: Replace The Piercer (Manual Cp Unit)

    Pull the Manual CP Unit cover straight up to remove it. Refer to Figure 5-8-24. Open the tube lid (see Figure 5-8-25). Tube Lid Unit Cover Tube Holder Figure 5-8-24: Remove the Unit Cover Figure 5-8-25: Open the Tube Lid 5-58 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 230 Loosen the two thumbscrews shown in Figure 5-8-28, and remove the piercer. Figure 5-8-27: Lock the Piercer Figure 5-8-28: Loosen the Thumbscrews Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-59...
  • Page 231 ) as shown in Figure 5-8-31. Reset the Manual CP cover. CAUTION: Take care not to pinch or bend the tubing when replacing the cover. Figure 5-8-31: Reconnect the Tubes Figure 5-8-32: Set the Unit Cover 5-60 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 232 Reset the piercer counter by referring to Section 9 in this chapter. NOTE: Make sure to reset the counter. If not reset, the message "Replace Piercer" will remain on the LCD. Record the replacement on the maintenance check list. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-61...

  • Page 233: Replace The Hand Clipper (Auto Sampler Unit)

    Reset the CP cover and turn ON the power. CAUTION: Take care not to pinch or bend the tubing when replacing the cover. Record the replacement on the maintenance log. 5-62 Sysmex SF-3000 Operator's Manual -- Revised December 1995 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 234: Replace The Rubber Plate No. 39 (Auto Sampler Unit)

    Reset the CP cover and turn ON the power. CAUTION: Take care not to pinch or bend the tubing when replacing the cover. Record the replacement on the maintenance log. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-63 Sysmex SF-3000 Operator's Manual -- Revised March 2001...
  • Page 235 Reset the CP cover and turn ON the power. CAUTION: Take care not to pinch or bend the tubing when replacing the cover. Record the replacement on the maintenance check list. 5-64 Sysmex SF-3000 Operator's Manual -- Revised December 1995 Sysmex SF-3000 Operator's Manual -- Revised March 2001...

  • Page 236: Replace The Fuses

    Table 5-8-4: Pneumatic Unit Fuses Specification Part No. Description Fuse Type Location 117 VAC 266-5011-3 Fuse 250V4A ST4-4A-N1 Time Lag Right Side 220/240 VAC 266-5292-6 Fuse 250V2A No.19195 Time Lag Right Side Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-65...
  • Page 237 MAINTENANCE & SUPPLIES, Replacements Connect the power cord and turn ON the power. Record the replacement on the maintenance log. 5-66 Sysmex SF-3000 Operator's Manual -- Revised December 1995 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 238: Replace The Belt No. 6

    Disconnect the connector to the Mixing Motor shown in figure below. Mixing Motor Pulley Disconnect this connector. Pulley Figure 5-8-40: Mixing Motor Remove the Belt No. 6. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-67...
  • Page 239 1600 - 2000 rpm. NOTE: Refer to Chapter 8, Section 3.3: Test Motor for the test program. (12) Record the replacement on the maintenance log. 5-68 Sysmex SF-3000 Operator's Manual -- Revised December 1995 Sysmex SF-3000 Operator's Manual -- Revised March 2001...

  • Page 240: Reset The Counter

    Manual Mode Next No. 1234567890123 Sysmex Sampler DP:1234567890123 XM HC GP DP SU Maintenance Clog Drain Service Service More Reagent Test Return Removal Sample Sequence Data Figure 5-9-1: Maintenance Second Menu Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-69...
  • Page 241 1 1 2 1 3 Vacuum Orifice 1 2 5 0 Reset Waste Chamber 1 2 5 0 Reset WBC Detector 4 3 0 Reset ← → Return Figure 5-9-3: Counters Screen 5-70 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 242 Figure 5-9-5: Reset Counters Confirmation Screen Press [Continue] to return to the Counter screen. Press [Set] to reset the counter. Press [Cancel] to cancel the counter reset and exit the Service Data program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-71...

  • Page 243: Format Fd

    Next No. 1234567890123 Ready Sampler Sysmex DP:1234567890123 XM HC GP DP SU Maintenance Insert disk into FD drive and press OK key. Cancel Format Version More Return Check Figure 5-10-2: Format FD Screen 5-72 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 244 Press [Yes] to format another disk, or press [No] to end the program. When the disk formatting fails, "Cannot format this disk. Format another?" message appears. Press [Yes] to retry formatting, or press [No] to end the program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 5-73...
  • Page 245 Fuse 250V3.15A No.19195 Chapter 5, Section 8.7 266-5011-3 Fuse 250V4A ST4-4A-N1 Chapter 5, Section 8.7 266-5292-6 Fuse 250V2A No.19195 Chapter 5, Section 8.7 342-2626-4 Belt No. 6 Chapter 5, Section 8.8 5-74 Sysmex SF-3000 Operator's Manual -- Revised March 1998...
  • Page 246 CHAPTER 6 QUALITY CONTROL INTRODUCTION ............6-1 1.1 Types of QC Method........... 6-2 1.2 QC Parameters To Be Monitored ........6-2 DELETE FILE..............6-3 SYSTEM SETTINGS ............6-5 QC PARAMETER SELECTION..........6-7 4.1 Selecting QC Parameter ( X /L-J Control) ......6-7 4.2 Selecting QC Parameter ( X M Control)......

  • Page 247: Chapter 6 Quality Control

    -- whether the same values are obtained when analysis is repeated on a sample). Two types of QC are used in the SF-3000: Individual QC, which is performed on individual samples, and Statistical QC, which uses statistical methods. • Individual QC : Refers to the function that monitors samples individually.

  • Page 248: Types Of Qc Method

    Control, the operator should check the X M control chart, and execute X Control or L-J Control to verify the control data results of X or L-J Control. In addition to the 23 QC parameters normally displayed on the screen, the SF-3000 also has 5 other sensitivity-related parameters for a total of 28 parameters.

  • Page 249: Delete File

    1 9 9 6 / 0 5 / 3 1 High Manual ↓ Cancel Plot Plot File File Return Return Figure 6-1: Delete File Selection Screen Using the [↑] and [↓] keys, select the file to be deleted. Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 250 File No. 1 will be deleted. Cancel Figure 6-2: Delete Confirmation Message Press [OK] to delete the specified file and end the program. Press [Cancel] to cancel the deletion and end the program. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 251: System Settings

    2 8 . 5 25.8 31.8 MCHC 28.9 2 9 . 3 26.0 System X / L - J Return Settings Param. Param. Figure 6-3: QC Settings Submenu Select [System Settings] from the submenu. Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 252 Changing from X Control to L-J Control or vice-versa on a daily basis should not be done. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 253: Qc Parameter Selection

    Use the [←] and [→] keys to specify "Used" (if the parameter is to be included in QC analysis) or "Not Used" (if the parameter is to be excluded from QC analysis). The selected setting is displayed in reverse video. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 254: Selecting Qc Parameter ( X M Control)

    Not Used MCHC Used Not Used ← RDW-SD Used Not Used RDW-CV Used Not Used Used Not Used → Used Not Used Used Not Used Cancel Figure 6-6: X M Parameters Setting Window Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 255 They are just excluded from being plotted on the QC chart. If the operator changes "Not Used" parameter to a "Used" parameter, all previously excluded control data will be included on the QC chart. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 256: Setting X M Control

    Input the password and press [ENTER]. The X M setting window will appear, and the current number of samples per batch will be displayed as below. XM Control Batch Cancel Start Figure 6-7: X M Control Settings Window 6-10 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 257 To stop the X M Control, press [ X M Start/Stop] keypad. The X M Control window as shown below appears. XM Control Cont. Stop Figure 6-9: X M Control Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-11...
  • Page 258 X M Control will judge as an abnormality to be present. In this case, set X M to "Stop", so that these samples will not be included in X M computation. 6-12 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 259: Target/Limit

    (The cursor will appear at the top of the screen.) However, if the cursor is on X M, no downward scrolling will occur. Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-13...
  • Page 260 RBC, HGB, HCT, MCV, MCH, MCHC P2. PLT+5Calc. RDW-SD, RDW-CV, PLT, PDW, MPV, P-LCR P3. WBC+5DIFF# WBC, NEUT#, LYMPH#, MONO#, EO#, BASO# P4. DIFF Sensitivity GRAN-X, GRAN-Y, WBC/BA-X, WBC/BA-Y, GRAN-Y (W) 6-14 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 261: Read Fd (Floppy Disk)

    QUALITY CONTROL, Target/Limit 6 . 2 Read FD (Floppy Disk) Floppy disk provided with the Sysmex control blood includes the control blood information and target and limit# (%) values for both the manual and closed modes. This information can be registered automatically by using the "Read FD" program.
  • Page 262 Pressing the [↑] when the cursor has reached the top of the list will move the cursor to the bottom of the list. 6-16 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 263 (10) Press [OK]. The selected FD file assay value for the selected item is read into the file specified and the program exits. Pressing [Cancel] exits the program without reading the data from floppy disk. Sysmex SF-3000 Operator's Manual -- Revised October 1995 6-17...
  • Page 264 QUALITY CONTROL, Target/Limit, Read FD This page is intentionally left blank. 6-18 Sysmex SF-3000 Operator's Manual -- Revised October 1995...

  • Page 265: Help

    L I M I T # Read Help Setting Return Into. List Figure 6-14: Help Insert FD Message Window Insert the QC disk into floppy disk drive, and press [OK] key. Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-19...
  • Page 266 SF CHECK; Hematology control blood [INTENDED USE] SF CHECK is a hematology reference control for evaluating the accuracy and precision of the SF-3000 instrument system that measures the hematology values. Results must be obtained through analysis in the instrument Quality Control Analysis Mode.

  • Page 267: Auto Setting

    Use the [↑] and [↓] keys to move the cursor. Select the specific file in which target and limit values are to be registered. Press [Settings] keypad. The password entry screen will appear. Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-21...
  • Page 268 5.24 0.26 5.24 LYMPH# 1.41 0.14 1.41 MONO# 1.00 0.30 1.00 Quit 2.46 0.25 2.46 BASO# 6.39 0.41 6.39 Read Help Setting Return Into. List Figure 6-18: Auto Target Setting Screen 6-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 269 1.41 0.14 1.41 0.28 MONO# 1.00 0.30 1.00 0.60 Quit 2.46 0.25 2.46 0.50 BASO# 6.39 0.41 6.39 0.83 Read Help Setting Return Into. List Figure 6-20: Auto Limit Setting Screen Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-23...
  • Page 270 Figure 6-21: Confirmation Message Window (10) Press [OK] key to set the new settings into the specified file and end the program. Press [Cancel] to cancel the new settings and end the program. 6-24 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 271: Manual Change

    Use the [↑] and [↓] keys to move the cursor. Select the specific file in which target and limit values are to be registered. Press [Settings] keypad. The password entry window will appear. Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-25...
  • Page 272 LYMPH# 1.41 0.14 1.41 0.28 MONO# 1.00 0.30 1.00 0.60 2.46 0.25 2.46 0.50 BASO# 6.39 0.41 6.39 0.83 QUIT Read Help Setting Return Into. List Figure 6-24: Manual Setting Window 6-26 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 273 Figure 6-25: Confirmation Message Window (12) Press [OK] key to set the new settings into the specified file and end the program. Press [Cancel] to cancel the new settings and end the program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-27...

  • Page 274: Lot Information

    Use the [↑] and [↓] keys to move the cursor. Select the specific file in which control data information is to be registered. Press [Lot Info.] keypad from the submenu. The password entry window will appear. 6-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 275 Mode : Pressing [Change] key alters the analysis mode "Manual" and "Closed". NOTE: When the cursor moves to "Level" or "Mode", the numeric keypads disappears and the [Change] keypad appears instead. Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-29...
  • Page 276 Figure 6-28: Lot Information Confirmation Window Press [OK] to write the input values into the specified file, and end the program. Press [Cancel] to cancel the new settings and end the program. 6-30 Sysmex SF-3000 Operator's Manual -- Revised October 1995...

  • Page 277: Qc Chart

    P2. PLT+5Calc. RDW-SD, RDW-CV, PLT, PDW, MPV, P-LCR P3. WBC+5DIFF# WBC, NEUT#, LYMPH#, MONO#, EO#, BASO# P4. 5DIFF% NEUT%, LYMPH%, MONO%, EO%, BASO% P5. DIFF Sensitivity GRAN-X, GRAN-Y, WBC/BA-X, WBC/BA-Y, GRAN-Y (W) Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-31...
  • Page 278 X M Control : If data exceeds the preset control limit in the same direction twice consecutively. However, if calibration has been performed, apply this criterion only to data since the last calibration. 6-32 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 279: Select File

    Use the [↑] and [↓] keys to move the cursor, and press [OK]. The QC Chart for the specified file No. will appear. Pressing [Cancel] key will cancel the file selection and end the program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-33...

  • Page 280: Output Qc Chart/Data

    Using the [←] and [→] keys, move the cursor to specify the range of data to be output. NOTE: The data on which the cursors are positioned is included in the range specified. 6-34 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 281 : Press to print the QC data of specified range to a Graphic Printer. [HC] : Press to output the QC data of specified range to the Host Computer. Device Chart List Cancel Figure 6-32: Output Device Selection Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-35...

  • Page 282: Mean/Sd (Calculation)

    If there is unsuitable data for target and limit value computations, sandwich the data between the 2 cursors by using the [←] and [→] keys. Then, press [Exclude] keypad. The exclusion confirmation message will appear. 6-36 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 283 Refer to Section 6.4: Auto Setting in this chapter. Calculated data will be set into reference data. O K ? Cancel Figure 6-35: Set Reference Confirmation Window Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-37...

  • Page 284: Delete Plot

    Use the [↑] and [↓] keys to switch screens. Using the [←] and [→] keys, move the cursor to specify the range of data to be deleted. NOTE: The data on which the cursors are positioned are also deleted. 6-38 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 285 Figure 6-37: Delete Confirmation Message Press [OK] to delete the data under and between the 2 cursors. Press [Cancel] to cancel the deletion. NOTE: The data cannot be recovered once deleted. Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-39...

  • Page 286: Cal. History

    If [Cal. History] keypad is not displayed on the LCD, press [More] to change the displayed root menu. The calibration history will appear. Refer to Chapter 7: Calibration for detail of the calibration history. 6-40 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 287: Manual Qc Analysis

    2 . 4 BASO% 2 1 3 177.4 GRAN-X 9 . 8 177.2 GRAN-Y 10.3 44.2 WBC/BA-X P-LCR 24.3 94.7 WBC/BA-Y 43.0 GRAN-Y(W) Graphic Return Figure 6-38a: QC Data Display Screen (L-J Control) Sysmex SF-3000 Operator's Manual -- Revised December 1995 6-41...
  • Page 288 [x10 /uL] 2 5 0 f L 40fL [ f L ] 10.3 [ f L ] P-LCR 24.3 [ % ] QC Data Return Control Data Graphic Display Figure 6-38c: 6-42 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 289 SF CHECK is analyzed and the lot has already expired. This error also occurs when the expiration date is not entered in the control file. NOTE: Refer to Chapter 8: Troubleshooting for QC error information. Sysmex SF-3000 Operator's Manual -- Revised December 1995 6-43...

  • Page 290: Sampler Qc Analysis (L-J)

    NOTE: The QC Data Display screen as shown in Figure 6-38a on page 6-41 will not be displayed for the QC analysis in Auto mode. 6-44 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 291 The "+" or "-" sign in red back-light will be added to the parameter which is outside the control limits. The results are not plotted, or not stored as control data. Sysmex SF-3000 Operator's Manual -- Revised December 1995 6-45...
  • Page 292 SF CHECK is analyzed and the lot has already expired. This error also occurs when the expiration date is not entered in the control file. NOTE: Refer to Chapter 8: Troubleshooting for QC error information. 6-46 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 293: Qc Methods And Applications

    QUALITY CONTROL 1 0 . QC METHODS AND APPLICATIONS Quality Control in the SF-3000 features the commercial control applications X Control or L-J Control, and also X M Control, which uses routine patient samples. 1 0 . 1 X Control X Control corresponds to the X -R Control used in earlier models.

  • Page 294: M Control

    The control program uses the X B algorithm published by Dr. Brian S. Bull. This algorithm is formulated using the three blood cell indices (MCV, MCH and MCHC). X M Control is used in the SF-3000 because of the large number of control parameters (28).
  • Page 295 The smoothing technique uses data from the previous batch to reduce individual data point fluctuations in the current batch. Here is an example of smoothing performed on a batch of 5 samples: Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-49...
  • Page 296 Parameters for QC having the limit#(%) of 0 (zero) do not appear on the QC Charts. However, data in item 6. above, having patient mark limit#(%) value set at 0 (zero) will be included in X M computation. 6-50 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 297: How Target Values Are Calculated

    RBC control data. The variable target N =2, UL-TARGET=0.11 remains the same, because the denominator (N-1) in the equation 4.61 X/N-1 is 1 in this instance. 4.50 4.39 Figure 6-44: Second Control Value Sysmex SF-3000 Operator's Manual -- Revised September 1995 6-51...

  • Page 298: Variable Target Function And Erroneous Data

    "Check QC Chart" will be displayed, but the data will be entered on the chart. If the control data is 5.61 million however, it will not be entered on the QC Chart, and the message "Reanalyze sample" will be displayed. 6-52 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 299: Control Chart Definition

    QUALITY CONTROL 1 2 . CONTROL CHART DEFINITION In the SF-3000, the Levy-Jennings Method is used as a basic principle for reading the QC Charts. This method consists of the following operation: If the latest control data point is outside the control limits, the last 20 (including the latest) data points are searched for a point that is outside the same limit as the latest data point.
  • Page 300 CHAPTER 7 CALIBRATION INTRODUCTION ............... 7-1 1.1 Calibration Implementation Standards ........7-1 1.2 Calibration Samples ............7-2 1.3 Reference Values ............7-2 HGB/HCT AUTO..............7-3 2.1 Overview of the HGB/HCT Auto Program ......7-3 2.2 Setting (Entering Reference Values)........7-6 2.3 Analyze (Analyzing)............

  • Page 301: Chapter 7 Calibration

    "as needed" basis, and maintain good QC practices, to ensure the accuracy of the system. This chapter covers the process and procedures necessary to calibrate the instrument for HGB (hemoglobin) and HCT (hematocrit). Calibration of the SF-3000 can be performed either automatically or manually. 1 . 1 Calibration Implementation Standards Calibration is not required at specified periodic intervals.

  • Page 302: Calibration Samples

    HCT value : Microhematocrit Method NOTE: • HGB reference value will be influenced by WBC count, diluent, lyse reagent, HGB light source, temperature, etc. • HCT reference value decrease significantly due to excessive anticoagulant. Sysmex SF-3000 Operator's Manual --- Revised September 1995...

  • Page 303: Hgb/Hct Auto

    Next No. 1234567890123 Sysmex Sampler DP:1234567890123 XM HC GP DP SU CALIB. Input Password Password * * * ENTER HGB/HCT HGB/HCT Cal. Return CANCEL Auto Manual History Figure 7-2: Password Input Screen Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 304 Data from the difference between reference values and analyzed samples is utilized. Refer to Section 2.5 Exe.Cal. [Graphic] : Displays the scattergrams and particle size distributions of the last sample analyzed. Refer to Section 2.6 Graphic. Sysmex SF-3000 Operator's Manual --- Revised September 1995...
  • Page 305 Exit Auto Cal. OK? Cancel Figure 7-4: Confirmation Message Window Pressing [OK] erases all data and ends the HGB/HCT Auto program. Pressing [Cancel] retains the calibration data generated, permitting HGB/HCT Auto program to continue. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 306: Setting (Entering Reference Values)

    Use the numerical keys to enter the HGB/HCT reference values obtained using the reference methods. The input format should correspond with the unit settings. Press [Enter] to move the cursor to the next item. NOTE: Press the [C] key to delete the entered values. Sysmex SF-3000 Operator's Manual --- Revised September 1995...
  • Page 307 Exe. Cal. Graphic Return Figure 7-7: HGB/HCT Auto - Setting Screen (3) NOTE: To update the [Current] compensation factor with the average found in the "Compensation (%)" column, use the "Exe.Cal" program. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 308: Analyze (Analyzing)

    CALIBRATION 2 . 3 Analyze (Analyzing) The SF-3000 uses the HGB/HCT Auto submenu program "Analyze" to analyze the reference samples in the Manual mode. From the HGB/HCT Auto submenu, press [Analyze]. "Analyze" display will be backlit in blue, the main unit READY LED will light and the system will be ready to process samples.
  • Page 309 Analyze Graphic Return Exclude Exe. Cal Figure 7-9: Sample Analysis Screen (2) NOTE: To update the [Current] compensation factor with the average found in the "Compensation (%)" column, use the "Exe.Cal" program. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 310: Exclude (Excluding And Recovering Data)

    3 9 . 9 1 0 0 . 1 1 0 0 . 0 [ Current ] 98.1% HCT 100.1% Setting Analyze Graphic Return Exclude Exe. Cal Figure 7-11: Excluding Data Screen (2) 7-10 Sysmex SF-3000 Operator's Manual --- Revised September 1995...
  • Page 311 3 9 . 9 1 0 0 . 1 1 0 0 . 0 [ Current ] 98.1% HCT 100.1% Setting Analyze Exe. Cal Graphic Return Exclude Figure 7-13: Recovering Data Screen (2) Sysmex SF-3000 Operator's Manual -- Revised September 1995 7-11...

  • Page 312: Exe. Cal. (Calibrating)

    HGB/HCT Auto program to continue without updating the calibration values. NOTE: "Exe.Cal" can be executed only once. However, it can be executed again after a reference value is changed, reanalysis is performed or data is excluded. 7-12 Sysmex SF-3000 Operator's Manual --- Revised September 1995...
  • Page 313 If the difference continues to exceed the limits, check for sample abnormalities such as coagulation problems, abnormal morphology, patient medication, old blood samples, etc. If no sample abnormalities are discovered, contact your Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995 7-13...

  • Page 314: Graphic (Displaying Graphs)

    [ f L ] P-LCR 24.3 [ % ] Return Figure 7-15: Graphic Display Screen Press [Return] key and the instrument returns to the HGB/HCT Auto screen, permitting Auto Cal. to continue. 7-14 Sysmex SF-3000 Operator's Manual --- Revised September 1995...

  • Page 315: Hgb/Hct Manual

    HCT values. Thoroughly mix the same samples, then analyze them in the Manual mode. If there is a difference between the data measured with the SF-3000 and the reference values measured with the reference methods, calculate new compensation factors by using the following formula:...

  • Page 316: Executing The Hgb/Hct Manual Program

    XM HC GP DP SU < HGB/HCT Manual > CALIB. Calibration 98.4 ↑ 100.0 ↓ Edit Cancel HGB/HCT HGB/HCT Cal. Return Auto Manual History Figure 7-16: HGB/HCT Manual Calibration Screen (1) 7-16 Sysmex SF-3000 Operator's Manual --- Revised September 1995...
  • Page 317 If the difference continues to exceed the limits, check for sample abnormalities such as coagulation problems, abnormal morphology, patient medication, old blood samples, etc. If no sample abnormalities are discovered, contact your Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995 7-17...

  • Page 318: Calibration History

    9 9 . 4 1 0 1 . 0 1995/10/31 13:45 1 0 0 . 2 99.8 ↑ ↓ Delete Cancel HGB/HCT HGB/HCT Cal. Return Auto Manual History Figure 7-18: Calibration History Screen 7-18 Sysmex SF-3000 Operator's Manual --- Revised September 1995...

  • Page 319: Deleting The Cal. History

    Figure 7-19: Deletion Confirmation Window Pressing [Cancel] returns to the Calibration History display without deleting the calibration history data. Pressing [OK] deletes the history. NOTE: The latest calibration history cannot be deleted. Sysmex SF-3000 Operator's Manual -- Revised September 1995 7-19...

  • Page 320: Outputting The Cal. History

    To obtain the printout of the calibration history, use the "Hard Copy" program as below. Display the calibration history on the LCD. Press the [Sysmex] keypad in the upper left corner of the screen. The Sysmex submenu window will appear.
  • Page 321 CHAPTER 8 TROUBLESHOOTING INTRODUCTION ...............8-1 TROUBLESHOOTING BY ERROR MESSAGES ......8-2 2.1 Alphabetical Error Message Index........8-2 2.2 Contents (Error Messages By Function)........8-5 2.3 Troubleshooting Guide ...........8-9 TEST PROGRAMS............8-59 3.1 Executing a Test Program ..........8-59 3.2 Test SRV ..............8-62 3.3 Test Motor..............
  • Page 322 TROUBLESHOOTING INTRODUCTION This chapter contains explanations of the error messages displayed by the SF-3000, when malfunctions occur, and of the countermeasures that the operator can take. Should the countermeasures given in this chapter fail to restore the system to normal condition, contact your Sysmex service representative.
  • Page 323 Hand Lower Position Error .............8-32 Hand Move Position Error ..............8-31 Hand Upper Position Error .............8-32 HC Communication Error...............8-50 HGB Drain Error.................8-42 HGB Error (Blank or Sample)............8-42 ID Read Error................8-48 ID Unit Com. Error ..............8-44 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 324 RBC Detector Temp High .............. 8-11 RBC Detector Temp Low............... 8-11 RBC Fast To Start ............... 8-37 RBC Sampling Error ..............8-40 RBC Slow To Start..............8-36 React Chamber Thermal Sensor Discon..........8-13 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 325 WBC Channel Error ..............8-43 WBC Detector Temp High ..............8-13 WBC Detector Temp Low...............8-13 WBC Detector Thermal Sensor Discon..........8-14 WBC/BASO CCSD Noise Error ............8-41 WBC/BASO Sampling Error ............8-40 Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised February 1997...
  • Page 326 Waste Chamber 2 Not Draining............8-16 Waste Chamber 3 Not Draining............8-16 Motor Errors Sheath Motor Function Error............8-18 Rinse Motor Function Error ............8-19 WB Asp Motor Function Error............8-20 Mixing Motor Speed Error.............. 8-20 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 327 Set Manual Piercer Cover ...............8-35 Manometer Errors RBC Slow To Start ..............8-36 RBC Count Too Long ..............8-36 RBC Fast To Start................8-37 RBC Count Too Short..............8-37 Close RBC Detector Cover..............8-37 Close WBC Detector Cover.............8-37 Laser Power Error................8-38 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 328 ACK Code Error................. 8-49 ACK Time Out Error ..............8-49 HC Communication Error .............. 8-50 Not Assigned................8-50 Not Transmissible ............... 8-51 Off Line ................... 8-51 Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised February 1997...
  • Page 329 < Control Expired >..............8-55 < QC Analysis Error >..............8-56 1 6 . Maintenance Instruction Messages Clean the Orifice................8-57 Clean the SRV ................8-57 Clean the Waste Chamber ...............8-57 Clean the WBC Detector..............8-58 Replace Piercer ................8-58 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 330 1) Adjust 0.6 kg/cm pressure. (See Chapter 9, Section 4: Main Unit Adjustment .) 2) If adjustment of 0.6 kg/cm pressure failed, the regulator is probably defective. Contact your Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 331 4) Check the vacuum output by pinching the tubing between the Main Unit and the Pneumatic Unit. If the Pneumatic Unit vacuum returns to normal, problem is in Main Unit. 5) Clean the vacuum orifice. (See Chapter 5, Section 5.1: Clean Orifice .) 8-10 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 332 Sample Rack. Upon correcting the error the operator will need to reset the rack. 7) If the error persists after the power ON, contact Sysmex service representative. Temperature Errors...

  • Page 333: Reaction Chamber Temp High

    2) Thermistor is defective. Corrective Action 1) Wait until temperature has stabilized to the required range. If this error is still displayed 30 minutes after the power on, contact Sysmex service representative. Sheath Heater Temp High Sheath Heater Temp Low Phenomena Temperature of Sheath Heater is outside the required range.
  • Page 334 1) The temperature in the unit has not reached the required range after power 2) Thermistor is defective. Corrective Action 1) Wait until temperature has stabilized to the required range. If this error is still displayed 30 minutes after the power on, contact Sysmex service representative. React Chamber Thermal Sensor Discon. Phenomena The thermal sensor monitoring the WBC reaction chamber is defective.
  • Page 335 1) In the auto mode analysis, reset the rack and press [Sampler], then [Start] to resume analysis. 2) If the error persists, the thermal sensor replacement may be necessary. Contact your Sysmex service representative. 8-14 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 336: Replace Reagent Container Fba

    If there is abnormality, repair or replace the affected items. 3) If the error persists, contact Sysmex service representative. Only when it is requested by the service representative, open the left side cover and check the float switch(es) and tubing visually with his/her assistance.
  • Page 337 Refer to Chapter 5, Section 5.2: Execute Clean Waste Chamber Sequence . 2) If the error persists, contact Sysmex service representative. Only when it is requested by the service representative, open the left side cover and check the float switch(es) and tubing visually with his/her assistance.
  • Page 338 Wear rubber gloves when checking the hydraulic system. Wash your hands with an antiseptic solution after completing the procedure. CAUTION: DO NOT open the Main Unit left side cover without direction or assistance of Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-17...
  • Page 339 2) If the error persists after the test program is completed, turn the Main Unit power OFF, then ON again. 3) If the error still persists, contact your Sysmex service representative. Only when it is required by the service representative, open the left side...

  • Page 340: Rinse Motor Function Error

    3.3: Test Motor in this chapter.) 3) If the error persists after the test program is completed, turn the Main Unit power OFF, then ON again. 4) If the error still persists, contact your Sysmex service representative. Rinse Motor Manual Rinse Cup...
  • Page 341 3.3: Test Motor in this chapter.) 2) If the error persists after the test program is completed, turn the Main Unit power OFF, then ON again. 3) If the error still persists, contact your Sysmex service representative. Mixing Motor Speed Error Phenomena WBC reaction chamber mixing motor does not move properly.
  • Page 342 Blood aspiration sensor, that monitors whether blood is being aspirated normally during sampler operation, is defective. Corrective Action 1) Replace the blood aspiration sensor. Contact your Sysmex service representative to replace it. 2) As a tentative measure, change the Sampler Stop Conditions to allow sampler operation to continue.

  • Page 343: Sample Not Asp Error

    (d) If the error still persists, it is assumed that the piercer is blocked by clots. Replace the piercer. Refer to Chapter 5, Section 8.2: Replace the Piercer . 8-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 344 In such cases, aspiration is performed anyway, and if the volume is insufficient, "Short Sample" or "Sample Not Asp Error" will occur. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-23...
  • Page 345 3) Clean the SRV. (Refer to Chapter 5, Section 5.3: Clean SRV .) 4) If the error persists, turn the Main Unit power OFF, then ON again. 5) If the error still persists, contact Sysmex service representative. 8-24 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 346 1) Install the piercer unit cover. 2) Perform the CP mechanism operation test. Refer to Section 3.6: Test Sampler in this chapter. 3) If error persists, contact your Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-25...
  • Page 347 3) If the error persists after the test program is completed, turn the Main Unit power OFF, then ON again. 4) If the error still persists, contact your Sysmex service representative. 8-26 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 348 2) Apply lubricant to bearing. 3) Reset the rack in the right rack pool and press [Sampler], then [Start] key to resume the analysis. 4) If the error persists, contact your Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-27...
  • Page 349 2) Apply lubricant to bearing. 3) Reset the rack in the right rack pool and press [Sampler], then [Start] key to resume the analysis. 4) If the error persists, contact your Sysmex service representative. 8-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 350 2) Rack position sensor(s) are defective. Corrective Action 1) Reset the rack and press [Sampler], then [Start] key to resume the analysis. 2) If the error still persists, contact your Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-29...
  • Page 351 Corrective Action 1) The Reset the rack and press [Sampler], then [Start] key to resume the analysis. 2) If the error still persists, contact your Sysmex service representative. Rack Move Error 3 Phenomena A sample rack moved in the measurement line when STAT ready.
  • Page 352 2) Foreign object is in the left rack pool, blocking movement of the rack. 3) Reset the rack and press [Sampler], then [Start] key to resume the analysis. 4) If the error still persists, contact your Sysmex service representative. Hand Initial Position Error Hand Move Position Error Phenomena Hand clipper did not move forward and backward correctly.
  • Page 353 3.6: Test Sampler in this chapter.) 5) If the error persists after the test program is completed, turn the Main Unit power OFF, then ON again. 6) If the error still persists, contact your Sysmex service representative. Tube Invert Position Error Phenomena The collection tube was not inverted correctly for piercing.
  • Page 354 4) If the error persists after the test program is completed, turn the Main Unit power OFF, then ON again. 5) If the error still persists, contact your Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-33...

  • Page 355: Sampler Start Error (Blood Sensor)

    Corrective Action 1) Remove the racks or any foreign objects from the left rack pool. 2) Remove dirt. 3) If the error still persists, contact your Sysmex service representative. Sampler Start Error (Blood Sensor) Sampler Start Error (Sensor 4) Sampler Start Error (Sensor 5)

  • Page 356: Set Manual Piercer Cover

    Sensor switch to detect closed lid is defective. Corrective Action 1) Make sure to close the piercer lid. 2) If the error persists, contact your Sysmex service representative. Set Manual Piercer Cover Phenomena Manual CP cover is not installed when the Start Switch is pressed.
  • Page 357 3) Check and adjust 250 mmHg vacuum if required. Refer to Chapter 9, Section 4: Main Unit Adjustment . 4) Clean vacuum orifice. Refer to Chapter 5, Section 5.1: Clean Orifice . 5) If the error persists, contact your Sysmex service representative. 8-36 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 358 2) Check that sample is reaching the RBC transducer chamber. 3) Check and adjust 250 mmHg vacuum if required. Refer to Chapter 9, Section 4: Main Unit Adjustment . 4) If the error persists, contact your Sysmex service representative. Close RBC Detector Cover Close WBC Detector Cover Phenomena RBC and/or WBC detector cover is open.
  • Page 359 Corrective Action 1) Check the laser output operation using laser test program. (Refer to Section 3.12: Test Laser Unit in this chapter.) 2) Replacement of laser unit is necessary. Contact your Sysmex service representative. Analysis Errors < Data Error >...
  • Page 360 2) Clean the SRV. Refer to Chapter 5, Section 5.3: Clean SRV . 3) Execute a background check by pressing [Auto Rinse] key in the root menu. 4) Replace the reagent. Refer to Chapter 5, Section 8.1: Replace Reagent . Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-39...
  • Page 361 4) If the error persists, clean the RBC transducer and aperture. Refer to Chapter 5, Section 7.3: Execute Clean RBC Transducer Sequence , and to Chapter 5, Section 7.4: Clean RBC Detector Aperture . 5) If the error still persists, contact the Sysmex service representative. DIFF Sampling Error WBC/BASO Sampling Error...
  • Page 362 1) Move the source of external noise (such as radio, centrifuge, temperature controlled water bath, etc.) well away from the instrument. 2) Reanalyze the sample, if required. 3) If the error persists, contact your Sysmex service representative. Low Count Error Phenomena...
  • Page 363 Chamber and Manometer (Shutdown) . 2) Perform a background check by pressing [Auto Rinse] keypad in the root menu. 3) If the error persists, contact Sysmex service representative. HGB Drain Error Phenomena The HGB flow cell cannot be drained completely or drained slowly.

  • Page 364: Short Sample

    PDA PCB in this chapter.) 4) If the error persists after the test program is completed, turn the Main Unit power OFF, then ON again. 5) If the error still persists, contact your Sysmex service representative. WBC Channel Error Phenomena The particles counted by WBC/BASO Channel are differed not less than two times of that by DIFF Channel.
  • Page 365 1) Move the source of external noise (such as radio, centrifuge, temperature controlled water bath, etc.) well away from the instrument. 2) Turn the Main Unit power OFF, then ON again. 3) If the error persists, contact Sysmex service representative. Sampler Com. Error ID Unit Com. Error...
  • Page 366 1) Check the Stored Data using test program. Refer to Section 3.8: Test Memory in this chapter. 2) If any item results in NG, contact Sysmex service representative. 3) Turn the Main Unit power OFF. Verify that the battery is connected correctly (see Appendix B: Installation ).
  • Page 367 1) Check and set the program version using version check program. Refer to Section 3.13: Version Check in this chapter. 2) Turn OFF, then ON the Main Unit power. 3) If the error persists after power ON, contact Sysmex service representative. 8-46...

  • Page 368: Stored Data Error

    Memory in this chapter. 3) If the error persists after the test program is completed, turn the Main Unit power OFF, then ON again. 4) If the error still persists, contact Sysmex service representative. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-47...

  • Page 369: Stored Data Full

    ID bar code label specification. 2) Correct the sample ID number, if necessary. 3) Reset the rack and press [Sampler], then [Start] key to start analysis. 4) If the error persists, contact Sysmex service representative. 8-48 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 370 The HC setting is not correct. Corrective Action 1) Check the HC settings. Refer to Chapter 11, Section 5.1: HC Setting . 2) If the error persists, contact Sysmex service representative. ACK Time Out Error Phenomena Indicates that the ACK signal cannot be received from the host computer during a specified time period after the data has been transferred.
  • Page 371 3) Set the host computer connection to "Not Connected", then reset to "Connected". Refer to Chapter 11, Section 5.1: HC Setting . 4) If the error persists, contact Sysmex service representative. Not Assigned Phenomena The output to HC is assigned when the host computer is not specified for connection.
  • Page 372 2) Check the HC status in the Status Display by pressing [Status] key in the root menu. 3) Check that the interface cable is securely connected. 4) If the error persists, contact Sysmex service representative. Off Line Phenomena The output to HC is assigned when the DSR signal from the host computer is OFF.
  • Page 373 3) Set the GP status to "Connected". Refer to Chapter 11, Section 5.2: GP Setting . 4) If the error persists, contact Sysmex service representative. GP Off Line Phenomena The output to GP is assigned when the printer is in the off line condition or when a printer error occurs.
  • Page 374 Corrective Action 1) Set the GP or DP to "Connected". Refer to Chapter 11, Section 5.2: GP Setting or Section 5.3: DP Setting for the procedures. 2) If the error persists, contact Sysmex service representative. Off Line/Device Error Phenomena The output to GP or DP is assigned when the printer is in the off line condition or when a printer error occurs.
  • Page 375 2) The interface cable is disconnected. Corrective Action 1) Power ON the printer. 2) Verify that the interface cable is securely connected. 3) If the error persists, contact Sysmex service representative. 8-54 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 376 Refer to Chapter 6: Quality Control. 2) Check the control sample or SF CHECK for any signs of abnormality. 3) Calibrate the instrument, if required. Refer to Chapter 7: Calibration. 4) If the error persists, contact Sysmex service representative. < Control Expired > Phenomena Control blood which has been expired is analyzed.
  • Page 377 Rack No. and the Tube Pos. No. for the next sample. Press [Sampler], then [Start] key to resume the sampler operation. 3) If the error persists, contact Sysmex service representative. 8-56 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 378 1) Clean the Waste Chamber. Refer to Chapter 5, Section 5.2: Execute Clean Waste Chamber Sequence . 2) Reset the Waste Chamber counter. Refer to Chapter 5, Section 9: Reset the Counter. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-57 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 379 1) Replace the piercer. Refer to Chapter 5, Section 8.2: Replace the Piercer . 2) Reset the Piercer counter. Refer to Chapter 5, Section 9: Reset the Counter. 8-58 Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 380 TROUBLESHOOTING TEST PROGRAMS The SF-3000 is equipped with test programs for checking system operation and finding the causes of abnormalities detected in the SF-3000 system. 3 . 1 Executing a Test Program Press [Mainte.] keypad in the root menu. The Maintenance second menu will be displayed.
  • Page 381 Rack Feed Out Sheath Motor LCD Display Cancel Rinse Motor Sampler CP Mech. Touch Panel Mixing Motor ID Read DP Print GP Print Laser Return Figure 8-7: Test Execution Message Window 8-60 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 382 If you attempt to run these programs during analysis, a "Wait until Ready" message will appear, and the programs will not be executed. Also note that analysis is not possible while test programs are in the progress. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-61...
  • Page 383 When operation ends, the valve will return to the initial position. Rotor Valve Initial Position Counterclockwise Figure 8-8: Sample Rotor Valve Operation Press [Return] to end the test program. The system automatically performs auto rinse to clean the hydraulic lines. 8-62 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 384 Lower End Figure 8-9: WB Aspiration Pump Operation NOTE: Only when it is required by Sysmex service representative, open the Main Unit front panel behind the Sampler Unit (if installed), and observe the operation of Whole Blood Aspiration Pump with his/her assistance.
  • Page 385 When operation ends, the message disappears. The WB pump will return to the initial position. Press [Return] to end the test program. The system automatically performs auto rinse to clean the hydraulic lines. 8-64 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 386 Lower End (Initial Position) Figure 8-10: Sheath Injector Piston Operation NOTE: Only when it is required by Sysmex service representative, open the Main Unit left side cover and observe the operation of Sheath Injector Piston with his/her assistance. CAUTION: DO NOT open the left side cover without instruction and assistance of Sysmex service representative to observe Sheath Injector Piston operation.
  • Page 387 When operation ends, the message disappears. The piston will return to the initial position. Press [Return] to end the test program. The system automatically performs auto rinse to clean the hydraulic lines. 8-66 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 388 When operation ends, the message disappears. The mechanism will return to the initial position. Press [Return] to end the test program. The system automatically performs auto rinse to clean the hydraulic lines. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-67...
  • Page 389 Mixing Motor DP Print ID Read GP Print Laser Return Figure 8-12: Mixing Motor Operation Speed Press [Cancel] to end the Mixing Motor test program. Press [Return] to end the Test program. 8-68 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 390 RDW-SD ---.- [fL] PLT Flag RDW-CV ---.- ---- [x10 / u L ] 2 5 0 f L 40fL ---.- [fL] ---.- [fL] P-LCR ---.- Return Figure 8-13: PDA Check Screen Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-69...
  • Page 391 During the test operation, the message "Executing Laser test. Please wait." is displayed. When the Laser Test completes without any abnormality detected, the system returns to Ready status. Press [Return] to end the test program. 8-70 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 392 Bring the Test screen by pressing [Mainte.] and then [Test] keypads (Refer to Section 3.1 in this chapter). Press the [Rack Shift] keypad from the Test screen. The message "Set Rack and Press Start Switch" will appear. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-71...
  • Page 393  Press the Start Switch. Rack feed out operation will begin. Check the sampler operation. Rack Feed out Figure 8-16: Rack Feed Out Operation Press [Return] to end the test program. 8-72 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 394 Check the operation using the figure below as a guide. Figure 8-17: CP Mechanism Operation Press [Return] to end the test program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-73 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 395 1 2 3 4 5 6 7 8 9 0 1 3 1 1 2 3 4 5 6 7 8 9 0 1 3 2 Return Figure 8-18: ID Read Display Press [Return] to end the test program. 8-74 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 396 Figure 8-19: Stored Data Check Window "OK" indicates that data is normal. "NG" indicates that there is an abnormality in the data. If "NG" is displayed, contact your Sysmex service representative. Press [OK] key to end the Memory Test program.
  • Page 397 A floppy disk can be formatted using "Mainte." - "Format FD" program. Refer to Chapter 5, Section 10: Format FD . If the disk is normal, the display will return to the previous screen. 8-76 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 398 A full floppy disk or unformatted floppy disk is assumed as abnormal and the message "FD format NG" will be displayed. Press [OK] to end the FD Test program. Press [Return] to end the Test program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-77...
  • Page 399 After the display becomes white the second time, the test program ends automatically and the Test screen will appear. NOTE: This program cannot be stopped halfway. Press [Return] to end the Test program. 8-78 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 400 Press any keypad to test its input. The pressed keypad will be backlit in light blue. Press [Return] to end the Touch Panel Test program. Press [Return] to end the Test program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-79...
  • Page 401 Bring the Test screen by pressing [Mainte.] and then [Test] keypads (Refer to Section 3.1 in this chapter). Press the [DP Print] keypad from the Test screen. The Data Printer will print out a dummy data. Figure 8-23: DP Test Print Example 8-80 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 402 Press the [GP Print] keypad from the Test screen. The Graphic Printer will print out a dummy data. <WBC> <RBC> <PLT> Figure 8-24: GP Test Print Example NOTE: Scattergrams are not printed. Sysmex SF-3000 Operator's Manual -- Revised October 1995 Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-81...
  • Page 403 Press the [HOST Interface] keypad from the Test screen. A dummy data will be transmitted to the Host Computer. HC Test Dummy Data (234 bytes including STX and ETX) D1U05259500000000000 TEST PRINT00000000000000000 00111110222203333044440555506666077770888801111022 22033330444405555099999011111022222033333044444077 77088880444405555066660000000000000000000000000000 00000000000000000000000000000000 Figure 8-25: Host Computer Output Example 8-82 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 404 The Version Check screen will appear. All the program versions will be displayed. The system reads and displays the current check sum values. If the read values match the set values, "Complete" appears on the right of the values. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-83...
  • Page 405 Ver. 00-00 ] [ 3SF31 Ver. 00-00 ] Complete Sampler Unit [ 4SF31 Ver. 00-09 ] [ 4SF31 Ver. 00-09 ] Complete Execute settings. Continue Cancel Quit Figure 8-29: Version Check Confirmation Window 8-84 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 406 Select [Service Data] from the maintenance second menu. The first page screen WBC data for the latest sample will be displayed. Use [←] and [→] keys to display "P2. RBC/PLT" or "P3. Counters". Press [Return] to exit the program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-85...
  • Page 407 MONO area in WBC/BASO X-axis channel. (Unit: fL) LYMPH/MONO-Y : Most frequently occurring value for the LYMPH/ MONO area in WBC/BASO Y-axis channel. (Unit: fL) 8-86 Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 408 8 2 0 7 7 9 5 8 7 (x 1) (x 1) ← → Return Figure 8-32: Service Data - RBC/PLT Screen Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-87 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 409 8 ) PLT Distribution This item is marked with a cell size abnormality indicator if the PLT size distribution is abnormal. Refer to Chapter 10, Section 7.4 Histogram Flags for the indicators. 8-88 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 410 Figure 8-34: Counter Reset Confirmation Screen Press [Continue] to return to the Counter screen. Press [Set] to reset the counter. Press [Cancel] to cancel the counter reset and exit the Service Data program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-89...
  • Page 411 Press [Status Display] keypad in the root menu. The first page screen "Analyzer Condition" will be displayed. Use [←] and [→] keys to display "P2. Analysis Process" or "P3. SV/Sensor Status". Press [Return] to exit the program. 8-90 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 412 : "Not Assigned", "Power/Cable OFF", "Off Line", "Paper Empty", "Device Error" [GP] : "Not Assigned", "Power/Cable OFF", Off Line/Dev.Error", "Paper Empty" [HC] : "Not Assigned", "Off Line", "Ack Time Out", "Ack Code Error", "Not Transmissible" Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-91...
  • Page 413 DIFF Count RBC Analyze WBC/BASO Analyze DIFF Analyze Edit Store : Entry : Run : Finish Normal Interruption : Finish Abnormal ← → Return Figure 8-35: Status Display - Analysis Process Screen 8-92 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 414 WBC Detector : 35.0 °C Press 0.5k : 0.510kg/cm2 React Chamber : 30.0 °C Vacuum 250 : 250 mmHg Sheath Reagent ← → Return Figure 8-36: Status Display - SV/Sensor Status Screen Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-93...
  • Page 415 This program displays the list of past 189 errors and recovery messages. Use this program to display corrective action messages for the error messages. NOTE: Analysis Error messages are not displayed on the Error Log screen. Press the [Sysmex] keypad in the left upper corner of the screen. Ready Manual Mode Next No.1234567890123...
  • Page 416 Down Corrective Action 1) Replace the reagent. 2) Check the float switch. Cancel 3) Check the hydraulic system. Info Return Error Information Screen Figure 8-39: Press [Return] to exit the program. Sysmex SF-3000 Operator's Manual -- Revised September 1995 8-95...
  • Page 417 CHAPTER 9 ADJUSTMENT INTRODUCTION ............... 9-1 PRESSURE/VACUUM DISPLAY..........9-1 2.1 Pneumatic Gauges............9-1 2.2 Displaying the Monitored Values on the LCD......9-2 PNEUMATIC UNIT ADJUSTMENT ........9-3 3.1 Adjusting the 2.0 kg/cm ..........9-4 3.2 Adjusting the Vacuum............. 9-5 MAIN UNIT ADJUSTMENT..........9-5 4.1 Adjusting to 0.6 kg/cm ..........

  • Page 418: Chapter 9 Adjustment

    ADJUSTMENT INTRODUCTION From time to time it may be necessary to adjust certain components of the SF-3000 system. The Sysmex representative will perform initial adjusting; following that, the customer should adjust the instrument as described in the procedures provided in this chapter.

  • Page 419: Displaying The Monitored Values On The Lcd

    0.6k : 0.605kg/cm2 WBC Detector : 35.0 °C Press 0.5k : 0.510kg/cm2 React Chamber : 30.0 °C Vacuum 250 : 250 mmHg Sheath Reagent ← → Return Figure 9-2: SV/Sensor Status Display Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 420: Pneumatic Unit Adjustment

    , check for the following items and adjust as necessary. • Leaks or cracks in connectors • Blockage or pinching in pneumatic lines 2.0 kg/cm Regulator Figure 9-3: 2.0 kg/cm Regulator (Pneumatic Unit - Front) Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 421: Adjusting The 2.0 Kg/Cm

    NOTE: The required range for normal operation is within 1.8-2.3 kg/cm When completing the adjustment, tighten the lock screw on the top of the regulator knob. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 422: Adjusting The Vacuum

    As instrument usage increases, the vacuum gradually decreases. In such cases, the vacuum pump and/or compressor gaskets may need to be repaired or replaced. Contact a Sysmex service representative. MAIN UNIT ADJUSTMENT Adjust the pressure and vacuum in the Main Unit to 0.6 kg/cm , 0.5 kg/cm...

  • Page 423: Adjusting To 0.6 Kg/Cm

    ± NOTE: The required range for normal operation is within 0.6 0.03 kg/cm Press the knob in. This locks the regulator. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 424: Adjusting To 0.5 Kg/Cm

    ± NOTE: The required range for normal operation is 0.5 0.03 kg/cm Press the knob in. This locks the regulator. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 425: Adjusting The 250 Mmhg Vacuum

    The required range for normal operation is 250 ± 20 mmHg. NOTE: After completing the adjustment, re-tighten the lock nut. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 426: Lcd Contrast Adjustment

    Decrease Increase Figure 9-11: Contrast Controller Turn the control knob. Turning the knob counterclockwise (when viewed from the knob top) increases the contrast. Release the stop bar and close the front cover. Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 427: Id Bar Code Reader Adjustment (Option)

    ADJUSTMENT ID BAR CODE READER ADJUSTMENT (Option) NOTE: When adjusting the ID Bar-Code Reader, contact your Sysmex service representative for his/her instruction and assistance. Pull out the Sampler Unit by approx. 10 cm, and remove the Main Unit Front Panel-B Assy.
  • Page 428 When the adjustment is complete, pull out the Sampler Unit by approx. 10 cm, and refit the Front Panel-B Assy. Refit the Sampler Unit. CAUTION: When refit the Sampler Unit, take care not to pinch the tubing. Sysmex SF-3000 Operator's Manual -- Revised September 1995 9-11...
  • Page 429 CHAPTER 10 FUNCTIONAL DESCRIPTION INTRODUCTION ............. 10-1 DETECTION PRINCIPLES..........10-2 2.1 Flow Cytometry by Semiconductor Laser......10-2 2.2 DC Detection Method ........... 10-3 2.3 SLS-Hemoglobin............10-3 SAMPLE FLOW BLOCK DIAGRAM........10-4 RBC/PLT/HGB ANALYSIS ..........10-5 4.1 RBC/PLT Analysis Flow..........10-5 4.2 HGB Analysis Flow ............

  • Page 430: Chapter 10 Functional Description

    FUNCTIONAL DESCRIPTIONS INTRODUCTION This chapter explains the detection principles, analysis methodology, individual analysis procedures, and hardware components employed by the SF-3000. • Detection Principles Describes flow cytometry by means of semiconductor laser, DC detection method, and the SLS-hemoglobin method. •...

  • Page 431: Detection Principles

    Sample Flow High-Angle Scattered Light Receiver (Photo Diode) Collector Lens Semiconductor Laser Beam Stopper Low-Angle Scattered Light Receiver (Photo Diode) Collimator Lens Flow Cell Condenser Lens Figure 10-2: Scattered Light Measurement System 10-2 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 432: Dc Detection Method

    Conventionally, the most common automated method of measuring hemoglobin is the cyanmethemoglobin method. This method has both advantages and disadvantages when used with a fully automated instrument such as the SF-3000. The cyanmethemoglobin method was recommended as the international standard in 1966 by the ICSH (International Committee for Standardization in Haematology).

  • Page 433: Sample Flow Block Diagram

    1:500 dilution in 1.96 mL 40.0 µL CELLPACK RBC Mixing Chamber 3.0 µL 0.997 mL Analyzed 1:500 of diluted SULFOLYSER sample in HGB Flow Cell 0.5 mL Figure 10-5: RBC/PLT/HGB Sample Flow 10-4 Sysmex SF-3000 Operator's Manual -- Revised October1995...

  • Page 434: Rbc/Plt/Hgb Analysis

    RBC/PLT detector. The RBC detector counts RBC and PLT by means of the DC detection method. At the same time, HCT (hematocrit) is measured by the cumulative pulse height detection method. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-5...

  • Page 435: Hgb Analysis Flow

    The concentration of the SLS- hemoglobin is measured by its absorbance of light, as compared to the absorbance of the diluent (blank) measured before the sample entered the flow cell. 10-6 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 436: Red Blood Cell (Rbc) Indices

    MCH (pg) = x 10 RBC (x10 /µL) Mean Corpuscular Hemoglobin Concentration (MCHC) MCHC is calculated from HGB and HCT as follows: HGB (g/dL) MCHC (g/dL) = x 100 HCT (%) Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-7...

  • Page 437: Wbc Classification

    White blood cells (WBC) are generally classified as lymphocytes, monocytes, and granulocytes. Granulocytes are further subclassified as neutrophils, basophils, eosinophils. The SF-3000 uses the optical detector block incorporating the semiconductor laser to analyze the WBC population. This section explains the applicable analysis procedures.

  • Page 438: Wbc/Baso Analysis

    The sheath injector piston slowly sends 30.4 µL of 1:30 diluted sample to the WBC/BASO detector; then the system, using the flow cytometry by means of semiconductor laser, performs the analysis. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-9...

  • Page 439: Wbc Scattergrams

    DIFF WBC/BASO Low-Angle Scattered Light Information High-Angle Scattered Light Information Figure 10-10: Scattergram Generation The SF-3000 uses two channels, one for 4 DIFF analysis and the other one for WBC/BASO analysis. 10-10 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 440 DIFF WBC/BASO Neutrophils + Basophils Monocytes Basophils Eosinophils Lymphocytes RBC Ghosts Lymphocytes + Monocytes + Neutrophils + Eosinophils Figure 10-11: Cell Distribution Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-11...

  • Page 441: Rbc/Plt Particle Size Distribution Analysis

    The particle size distribution is used to check the discriminator levels for abnormal relative height, the existence of two or more peaks, and the distribution width. The SF-3000 employs the following two methods to show the red blood cell distribution width (RDW).

  • Page 442: Plt Particle Size Distribution

    Food & Drug Administration (FDA). The obtained values should be excluded from all print forms until reporting approval has been obtained. See Chapter 11: System Setup for information on excluding these values. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-13...

  • Page 443: Particle Size Distribution Representation

    The distribution width of the particle size distribution, in particular, can appear completely different, depending on how it is presented or drawn. The SF-3000 employs two representation methods: a Relative Height Method (Normal Expression); and an Absolute Count with Normogram Representation (Dotted Normal Range), developed to permit the user to obtain more information directly from the particle size distribution.

  • Page 444: Histogram Flags

    The relative height at the PLT upper discriminator exceeds the preset limit. The PLT histogram does not cross the 20% height level twice, and the PDW data is "---.-". Two or more PLT populations exist, and the PDW data is "---.-". Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-15...

  • Page 445: Main Unit Electronic System

    Altogether, the data from 23 parameters are displayed on the LCD screen. The instrument can output this data to external devices such as the host computer, data printer, graphic printer, and line printer. 10-16 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 446 ID Bar code reader Operation Unit Interface Touch panel Display Unit interface Power circuit Interface Hard disk drive Interface Floppy disk drive Interface Data Printer Interface Graphic Printer Interface Host Computer Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-17...

  • Page 447: Main Unit Components

    Press this switch to start analyzing in manual mode, capillary mode or closed mode. ( 4 ) ID Bar Code Reader (Optional) A bar code affixed to the collection tube or rack can be read during sampler analysis. 10-18 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 448 QC menus, action messages, etc. Touch panel is incorporated to transmit operational commands to the system and to view any pertinent information. ( 6 ) Ready LED Lights up when the Main Unit is "Ready" or "Sample Asp. Ready". Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-19...

  • Page 449: Rear

    Air Drier Removes dust and moisture from the (pressure side) air supplied by the Pneumatic Unit. ( 6 ) Air Drier Inlet Nipple Connects to the pressure nipple on the Pneumatic Unit. 10-20 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 450 WBC reagent (STROMATOLYSER-FD (II)) is aspirated through this nipple. Connects with the STROMATOLYSER-FD (II) container. ( 1 6 ) Waste Outlet Nipple Waste fluid discharges from this nipple. Can be connected to a sewer system or a waste container. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-21...

  • Page 451: Right Side

    Uses 3.5 inch 2HD (double sided high density) type floppy disks. CAUTION: The floppy disks for the SF-3000 can be used only on this instrument. If the disks are used on other instruments or computers, the data may be unreadable on the SF-3000.
  • Page 452 ( 6 ) Pneumatic Power Outlet Supplies power to the Pneumatic Unit. When the Pneumatic Unit is not operated for preset time period, the Main Unit automatically powers off the Pneumatic Unit. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-23...

  • Page 453: Left Side

    Prevents dust from entering into the bellows unit. ( 4 ) Bellows Unit Adjusts vacuum to 250 mmHg. ( 5 ) Vacuum Orifice Adjusts the vacuum (250 mmHg) supplied to the waste chamber. 10-24 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 454: Front Interior

    SV4, and Master Valve MV1. ( 9 ) Whole Blood Aspiration Pump Aspirates the whole blood sample. ( 1 0 ) Whole Blood Aspiration Motor Drives the whole blood aspiration pump. Sysmex SF-3000 Operator's Manual -- Revised February 1996 10-25...

  • Page 455: Left Interior

    FUNCTIONAL DESCRIPTIONS, Main Unit 9 . 6 Left Interior WARNING! Do not open the Left Side Cover without assistance of Sysmex service representative. (3) WBC Detector Block (4) SheathTemp. Control Unit (2) FDT Reagent Chamber & FDO Reagent Chamber (hidden)
  • Page 456 ( 1 2 ) Waste Chamber 2 Accumulates waste from Reaction Unit, Sheath Charging line, and Sheath Temp. Control Unit. ( 1 3 ) Waste Chamber 3 Accumulates waste from the Sheath Flow Cell. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-27...
  • Page 457 Dispenses aliquot of STROMATOLYSER-FB into the WBC/BASO dilution sample in the Reaction Chamber. ( 6 ) FDO Reagent DP (1.0 mL) Dispenses aliquot of STROMATOLYSER-FD(I) into the 4DIFF dilution sample in the Reaction Chamber. 10-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 458 SV33, and Master Valves MV19 through MV32. ( 1 4 ) Valve Unit No. 91 Assembly Controls the fluid flow. This assembly includes Solenoid Valves SV34 through SV45, and Master Valves MV33 through MV46. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-29...

  • Page 459: Right Interior

    Hard Disk Drive M2681SA Stores program, setting data, analyzed patient data, and QC data. ( 5 ) Floppy Disk Drive JU-256A025P Writes and reads data to and from a pre-formatted floppy disk. 10-30 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 460 PCB No. 9243 This is a mother board. ( 7 ) CPU Unit No. 12 This CPU board and extended memory board control the entire SF-3000 electric system. ( 8 ) I/O Board No. 5 Assy This I/O board has following interfaces and functions; a keyboard interface, printer...

  • Page 461: Front

    Make sure that the ball float is inside. ( 5 ) Power Switch Turns the Pneumatic Unit power ON and OFF. CAUTION: Do not turn it ON and OFF repeatedly in a short period of time. 10-32 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 462: Right Side

    ( 4 ) Pressure Outlet Nipple Pressure is supplied to the Main Unit through this nipple. Connects with the pressure inlet nipple on the air drier on the rear panel of the Main Unit. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-33...

  • Page 463: Top Interior

    (1) Compressor Pneumatic Unit – Right Interior Figure 10-29: ( 1 ) Compressor Generates both pressure and vacuum for the system. ( 2 ) Fan Exhausts heat generated in the Pneumatic Unit. 10-34 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 464: Top

    ( 6 ) Blood Mixer/Aspirator Inverts a sample ten times for mixing; then aspirates the blood via the Cap Piercing Unit. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-35...

  • Page 465: Top Interior

    Controls the solenoid valves, sensors and stepping motors in the Sampler Unit. NOTE: Parts information for ordering is given below. Part Code No. Part Description 943-2351-1 PCB No. 1233 943-2481-8 ROM 4SF31 Assy 10-36 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 466 Open to set a collection tube after mixing. ( 2 ) MCP Unit Cover Can be removed by pulling it straight up. WARNING! Do not remove this cover while the MCP unit is operating. Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-37...

  • Page 467: Reagent System

    Lyses red blood cells and releases hemoglobin for measurement by the SLS- Hemoglobin Method. Detergent: CELLCLEAN Strong alkaline detergent which removes lyse reagents, cellular residuals, and blood proteins remaining in the hydraulics. 10-38 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 468 • Product life unopened after manufacturing is 12-18 months. • Avoid direct sunlight during storage and use. • If reagent displays any signs of contamination, cloudiness, instability or color change, replace it. Sysmex SF-3000 Operator's Manual -- Added September 1995 10-39...
  • Page 469 CHAPTER 11 SYSTEM SETUP INTRODUCTION ............. 11-1 AUTO MANAGEMENT PROGRAMS........11-3 2.1 Auto Output Condition..........11-6 2.2 Auto Output Mode (Data Specification)....... 11-8 2.3 Auto Erase..............11-9 2.4 Auto Validation ............11-11 DATA CRITERIA............. 11-13 3.1 Mark Limits ............. 11-16 3.2 Critical Limits ............

  • Page 470: Chapter 11 System Setup

    SYSTEM SETUP INTRODUCTION System setup of the SF-3000 will be performed by the Sysmex Service Representative when the instrument is installed. However, the settings can be changed according to each laboratory's needs by using the Setting Programs explained in this chapter. This chapter consists of the following sections.
  • Page 471 SYSTEM SETUP General Set Up Explains setting of SF-3000 system status: • Date/Time Sets the date and time on the SF-3000 built-in clock. • Units Sets the analysis units to be used for the display screen and printouts. • Timer Mode Activates or deactivates the timer used to shut off the pneumatic power.

  • Page 472: Auto Management Programs

    • Once sample data has been output to an output device, it will not be output to the same device or be validated automatically. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-3...
  • Page 473 Ready Manual Mode Next No.1234567890123 Sysmex Sampler DP:1234567890123 XM HC GP DP SU • Settings • Output Output Auto Auto Return Cond. Mode Erase Valid. Figure 11-2: Auto Management Submenu Screen 11-4 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 474 Continue : To return to the previous screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-5...

  • Page 475: Auto Output Condition

    Press the appropriate key to select the condition. The condition selected will be backlit in light-blue. NOTE: The factory setting is "FIFO" for all devices. The available conditions are listed in Table 11-1. 11-6 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 476 : To return to Auto Output Condition setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-7...

  • Page 477: Auto Output Mode (Data Specification)

    Continue : To return to Output Mode setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. 11-8 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 478: Auto Erase

    : No data is automatically erased. Data will need to be erased manually when memory is full. FIFO : Erase the oldest data automatically. This is the factory setting. NOTE: The factory setting is "FIFO". Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-9...
  • Page 479 When the remaining memory falls below for one sample capacity the "Data File Full" appears, stopping the analysis. Erase unneeded data using the Stored Data program and set Auto Erase to "FIFO" if needed. 11-10 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 480: Auto Validation

    SYSTEM SETUP 2 . 4 Auto Validation SF-3000 Auto Output function outputs only data that is validated for reporting. Use this program to accept previously specified sample data to output to the peripheral devices. To set auto validation: Press [Auto Valid.] key in the Auto Management submenu.
  • Page 481 : To return to Auto Valid. setting screen so that setting can be continued. : To save the new settings. Program ends. Quit : To cancel the new settings. Program ends. 11-12 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 482: Data Criteria

    Sysmex Sampler DP:1234567890123 XM HC GP DP SU • Setting • Auto Data Flag General Print Config. Return Mgmt. Criteria System Set Up Set Value Figure 11-9: Settings Second Menu Screen Sysmex SF-3000 Operator's Manual -- Revised March 1996 11-13...
  • Page 483 Ready Manual Mode Next No.1234567890123 Sysmex Sampler DP:1234567890123 XM HC GP DP SU • Settings • Mark Critical Stop Stop Return Limits Limits Limits Cond. Figure 11-11: Data Criteria Submenu Screen 11-14 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 484 Continue : To return to the previous screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-15...

  • Page 485: Mark Limits

    [ keys to scroll up and down. • Pressing [ENTER] key after inputting the new value has the same effect as pressing the [→] key. 11-16 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 486 : To return to Mark Limits setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-17...

  • Page 487: Critical Limits

    [X10 / uL] QUIT Mark Critical Stop Stop Return Limits Limits Limits Cond. Figure 11-14: Critical Limits Setting Window NOTE: The settings illustrated on the screen above are the factory settings. 11-18 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 488 : To return to Critical Limits setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-19...

  • Page 489: Sampler Stop Limits

    [X10 / uL] QUIT Mark Critical Stop Stop Return Limits Limits Limits Cond. Figure 11-15: Stop Limits Setting Window NOTE: The settings illustrated on the screen above are the factory settings. 11-20 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 490 Press the appropriate key [Continue], [Set] or [Cancel]. Continue To return to Stop Limits setting screen so that setting can be continued. To save the new settings. Program ends. Cancel To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-21...

  • Page 491: Sampler Stop Condition

    3 . 4 Sampler Stop Condition Use this program to set the sampler (auto mode) analysis stop conditions. The SF-3000 will stop sampler analysis when an error occurs if the conditions for the error are enabled in the sampler stop conditions program.
  • Page 492 [Continue], [Set] or [Cancel]. Continue To return to Stop Conditions setting screen so that setting can be continued. To save the new settings. Program ends. Cancel To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-23...

  • Page 493: Flag System

    Sysmex Sampler DP:1234567890123 XM HC GP DP SU • Setting • Auto Data Flag General Print Config. Return Mgmt. Criteria System Set Up Set Value Figure 11-17: Settings Second Menu Screen 11-24 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 494 XM HC GP DP SU • Settings • Return Figure 11-19: Flag System Submenu Screen Press the desired keypad to execute the program. Proceed each program by referring to the following sections. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-25...
  • Page 495 Continue : To return to the previous screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. 11-26 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 496: Wbc

    2 . 5 0 [X10 /uL] Leukocytosis WBC# 1 8 . 0 0 [X10 /uL] • QUIT Return Figure 11-21: WBC Flag System Setting Window NOTE: The factory settings are given in Table 11-2. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-27...
  • Page 497 Continue : To return to WBC setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. 11-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 498: Rbc

    RDW-CV > 20.0 [%] Microcytosis MCV < 70.0 [fL] Macrocytosis MCV > 110.0 [fL] Hypochromia MCHC < 29.0 [g/dL] Anemia HGB < 10.0 [g/dL] / µ L] Erythrocytosis RBC > 6.50 [x 10 Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-29...

  • Page 499: Plt

    DP:1234567890123 XM HC GP DP SU ABNORMAL COMMENT ITEM VALUE Thrombocytopenia [X10 /uL] Thrombocytosis 6 0 0 [X10 /uL] ↑ ↓ ENTER QUIT Return Figure 11-23: PLT Flag System Setting Window 11-30 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 500 Continue : To return to PLT setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-31...

  • Page 501: Peripheral Configuration

    XM HC GP DP SU • Setting • Auto Data Flag General Print Config. Return Mgmt. Criteria System Set Up Set Value Figure 11-24: Settings Second Menu Screen Press [Config.] key in the second menu. 11-32 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 502 XM HC GP DP SU • Settings • Return Setting Setting Setting Figure 11-26: Configuration Submenu Screen Press the desired keypad to execute the program. Proceed each program by referring to the following sections. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-33...
  • Page 503 Continue : To return to the previous screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. 11-34 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 504: Hc Setting (Host Computer)

    : The transmission procedure protocol can be selected. (The factory setting is "Class A".) Interval : Time interval between data transmitted to the Host Computer can be selected. (The factory setting is "2" seconds.) Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-35...
  • Page 505 Continue : To return to HC setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. 11-36 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 506: Gp Setting (Graphic Printer)

    : Select this for the Graphic Printer model DeskJet 560C. Canon BJC : Select this for the Graphic Printer model Canon BJC 400J (for Japanese domesticmarket). Dot Imp. : Select this for the dot impact printer model OKI MICROLINE 320. Sysmex SF-3000 Operator's Manual -- Revised July 1996 11-37...

  • Page 507: Dp Setting (Data Printer)

    (√) is placed in the box to the right of selected item. Press the appropriate keypads to select the condition. There are two screens for DP settings. Press [More] key to alter the screens. 11-38 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 508 : Select one of the date print formats. (The factory setting is "mmddyy".) Delimiter of Date : Three different delimiters can be used between the figures for day, month and year. (The factory setting is "/".) Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-39...
  • Page 509 Continue : To return to DP setting screen so that setting can be continued. : To save the new settings. Program ends. Quit : To cancel the new settings. Program ends. 11-40 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 510: General Set Up

    SYSTEM SETUP GENERAL SET UP Use this program to set SF-3000 system status. To execute General Set Up programs: Press [Settings] key in the root menu. The Settings second menu will be displayed. NOTE: If the [Settings] keypad is not displayed on the LCD, press [More] key to change the displayed root menu.
  • Page 511 Password More Return Time Mode Range Format Figure 11-35: General Set Up Submenu Screen Press a desired keypad to execute a program. Proceed each program by referring to the following sections. 11-42 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 512 Continue : To return to the previous screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-43...

  • Page 513: Date/Time

    SYSTEM SETUP 6 . 1 Date/Time The SF-3000 has a built-in clock that is powered by an internal battery whenever the power is turned off or interrupted, so that the date and time need only be reset occasionally. To set date and time: Press [Date/Time] key in the General Set Up submenu.

  • Page 514: Units

    1 [ X 1 0 2 / u L ] 2 [ X 1 0 3 / u L ] 5 [ X 1 0 9 / L ] QUIT Date/ Timer Normal Date Units Password More Return Time Mode Range Format Figure 11-38: Units Setting Screen Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-45...
  • Page 515 Continue : To return to Units setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. 11-46 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 516: Password

    Continue : To return to Password setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised December 1995 11-47...

  • Page 517: Timer Mode

    : To return to Timer Mode setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. 11-48 Sysmex SF-3000 Operator's Manual -- Revised March 1996...

  • Page 518: Normal Range Display

    : To return to Normal Range setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-49...
  • Page 519 Normogram P L T R B C 40fL 250fL Figure 11-42: Normal Range Display 11-50 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 520: Date Format

    : To return to Date Format setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-51...

  • Page 521: Id Reader

    : Select "Used" or "Not Used" for test tube ID. (The factory setting is "Used".) Rack ID : Select "Used" or "Not Used" for rack ID. (The factory setting is "Used".) 11-52 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 522 : To return to ID Reader setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised September 1995 11-53...

  • Page 523: Blood Sensor

    If it is known beforehand that samples of low volume or weak blood (like dialyzed patient's blood) are to be analyzed, set this option to "Not Used" condition by deselecting it (deleting the " √ " mark). 11-54 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 524 : To return to Blood Sensor setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. Sysmex SF-3000 Operator's Manual -- Revised October 1995 11-55...

  • Page 525: Work List

    : To return to Work List setting screen so that setting can be continued. : To save the new settings. Program ends. Cancel : To cancel the new settings. Program ends. 11-56 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 526: Output Set Values

    Insert FD and Press OK key. Cancel Auto Data Flag Condition P r i n t Backup Config Return Manage. Criteria System Setting Settings Set Value Figure 11-47: FD Backup Setting Window Sysmex SF-3000 Operator's Manual -- Revised March 1996 11-57...
  • Page 527 Figure 11-49: FD Write Error Message Window Press [Cancel] to cancel processing and exit the program. NOTE: Only the output of the setting values is possible. To restore the setting values, contact your Sysmex service representative. 11-58 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 528 More More Sysmex Sampler Rack Hard Copy Error Log Tube Sample No. Start Mode/Next No. Manual Mode Capillary Mode Closed Mode (Refer to the following pages for the sub-programs of Root Menu.) Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 529 Return Previous Data More Next Data b8 Return Return A-5 GP PRINT N O T E : The program indicated with "P" A-6 DP PRINT needs the password entry. A-7 REPLACE REAGENT Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 530 Return RBC/PLT More b1 ID Reader Counters Return b2 Blood Sensor Worklist 1 Version Check b7 More 2 Format FD b8 Return More Print Set Value Return Backup Set Value Return Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 531 MENU TREE Calib. HGB/HCT Auto Setting Analyze Exclude Exe. Cal. Graphic Return HGB/HCT Manual Cal. History Return Shutdown Auto Rinse Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 532 10.2 Pneumatic Cable Connections..........B-30 10.3 Power Cord Connections..........B-30 11. POWER ON AND SYSTEM SETUP ........B-31 11.1 Power On..............B-31 11.2 System Setup.............B-31 11.3 System Check............B-32 Sysmex SF-3000 Operator's Manual -- Revised June 1996 Sysmex SF-3000 Operator's Manual -- Revised December 1995...

  • Page 533: Preinstallation

    • Graphic Printer or Line Printer (Optional) • Data Printer (Optional) Your Sysmex representative is responsible for unpacking, installing, and the initial set up of the SF-3000 system. The information in this section is given only for your completeness. 1 . 1 Unpacking Check List...
  • Page 534 - - - 348-9502-9 Washer Flat M4 (SUS) 348-9502-9 Washer Flat M4 (SUS) * These silicone tubes are used for the servicing purpose only. Sysmex SF-3000 Operator's Manual -- Revised June 1996 Sysmex SF-3000 Operator's Manual -- Revised March 2001...
  • Page 535 Washer Flat M4 (SUS) 368-0273-5 Rubber Cap No.33 Bar Code Reader Unit Carton (Option) Part No. Description Quantity 943-0501-8 BR-4 Assy 348-3812-1 Screw Binding M3X6 (SUS) Sysmex SF-3000 Operator's Manual -- Revised September 1998 Sysmex SF-3000 Operator's Manual -- Revised March 2001...

  • Page 536: Supplementary Parts Information

    INSTALLATION, Preinstallation 1 . 2 Supplementary Parts Information When the Sampler Unit and/or Bar Code Reader is not installed for the SF-3000, the dummy cover and some parts to cover the opening are available. Also when the Manual CP Unit is installed, some parts are necessary to install the dummy cover.

  • Page 537: Layout And Work Space

    The power cords are 1.8 m long and the drain tube is 10 m long in the maximum. The Pneumatic Unit, reagents and printers should also be installed in convenient location. Figure B-1: Dimensions of System Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 538: Removing Packing Material

    Open the front cover. WARNING! When working with the front cover open, make sure that the stop bar is in place. Stop Bar Figure B-3: Set Stop Bar Remove the screws fastening the front panel. Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 539 Connect the grounding wire (Wiring Cord No. 1698) to the front panel using one M4 screw together with M4 washer spring. See Figure B-5. (M4) Grounding Wire Sampler Dummy Cover Figure B-5: Install the Dummy Cover(s) Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 540: Main Unit Chassis

    • If you use the CP Sampler (option), do not seal the holes. • If you use the Manual CP Unit (option), seal the left side hole only. Attach the supplied sealing tape. Figure B-7: Main Unit Chassis Sysmex SF-3000 Operator's Manual -- Revised August 1998...

  • Page 541: Srv

    • Do not bend the manual aspiration pipette when pulling out the valve. 3 . 5 Rear Panel Remove the rubber caps protecting the reagent nipples. Remove the Remove the rubber caps. rubber caps. Figure B-9: Rear Panel B-10 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 542: Pneumatic Unit

    Remove the 2 screws fastening the compressor to the bottom plate of the Pneumatic Unit. Keep the screws in the parts box for future use. Figure B-10: Pneumatic Unit TRAY Install the supplied tray. Tray Figure B-11: Tray Sysmex SF-3000 Operator's Manual -- Revised September 1995 B-11...

  • Page 543: Bar Code Id Reader (Optional

    Pass the wire cord through the slit of ID Unit (see Figure B-13). Connect the wiring cord to the ID Unit. Pass the cord through the slit. Figure B-13: Connect the Cord B-12 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 544: Cp Sampler Unit (Optional

    Make sure that there is no gap between the rail and the bottom surface of the Main Unit Chassis. Sampler Front Panel Mounting Metal No.12 Figure B-14: Front Panel Figure B-15: Attach Sampler Mounting Metals Sysmex SF-3000 Operator's Manual -- Revised September 1995 B-13...

  • Page 545: Sampler Unit

    Mount the hand clipper using the attached screws (M3 x 25). Remove the mixer unit fixing plate. Screws (M3X25) Figure B-18: Remove the Fixing Plate B-14 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 546: Connection To Main Unit

    Reinstall the Main Unit front panel. Push the sampler unit all the way toward the Main Unit. Make sure that the Sampler Unit is fixed securely with the catchers. Front Panel Figure B-20: Install the Sampler Sysmex SF-3000 Operator's Manual -- Revised September 1995 B-15...
  • Page 547 Figure B-21. Whole Blood Aspiration Tube Figure B-21: Connect Whole Blood Aspiration Tube Install the sampler CP cover. Pass the whole blood aspiration tube through the guide in the cover. B-16 Sysmex SF-3000 Operator's Manual -- Revised August 1998...

  • Page 548: Manual Cp Unit (Optional

    KCP-4 dummy cover with the M4 screw. (M4) Figure B-22: Attach Grounding Wire Remove four fixing screws and SCREWS open the LCD Unit. Then PCB No.1234 will appear. SCREWS Figure B-23: Open LCD unit Sysmex SF-3000 Operator's Manual -- Revised August 1998 B-17...

  • Page 549: Manual Cp Unit

    Main Unit Chassis. See the figure below. Grounding wire from MCP dummy cover Grounding wire from MCP unit Figure B-25: Connect Grounding Wires Reinstall the front panel on the Main Unit. B-18 Sysmex SF-3000 Operator's Manual -- Revised August 1998...
  • Page 550 Figure B-26: Attach Sampler Mounting Metals Use a screwdriver to remove the dummy cover from the fluid connector. Insert the Manual CP unit halfway onto the mounting rail. Sysmex SF-3000 Operator's Manual -- Revised August 1998 B-19 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 551 MCP Unit is fastened securely with the catchers. Fasten the Manual CP Unit to the Main Unit Chassis with the screw binding (see Figure B-28). Screw Binding (M3 x 6) M3x6 Figure B-28: Install Manual CP Unit B-20 Sysmex SF-3000 Operator's Manual -- Revised May 1996...
  • Page 552 For use of SARSTEDT collection tube, please order and prepare Piercer SE-M SA (PM) (P/N 921-0776-2). (12) Close the MCP cover. (13) After installation completes, verify that blood aspiration is performed without any problem. Figure B-30: Close MCP Cover Sysmex SF-3000 Operator's Manual -- Revised August 1998 B-21...

  • Page 553: Tube Connection

    Air Drier Outlet Nipple Air Drier Inlet Nipple Screw Bindings M4 x 20 Vacuum Inlet Nipple Pressure Inlet Nipple Drain Inlet Nipple Pneumatic Unit VACUUM PRESSURE Figure B-31: Pneumatic Tube Connections B-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 554: Reagent Container Preparation

    Dispenser Kit No. 1 for STROMATOLYSER-FD (II). • Attach the Indication Mark "SLS" to the cap of the Dispenser Kit No. 1 for SULFOLYSER. Figure B-33: Attach the Label Sysmex SF-3000 Operator's Manual -- Revised September 1995 B-23...
  • Page 555 Keep the container cap in a safe place for future reuse. Figure B-34: Assemble Cubitainer Spout Kit No. 1 CAUTION: Verify that the Cubitainer Spout Kit No.1 stands vertically in the Cubitainer. B-24 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 556 Insert the Cubitainer Spout Kit into the container and screw down the cap. Keep the container cap in a safe place for future reuse. Figure B-36: Assemble Cubitainer Spout Kit CS-61 Sysmex SF-3000 Operator's Manual -- Revised September 1995 B-25...

  • Page 557: Reagent Lines

    • Store reagent between of 5 - 30˚C. (Do not let reagent freeze.) • Do not let dirt, dust, or germs get into the reagent after it has been opened. Reagent must be used within two months of opening. B-26 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 558: Waste Container

    Figure B-38: Waste Tube Connection CAUTION: Position the waste sewer or waste container lower than the instrument waste nipple. The length of the waste tube should not exceed ten meters (10 m). Sysmex SF-3000 Operator's Manual -- Revised September 1995 B-27...

  • Page 559: Battery Backup

    Connect the battery connector to the J11 of PCB No. 6358 as shown in Figure B-41. Battery PCB No. 6358 Figure B-41: Connect Battery Connector Install the PCB No. 6358. Install the right side panel, and secure it with two screws. B-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 560: Output Connections

    Pneumatic Unit Power Inlet Figure B-42: Cable and Power Cord Connections NOTE: • If a Data Printer is to be connected to the SF-3000, a separate "CABLE PRINTER C-11 (DP/GP/J-3100)"(P/N 265-7660-9) is required. • If a user-supplied graphic printer is to be connected to the SF- 3000, a "CABLE PRINTER C-11 (DP/GP/J-3100)"(P/N 265-...
  • Page 561 Power Cord Connections Connect the Main Unit power connector to the AC power outlet using the power cord provided in the supply part. B-30 Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised March 2001...

  • Page 562: Power On

    INSTALLATION 1 1 . POWER ON AND SYSTEM SETUP The following procedures should be done by your Sysmex service representative at the time of installation. The outline of each procedures are described below for your reference. 1 1 . 1...

  • Page 563: System Check

    Run the WBC Flow Cell air bubble removal sequence by pressing the [WBC Flow Cell Air Bubble Removal]. Refer to Chapter 5, Section 7.5: Execute WBC Flow Cell Air Bubbles Removal Sequence. B-32 Sysmex SF-3000 Operator's Manual -- Revised September 1995 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 564 If it falls outside the range, adjust the PLT sensitivity. • Accuracy Analyze the SF-CHECK five times in the Manual mode. Verify that the average of the five results falls within 2SD of the assayed target value. Sysmex SF-3000 Operator's Manual -- Revised September 1995 B-33...
  • Page 565 If not found within the specification, obtain and set the calibration value. • ID Reader NOTE: When adjusting the ID Bar-Code Reader, contact your Sysmex service representative for his/her instruction and assistance. Pull out the Sampler Unit by approx. 10 cm, and remove the Main Unit Front Panel-B Assy.
  • Page 566 When the adjustment is complete, pull out the Sampler Unit by approx. 10 cm, and refit the Front Panel-B Assy. Refit the Sampler Unit. CAUTION: When refit the Sampler Unit, take care not to pinch the tubing. Sysmex SF-3000 Operator's Manual -- Revised September 1995 B-35...
  • Page 567 3.5 Dimensions of Bar Code Label ........C-20 3.6 C h e c k - D i g i t .............. C-20 3.7 Applicable Characters ..........C-28 3.8 Effective Bar Code Length ..........C-28 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 568: Serial Interface For Host Computer

    A serial interface is available on the Main Unit rear panel for connecting to a host computer. The bit serial voltage type, which conforms to the RS-232C interface, is used for input and output to and from the SF-3000. 1 . 1 Connection Connect an EIA RS-232C V.24 standard 9-pin D-SUB, female (body = female and pins...

  • Page 569: Signal Level

    Table C-3: RS-232C Signal Level 1 . 6 Interface Circuit Output Circuit 300K EMI Filter MC145407 Driver Figure C-1: Interface Output Circuit Input Circuit EMI Filter 5.4K MC145407 Receiver Figure C-2: Interface Input Circuit Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 570: Software

    Auto Out -- The SF-3000 automatically sends out analysis Analysis data output data after each analysis has been completed. Stored Data -- The SF-3000 sends out data from the stored data in a batch. Inquiry (output) and settings from When SF-3000 inquires order information by the Rack No.
  • Page 571 If necessary the host computer also checks the contents of the text (or block). The SF-3000 waits for the response ACK or NAK from the host computer in addition to two control signals (CTS and DSR) and transmits the next sample data upon receiving ACK from the host computer.
  • Page 572 If the host computer receives ACK from the SF-3000, the host computer completes the communication of order information for one sample. If NAK is received from the SF-3000, the host computer re- sends the order information text to the SF-3000.
  • Page 573 Rack No. and Tube Position No., and the system analyzes the sample according to the order by position information (Rack No., Tube Pos. No.). There is no time limit for the SF-3000 to receive the order information after transmitting the inquiry to the host computer.

  • Page 574: Text Format

    TECHNICAL INFORMATION TEXT FORMAT The SF-3000 instrument transmits (1) Patient Sample Data, (2) Quality Control (QC) Data and (3) Inquiry Data. These data are distinguished by the Text Distinction Code I and Sample Distinction Code. (Text length is different among the transmittable data.) Text Distinction Code I is "D"...
  • Page 575 PDW (fL) (XXX.XF) MPV (fL) (XXX.XF) P-LCR (%) (XXX.XF) Reserved 1 Reserved 2 Reserved 3 Reserved 4 Reserved 5 Reserved 6 Reserved 7 Reserved 8 Reserved 9 Reserved 10 Reserved 11 Reserved 12 Sysmex SF-3000 Operator's Manual -- Revised March 2001...
  • Page 576 Order Information indicates if the order information exists at the time of sample analysis. "0": The order information does not exist. "1": The order information exists. Reserved Flag -- (Not used and "0" is transmitted.) C-10 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 577 0 (zero), thus "*0000" will be sent for the analysis result. If the sample was not analyzed according to the order information, four or five blank spaces will be sent " ". Reserve 1 through 12 -- (Not used) Sysmex SF-3000 Operator's Manual -- Revised September 1995 C-11...

  • Page 578: Quality Control Data Format

    "B": X or L-J Control File No. 11 "C": X or L-J Control File No. 12 "M": X M Control File The other parameters are the same as those for the Patient Sample Data Format. C-12 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 579 MONO# (x 10 /µL) (XXX.XX) EO# (x 10 /µL) (XXX.XX) BASO# (x 10 /µL) (XXX.XX) Reserved 1 Reserved 2 GRAN-X (XXX.X) GRAN-Y (XXX.X) WBC/BA-X (XXX.X) WBC/BA-Y (XXX.X) GRAN-Y (W) (XXX.X) Reserved 3 (XXX.X) Sysmex SF-3000 Operator's Manual -- Revised March 2001 C-13...

  • Page 580: Inquiry Data Format

    This becomes valid only when the batch inquiry is performed. Zero suppression is not performed. INQUIRY DATA FORMAT -- 21 Bytes Parameter No. of Chars. Example Text Distinction Code I "R" Inquiry Mode Sample ID No. Rack No. Tube Position No. C-14 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 581: Order Information Data Format

    Data Format when the batch inquiry is performed using the Rack No. and Tube Position No. Inquiry Mode "1": (Not Used) "2": Batch Inquiry, using the Rack No. and Tube Position No. Order Information for each Parameter "0": Do not analyze "1": Analyze Sysmex SF-3000 Operator's Manual -- Revised September 1995 C-15...
  • Page 582 Reserved 6 Reserved 7 MCHC LYMPH% MONO% NEUT% BASO% LYMPH# MONO# NEUT# BASO# RDW-CV RDW-SD P-LCR Reserved 8 Reserved 9 Reserved 10 Reserved 11 Reserved 12 Reserved 13 Reserved 14 Reserved 15 C-16 Sysmex SF-3000 Operator's Manual -- Revised September 1995...

  • Page 583: Id Bar Code

    TECHNICAL INFORMATION ID BAR CODE 3 . 1 Applicable Bar Codes The types of bar codes acceptable to the SF-3000 and the relation of the check-digit to each bar-code type are as follows: Sample ID No. Type of Bar-Code Check-Digit No.

  • Page 584: Dimensions Of Elements

    Rs: the reflectivity of the space (background) Rb: the reflectivity of the black inked bar The measuring method conforms to the JIS (Japanese Industrial Standards) B9550, "5.3 Optical Characteristics of Bar Code Symbols". C-18 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 585 When a bar element is magnified, the following may be observed. The variation coefficient (S) in the width of a bar should be less than or equal to 20%. Max-Min S(%) = x 100 B a r Magnified Bar Figure C-6: Sysmex SF-3000 Operator's Manual -- Revised September 1995 C-19...

  • Page 586: Dimensions Of Bar Code Label

    Add all odd digits. Multiply the sum by 3. Add all even digits. Add the result of (2) and result of (3) above. C-20 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 587 However, in Example No. 2, the sum of the total number of the data digits and the check-digit gives odd number 7 in this case. Therefore, "0" is prepended to the most significant digit (left most digit) and check-digit is appended to the data, as 05243624. Sysmex SF-3000 Operator's Manual -- Revised September 1995 C-21...
  • Page 588 15-2345-67894. When the ID Reader reads this bar code label, the instrument computes the check- digit(s) and recognizes the read as a valid read if the remainder is 0 or 1. C-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 589 Divide the sum by 11 and get the remainder. Then subtract the remainder from 11. The result will be the check-digit. 323/11 = 29; remainder = 4, 11-4 = 7, Hence the check-digit is 7. Sysmex SF-3000 Operator's Manual -- Revised September 1995 C-23...
  • Page 590 D9981474D. When the ID Reader reads this bar code label, the instrument computes the check- digit and recognizes the read as a valid read if the remainder is 0. C-24 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 591 In this example, the letter "J" has the value of 19 which is equal to the remainder. Therefore "J" is the check-character. This check-character is appended to the ID number, after the least significant digit. The bar-code label is now "258-416J". Sysmex SF-3000 Operator's Manual -- Revised September 1995 C-25...
  • Page 592 The sum of the products is 829. This sum is divided by 103 as; 829/103 = 8 and remainder is 5. The corresponding character for the value 5 is %. Hence the check-digit is %. C-26 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 593 FNC2 FNC2 SHIFT SHIFT CODE C CODE C CODE B FNC4 CODE B FNC4 CODE A CODE A FNC1 FNC1 FNC1 START (Code A) START (Code B) START (Code C) STOP Sysmex SF-3000 Operator's Manual -- Revised September 1995 C-27...

  • Page 594: Applicable Characters

    CODABAR (NW-7) and CODE-39 may use the other characters such as alphabets. The SF-3000 ID system does not recognize them. The ID number allows up to thirteen digits. The application of hyphens in an ID number should adhere to the following rules: Hyphens must be placed between other characters.
  • Page 595 Battery Backup, Procedures B-28 <Analysis Error>, Troubleshooting 8-38 Baud Rate Analysis Mode Display 1-11 Serial Interface Analysis Parameters 11-35 Analysis Process, Status Display 8-92 BBM Unstorageable, Troubleshooting 8-45 Bellows Unit 9-5, 10-24 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 596 Cubitainer Spout Kit CS-61, Preparation B-25 Sampler Cubitainer Spout Kit No. 1, Preparation B-24 Waste Fluid Current Data, Stored Data Check List Delete 4-59 Maintenance Output 4-51 Unpacking Current Data, Work List, Delete 3-12 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 597 Down Load, Work List Function, Error Messages Fuses, Replace 5-65 DP Indicator 1-11 DP No. Display 1-10 DP Print, Test 8-80 DP Settings 11-38 Dummy Covers, Order Information Dutch SI Setting 11-46 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 598 Layout and Work Space Host Errors, Troubleshooting 8-49 LCD Screen 1-10 Host Format Contrast Adjustment Humidity, Environmental 1-20 Test 8-78 Left Interior, Main Unit 10-26 Left Side, Main Unit 10-24 Limit# or Limit%, QC, Select Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 599 Operator's Manual Organization Delete 4-60 Optical Requirements, Bar Code Label C-18 Output 4-52 Optical Alignment Check, Installation B-33 Marked Data, Work List, Delete 3-11 Maximum Allowable Difference between Manual and Auto Mode 1-24 Sysmex SF-3000 Operator's Manual -- Revised July 1996...
  • Page 600 Abnormal Limits, Set 11-30 Auto Mode 2-12 Criteria Closed Mode 2-13 List Error Messages 6-43 PLT Sampling Error, Troubleshooting 8-40 Manual CP Mode 2-17 Pneumatic Cable Connection B-30 Manual Mode 2-7, 6-41 Sysmex SF-3000 Operator's Manual -- Revised December 1995...
  • Page 601 RBC Detector Aperture, Clean 5-38 Relocation Information 1-19 RBC Detector Temp High, Remove Fluid from PU Trap Chamber Troubleshooting 8-11 RBC Detector Temp Low, Troubleshooting 8-11 RBC Fast To Start, Troubleshooting 8-37 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 602 Select Limit# or Limit%, QC [ S ] Select X or L-J Control Safety Select X /L-J QC Parameters Sample Data Format Select X M QC Parameters Sample Flow Block Diagram 10-4 viii Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 603 1-20 System Setup at Installation B-31 Specification System Specification 1-21 Bar Code Label C-17 System Status Area 1-10 Reagent 10-39 System 1-21 Clean 5-21 Location 10-25 Remove Packing Material B-10 Test 8-62 Sysmex SF-3000 Operator's Manual -- Revised March 1996...
  • Page 604 Tube Sensor Error, Troubleshooting 8-33 WBC Detector Temp Low, Turning On the Power Troubleshooting 8-13 Types of QC Method WBC Detector Thermal Sensor Discon., Troubleshooting 8-14 WBC Flow Cell 10-2 Air Bubbles Removal Sequence 5-41 Sysmex SF-3000 Operator's Manual -- Revised September 1995...
  • Page 605 < X Limit Error>, Troubleshooting 8-55 X or L-J Control, Select X M Control 6-48 Indicator 1-11 Limitations of 6-50 Start/Stop 6-10 < X M Limit Error>, Troubleshooting 8-55 [ Y ] Yearly Maintenance 5-26 Sysmex SF-3000 Operator's Manual -- Revised July 1996...

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