Create A Background Plate; Create A Background Array Card (Quantstudio ™ 7 Flex System Only) - Applied Biosystems QuantStudio 6 Flex User Manual

F
Appendix F Calibration consumable preparation
Create a background plate or array card
Create a
background plate
Create a
background array
card
(QuantStudio 7
™
Flex System only)
152
IMPORTANT! Wear powder-free gloves while creating the background plate.
1. Remove a MicroAmp
it on a clean, dry surface.
2. Aliquot 20 µL deionized water to each well of the reaction plate.
3. Seal the plate using an optical adhesive cover or optical flat caps.
4. Use the plate for background calibration as you would a background plate from
the spectral calibration kit.
1. Remove an array card from its box and place it on a clean, dry surface.
2. Using a permanent marker, write
"Background" on the side of the
empty card.
3. Pipet 100 µL of deionized water into
each of the eight reservoirs in the
card:
a. Place the array card on a lab
bench, with the foil side down.
b. Load 100 µL of the solution into a pipette.
c. Hold the pipette in an angled position (∼45°) and place the tip into the fill
port.
There is a fill port on the left arm of each fill reservoir – the larger of the two
holes.
Fill port
1
Vent port
2
QuantStudio 6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
™
™ 96/384-Well Optical Reaction Plate from its box and place
1 2
Mark
here