Appendix - Chromeleon Example Program - Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual

Uhplc+ focused, automated fraction collector
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10 Appendix - Chromeleon Example Program

;Example program for fraction collection with the AFC-3000
Automated Fraction Collector and the following instruments:
HPG-3x00, WPS-3000SL, VWD-3x00
TempCtrl =
Temperature.Nominal =
Temperature.LowerLimit =
Temperature.UpperLimit =
ReadyTempDelta =
Pressure.LowerLimit =
Pressure.UpperLimit =
MaximumFlowRampDown =
MaximumFlowRampUp =
%A.Equate =
%B.Equate =
DrawSpeed =
DrawDelay =
DispSpeed =
DispenseDelay =
WasteSpeed =
SampleHeight =
InjectWash =
LoopWashFactor =
PunctureOffset =
Sampler.PumpDevice =
InjectMode =
SyncWithPump =
Pump_Pressure.Step =
Pump_Pressure.Average =
Data_Collection_Rate =
TimeConstant =
AFC.Valve =
;Close the diverter valve prior to a run to avoid
accidental spillages
AFC.CollectMode =
;Define how the drop former travels over the collection
vessels.
;Do only define once and do not change during a run or
a sequence
AFC.MovementMode =
;Define how the diverter valve behaves between tube
changes.
AFC.TubePosition =
FractionCollection.TubePosition
;Synchronize the fraction collector position with
Operating Instructions
UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
On
20.0 [°C]
4.0 [°C]
45.0 [°C]
None
0 [bar]
150 [bar]
30.000 [ml/min²]
30.000 [ml/min²]
"%A"
"%B"
20.000 [µl/s]
3000 [ms]
2.000 [µl/s]
2000 [ms]
4.000 [µl/s]
1.000 [mm]
NoWash
0.000
0.0 [mm]
"Pump"
Normal
Off
Auto
On
25.0 [Hz]
0.06 [s]
Drain
SawVertical
Interrupt
Page 77

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