Thermo Scientific Dionex UltiMate 3000 Series Operating Instructions Manual page 10

UltiMate 3000 Series:
AFC-3000 Automated Fraction Collector
• Hazardous substances
Many organic solvents, mobile phases and samples are harmful to health. Be sure that
you know the toxic and infectious properties of all substances that you are using. You
may not know the toxic or infectious properties of many substances that you are using. If
you have any doubt about a substance, treat it as if it contains a potentially harmful
substance. For advice on the proper handling of a particular substance, refer to the Safety
Data Sheet (SDS) of the manufacturer. Observe the guidelines of Good Laboratory
Practice (GLP).
Dispose of waste substance in an environmentally safe manner that is consistent with all
local regulations. Do not allow flammable, toxic, and/or infectious substances to
accumulate. Follow a regulated, approved waste disposal program. Never dispose of
flammable, toxic, and/or infectious substances through the municipal sewage system.
Hazardous gases
•
Install the HPLC system in a well-ventilated laboratory. If the mobile phase or sample
includes volatile or flammable solvents, do not allow them to enter the workspace. If the
mobile phase or sample includes volatile or flammable solvents, avoid open flames and
sparks.
• Electrostatic discharge
Discharge of electrostatic energy may lead to sparking and can constitute a fire hazard.
This effect is particularly pronounced in insulating capillaries and with non-conductive
solvents (for example, pure acetonitrile).
Take appropriate measures to prevent the generation of static electricity near the HPLC
system. For example, make sure that the air humidity level in the laboratory is
sufficiently high and provide proper ventilation, wear anti-static clothing or shoes,
prevent accumulation of air bubbles in waste lines, and use grounded waste containers.
Use only non-conductive capillaries to direct solvents into the waste container. With
electrically conductive capillaries, make sure that they are properly grounded.
Operation with Normal-Phase Eluents:
Do not perform normal-phase applications without a proper grounding protection for the
drop former. Non-conductive, non-polar normal-phase eluents, for example hexane, can
lead to a build-up of electrostatic charges on an ungrounded drop former. The
electrostatic discharge can create a fire and/or explosion hazard.
Note that a grounded drop former (part no 6702.0400) is available for normal-phase (NP)
applications. Use normal-phase eluents with the AFC-3000 Automated Fraction
Collector only after it has been modified with this grounded drop former. Operating the
device without the grounded drop former can result in a fire and/or explosion
hazard!
• Self-ignition of solvents
Do not use solvents for which the self-ignition temperature is below 150 °C. In case of
leakage, these solvents may self-ignite on a hot surface.
Page 4 Operating Instructions