Mode To Mode Calibration Overview; Introduction; Auto-Cal Mode To Mode Calibration; Manual Mode To Mode Calibration - Abbott CELL-DYN 3000 Operator's Manual

Chapter 6

Mode To Mode Calibration Overview

Introduction

Auto-Cal Mode to Mode Calibration

Manual Mode to Mode Calibration

CELL-DYN® 3000 System Operator's Manual
9140240E — May 1995
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The Open mode of the CELL-DYN 3000 is calibrated with either a
commercial calibrator or fresh whole blood samples. The Closed mode is
calibrated to match the Open mode using 10 different, fresh, normal
whole blood samples. Samples used for Mode to Mode calibration
should meet the requirements outlined in the
section of this chapter.
Single replications of 10 samples can be used because calibration
differences between modes are usually very small. Samples may be run
more than once if desired but samples should be run the same number of
times in each mode. Calibration of the Closed mode with fewer than 10
samples is not recommended because it may introduce a bias between the
modes.
Worksheets are provided at the end of each procedure to assist in
determining which parameters require calibration. For manual
calibration, worksheets may be used to assist in making the necessary
calculations. These worksheets may be duplicated as needed.
After the Open mode has been calibrated or Open mode calibration is
confirmed, each sample is run one time in the Open mode. For ease of
identification, samples should be run as patient samples.
The Open mode values for each sample are entered as the reference
values in the Auto-Cal program for the Closed mode. The samples are
then run in the Closed mode. The factor percent difference computed by
the Auto-Cal program is used to determine which Closed mode
parameters require calibration.
After the Open mode has been calibrated or Open mode calibration is
confirmed, the samples are run into quality control files in the Open and
Closed modes.
The automatically calculated parameter means from each mode are used
to manually compute the calibration bias and the new calibration factors.
The new calibration factors are then entered manually from the
FACTOR Screen in the
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Whole Blood Calibration
CALIBRATION MENU.
Calibration
ENTER
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