Sheath Reagent; Detection With The Optical Bench - Abbott CELL-DYN 3000 Operator's Manual

Principles of Operation

Sheath Reagent

Detection with the Optical Bench

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Focused
Laser Beam
°
0 Scatter
°
10 Scatter
Figure 3.2: WBC Light Scatter
The Sheath reagent is an integral part of the WBC analysis. White blood
cells diluted in the Sheath reagent maintain cellular integrity close to
their native state. The structure of the basophils changes slightly due to
the water soluble nature of the basophilic granules.
The RBCs are also altered by the Sheath reagent. The RBCs are ruptured
by hypo-osmotic shock. The RBC ghosts have the same refractive index
as the Sheath reagent, thereby rendering them invisible to the laser.
The optical bench assembly contains the components that make up the
flow cytometer. It is depicted in
optical bench is to detect the light scattered by the cells as they pass
through the flow cell. The detection process is discussed in this section.
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Various Angles
of Scattered Light
Figure 3.3. The main purpose of the
CELL-DYN® 3000 System Operator's Manual
9140240E — May 1995
Chapter 3
°
90 Scatter
°
90 D Scatter