Preparing The Lc Modules - Agilent Technologies 6475 User Manual

4 Basic Operation

Preparing the LC Modules

Preparing the LC Modules

Read and follow the instructions in the online Help for each of the tasks in the
checklist described on the following pages. While you condition or equilibrate the
column, you can tune the Triple Quad LC/MS.
1 Switch LC stream to Waste.
2 Purge the LC pump. Follow the directions for purging the pump in the User
3 Set up to condition or equilibrate the column.
Agilent 6475 Triple Quad LC/MS System User Guide
When you are not acquiring data, you switch the direction of the LC stream
away from the MS ion source and to waste.
If you have the LC connected to a VWD or DAD, you can still monitor the
fluctuations of the VWD or DAD real-time chromatogram before a run.
a Right-click the QQQ device in the Instrument Status window.
b Select LC > Waste.
Guide for your pump.
a Type LC parameters, and click Apply in the toolbar to download them to
the LC.
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