Multiple-Level Calibration Sequences; Re-Calibrating With The Same Group Of Standards - Agilent Technologies InfinityLab LC Series User Manual

4 Running Multiple-Vial Analyses

Multiple-Level Calibration Sequences

Multiple-Level Calibration Sequences

The following sections describe how to set up calibration sequences for analyses
which use multiple- level calibration.

Re-calibrating With the Same Group of Standards

There are three calibration standards of different concentrations (C1, C2, C3) and
15 samples (S). The standards and samples are analyzed using the same
method.
The analysis requires that:
•
•
•
•
•
The example assumes that the sequence table is empty. If the sequence table is
not empty, use the Delete button to delete all lines or use File - New.
1 Select the Sequence screen.
2 Move to line 1 and press Insert.
3 Select Method and then move to METH1 and press Load.
4 Move to the End of sequence list, select the Wizard and enter:
Vial Range
#Inj.
5 Select Calibration to display the Calibration Settings screen.
G4208A User's Guide
each sample is analyzed twice,
the calibration standards are analyzed twice before the samples and re-
analyzed twice after every 5 samples,
C1 C2 C3 S10-S14 C1 C2 C3 S15-S19 C1 C2 C3 S20-S24 C1 C2 C3
the calibration standards are located in positions 90 – 92 of the autosampler
tray,
the 15 sample vials are located in positions 10 – 24 of the autosampler tray,
and
the samples and standards are analyzed using a method called METH1.
10 – 24
2
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