User Manuals: abi 7500 Real-Time PCR System

Manuals and User Guides for abi 7500 Real-Time PCR System. We have 1 abi 7500 Real-Time PCR System manual available for free PDF download: Getting Started Manual

abi 7500 Getting Started Manual (260 pages)

Real-Time PCR System

Brand: abi | Category: Laboratory Equipment | Size: 16 MB

Table of Contents
5
How to Use this Guide
9
Preface
9
How to Obtain more Information
11
How to Obtain Support
13
Safety Conventions Used in this Document
14
Symbols on Instruments
15
Safety Labels on Instruments
16
General Instrument Safety
18
Chemical Safety
19
Chemical Waste Safety
21
Electrical Safety
22
Physical Hazard Safety
22
Biological Hazard Safety
23
Workstation Safety
23
Safety and Electromagnetic Compatibility (EMC) Standards
24
Chapter 1 Get Started

25
Chapter 2 Design the Relative Standard Curve Experiment

44
- Chapter Overview
  44
- Create a New Experiment
  45
- Define the Experiment Properties
  46
- Define the Methods and Materials
  48
- Set up the Targets
  50
- Set up the Standards
  53
- Set up the Samples
  55
- Set up the Relative Quantitation Settings
  58
- Set up the Run Method
  59
- Review the Reaction Setup
  60
- Order Materials for the Experiment
  67
- Finish the Design Wizard
  70
- Prepare the Relative Standard Curve Reactions
  73
Chapter 3 Prepare the Relative Standard Curve Reactions

74
Chapter 4 Run the Relative Standard Curve Experiment

90
- Chapter Overview
  90
- Prepare for the Run
  91
- Enable the Notification Settings (Optional)
  93
- Start the Run
  95
- Monitor the Run
  96
- Unload the Instrument
  98
- Analyze the Relative Standard Curve Experiment
  99
Chapter 5 Analyze the Relative Standard Curve Experiment

100
- Chapter Overview
  100
- Section 5.1: Review Results
  101
Section 5.1 Review Results

101
- Analyze the Experiment
  102
- View the Standard Curve
  106
- View the Amplification Plot
  109
- View the Gene Expression Plot and Well Table
  117
- Publish the Data
  121
- Section 5.2: Troubleshoot (if Needed)
  123
Section 5.2 Troubleshoot (if Needed)

123
- View the Analysis Settings
  124
- View the QC Summary
  127
- Omit Wells from the Analysis
  129
- View the Multicomponent Plot
  131
- View the Raw Data Plot
  133
Chapter 6 Design the Comparative C Experiment

135
- Chapter Overview
  136
- Create a New Experiment
  137
- Define the Experiment Properties
  138
- Define the Methods and Materials
  140
- Set up the Targets
  143
- Set up the Samples
  145
- Set up the Relative Quantitation Settings
  148
- Set up the Run Method
  149
- Review the Reaction Setup
  151
- Order Materials for the Experiment
  155
- Finish the Design Wizard
  158
Chapter 7 Prepare the Comparative C

161
- Chapter Overview
  162
- T Reactions
  162
- Prepare the Template
  163
- Prepare the Sample Dilutions
  166
- Prepare the Reaction MIX
  168
- Prepare the Reaction Plate
  170
Chapter 8 Run the Comparative C Experiment

175
- Chapter Overview
  176
- Prepare for the Run
  177
- Run the Experiment
  178
Chapter 9 Analyze the Comparative C Experiment

179
- Chapter Overview
  180
- Section 9.1: Review Results
  181
Section 9.1 Review Results

181
- Analyze the Experiment
  182
- View the Gene Expression Plot and Well Table
  186
- View the Amplification Plot
  190
- Publish the Data
  198
- Section 9.2: Troubleshoot (if Needed)
  199
Section 9.2 Troubleshoot (if Needed)

199
- View the Analysis Settings
  200
- View the QC Summary
  203
- Omit Wells from the Analysis
  205
- View the Multicomponent Plot
  206
- View the Raw Data Plot
  209
- Section 9.3: Perform a Study of Multiple Experiments
  211
Section 9.3 Perform a Study of Multiple Experiments

211
- Section Overview
  212
- Design a Study
  213
- Analyze the Study
  219
- Publish the Data
  226
Appendix A Alternate Experiment Workflows

227
- Advanced Setup Workflow
  228
- Quickstart Workflow
  230
- Template Workflow
  232
- Export/Import Workflow
  234
- Bibliography
  237
- Glossary
  239
- Index
  253

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User Manuals: abi 7500 Real-Time PCR System

abi 7500 Getting Started Manual (260 pages)

Table of Contents

Chapter 1 Get Started

Chapter 2 Design the Relative Standard Curve Experiment

Chapter 3 Prepare the Relative Standard Curve Reactions

Chapter 4 Run the Relative Standard Curve Experiment

Chapter 5 Analyze the Relative Standard Curve Experiment

Section 5.1 Review Results

Section 5.2 Troubleshoot (if Needed)

Chapter 6 Design the Comparative C Experiment

Chapter 7 Prepare the Comparative C

Chapter 8 Run the Comparative C Experiment

Chapter 9 Analyze the Comparative C Experiment

Section 9.1 Review Results

Section 9.2 Troubleshoot (if Needed)

Section 9.3 Perform a Study of Multiple Experiments

Appendix A Alternate Experiment Workflows

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