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abi 7500 manual available for free PDF download: Getting Started Manual
abi 7500 Getting Started Manual (260 pages)
Real-Time PCR System
Brand:
abi
| Category:
Laboratory Equipment
| Size: 16.28 MB
Table of Contents
Table of Contents
5
How to Use this Guide
9
Preface
9
How to Obtain more Information
11
How to Obtain Support
13
Safety Conventions Used in this Document
14
Symbols on Instruments
15
Safety Labels on Instruments
16
General Instrument Safety
18
Chemical Safety
19
Chemical Waste Safety
21
Electrical Safety
22
Physical Hazard Safety
22
Biological Hazard Safety
23
Workstation Safety
23
Safety and Electromagnetic Compatibility (EMC) Standards
24
Chapter 1 Get Started
25
Get Started
25
About the 7500/7500 Fast System
26
Supported Consumables
28
About Relative Standard Curve and Comparative C Experiments
31
About Relative Standard Curve and Comparative C
31
How to Use this Guide
36
About the Example Experiments
38
Example Experiment Workflow
41
Design the Relative Standard Curve Experiment
43
Chapter 2 Design the Relative Standard Curve Experiment
44
Chapter Overview
44
Create a New Experiment
45
Define the Experiment Properties
46
Define the Methods and Materials
48
Set up the Targets
50
Set up the Standards
53
Set up the Samples
55
Set up the Relative Quantitation Settings
58
Set up the Run Method
59
Review the Reaction Setup
60
Order Materials for the Experiment
67
Finish the Design Wizard
70
Prepare the Relative Standard Curve Reactions
73
Chapter 3 Prepare the Relative Standard Curve Reactions
74
Chapter Overview
74
Prepare the Template
75
Prepare the Sample Dilutions
77
Prepare the Standard Dilution Series
79
Prepare the Reaction MIX
82
Prepare the Reaction Plate
84
Run the Relative Standard Curve Experiment
89
Chapter 4 Run the Relative Standard Curve Experiment
90
Chapter Overview
90
Prepare for the Run
91
Enable the Notification Settings (Optional)
93
Start the Run
95
Monitor the Run
96
Unload the Instrument
98
Analyze the Relative Standard Curve Experiment
99
Chapter 5 Analyze the Relative Standard Curve Experiment
100
Chapter Overview
100
Section 5.1: Review Results
101
Section 5.1 Review Results
101
Analyze the Experiment
102
View the Standard Curve
106
View the Amplification Plot
109
View the Gene Expression Plot and Well Table
117
Publish the Data
121
Section 5.2: Troubleshoot (if Needed)
123
Section 5.2 Troubleshoot (if Needed)
123
View the Analysis Settings
124
View the QC Summary
127
Omit Wells from the Analysis
129
View the Multicomponent Plot
131
View the Raw Data Plot
133
Chapter 6 Design the Comparative C Experiment
135
Chapter Overview
136
Create a New Experiment
137
Define the Experiment Properties
138
Define the Methods and Materials
140
Set up the Targets
143
Set up the Samples
145
Set up the Relative Quantitation Settings
148
Set up the Run Method
149
Review the Reaction Setup
151
Order Materials for the Experiment
155
Finish the Design Wizard
158
Chapter 7 Prepare the Comparative C
161
Chapter Overview
162
T Reactions
162
Prepare the Template
163
Prepare the Sample Dilutions
166
Prepare the Reaction MIX
168
Prepare the Reaction Plate
170
Chapter 8 Run the Comparative C Experiment
175
Chapter Overview
176
Prepare for the Run
177
Run the Experiment
178
Chapter 9 Analyze the Comparative C Experiment
179
Chapter Overview
180
Section 9.1: Review Results
181
Section 9.1 Review Results
181
Analyze the Experiment
182
View the Gene Expression Plot and Well Table
186
View the Amplification Plot
190
Publish the Data
198
Section 9.2: Troubleshoot (if Needed)
199
Section 9.2 Troubleshoot (if Needed)
199
View the Analysis Settings
200
View the QC Summary
203
Omit Wells from the Analysis
205
View the Multicomponent Plot
206
View the Raw Data Plot
209
Section 9.3: Perform a Study of Multiple Experiments
211
Section 9.3 Perform a Study of Multiple Experiments
211
Section Overview
212
Design a Study
213
Analyze the Study
219
Publish the Data
226
Appendix A Alternate Experiment Workflows
227
Advanced Setup Workflow
228
Quickstart Workflow
230
Template Workflow
232
Export/Import Workflow
234
Bibliography
237
Glossary
239
Index
253
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