Set Up The Run Method - abi 7500 Getting Started Manual

Set Up the Run Method

In the Run Method screen, review the reaction volume and the thermal profile for the
default run method. If needed, you can edit the default run method or replace it with one
from the Run Method library.
About the
In the relative standard curve example experiment, the default run method is used
Example without edits.
Experiment
Review the Run 1. In the Run Method screen, select either the Graphical View tab (default) or
Method Screen
2. Make sure the Reaction Volume Per Well field displays 50 µL.
3. Make sure the thermal profile displays the holding and cycling stages shown below.
4. Click Next.
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Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Chapter 2 Design the Relative Standard Curve Experiment
Tabular View tab.
If necessary, click a temperature or time setting, then change it.

Set Up the Run Method

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