Set Up The Samples - abi 7500 Getting Started Manual

For More For more information on:
Information
• Completing the Standards screen – Open the 7500 Software Help by clicking
• Amplification efficiency – Refer to the Amplification Efficiency of TaqMan

Set Up the Samples

In the Samples screen, enter the number of samples, replicates, and negative controls to
include in the reaction plate, enter the sample names, then select the sample/target
reactions to set up.
About the
In the relative standard curve example experiment:
Example
• Two samples are used: cDNA prepared from total RNA isolated from liver and
Experiment
• Three replicates are used. The replicates are identical reactions, containing identical
• Three negative controls are used. The negative control reactions contain water
Complete the 1. In the Samples screen, click the How many samples do you want to test in the
Samples Screen
2. Click the How many replicates do you need? field, then enter 3.
3. Click the How many negative controls do you need for each target assay? field,
4. Set up Sample 1:
5. Set up Sample 2:
6. Select No Biological Replicates.
7. Select All Sample/Target Reactions to test all targets in all samples.
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Chapter 2 Design the Relative Standard Curve Experiment
pressing F1.
Expression Assays Application Note.
kidney tissues. The samples contain unknown quantities of the c-myc gene (target)
and GAPDH gene (endogenous control).
reaction components and volumes.
instead of sample and should not amplify.
reaction plate? field, then enter 2.
The number of rows in the samples table is updated with the number you
Note:
entered.
then enter 3.
a. Click the Enter Sample Name field, then enter Liver.
In the Color field, leave the default.
b.
Click the Enter Sample Name field, then enter Kidney.
a.
In the Color field, leave the default.
b.

Set Up the Samples

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Gene
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