View The Multicomponent Plot - abi 7500 Getting Started Manual

View the Multicomponent Plot

The Multicomponent Plot screen displays the complete spectral contribution of each dye
in a selected well over the duration of the PCR run.
About the In the example experiment, you review the Multicomponent Plot screen for:
Example
• ROX
Experiment
• FAM
• Spikes, dips, and/or sudden changes
• Amplification in the negative control wells
View the Plot 1. In the navigation pane, select Analysis
2. Display the unknown and standard wells one at a time in the Multicomponent Plot
3. In the Plot Color drop-down list, select Dye.
4. Click
5. View the FAM dye signal. In the example experiment, the FAM dye signal increases
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Chapter 5 Analyze the Relative Standard Curve Experiment
™
dye (passive reference)
™
dye (reporter)
If no data are displayed, click Analyze.
Note:
screen:
Select the View Plate Layout tab.
a.
b. Select one well in the plate layout; the well is shown in the Multicomponent
Plot screen.
If you select multiple wells, the Multicomponent Plot screen displays the
Note:
data for all selected wells simultaneously.
(Show a legend for the plot).
This is a toggle button. When the legend is displayed, the button changes to
Note:
Hide the plot legend.
throughout the PCR process, which indicates normal amplification.

View the Multicomponent Plot

Multicomponent Plot.
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