Design The Relative Standard Curve Experiment - abi 7500 Getting Started Manual

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Note:
Help from within Applied Biosystems 7500/7500 Fast Real-Time PCR Software v2.0 by
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Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Design the Relative Standard
Chapter Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Create a New Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Define the Experiment Properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Define the Methods and Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Set Up the Targets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Set Up the Standards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Set Up the Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Set Up the Relative Quantitation Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Set Up the Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Review the Reaction Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Order Materials for the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Finish the Design Wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
For more information about any of the topics discussed in this guide, open the
in the toolbar, or selecting Help 7500 Software Help.
Curve Experiment
Chapter 2
19