Example Experiment Workflow - abi 7500 Getting Started Manual

Example Experiment Workflow

This section shows the workflow for the relative standard curve and comparative C
example experiments.
Start Relative Standard Curve Experiment
Design the Experiment (Chapter 2)
1. Create a new experiment.
2. Define the experiment properties.
3. Define the methods and materials.
4. Set up the targets.
5. Set up the standards.
6. Set up the samples.
7. Set up the relative quantitation.
8. Set up the run method.
9. Review the reaction setup.
10.Order materials for the experiment.
11.Finish the Design Wizard.
Prepare the Reactions (Chapter 3)
1. Prepare the template.
2. Prepare the sample dilutions.
3. Prepare the standard dilution series.
4. Prepare the reaction mix for each target assay.
5. Prepare the reaction plate.
Run the Experiment (Chapter 4)
1. Prepare for the run.
2. Enable the notification settings (Optional).
3. Start the run.
4. Monitor the run.
5. Unload the instrument.
(continued on page 18)
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
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Chapter 1 Get Started

Example Experiment Workflow

Start Comparative C (∆∆C
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Design the Experiment (Chapter 6)
1. Create a new experiment.
2. Define the experiment properties.
3. Define the methods and materials.
4. Set up the targets.
5. Set up the samples.
6. Set up the relative quantitation.
7. Set up the run method.
8. Review the reaction setup.
9. Order materials for the experiment.
10.Finish the Design Wizard.
Prepare the Reactions (Chapter 7)
1. Prepare the template.
2. Prepare the sample dilutions.
3. Prepare the reaction mix for each target assay.
4. Prepare the reaction plate.
Run the Experiment (Chapter 8)
1. Prepare for the run.
2. Enable the notification settings (Optional).
3. Start the run.
4. Monitor the run.
5. Unload the instrument.
(continued on page 18)
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) Experiment
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