Define The Methods And Materials - abi 7500 Getting Started Manual

Chapter 2 Design the Relative Standard Curve Experiment

Define the Methods and Materials

Define the Methods and Materials

About the
Example
Experiment
Complete the
Methods and
Materials Screen
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2
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4
Notes
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
In the Methods and Materials screen, select the quantitation method, reagents, ramp
speed, and PCR template to use for the experiment.
In the relative standard curve example experiment:
• The relative standard curve quantitation method is used.
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• TaqMan reagents are used.
• The standard ramp speed is used in the instrument run.
• cDNA (prepared from total RNA isolated from liver and kidney tissues) is the
template type.You must first perform reverse transcription to convert the RNA to
cDNA (see "Prepare the Template" on page 51).
1. In the Methods and Materials screen, select Relative Standard Curve as the
quantitation method.
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2. Select TaqMan Reagents for the reagents.
IMPORTANT! Fast runs cannot be performed on a 7500 system.
3. Select Standard (~ 2 hours to complete a run) for the ramp speed.
4. Select cDNA (complementary DNA) for the template type.
5. Click Next.
and Comparative C Experiments
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