View The Gene Expression Plot And Well Table - abi 7500 Getting Started Manual

Chapter 9 Analyze the Comparative C

View the Gene Expression Plot and Well Table

View the Gene Expression Plot and Well Table

About the
Example
Experiment
Notes
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Experiment
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Displays the results of relative quantitation calculations in the gene expression profile.
Two plots are available:
• RQ vs Target – Groups the relative quantitation (RQ) values by target. Each sample
is plotted for each target. You can display the plot on a linear, log10, Ln, or log2 scale.
• RQ vs Sample – Groups the relative quantitation (RQ) values by sample. Each target
is plotted for each sample.You can display the plot on a linear, log10, Ln, or log2 scale.
The Well Table displays data for each well in the reaction plate, including:
• The sample name, target name, task, and dyes
• The calculated threshold cycle (C
• Flags
In the comparative C example experiment, you review:
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• Each target in the Gene Expression Plot screen for the expression level (or fold
change) of the target sample relative to the reference sample.
• The Well Table to evaluate the precision of the replicate groups.
), normalized fluorescence (Rn), and quantity values
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and Comparative C Experiments
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