Set Up The Targets - abi 7500 Getting Started Manual

Set Up the Targets

In the Targets screen, enter the number of targets you want to quantify in the PCR
reaction plate, then set up the assay for each target.
About the In the comparative C
Example
• Two targets are quantified in the reaction plate.
Experiment
• The Target 1 assay is set up for the endogenous control. For the example experiment
• The Target 2 through 6 assays are set up for the targets you are studying. For the
Complete the 1. Click the How many targets do you want to quantify in the reaction plate? field,
Targets Screen
2. Set up the Target 1 assay:
3. Set up the remaining targets as shown below:
4. Click Next.
Note:
for the gene/assay ID when you order your materials (see "Order Materials for the
Experiment" on page 131).
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
example experiment:
T
the target is human 18S. 18S serves as the endogenous control because its expression
levels tend to be relatively stable.
example experiment, the targets are EGR3, MAOB, OGDH, OSGEP, and SERPING1.
then enter 6.
The number of rows in the target assays table is updated with the number you
Note:
entered.
Click the Enter Target Name cell, then enter 18S.
a.
b. In the Reporter drop-down list, select FAM (default).
In the Quencher drop-down list, select NFQ-MGB (default).
c.
Leave the default in the Color field.
d.
Target Target Name
2 EGR3
3 MAOB
4 OGDH
5 OSGEP
6 SERPING1
For all targets, leave blank the (Optional) Enter Gene Name field. You can search
Chapter 6 Design the Comparative C
Reporter Quencher
FAM NFQ-MGB
FAM NFQ-MGB
FAM NFQ-MGB
FAM NFQ-MGB
FAM NFQ-MGB
Experiment
T

Set Up the Targets

Color
(Use default color)
(Use default color)
(Use default color)
(Use default color)
(Use default color)
119