Omit Wells From The Analysis - abi 7500 Getting Started Manual

Omit Wells from the Analysis

Experimental error may cause some wells to be amplified insufficiently or not at all.
These wells typically produce C
associated replicate wells. If included in the calculations, these outliers can result in
erroneous measurements. To ensure precision, omit the outliers from the analysis.
About the In the comparative C
Example
removed from analysis.
Experiment
Omit Wells 1. In the navigation pane, select Analysis
2. In the Amplification Plot screen, select C
3. Click the upper left corner of the plate layout in the View Plate Layout tab to display
4. Select the View Well Table tab.
5. In the Well Table:
4
5a
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
values that differ significantly from the average for the
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example experiment, there are no outliers. No wells need to be
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If no data are displayed, click Analyze.
Note:
all wells.
In the Group By drop-down list, select Replicate.
a.
Look for any outliers in the replicate group (be sure they are flagged). In the
b.
example experiment, there are no outliers.
Chapter 9 Analyze the Comparative C

Omit Wells from the Analysis

Amplification Plot.
vs Well in the Plot Type drop-down list.
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Experiment
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