Prepare The Template - abi 7500 Getting Started Manual

Prepare the Template

Prepare the template for the PCR reactions using the High-Capacity cDNA Reverse
Transcription Kit.
IMPORTANT!
Reverse Transcription Kit to reverse-transcribe cDNA from total RNA. The TaqMan
Gene Expression Assays are designed to be compatible with the High-Capacity cDNA
Reverse Transcription Kit; other protocols have not been tested for use with the TaqMan
Gene Expression Assays.
About the For the comparative C
Example reverse-transcribed from total RNA samples using the High-Capacity cDNA Reverse
Experiment Transcription Kit.
Required • One of the following Ambion
Materials
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Applied Biosystems recommends that you use the High-Capacity cDNA
example experiment, the template for the PCR reactions is cDNA
T
®
Kit
®
qRT-PCR RNAlater Tissue Collection: RNA Stabilization
Starter Pack Solution
®
RNaseZap Wipes
RT-PCR Grade Water (nuclease-free)
®
Silencer Validated siRNA, Std Purity
Choice of RNA sample preparation products:
• RNAqueous
Note:
Recommended if you are isolating RNA from
cells or tissues that may be difficult to disrupt or
have high RNase activity.
™
• RiboPure
Note:
Recommended if you are:
• Isolating RNA from all tissues, including those
that may be difficult to disrupt, are rich in lipids,
or have high RNase activity, or
• Labeling and amplifying RNA for use on
microarrays.
• TURBO DNA-free
Note:
Recommended if you are using SYBR
Green reagents. If you are using SYBR Green
reagents, use TURBO DNA-free in conjunction with
the RiboPure Kit.
Chapter 7 Prepare the Comparative C

Prepare the Template

starter packs for RNA isolation:
Contents
®
-4PCR Kit
Kit
™
®
Reactions
T
®
Catalog
Number
AM7020
AM9786
AM9935
AM51331
AM1914
AM1924
AM1907
139