Chapter Overview - abi 7500 Getting Started Manual

Real-time pcr system
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Chapter 8 Run the Comparative C

Chapter Overview

Chapter Overview
Example
Experiment
Workflow
Notes
152
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Experiment
T
This chapter explains how to perform a run on the Applied Biosystems 7500/7500 Fast
Real-Time PCR Systems.
1. Prepare for the run.
2. Enable the notification settings (Optional).
3. Start the run.
4. Monitor the run.
5. Unload the instrument.
Start Comparative C
(∆∆C
T
T
Design the Experiment (Chapter 6)
Prepare the Experiment (Chapter 7)
Run the Experiment (Chapter 8)
Analyze the Experiment (Chapter 9)
End Experiment
) Experiment
and Comparative C
T
Experiments

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