Prepare The Template - abi 7500 Getting Started Manual

Prepare the Template

You prepare the template for the PCR reactions (both samples and standards) using the
High-Capacity cDNA Reverse Transcription Kit.
IMPORTANT!
Reverse Transcription Kit to reverse-transcribe cDNA from total RNA. The TaqMan
Expression Assays are compatible with the High-Capacity cDNA Reverse Transcription Kit;
other protocols have not been tested for use with the TaqMan Gene Expression Assays.
About the For the relative standard curve example experiment, the template for the PCR reactions is
Example cDNA reverse-transcribed from total RNA samples using the High-Capacity cDNA
Experiment Reverse Transcription Kit.
Required
• One of the following Ambion
Materials
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Chapter 3 Prepare the Relative Standard Curve Reactions
Applied Biosystems recommends that you use the High-Capacity cDNA
®
Kit
®
qRT-PCR RNAlater Tissue Collection: RNA Stabilization Solution
Starter Pack
®
RNaseZap Wipes
RT-PCR Grade Water (nuclease-free)
®
Silencer Validated siRNA, Std Purity
One of the following RNA sample preparation products:
®
• RNAqueous
Note:
Recommended if you are isolating RNA from cells
or tissues that may be difficult to disrupt or have high
RNase activity.
™
• RiboPure Kit
Note:
Recommended if you are:
– Isolating RNA from all tissues, including those that
may be difficult to disrupt, are rich in lipids, or have
high RNase activity
or
– Labeling and amplifying RNA for use on microarrays.
• TURBO DNA-free
Note:
Recommended if you are using SYBR
reagents. If you are using SYBR Green reagents, use
TURBO DNA-free with the RiboPure Kit.
PCR Starter RT-PCR Grade Water (nuclease-free)
Pack
™
DNAZap
Choice of RNA sample preparation products, as listed
above under the qRT-PCR Starter Pack.

Prepare the Template

starter packs for RNA isolation:
Contents
-4PCR Kit
™
®
Green
®
Gene
Ambion
Catalog
Number
AM7020
AM9786
AM9935
AM51331
AM1914
AM1924
AM1907
AM9935
AM9890
See above
51