View The Multicomponent Plot - abi 7500 Getting Started Manual

Chapter 9 Analyze the Comparative C

View the Multicomponent Plot

Analysis
Guidelines
For More
Information

View the Multicomponent Plot

About the
Example
Experiment
Notes
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Experiment
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When you analyze your own comparative C
groups for outliers. If needed, remove outliers manually using the Well Table:
1. In the navigation pane, select Analysis
If no data are displayed, click Analyze.
Note:
2. In the Plot Type drop-down list of the Amplification Plot screen, select C
3. Select the View Well Table tab.
4. In the Well Table:
In the Group By drop-down list, select Replicate.
a.
b. Look for any outliers in the replicate group (be sure they are flagged).
c. Select the Omit check box next to the outlying well(s).
5. Click Analyze to reanalyze the experiment data with the outlying well(s) removed
from the analysis.
For more information on omitting wells from the analysis, open the 7500 Software Help
by clicking or pressing F1. Within the Help, search for the omit well topics:
1. Select the Search tab.
2. Enter omit well.
3. Click List Topics.
4. Double-click the topics you want to review.
The Multicomponent Plot screen displays the complete spectral contribution of each dye
in a selected well over the duration of the PCR.
In the comparative C example experiment, you review the Multicomponent Plot screen
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for:
™
• ROX dye (passive reference)
™
• FAM dye (reporter)
• Spikes, dips, and/or sudden changes
• Amplification in the negative control wells
experiment, carefully view the replicate
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Amplification Plot.
and Comparative C
vs Well.
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Experiments
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