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Chapter 9 Analyze the Comparative C
Section Overview
Section Overview
About the
Example Study
Notes
188
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Experiment
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The 7500 software can combine the analysis of experiments that use the comparative C
method (∆∆C
) of relative quantitation.
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In a comparative C
study, you can...
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• Specify the endogenous control(s) and
reference sample for the study.
• Select the control type when applicable.
• Set baseline and threshold values and
confidence levels, or set the number of
standard deviations for Comparative C
Min/Max.
• Omit wells individually or together through
their association with replicate groups
(technical or biological).
For more information about methods of calculating relative quantitation, refer to the
Real-Time PCR Systems Reagent Guide.
In this section, you use two files:
• In the first half, you create a comparative C
data, then save it to the experiments folder on your computer.
• In the second half, you view results in a example study file that is installed with the
7500 software. You can find the data file for the example study on your computer at:
<drive>:\Applied Biosystems\<software name>\experiments\
Comparative Ct Study Example.edm
where:
– <drive> is the computer hard drive on which the 7500 software is installed.
– <software name> is the current version of the 7500 software.
The Comparative Ct Study Example.edm file is not designed to demonstrate the
Note:
use of biological replicate groups; an additional example study provided with the 7500
software provides a better example. You can find the alternate study file on your
computer at: <drive>:\Applied Biosystems\<software name>\experiments\
Comparative Ct Study Example (Biological Groups).edm.
You cannot...
• Create, add, or modify samples.
• Create, add, or modify targets.
• Change assay tasks.
Note:
You can perform these operations in
comparative C
experiments.
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example study file that contains setup
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and Comparative C
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Experiments
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