Design The Comparative C Experiment - abi 7500 Getting Started Manual

Design the Comparative C
This chapter covers:
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Note:
Help from within Applied Biosystems 7500/7500 Fast Real-Time PCR Software v2.0 by
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Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
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Chapter Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Create a New Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Define the Experiment Properties . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114
Define the Methods and Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Set Up the Targets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
Set Up the Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
Set Up the Relative Quantitation Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
Set Up the Run Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
Review the Reaction Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Order Materials for the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Finish the Design Wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134
For more information about any of the topics discussed in this guide, open the
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Chapter 6
Experiment
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