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Chapter 7 Prepare the Comparative C
Chapter Overview
Chapter Overview
Example
Experiment
Workflow
Notes
138
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Reactions
T
This chapter explains how to prepare the PCR reactions for the comparative C
example experiment and it provides guidelines for how to prepare the PCR reactions for
your own comparative C
T
1. Prepare the template.
2. Prepare the sample dilutions.
3. Prepare the reaction mix for each target assay.
4. Prepare the reaction plate.
experiment.
Start Comparative C
(∆∆C
T
T
Design the Experiment (Chapter 6)
Prepare the Reactions (Chapter 7)
Run the Experiment (Chapter 8)
Analyze the Experiment (Chapter 9)
End Experiment
) Experiment
and Comparative C
T
(∆∆C
)
T
T
Experiments
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