Chapter Overview; Chapter 3 Prepare The Relative Standard Curve Reactions - abi 7500 Getting Started Manual

Chapter 3 Prepare the Relative Standard Curve Reactions

Chapter Overview

Chapter Overview

Example
Experiment
Workflow
Notes
50
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
This chapter explains how to prepare the PCR reactions for the relative standard curve
example experiment.
1. Prepare the template.
2. Prepare the sample dilutions.
3. Prepare the standard dilution series.
4. Prepare the reaction mix for each target assay.
5. Prepare the reaction plate.
Start Relative Standard Curve Experiment
Design the Experiment (Chapter 2)
Prepare the Reactions (Chapter 3)
Run the Experiment (Chapter 4)
Analyze the Experiment (Chapter 5)
End Experiment
and Comparative C Experiments
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