Set Up The Standards - abi 7500 Getting Started Manual

Set Up the Standards

In the Standards screen, enter the number of points and replicates for all standard curves
in the reaction plate. For each standard curve, enter the starting quantity and select the
serial factor.
About the
In the relative standard curve example experiment:
Example
• One standard curve is set up for the target (c-myc). The standard used for the
Experiment
• One standard curve is set up for the endogenous control (GAPDH). The standard
• For each standard curve:
Complete the 1. In the Standards screen, click the How many points do you need for each
Standards Screen
2. Click the How many replicates do you need for each point? field, then enter 3.
3. Define the range of standard quantities for the c-myc assay:
4. Define the range of standard quantities for the GAPDH assay:
5. Review the Standard Curve Preview pane for each assay. The standard curves have
6. Click Next.
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Chapter 2 Design the Relative Standard Curve Experiment
standard dilution series is a cDNA sample of known quantity prepared from RNA
isolated from lung tissue.
used for the standard dilution series is a cDNA sample of known quantity prepared
from RNA isolated from lung tissue.
– Five points are used in the standard curve.
– Three replicates are used for each point. Replicates are identical reactions,
containing identical reaction components and volumes.
– The starting quantity is 200 ng, and the serial factor is 1:10.
standard curve? field, then enter 5.
a. Click the Enter Starting Quantity field, then enter 200.0.
In the Select Serial Factor drop-down list, select 1:10.
b.
Click the Enter Starting Quantity field, then enter 200.0.
a.
In the Select Serial Factor drop-down list, select 1:10.
b.
the following points: 200, 20, 2, 0.2, and 0.02.

Set Up the Standards

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