Chapter Overview; Chapter 5 Analyze The Relative Standard Curve Experiment - abi 7500 Getting Started Manual

Chapter 5 Analyze the Relative Standard Curve Experiment

Chapter Overview

Chapter Overview

Example
Experiment
Workflow
Notes
76
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
The 7500 software analyzes the data using the relative standard curve quantitation
method. Section 1 of this chapter explains how to review the analyzed data using several
of the analysis screens and how to publish the data. Section 2. If you obtain questionable
results, Section 2 of this chapter explains how to perform some troubleshooting.
The relative standard curve example experiment includes three flagged wells. The
Note:
flags indicate common problems that you may encounter when performing your own
experiments. Procedures are provided for viewing the flags and omitting one of the wells.
Section 1, Review Results:
1. Analyze.
2. View the standard curve.
3. View the amplification plot.
4. View the gene expression plot/results table.
5. Publish the data.
Section 2, Troubleshoot (If Needed):
1. View the analysis settings; adjust the baseline/threshold.
2. View the quality summary.
3. Omit wells.
4. View the multicomponent plot.
5. View the raw data plot.
Start Relative Standard Curve Experiment
Design the Experiment (Chapter 2)
Prepare the Reactions (Chapter 3)
Run the Experiment (Chapter 4)
Analyze the Experiment (Chapter 5)
End Experiment
and Comparative C Experiments
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