Analyze The Experiment - abi 7500 Getting Started Manual

Chapter 9 Analyze the Comparative C

Analyze the Experiment

Analyze the Experiment

About the
Example
Experiment
Analyze the
Example
Experiment
Notes
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Experiment
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The 7500 software analyzes the experiment and displays results in the analysis screens
(for example, the Amplification Plot screen, QC Summary screen, and so on).
For the comparative C example experiment, use the data file that is installed with the
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7500 software. This data file was created with the same design parameters that are
provided in Chapter 6, then run and analyzed on a 7500/7500 Fast instrument.
The data file for the example experiment is on your computer at:
<drive>:\Applied Biosystems\<software name>\experiments\
Comparative Ct Example.eds
where:
• <drive> is the computer hard drive on which the 7500 software is installed.
• <software name> is the current version of the 7500 software.
1. Double-click (7500 software) or select Start All Programs
Applied Biosystems 7500 Software <software name>
where <software name> is the current version of the 7500 software.
2. In the Home screen, click Open.
3. In the Open dialog box, navigate to the experiments folder at:
<drive>:\Applied Biosystems\<software name>\experiments\
4. Double-click Comparative Ct Example.eds to open the example experiment data
file.
The examples folder contains several data files; be sure to select
Note:
Comparative Ct Example.eds.
and Comparative C Experiments
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