Analyze The Experiment - abi 7500 Getting Started Manual

Chapter 5 Analyze the Relative Standard Curve Experiment

Analyze the Experiment

Analyze the Experiment

About the
Example
Experiment
Analyze the
Example
Experiment
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4
Notes
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
The 7500 software analyzes the experiment and displays results in the analysis screens
(for example, the Amplification Plot screen, QC Summary screen, and so on).
For the relative standard curve example experiment, use the data file that is installed with
the 7500 software. The data file was created with the same design parameters that are
provided in Chapter 2, then run and analyzed on a 7500/7500 Fast instrument.
The data file for the example experiment is on your computer at:
<drive>:\Applied Biosystems\<software name>\experiments\
Relative Standard Curve Example.eds
where:
• <drive> is the computer hard drive on which the 7500 software is installed.
• <software name> is the current version of the 7500 software.
1. Double-click (7500 software) or select Start All Programs
Applied Biosystems 7500 Software <software name>
where <software name> is the current version of the 7500 software.
2. In the Home screen, click Open.
3. In the Open dialog box, navigate to the experiments folder at:
<drive>:\Applied Biosystems\<software name>\experiments\
4. Double-click Relative Standard Curve Example.eds to open the example experiment.
The examples folder contains several data files; be sure to select Relative
Note:
Standard Curve Example.eds.
5. Click Analyze. The software analyzes the data using the default analysis settings.
and Comparative C Experiments
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