View The Raw Data Plot - abi 7500 Getting Started Manual

Analysis When you analyze your own relative standard curve experiment, look for:
Guidelines
• Passive reference – The passive reference dye fluorescence level should be
• Reporter dye – The reporter dye fluorescence level should display a flat region
• Any irregularities in the signal – There should not be any spikes, dips, and/or
• Negative control wells – There should not be any amplification in the negative
For More
For more information on the Multicomponent Plot screen, open the 7500 Software Help
Information by clicking

View the Raw Data Plot

The Raw Data Plot screen displays the raw fluorescence (not normalized) for each optical
filter for the selected wells during each cycle of the real-time PCR.
About the In the relative standard curve example experiment, you review the Raw Data Plot screen
Example for a stable increase in signal (no abrupt changes or dips) from the appropriate filter.
Experiment
View the Plot 1. In the navigation pane, select Analysis
2. Display all 96 wells in the Raw Data Plot screen by clicking the upper left corner of
3. Click
Notes
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
and Comparative C Experiments
T
Chapter 5 Analyze the Relative Standard Curve Experiment
relatively constant throughout the PCR process.
corresponding to the baseline, followed by a rapid rise in fluorescence as the
amplification proceeds.
sudden changes in the fluorescence.
control wells.
or pressing F1.
If no data are displayed, click Analyze.
Note:
the plate layout in the View Plate Layout tab.
(Show a legend for the plot).
This is a toggle button. When the legend is displayed, the button changes to
Note:
Hide the plot legend.
The legend displays the color code for each row of the reaction plate. In the
Note:
example shown below, Row A is red, Row B is yellow/green, Row C is green, and so on.

View the Raw Data Plot

Raw Data Plot.
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