View The Amplification Plot - abi 7500 Getting Started Manual

Chapter 9 Analyze the Comparative C

View the Amplification Plot

View the Amplification Plot

About the
Example
Experiment
Notes
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
Experiment
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The Amplification Plot screen displays amplification of all samples in the selected wells.
Three plots are available:
• ∆Rn vs Cycle – ∆Rn is the magnitude of normalized fluorescence generated by the
reporter at each cycle during the PCR amplification. This plot displays ∆Rn as a
function of cycle number. You can use this plot to identify and examine irregular
amplification and to view threshold and baseline values for the run.
• Rn vs Cycle – Rn is the fluorescence from the reporter dye normalized to the
fluorescence from the passive reference. This plot displays Rn as a function of cycle
number. You can use this plot to identify and examine irregular amplification.
• C vs Well – C is the PCR cycle number at which the fluorescence meets the
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threshold in the amplification plot. This plot displays C
position. You can use this plot to locate outlying amplification (outliers).
Each plot can be displayed as a linear or log10 graph.
In the comparative C example experiment, you review each target in the Amplification
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Plot for:
• Correct baseline and threshold values
• Outliers
as a function of well
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and Comparative C Experiments
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