View The Standard Curve - abi 7500 Getting Started Manual

Chapter 5 Analyze the Relative Standard Curve Experiment

View the Standard Curve

View the Standard Curve

About the
Example
Experiment
View the
Standard Curve
Notes
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Relative Standard Curve
The Standard Curve screen displays the standard curve for samples designated as
standards. The 7500 software calculates the quantity of an unknown target from the
standard curve.
In the relative standard curve example experiment, you review the Standard Curve screen
for the following values:
• Slope/amplification efficiency
2
• R value (correlation coefficient)
• C values
T
1. In the navigation pane, select Analysis
Note: If no data are displayed, click Analyze.
2. Display all 96 wells in the Standard Curve screen by clicking the upper left corner of
the plate layout in the View Plate Layout tab.
3. In the Plot Settings tab, select All in the Target drop-down list (default).
4. In the Plot Settings tab, select Default in the Plot Color drop-down list.
5. Click
(Show a legend for the plot).
This is a toggle button. When the legend is displayed, the button changes to
Note:
Hide the plot legend.
6. View the values displayed below the standard curve. In the example experiment, the
values for each target are within the acceptable ranges:
Target Slope
GAPDH -3.506
c-myc -3.696
Standard Curve.
2
Y-Inter R Value
27.226 0.999
23.82 0.998
and Comparative C
Amplification
Efficiency (Eff%)
92.853
86.438
Experiments
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